pycom10g03240

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0002385
Physical Location & Seq
Forward (+)
3592787 .. 3594234
1448 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 630 bp
ATGGCGAGCTCTTTGCAACACTTCTCCAGCCATGTTCGTCTTTCTCGAACCACTATTGTCTCAGGATCCAAACCTTTCTTGGTGGACGCATTTGTCAAGATCAAACCTGTTGAGCATGTAATGGGCTTACCTGCCACAACTGCAACCCAACAAGGACCAGGGTCCTTATCAAGATTTGCCTCCTCCACTCTTTCCCGTGCCCTCAGCACAGCCACTGCAGCAAGTGAGACTCAGACCCAGAAGCTTGAGCGCATTGCCGACGAACTCCTTGACCTCACAAAGCTCGAGAGGCACGACTACTCCATCCTTTTCAGGCTGAAAATGGGCCTCAATCGGTACGGCCCAGCAGTCTCGGGTATTGGCTCGGCATCTTCTGAATCTGGGCCTGCTTCAACAGAATCCAAGGTCGCTGAGAAGACTGCATTTGATATAAAGATTGAGAAATTCGACGCAGCGGCAAAGATCAAGATCATAAAGGAGGTTAGGACTTTCACTGACTTGGGACTGAAGGAGGCCAAAGAGTTGGTGGAGAAGGTCCCTGTTGTGGTGAAGAAAGGGTTAACCAAGGAGGAGGCGGGGCCCATTGTCGACAAGCTTAAGGAGTTGGGTGCCACTGTGGTATTGGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L12

Protein Analysis

210

Amino Acids

22.5

Weight (kDa)

9.32

Isoelectric Point (pI)

