pycom10g22450

ABC transporter B family member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0001944
Physical Location & Seq
Forward (+)
24800788 .. 24809052
8265 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3966 bp
ATGGGAAGAATTCACATAATTAATGACGCAGAACATATGAAGGCCAGATCATTTTCTGTTGCTTTTGCATGCGGTCCGTCGGTCTGTCCCCTTCTTCCTCTCTTTCTCACAAATCTCAACAGTTTAAAGGTCATTCAAAGGAATCCCATAGTCATATTCGGTTTTTCTCGTAAATGTGCTTTTAGAAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGATCGTCTTCTTCGAATTAGGGCAACTGCCATGGAAGGAGGAAGCGGAGGAGGAGATGGGGAAGCAGAAGTTAATAATAATAACGGGTCACAAGCGAAAGAAGATCAAGAAAAGCAGAAAGTCGCCTTCTATAAACTCTTCGCGTTTATCTGCGGTGGGGAATGGTCTGTCGCAGCCGCTCATGACTCTTGTGTTTGGTAATCTTATCAACACCTTTGGTTCCACCGATCCTGGTCACATCGTTCCCATGATCTCTAAGGTGTCTCTAAAATTTGTGTACTTGGCCATTGGCACTGGAGCTGCAGCACTTCTACAGGTGGCATGTTGGATGGTGACAGGAGAAAGGCAGGCTGCTAGAATTAGAGGAAAGTACATGAAGGCAATCCTAAGGCAGGACATTGGCTTCTTTGACACTGAAACTAGCACTGGTTTAGAGATCATTGGCAGGATGTCTGGGGACACCATTCTAATTCAAGAAGCCATGGGGGAAAAGGTTGGTGGGGAAATTTATACAACTTTGTTCTACCTTTCTTGGTGGTTTTATGATTGCTTTTGTCAAAGGGTGGCGCCTAGCCATTGTATTGCTGGCTACCATTCCTGCCATTGTCATTGCTGGGGCAACAATGGCTACTATCATGGCGAGAATGTCATCCCGCGGACAGACTGCTTATGCAGAAGCTGGCAATATAGTAGAACAAACAGTTGCTTCAGAGTTGCATCTTTCACTGGGGAGAAGCAAGCAATTGAAAAGTACAACCGGAAGCTAAAAGTTGCTTACAATACTATGGTTCAACAAGGGCTAGTCACAGGGCTTGGACTCGGAATATTTATGCTCGTCATCTTCTGCACGTACGCGCTTGCGGTATGGTATGGATCCAAAATGATCATCAAACATGGATACAATGGAGGCCAAGTTATTAATGTTATATTTGCACTCATGACTGGCGGAATGTCACTCGGTCAGGCATCACCATCGTTGAATGCATTCGCATCAGGGAAAGCTGCTGCATATAAAATGTTTGAGACCATCAGCCGAAATCCAACTATCGATCCCTACGATGCAAGTGGAATTATATTGCAAGATGTTAAAGGTGATGTCGAACTCAAAGACGTGTATTTCAGGTACCCTGCCAGACCAGACGTGAAGATCTTTGTTGGGTTCTCATTATATGTTCCAAGTGGCACAACCACTGCTTTGGTTGGGCAGAGTGGAAGTGGTAAGTCGACAGTGATCGGGCTAGTAGAAAGGTTTTATGATCCAGAAACCGGTGAAGTACTTATAGATGGTGTGGACTTGAAGAAGTTGCAGCTTAAAGCGATAAGGGAGAAGATCGGGCTGGTTAGTCAGGAACCGAACCTCTTTACAACAACTATAAGGGAGAACATAAAATATGGGAAGGATGATGCAACTGATGAGGAGATTAGAAGAGCAACTGAGCTCGCAAATGCAGCAAAATTCATTGATAAGCTACCCCAGGGGATCGACACAATGGTAGGTGAGCATGGGACATCACTATCTGGTGGACAAAAGCAGAGAATTGCAATTGCAAGAGCCATTCTAAAGAACCCGAGAATTCTTCTTCTCGATGAAGCAACAAGCGCACTGGATGCTGAGTCCGAAAAGATTGTTCAAGATGCACTGGTGAACTTGATGTCAAATCGCACAACCATAGTTGTTGCGCATCGCTTGACAACTATTAGGAATGCTGATTGCATAGCAGTGGTGCACAGAGGCAAAATTGTTGAAAAAGGGACTCATAATGAGTTGACCAAAGATCCAGAAGGGGCTTACAGCCAATTAATCCGCCTGCAAGAAGGATCAAAAGTTGACAACGAAGCACAAACGTCTGATCCGAATAGAATGGACACCAGCCTAGATACAGATAGGACTTTGCTCAGCTCTGGGAGCCGGAGATTGTCAATGAGGAGGTCTATAAGCAGAGGCTCATCAGGTAACCGGCACTCATTTACCATTGGCTTTGGCATTCCCGGTCCAGTTGATATCCAAGAGAGTGAAGTAGGACACGAGGATGATCACGACAGACCTAAAGCTGATCCTGAAAAGCGCAAAACAGTTTCTATCCGAAGGCTGGCTGCACTTAACAAACCTGAGGCTCCAGTTTTGCTTATAGGAGCCATTGCTGCAGCAGCGCATGGAGTAATTTTCCCTATCTTTGCGTTACTACTCTCGAAAGCCATTAGAATGTTCTACGAACCGCCCAATGAGCTAAGGCATGATTCCAGAAAATGGGCGTTAGTTTATGTCGGTTTAGGTTGCGCTGCTTTGTTGGTGATTCCTGTGCAGAATTTCTTCTTTGGAGTTGCAGGTGGGAAATTAGTGGAGCGAATTCGTGCCTTGTCATTCCAGAAGGTTGTGCACCAGCAAGTCAGTTGGTTTGATGACCCTGCAAATTCAAGCGGTGCGATTGGTGCAAGGTTATCTACGGATGCTTCAACCATTAAGAGTCTTGTTGGTGATGCCTTAGCGTTGATTGTGCAGAATATAGCAACAATCATCGCAGGGCTGATTATAGGTTTCACAGCTAACTGGAAACTGACGCTGGTAGTTTTAGCCGTTTCGCCGTTGATGCTTCTGCAAGGAACACTTCAGGCAAAATTTCTTAAAGGGTTTAGTGCAGATGCCAAGCTTATGTATGAAGAAGCCAGTCAAGTGGCAAACGATGCGATTGGTAGCATCCGAACGGTTGCATCATTTTGTTCTGAGAAGAAGGTGATGGAGGCATACGAGAAGAAATGTGAGGGTCCGTTGAGGCAAGGAGTACGGTTAGGAGTAGTAAGTGGTTCGGGTTTTGGCTTTTCATTCTTCTTAATGTTCTGCACAAATGCGTTGATATTCTATATTGGAGCTATTCTTGTGAAACATGGTCAAGCTACATTTGAACAAGTATTCAAGGTTTTCTTCGCTGTAACACTCTCTGCAATAGGTGTCTCACAATCTACTAGCATGGCCCCTGACTCTAACAAAGCCAAGGACTCATCGGCTTCGATATTTCAAATTCTCGACAGCAAACCTAAGATTGACTCGAGTAGTAATGAAGGCGTAACGTTATCACATTTAATTGGAGACATTGAGCTTGAGCATGTCAGCTTTAAGTATCCTATGCGCCCGGATGTGCAGATATTCAGAGATATTTGTTTGAAGATGCCATCTGGAAAGTTGAACTGTTGCTTTGGTTGGAGAGAGTGGCAGCGGGAAATCAACGGTAATAGTAGACGGAGTAGAAATCCAAAAGTTCAAGCTAAACTGGCTGAGGCAGCAGATAGGGTTGGTTGGTCAAGAACCAGTTCTCTTCAACGAATCCATTCGTGCCAACATTGCATATGGCAAGCCAGGAGATGTTACCGAGGAAGAAATCATAGCAGCGACCACAGCTACGACACCTCAGTAGGTGAACGAGGGGTTCAACTATCAGGAGGTCAGAAGCAAAGAATTGCCATTGCGAGGGCTATACTCAAAGACCCGAAAATCCTTCTACTTGATGAAGCTACAAGTGCACTGGATGCAGAGTCCGAACGCAAAGTGCAAGACGCATTGGACAGGGTTATGGTGAATAGAACAACAGTTGTAGTTGCGCACCGCCTTAGCACGATAAAAGGAGCAGACATAATCGCGGTGGTGAAGAACGGCGTGATTGCAGAGAAGGGAAGCCATGATTTCCTAATGAAGATCACTGACGGGGCTTATGCTTCTTTGGTGGCACTTAATTCCAGCTCAAATACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko02010 ko04976 ko05206 ko05226 map02010 map04976 map05206 map05226
Pfam Domains
Protein Families
ABC_membrane ABC_tran

Protein Analysis

1322

Amino Acids

145.51

Weight (kDa)

9.25

Isoelectric Point (pI)

34.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_membrane PF00664 123 - 240 1.9e-20 ABC transporter transmembrane region
ABC_membrane PF00664 312 - 389 2.5e-10 ABC transporter transmembrane region