25.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L12 PF00542 142 - 208 1.1e-20 Ribosomal protein L7/L12 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 92
Acc36I ACCTGC 1 cut(s) 139
AccB1I GGYRCC 1 cut(s) 608
AccI GTMKAC 1 cut(s) 588
AciI CCGC 2 cut(s) 455, 575
AclWI GGATC 2 cut(s) 60, 73
AcsI RAATTY 1 cut(s) 443
AcuI CTGAAG 1 cut(s) 527
AfaI GTAC 1 cut(s) 338
AfiI CCNNNNNNNGG 1 cut(s) 544
AflII CTTAAG 1 cut(s) 596
AgsI TTSAA 1 cut(s) 393
AjnI CCWGG 1 cut(s) 157
AluBI AGCT 4 cut(s) 9, 244, 283, 595
AluI AGCT 4 cut(s) 9, 244, 283, 595
Alw21I GWGCWC 1 cut(s) 11
Alw26I GTCTC 3 cut(s) 64, 221, 355
AlwI GGATC 2 cut(s) 60, 73
AlwNI CAGNNNCTG 1 cut(s) 215
Ama87I CYCGRG 2 cut(s) 284, 352
AoxI GGCC 5 cut(s) 325, 340, 383, 513, 578
ApaI GGGCCC 1 cut(s) 582
ApeKI GCWGC 2 cut(s) 218, 452
ApoI RAATTY 1 cut(s) 443
AspLEI GCGC 1 cut(s) 252
AspS9I GGNCC 8 cut(s) 155, 162, 325, 341, 383, 535, 578, 579
AsuHPI GGTGA 1 cut(s) 559
AvaI CYCGRG 2 cut(s) 284, 352
AvaII GGWCC 3 cut(s) 155, 162, 535
BaeGI GKGCMC 2 cut(s) 202, 582
BamHI GGATCC 1 cut(s) 65
BanI GGYRCC 1 cut(s) 608
BanII GRGCYC 2 cut(s) 11, 582
BbsI GAAGAC 1 cut(s) 422
Bbv12I GWGCWC 1 cut(s) 11
BbvCI CCTCAGC 1 cut(s) 203
BbvI GCAGC 2 cut(s) 230, 464
BccI CCATC 1 cut(s) 311
BceAI ACGGC 1 cut(s) 355
BcgI CGANNNNNNTGC 1 cut(s) 29
BciT130I CCWGG 1 cut(s) 159
BcoDI GTCTC 3 cut(s) 64, 221, 355
BfmI CTRYAG 1 cut(s) 216
BfrI CTTAAG 1 cut(s) 596
BfuAI ACCTGC 1 cut(s) 139
BisI GCNGC 3 cut(s) 219, 453, 456
BlsI GCNGC 3 cut(s) 220, 454, 457
Bme1390I CCNGG 1 cut(s) 159
Bme18I GGWCC 3 cut(s) 155, 162, 535
BmeT110I CYCGRG 2 cut(s) 284, 352
BmgT120I GGNCC 8 cut(s) 155, 162, 325, 341, 383, 535, 578, 579
BmiI GGNNCC 6 cut(s) 67, 163, 537, 579, 580, 610
BmrFI CCNGG 1 cut(s) 159
BmsI GCATC 1 cut(s) 377
BoxI GACNNNNGTC 1 cut(s) 160
BpiI GAAGAC 1 cut(s) 422
BpmI CTGGAG 1 cut(s) 10
Bpu10I CCTNAGC 1 cut(s) 203
BpuEI CTTGAG 1 cut(s) 266
BsaBI GATNNNNATC 1 cut(s) 467
BsaJI CCNNGG 3 cut(s) 158, 402, 564
BsaXI ACNNNNNCTCC 2 cut(s) 284, 314
Bsc4I CCNNNNNNNGG 1 cut(s) 544
Bse3DI GCAATG 1 cut(s) 252
Bse8I GATNNNNATC 1 cut(s) 467
BseBI CCWGG 1 cut(s) 159
BseDI CCNNGG 3 cut(s) 158, 402, 564
BseGI GGATG 1 cut(s) 303
BseJI GATNNNNATC 1 cut(s) 467
BseLI CCNNNNNNNGG 1 cut(s) 544
BseMI GCAATG 1 cut(s) 252
BseMII CTCAG 4 cut(s) 75, 217, 245, 402
BseRI GAGGAG 2 cut(s) 172, 584
BseSI GKGCMC 2 cut(s) 202, 582
BseXI GCAGC 2 cut(s) 230, 464
BseYI CCCAGC 1 cut(s) 343
BshFI GGCC 5 cut(s) 327, 342, 385, 515, 580
BshNI GGYRCC 1 cut(s) 608
BsiHKAI GWGCWC 1 cut(s) 11
BsiHKCI CYCGRG 2 cut(s) 284, 352
BslFI GGGAC 2 cut(s) 516, 521
BslI CCNNNNNNNGG 1 cut(s) 544
BsmAI GTCTC 3 cut(s) 64, 221, 355
BsmFI GGGAC 2 cut(s) 516, 521
BsnI GGCC 5 cut(s) 327, 342, 385, 515, 580
BsoBI CYCGRG 2 cut(s) 284, 352
Bsp120I GGGCCC 1 cut(s) 578
Bsp1286I GDGCHC 3 cut(s) 11, 202, 582
Bsp143I GATC 4 cut(s) 65, 99, 462, 468
BspACI CCGC 2 cut(s) 455, 575