ABC_tran PF00005 461 - 607 3.5e-33 ABC transporter
SMC_N PF02463 488 - 649 1e-05 RecF/RecN/SMC N terminal domain
ABC_membrane PF00664 782 - 1053 3.4e-61 ABC transporter transmembrane region
ABC_tran PF00005 1191 - 1245 1.2e-10 ABC transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2537
Acc16I TGCGCA 2 cut(s) 1902, 3816
Acc36I ACCTGC 1 cut(s) 2537
Acc65I GGTACC 1 cut(s) 1344
AccB1I GGYRCC 2 cut(s) 787, 1344
AccB7I CCANNNNNTGG 1 cut(s) 2469
AccBSI CCGCTC 1 cut(s) 400
AccI GTMKAC 2 cut(s) 1445, 3454
AccII CGCG 4 cut(s) 364, 877, 1076, 3854
AclI AACGTT 1 cut(s) 3287
AcoI YGGCCR 1 cut(s) 504
AcuI CTGAAG 2 cut(s) 913, 2813
AcyI GRCGYC 1 cut(s) 788
AfaI GTAC 7 cut(s) 500, 593, 974, 1073, 1346, 1497, 3003
AfiI CCNNNNNNNGG 5 cut(s) 613, 1487, 2311, 2469, 3684
AflIII ACRYGT 1 cut(s) 1332
AgeI ACCGGT 1 cut(s) 1487
AjiI CACGTC 2 cut(s) 1333, 1363
AjnI CCWGG 3 cut(s) 451, 1695, 3572
Alw21I GWGCWC 4 cut(s) 1662, 1950, 2601, 3739
Alw26I GTCTC 4 cut(s) 489, 1238, 3175, 3300
Alw44I GTGCAC 3 cut(s) 1946, 2597, 3735
AlwNI CAGNNNCTG 1 cut(s) 900
Ama87I CYCGRG 2 cut(s) 1789, 3265
AoxI GGCC 4 cut(s) 42, 504, 1129, 3189
ApaLI GTGCAC 3 cut(s) 1946, 2597, 3735
AseI ATTAAT 3 cut(s) 21, 1140, 2021
AsiGI ACCGGT 1 cut(s) 1487
Asp700I GAANNNNTTC 4 cut(s) 1205, 2531, 3526, 3544
Asp718I GGTACC 1 cut(s) 1344
AspLEI GCGC 9 cut(s) 790, 1078, 1823, 1903, 2289, 2374, 2501, 3348, 3817
AspS9I GGNCC 4 cut(s) 74, 2213, 2984, 3190
AsuC2I CCSGG 2 cut(s) 2211, 3350
AsuII TTCGAA 1 cut(s) 234
AvaI CYCGRG 2 cut(s) 1789, 3265
AvaII GGWCC 3 cut(s) 74, 2213, 2984
AxyI CCTNAGG 2 cut(s) 608, 2331
BaeGI GKGCMC 3 cut(s) 1950, 2601, 3739
BalI TGGCCA 1 cut(s) 506
BamHI GGATCC 1 cut(s) 1094
BanI GGYRCC 2 cut(s) 787, 1344
BanII GRGCYC 1 cut(s) 1662
BauI CACGAG 1 cut(s) 2246
BbsI GAAGAC 1 cut(s) 220
Bbv12I GWGCWC 4 cut(s) 1662, 1950, 2601, 3739
BbvCI CCTCAGC 1 cut(s) 3492
BccI CCATC 7 cut(s) 271, 544, 1201, 1256, 1499, 2950, 3397
BceAI ACGGC 3 cut(s) 2780, 2788, 3883
BcgI CGANNNNNNTGC 2 cut(s) 1176, 1210
BciT130I CCWGG 3 cut(s) 453, 1697, 3574
BciVI GTATCC 2 cut(s) 1112, 3348
BclI TGATCA 2 cut(s) 1104, 2254
BcnI CCSGG 2 cut(s) 2211, 3350
BcoDI GTCTC 4 cut(s) 489, 1238, 3175, 3300
BfaI CTAG 7 cut(s) 576, 642, 792, 1022, 1460, 2096, 3183
BfmI CTRYAG 3 cut(s) 522, 533, 2364
BfoI RGCGCY 1 cut(s) 791
BfuAI ACCTGC 1 cut(s) 2537
BfuI GTATCC 2 cut(s) 1112, 3348
BglII AGATCT 1 cut(s) 1368
BlpI GCTNAGC 1 cut(s) 2117
BmcAI AGTACT 1 cut(s) 1497
Bme1390I CCNGG 5 cut(s) 453, 1697, 2211, 3350, 3574
Bme18I GGWCC 3 cut(s) 74, 2213, 2984
BmeT110I CYCGRG 2 cut(s) 1789, 3265
BmgBI CACGTC 2 cut(s) 1333, 1363
BmgT120I GGNCC 4 cut(s) 74, 2213, 2984, 3190
BmrFI CCNGG 5 cut(s) 453, 1697, 2211, 3350, 3574
BmrI ACTGGG 1 cut(s) 957
BmuI ACTGGG 1 cut(s) 957
BpiI GAAGAC 1 cut(s) 220
BpmI CTGGAG 2 cut(s) 537, 2322
Bpu10I CCTNAGC 4 cut(s) 2450, 2704, 3492, 3824
Bpu1102I GCTNAGC 1 cut(s) 2117
Bpu14I TTCGAA 1 cut(s) 234
BpuEI CTTGAG 1 cut(s) 3338
BpuMI CCSGG 2 cut(s) 2211, 3350
Bsa29I ATCGAT 1 cut(s) 1269
BsaAI YACGTR 1 cut(s) 1071
BsaHI GRCGYC 1 cut(s) 788
BsaI GGTCTC 1 cut(s) 1238
BsaJI CCNNGG 7 cut(s) 251, 702, 875, 1695, 1696, 3210, 3586
BsaWI WCCGGW 2 cut(s) 978, 1487
BsaXI ACNNNNNCTCC 2 cut(s) 1119, 1149
Bsc4I CCNNNNNNNGG 5 cut(s) 613, 1487, 2311, 2469, 3684
Bse118I RCCGGY 2 cut(s) 1487, 2178
Bse21I CCTNAGG 2 cut(s) 608, 2331
Bse3DI GCAATG 4 cut(s) 829, 2358, 3556, 3678
BseBI CCWGG 3 cut(s) 453, 1697, 3574
BseCI ATCGAT 1 cut(s) 1269
BseDI CCNNGG 7 cut(s) 251, 702, 875, 1695, 1696, 3210, 3586
BseLI CCNNNNNNNGG 5 cut(s) 613, 1487, 2311, 2469, 3684
BseMI GCAATG 4 cut(s) 829, 2358, 3556, 3678
BseMII CTCAG 7 cut(s) 1647, 1824, 2131, 2322, 2934, 3483, 3639
BseRI GAGGAG 4 cut(s) 284, 287, 1652, 2161
BseSI GKGCMC 3 cut(s) 1950, 2601, 3739
BseYI CCCAGC 1 cut(s) 834
BsgI GTGCAG 7 cut(s) 1051, 2301, 2543, 2738, 2877, 3043, 3377
Bsh1236I CGCG 4 cut(s) 364, 877, 1076, 3854
BshFI GGCC 4 cut(s) 44, 506, 1131, 3191
BshNI GGYRCC 2 cut(s) 787, 1344
BshTI ACCGGT 1 cut(s) 1487
BshVI ATCGAT 1 cut(s) 1269
BsiHKAI GWGCWC 4 cut(s) 1662, 1950, 2601, 3739
BsiHKCI CYCGRG 2 cut(s) 1789, 3265
BsiSI CCGG 6 cut(s) 979, 1488, 2131, 2179, 2211, 3350
BsiWI CGTACG 1 cut(s) 1071
BslFI GGGAC 4 cut(s) 72, 692, 1741, 1987
BslI CCNNNNNNNGG 5 cut(s) 613, 1487, 2311, 2469, 3684
BsmAI GTCTC 4 cut(s) 489, 1238, 3175, 3300
BsmFI GGGAC 4 cut(s) 72, 692, 1741, 1987
BsmI GAATGC 4 cut(s) 1205, 1207, 1930, 2205
BsnI GGCC 4 cut(s) 44, 506, 1131, 3191
Bso31I GGTCTC 1 cut(s) 1238
BsoBI CYCGRG 2 cut(s) 1789, 3265
Bsp119I TTCGAA 1 cut(s) 234
Bsp1286I GDGCHC 4 cut(s) 1662, 1950, 2601, 3739
Bsp1720I GCTNAGC 1 cut(s) 2117
Bsp19I CCATGG 2 cut(s) 251, 702