BspANI GGCC 5 cut(s) 327, 342, 385, 515, 580
BspCNI CTCAG 4 cut(s) 74, 216, 244, 403
BspLI GGNNCC 6 cut(s) 67, 163, 537, 579, 580, 610
BspMAI CTGCAG 1 cut(s) 220
BspMI ACCTGC 1 cut(s) 139
BspPI GGATC 2 cut(s) 60, 73
BspT107I GGYRCC 1 cut(s) 608
BspTI CTTAAG 1 cut(s) 596
BsrDI GCAATG 1 cut(s) 252
BssECI CCNNGG 3 cut(s) 158, 402, 564
BssMI GATC 4 cut(s) 65, 99, 462, 468
BssT1I CCWWGG 2 cut(s) 402, 564
Bst2UI CCWGG 1 cut(s) 159
Bst4CI ACNGT 1 cut(s) 616
BstAFI CTTAAG 1 cut(s) 596
BstC8I GCNNGC 2 cut(s) 7, 387
BstDEI CTNAG 4 cut(s) 61, 203, 231, 411
BstF5I GGATG 1 cut(s) 303
BstHHI GCGC 1 cut(s) 252
BstKTI GATC 4 cut(s) 68, 102, 465, 471
BstMAI GTCTC 3 cut(s) 64, 221, 355
BstMBI GATC 4 cut(s) 65, 99, 462, 468
BstMWI GCNNNNNNNGC 3 cut(s) 140, 218, 289
BstNI CCWGG 1 cut(s) 159
BstNSI RCATGY 1 cut(s) 119
BstPAI GACNNNNGTC 1 cut(s) 160
BstSCI CCNGG 1 cut(s) 157
BstSFI CTRYAG 1 cut(s) 216
BstSLI GKGCMC 2 cut(s) 202, 582
BstV1I GCAGC 2 cut(s) 230, 464
BstV2I GAAGAC 1 cut(s) 422
BstX2I RGATCY 1 cut(s) 65
BstXI CCANNNNNNTGG 1 cut(s) 523
BstYI RGATCY 1 cut(s) 65
BsuRI GGCC 5 cut(s) 327, 342, 385, 515, 580
BtsCI GGATG 1 cut(s) 303
BtsI GCAGTG 1 cut(s) 213
BtsIMutI CAGTG 3 cut(s) 213, 492, 612
BveI ACCTGC 1 cut(s) 139
Cac8I GCNNGC 2 cut(s) 7, 387
CaiI CAGNNNCTG 1 cut(s) 215
CfoI GCGC 1 cut(s) 252
Cfr13I GGNCC 8 cut(s) 155, 162, 325, 341, 383, 535, 578, 579
CseI GACGC 2 cut(s) 95, 458
Csp6I GTAC 1 cut(s) 337
CviAII CATG 2 cut(s) 32, 116
CviQI GTAC 1 cut(s) 337
DdeI CTNAG 4 cut(s) 61, 203, 231, 411
DpnI GATC 4 cut(s) 67, 101, 464, 470
DpnII GATC 4 cut(s) 65, 99, 462, 468
DrdI GACNNNNNNGTC 1 cut(s) 92
DseDI GACNNNNNNGTC 1 cut(s) 92
Ecl136II GAGCTC 1 cut(s) 9
Eco130I CCWWGG 2 cut(s) 402, 564
Eco24I GRGCYC 2 cut(s) 11, 582
Eco47I GGWCC 3 cut(s) 155, 162, 535
Eco53kI GAGCTC 1 cut(s) 9
Eco57I CTGAAG 1 cut(s) 527
Eco88I CYCGRG 2 cut(s) 284, 352
EcoICRI GAGCTC 1 cut(s) 9
EcoO109I RGGNCCY 3 cut(s) 162, 535, 578
EcoRII CCWGG 1 cut(s) 157
EcoT14I CCWWGG 2 cut(s) 402, 564
EcoT38I GRGCYC 2 cut(s) 11, 582
ErhI CCWWGG 2 cut(s) 402, 564
FaeI CATG 2 cut(s) 35, 119
FaiI YATR 4 cut(s) 33, 117, 431, 473
FaqI GGGAC 2 cut(s) 516, 521
FatI CATG 2 cut(s) 31, 115
FauI CCCGC 1 cut(s) 568
FblI GTMKAC 1 cut(s) 588
Fnu4HI GCNGC 3 cut(s) 219, 453, 456
FokI GGATG 1 cut(s) 290
FriOI GRGCYC 2 cut(s) 11, 582
Fsp4HI GCNGC 3 cut(s) 219, 453, 456
GlaI GCGC 1 cut(s) 251
GluI GCNGC 3 cut(s) 219, 453, 456
GsaI CCCAGC 1 cut(s) 347
GsuI CTGGAG 1 cut(s) 10
HaeIII GGCC 5 cut(s) 327, 342, 385, 515, 580
HgaI GACGC 2 cut(s) 95, 458
HhaI GCGC 1 cut(s) 252
Hin1II CATG 2 cut(s) 35, 119
Hin6I GCGC 1 cut(s) 250
HinP1I GCGC 1 cut(s) 250
HincII GTYRAC 2 cut(s) 561, 589
HindII GTYRAC 2 cut(s) 561, 589
HindIII AAGCTT 2 cut(s) 242, 593
HinfI GANTC 3 cut(s) 229, 377, 398
HpaI GTTAAC 1 cut(s) 561
HphI GGTGA 1 cut(s) 559
Hpy166II GTNNAC 3 cut(s) 85, 561, 589
Hpy188I TCNGA 2 cut(s) 234, 376