BspANI GGCC 4 cut(s) 44, 506, 1131, 3191
BspCNI CTCAG 7 cut(s) 1648, 1825, 2130, 2323, 2935, 3484, 3638
BspDI ATCGAT 1 cut(s) 1269
BspFNI CGCG 4 cut(s) 364, 877, 1076, 3854
BspHI TCATGA 2 cut(s) 402, 1158
BspMAI CTGCAG 2 cut(s) 526, 2368
BspMI ACCTGC 1 cut(s) 2537
BspQI GCTCTTC 1 cut(s) 1642
BspT104I TTCGAA 1 cut(s) 234
BspT107I GGYRCC 2 cut(s) 787, 1344
BspTNI GGTCTC 1 cut(s) 1238
BsrBI CCGCTC 1 cut(s) 400
BsrDI GCAATG 4 cut(s) 829, 2358, 3556, 3678
BsrFI RCCGGY 2 cut(s) 1487, 2178
BssAI RCCGGY 2 cut(s) 1487, 2178
BssECI CCNNGG 7 cut(s) 251, 702, 875, 1695, 1696, 3210, 3586
BssNI GRCGYC 1 cut(s) 788
BssSI CACGAG 1 cut(s) 2246
BssT1I CCWWGG 3 cut(s) 251, 702, 3210
Bst2BI CACGAG 1 cut(s) 2246
Bst2UI CCWGG 3 cut(s) 453, 1697, 3574
Bst4CI ACNGT 9 cut(s) 122, 923, 1450, 2296, 2926, 3006, 3407, 3446, 3805
Bst6I CTCTTC 4 cut(s) 181, 364, 1642, 3537
BstACI GRCGYC 1 cut(s) 788
BstAPI GCANNNNNTGC 3 cut(s) 1829, 2903, 3743
BstBAI YACGTR 1 cut(s) 1071
BstBI TTCGAA 1 cut(s) 234
BstDSI CCRYGG 3 cut(s) 251, 702, 875
BstEII GGTNACC 1 cut(s) 2174
BstENI CCTNNNNNAGG 1 cut(s) 611
BstFNI CGCG 4 cut(s) 364, 877, 1076, 3854
BstH2I RGCGCY 1 cut(s) 791
BstHHI GCGC 9 cut(s) 790, 1078, 1823, 1903, 2289, 2374, 2501, 3348, 3817
BstMAI GTCTC 4 cut(s) 489, 1238, 3175, 3300
BstNI CCWGG 3 cut(s) 453, 1697, 3574
BstNSI RCATGY 3 cut(s) 72, 546, 3326
BstPI GGTNACC 1 cut(s) 2174
BstSCI CCNGG 5 cut(s) 451, 1695, 2209, 3348, 3572
BstSFI CTRYAG 3 cut(s) 522, 533, 2364
BstSLI GKGCMC 3 cut(s) 1950, 2601, 3739
BstUI CGCG 4 cut(s) 364, 877, 1076, 3854
BstV2I GAAGAC 1 cut(s) 220
BstX2I RGATCY 3 cut(s) 1094, 1368, 1996
BstXI CCANNNNNNTGG 2 cut(s) 1417, 2893
BstYI RGATCY 3 cut(s) 1094, 1368, 1996
Bsu15I ATCGAT 1 cut(s) 1269
Bsu36I CCTNAGG 2 cut(s) 608, 2331
BsuI GTATCC 2 cut(s) 1112, 3348
BsuRI GGCC 4 cut(s) 44, 506, 1131, 3191
BsuTUI ATCGAT 1 cut(s) 1269
BtgI CCRYGG 3 cut(s) 251, 702, 875
BtgZI GCGATG 2 cut(s) 1889, 2722
BtrI CACGTC 2 cut(s) 1333, 1363
BtsI GCAGTG 2 cut(s) 1410, 1947
BveI ACCTGC 1 cut(s) 2537
CaiI CAGNNNCTG 1 cut(s) 900
CciI TCATGA 2 cut(s) 402, 1158
CfoI GCGC 9 cut(s) 790, 1078, 1823, 1903, 2289, 2374, 2501, 3348, 3817
Cfr10I RCCGGY 2 cut(s) 1487, 2178
Cfr13I GGNCC 4 cut(s) 74, 2213, 2984, 3190
Cfr42I CCGCGG 1 cut(s) 878
ClaI ATCGAT 1 cut(s) 1269
CpoI CGGWCCG 1 cut(s) 74
CseI GACGC 3 cut(s) 35, 2788, 3779
Csp6I GTAC 7 cut(s) 499, 592, 973, 1072, 1345, 1496, 3002
CspAI ACCGGT 1 cut(s) 1487
CspI CGGWCCG 1 cut(s) 74
CviQI GTAC 7 cut(s) 499, 592, 973, 1072, 1345, 1496, 3002
DinI GGCGCC 1 cut(s) 789
DraI TTTAAA 1 cut(s) 126
EaeI YGGCCR 1 cut(s) 504
Eam1104I CTCTTC 4 cut(s) 181, 364, 1642, 3537
EarI CTCTTC 4 cut(s) 181, 364, 1642, 3537
EciI GGCGGA 2 cut(s) 1182, 2015
Ecl136II GAGCTC 1 cut(s) 1660
Eco130I CCWWGG 3 cut(s) 251, 702, 3210
Eco24I GRGCYC 1 cut(s) 1662
Eco31I GGTCTC 1 cut(s) 1238
Eco32I GATATC 1 cut(s) 2224
Eco47I GGWCC 3 cut(s) 74, 2213, 2984
Eco53kI GAGCTC 1 cut(s) 1660
Eco57I CTGAAG 2 cut(s) 913, 2813
Eco81I CCTNAGG 2 cut(s) 608, 2331
Eco88I CYCGRG 2 cut(s) 1789, 3265
Eco91I GGTNACC 1 cut(s) 2174
EcoICRI GAGCTC 1 cut(s) 1660
EcoNI CCTNNNNNAGG 1 cut(s) 611
EcoO65I GGTNACC 1 cut(s) 2174
EcoRI GAATTC 3 cut(s) 9, 1794, 2568
EcoRII CCWGG 3 cut(s) 451, 1695, 3572
EcoRV GATATC 1 cut(s) 2224
EcoT14I CCWWGG 3 cut(s) 251, 702, 3210
EcoT22I ATGCAT 1 cut(s) 1207
EcoT38I GRGCYC 1 cut(s) 1662
EgeI GGCGCC 1 cut(s) 789
EheI GGCGCC 1 cut(s) 789
ErhI CCWWGG 3 cut(s) 251, 702, 3210
FalI AAGNNNNNCTT 4 cut(s) 2811, 2843, 3125, 3157
FaqI GGGAC 4 cut(s) 72, 692, 1741, 1987
FauI CCCGC 2 cut(s) 882, 3426
FauNDI CATATG 2 cut(s) 36, 3563
FbaI TGATCA 2 cut(s) 1104, 2254
FblI GTMKAC 2 cut(s) 1445, 3454
FriOI GRGCYC 1 cut(s) 1662
FspBI CTAG 7 cut(s) 576, 642, 792, 1022, 1460, 2096, 3183
FspI TGCGCA 2 cut(s) 1902, 3816
GlaI GCGC 9 cut(s) 789, 1077, 1822, 1902, 2288, 2373, 2500, 3347, 3816
GsaI CCCAGC 1 cut(s) 838
GsuI CTGGAG 2 cut(s) 537, 2322
HaeII RGCGCY 1 cut(s) 791
HaeIII GGCC 4 cut(s) 44, 506, 1131, 3191
HapII CCGG 6 cut(s) 979, 1488, 2131, 2179, 2211, 3350
HgaI GACGC 3 cut(s) 35, 2788, 3779
HhaI GCGC 9 cut(s) 790, 1078, 1823, 1903, 2289, 2374, 2501, 3348, 3817
Hin1I GRCGYC 1 cut(s) 788
Hin6I GCGC 9 cut(s) 788, 1076, 1821, 1901, 2287, 2372, 2499, 3346, 3815
HinP1I GCGC 9 cut(s) 788, 1076, 1821, 1901, 2287, 2372, 2499, 3346, 3815
HincII GTYRAC 3 cut(s) 1446, 1989, 2050
HindII GTYRAC 3 cut(s) 1446, 1989, 2050
HindIII AAGCTT 1 cut(s) 2867
HpaII CCGG 6 cut(s) 979, 1488, 2131, 2179, 2211, 3350
Hpy99I CGWCG 1 cut(s) 82
HpyCH4III ACNGT 9 cut(s) 122, 923, 1450, 2296, 2926, 3006, 3407, 3446, 3805
HpyCH4IV ACGT 5 cut(s) 1070, 1332, 1362, 2066, 3287
HpySE526I ACGT 5 cut(s) 1070, 1332, 1362, 2066, 3287
Hsp92I GRCGYC 1 cut(s) 788