Hpy188III TCNNGA 6 cut(s) 45, 63, 97, 171, 286, 466
Hpy8I GTNNAC 3 cut(s) 85, 561, 589
Hpy99I CGWCG 2 cut(s) 263, 452
HpyAV CCTTC 2 cut(s) 502, 526
HpyCH4III ACNGT 1 cut(s) 616
HpyCH4V TGCA 4 cut(s) 16, 143, 218, 422
HpyF10VI GCNNNNNNNGC 3 cut(s) 140, 218, 289
HpyF3I CTNAG 4 cut(s) 61, 203, 231, 411
Hsp92II CATG 2 cut(s) 35, 119
HspAI GCGC 1 cut(s) 250
KspAI GTTAAC 1 cut(s) 561
Kzo9I GATC 4 cut(s) 65, 99, 462, 468
Lsp1109I GCAGC 2 cut(s) 230, 464
LweI GCATC 1 cut(s) 377
MalI GATC 4 cut(s) 67, 101, 464, 470
MboI GATC 4 cut(s) 65, 99, 462, 468
MboII GAAGA 3 cut(s) 363, 427, 562
MflI RGATCY 1 cut(s) 65
MhlI GDGCHC 3 cut(s) 11, 202, 582
MluCI AATT 1 cut(s) 443
MlyI GAGTC 1 cut(s) 223
MseI TTAA 2 cut(s) 560, 597
MspA1I CMGCKG 1 cut(s) 455
MspCI CTTAAG 1 cut(s) 596
MspR9I CCNGG 1 cut(s) 159
MvaI CCWGG 1 cut(s) 159
MwoI GCNNNNNNNGC 3 cut(s) 140, 218, 289
NdeII GATC 4 cut(s) 65, 99, 462, 468
NlaIII CATG 2 cut(s) 35, 119
NlaIV GGNNCC 6 cut(s) 67, 163, 537, 579, 580, 610
NmeAIII GCCGAG 1 cut(s) 344
NspI RCATGY 1 cut(s) 119
PaeR7I CTCGAG 1 cut(s) 284
PcsI WCGNNNNNNNCGW 2 cut(s) 43, 291
PfeI GAWTC 2 cut(s) 377, 398
PkrI GCNGC 3 cut(s) 220, 454, 457
PleI GAGTC 1 cut(s) 223
PpsI GAGTC 1 cut(s) 223
PpuMI RGGWCCY 2 cut(s) 162, 535
PshAI GACNNNNGTC 1 cut(s) 160
Psp124BI GAGCTC 1 cut(s) 11
Psp5II RGGWCCY 2 cut(s) 162, 535
Psp6I CCWGG 1 cut(s) 157
PspFI CCCAGC 1 cut(s) 343
PspGI CCWGG 1 cut(s) 157
PspN4I GGNNCC 6 cut(s) 67, 163, 537, 579, 580, 610
PspOMI GGGCCC 1 cut(s) 578
PspPI GGNCC 8 cut(s) 155, 162, 325, 341, 383, 535, 578, 579
PspPPI RGGWCCY 2 cut(s) 162, 535
PstI CTGCAG 1 cut(s) 220
PstNI CAGNNNCTG 1 cut(s) 215
PsuI RGATCY 1 cut(s) 65
RsaI GTAC 1 cut(s) 338
RsaNI GTAC 1 cut(s) 337
SacI GAGCTC 1 cut(s) 11
SalI GTCGAC 1 cut(s) 587
SaqAI TTAA 2 cut(s) 560, 597
SatI GCNGC 3 cut(s) 219, 453, 456
Sau3AI GATC 4 cut(s) 65, 99, 462, 468
Sau96I GGNCC 8 cut(s) 155, 162, 325, 341, 383, 535, 578, 579
SchI GAGTC 1 cut(s) 223
ScrFI CCNGG 1 cut(s) 159
SduI GDGCHC 3 cut(s) 11, 202, 582
SfaNI GCATC 1 cut(s) 377
SfcI CTRYAG 1 cut(s) 216
Sfr274I CTCGAG 1 cut(s) 284
SinI GGWCC 3 cut(s) 155, 162, 535
SlaI CTCGAG 1 cut(s) 284
SmlI CTYRAG 3 cut(s) 245, 284, 596
SmoI CTYRAG 3 cut(s) 245, 284, 596
Sse9I AATT 1 cut(s) 443
SsiI CCGC 2 cut(s) 455, 575
SstI GAGCTC 1 cut(s) 11
StyD4I CCNGG 1 cut(s) 157
StyI CCWWGG 2 cut(s) 402, 564
TaaI ACNGT 1 cut(s) 616
TaqI TCGA 4 cut(s) 46, 285, 447, 588
TasI AATT 1 cut(s) 443
TauI GCSGC 1 cut(s) 458
TfiI GAWTC 2 cut(s) 377, 398
Tru1I TTAA 2 cut(s) 560, 597
Tru9I TTAA 2 cut(s) 560, 597
TscAI CASTG 3 cut(s) 220, 499, 619
TseI GCWGC 2 cut(s) 218, 452
TspRI CASTG 3 cut(s) 220, 499, 619
Vha464I CTTAAG 1 cut(s) 596
VpaK11BI GGWCC 3 cut(s) 155, 162, 535
XapI RAATTY 1 cut(s) 443
XceI RCATGY 1 cut(s) 119
XcmI CCANNNNNNNNNTGG 3 cut(s) 76, 523, 619
XhoI CTCGAG 1 cut(s) 284
XmiI GTMKAC 1 cut(s) 588
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.