HspAI GCGC 9 cut(s) 788, 1076, 1821, 1901, 2287, 2372, 2499, 3346, 3815
KasI GGCGCC 1 cut(s) 787
KpnI GGTACC 1 cut(s) 1348
Ksp22I TGATCA 2 cut(s) 1104, 2254
KspI CCGCGG 1 cut(s) 878
LguI GCTCTTC 1 cut(s) 1642
LmnI GCTCC 7 cut(s) 518, 2127, 2341, 2354, 2563, 3086, 3839
MaeI CTAG 7 cut(s) 576, 642, 792, 1022, 1460, 2096, 3183
MaeII ACGT 5 cut(s) 1070, 1332, 1362, 2066, 3287
MbiI CCGCTC 1 cut(s) 400
MfeI CAATTG 2 cut(s) 963, 1764
MflI RGATCY 3 cut(s) 1094, 1368, 1996
MhlI GDGCHC 4 cut(s) 1662, 1950, 2601, 3739
MlsI TGGCCA 1 cut(s) 506
MluNI TGGCCA 1 cut(s) 506
Mly113I GGCGCC 1 cut(s) 788
MlyI GAGTC 9 cut(s) 400, 1032, 1844, 1969, 2695, 3191, 3209, 3257, 3758
MmeI TCCRAC 3 cut(s) 527, 1286, 3398
Mox20I TGGCCA 1 cut(s) 506
Mph1103I ATGCAT 1 cut(s) 1207
MroXI GAANNNNTTC 4 cut(s) 1205, 2531, 3526, 3544
MscI TGGCCA 1 cut(s) 506
MslI CAYNNNNRTG 3 cut(s) 1940, 2250, 2423
Msp20I TGGCCA 1 cut(s) 506
MspA1I CMGCKG 2 cut(s) 877, 3433
MspI CCGG 6 cut(s) 979, 1488, 2131, 2179, 2211, 3350
MspR9I CCNGG 5 cut(s) 453, 1697, 2211, 3350, 3574
MunI CAATTG 2 cut(s) 963, 1764
Mva1269I GAATGC 4 cut(s) 1205, 1207, 1930, 2205
MvaI CCWGG 3 cut(s) 453, 1697, 3574
MvnI CGCG 4 cut(s) 364, 877, 1076, 3854
NarI GGCGCC 1 cut(s) 788
NciI CCSGG 2 cut(s) 2211, 3350
NcoI CCATGG 2 cut(s) 251, 702
NdeI CATATG 2 cut(s) 36, 3563
NmuCI GTSAC 5 cut(s) 308, 455, 553, 1024, 1173
NsbI TGCGCA 2 cut(s) 1902, 3816
NsiI ATGCAT 1 cut(s) 1207
NspI RCATGY 3 cut(s) 72, 546, 3326
NspV TTCGAA 1 cut(s) 234
PaeI GCATGC 1 cut(s) 72
PaeR7I CTCGAG 1 cut(s) 3265
PagI TCATGA 2 cut(s) 402, 1158
PaqCI CACCTGC 1 cut(s) 2537
PasI CCCWGGG 1 cut(s) 1696
PciSI GCTCTTC 1 cut(s) 1642
PcsI WCGNNNNNNNCGW 3 cut(s) 231, 1275, 2072
PctI GAATGC 4 cut(s) 1205, 1207, 1930, 2205
PdmI GAANNNNTTC 4 cut(s) 1205, 2531, 3526, 3544
PfeI GAWTC 4 cut(s) 142, 2459, 2515, 3540
Pfl23II CGTACG 1 cut(s) 1071
PflMI CCANNNNNTGG 1 cut(s) 2469
PinAI ACCGGT 1 cut(s) 1487
PleI GAGTC 9 cut(s) 400, 1032, 1843, 1969, 2694, 3191, 3209, 3257, 3757
PluTI GGCGCC 1 cut(s) 791
PpsI GAGTC 9 cut(s) 400, 1032, 1843, 1969, 2694, 3191, 3209, 3257, 3757
Ppu21I YACGTR 1 cut(s) 1071
PshBI ATTAAT 3 cut(s) 21, 1140, 2021
Psp124BI GAGCTC 1 cut(s) 1662
Psp1406I AACGTT 1 cut(s) 3287
Psp6I CCWGG 3 cut(s) 451, 1695, 3572
PspEI GGTNACC 1 cut(s) 2174
PspFI CCCAGC 1 cut(s) 834
PspGI CCWGG 3 cut(s) 451, 1695, 3572
PspLI CGTACG 1 cut(s) 1071
PspPI GGNCC 4 cut(s) 74, 2213, 2984, 3190
PspXI VCTCGAGB 1 cut(s) 3265
PstI CTGCAG 2 cut(s) 526, 2368
PstNI CAGNNNCTG 1 cut(s) 900
PsuI RGATCY 3 cut(s) 1094, 1368, 1996
RsaI GTAC 7 cut(s) 500, 593, 974, 1073, 1346, 1497, 3003
RsaNI GTAC 7 cut(s) 499, 592, 973, 1072, 1345, 1496, 3002
RseI CAYNNNNRTG 3 cut(s) 1940, 2250, 2423
Rsr2I CGGWCCG 1 cut(s) 74
RsrII CGGWCCG 1 cut(s) 74
SacI GAGCTC 1 cut(s) 1662
SacII CCGCGG 1 cut(s) 878
SalI GTCGAC 1 cut(s) 1444
SapI GCTCTTC 1 cut(s) 1642
Sau96I GGNCC 4 cut(s) 74, 2213, 2984, 3190
ScaI AGTACT 1 cut(s) 1497
SchI GAGTC 9 cut(s) 400, 1032, 1844, 1969, 2695, 3191, 3209, 3257, 3758
ScrFI CCNGG 5 cut(s) 453, 1697, 2211, 3350, 3574
SduI GDGCHC 4 cut(s) 1662, 1950, 2601, 3739
SfcI CTRYAG 3 cut(s) 522, 533, 2364
SfoI GGCGCC 1 cut(s) 789
Sfr274I CTCGAG 1 cut(s) 3265
Sfr303I CCGCGG 1 cut(s) 878
SfuI TTCGAA 1 cut(s) 234
SgrBI CCGCGG 1 cut(s) 878
SinI GGWCC 3 cut(s) 74, 2213, 2984
SlaI CTCGAG 1 cut(s) 3265
SmiMI CAYNNNNRTG 3 cut(s) 1940, 2250, 2423
SmlI CTYRAG 2 cut(s) 3265, 3317
SmoI CTYRAG 2 cut(s) 3265, 3317
SphI GCATGC 1 cut(s) 72
SspDI GGCGCC 1 cut(s) 787
SspI AATATT 1 cut(s) 1047
SspMI CTAG 7 cut(s) 576, 642, 792, 1022, 1460, 2096, 3183
SstI GAGCTC 1 cut(s) 1662
StyD4I CCNGG 5 cut(s) 451, 1695, 2209, 3348, 3572
StyI CCWWGG 3 cut(s) 251, 702, 3210
TaaI ACNGT 9 cut(s) 122, 923, 1450, 2296, 2926, 3006, 3407, 3446, 3805
TaiI ACGT 5 cut(s) 1073, 1335, 1365, 2069, 3290
TaqII GACCGA 2 cut(s) 70, 1169
TatI WGTACW 4 cut(s) 498, 591, 972, 1495
TauI GCSGC 1 cut(s) 400
TfiI GAWTC 4 cut(s) 142, 2459, 2515, 3540
TseFI GTSAC 5 cut(s) 308, 455, 553, 1024, 1173
Tsp45I GTSAC 5 cut(s) 308, 455, 553, 1024, 1173
TspDTI ATGAA 9 cut(s) 53, 611, 1669, 1824, 2892, 3030, 3291, 3738, 3920
TspGWI ACGGA 4 cut(s) 66, 2681, 2976, 3472
Van91I CCANNNNNTGG 1 cut(s) 2469
VneI GTGCAC 3 cut(s) 1946, 2597, 3735
VpaK11BI GGWCC 3 cut(s) 74, 2213, 2984
VspI ATTAAT 3 cut(s) 21, 1140, 2021
XagI CCTNNNNNAGG 1 cut(s) 611
XceI RCATGY 3 cut(s) 72, 546, 3326
XcmI CCANNNNNNNNNTGG 1 cut(s) 803
XhoI CTCGAG 1 cut(s) 3265
XmiI GTMKAC 2 cut(s) 1445, 3454
XmnI GAANNNNTTC 4 cut(s) 1205, 2531, 3526, 3544
XspI CTAG 7 cut(s) 576, 642, 792, 1022, 1460, 2096, 3183
ZrmI AGTACT 1 cut(s) 1497
Zsp2I ATGCAT 1 cut(s) 1207
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.