pycom10g22470

ABC transporter B family member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0001944
Physical Location & Seq
Reverse (-)
24814791 .. 24819096
4306 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3156 bp
ATGTATGTATTTATAGAAGGGTTTGTTAATTTGTGGCAGGTTGCATCTTTCACTGGGGAGAAGCAAGCAATTGAACAGTACAACCAGAAGCTAAAAGTTGCTTACAATACTATGGTTCATCAAGGGTTAGTCACAGGGATTGGACTCGGAATATTTATGCTTGTCATCTTCTGCTCATACGCGCTTGCAGTATGGTATGGATCCAAAATGATCATCAAACATGGATATAATGGAGGCCAAGTTATCAATGTTATATTTGCACTCATGACTGGTGGAATGTCACTAGGTCAGGCATCACCGTCTTTGAATGCATTCACATCAGGGAAAGCTGCTGCATATAAAATGTTTGAGACCATCAGCCAAAATCCAACTATCGATCGATCCTACGATACAAGTGGAATTGTATTGCAAGATGTTAAAGGTGATGTCGAACTCAAAGACGTGTATTTCAGGTACCCTGCCAGACCAGACGTGCAGATCTTTCCTGAGTTCTCATTATATGTTCCAAGTGGCACATCCACTGCCTTGGTTGGGCAGAGTGGAAGCGGGAAGTCGACAGTGATCGGGCTAGTAGAAAGGTTTTATGATCCAGAAGCCGGTGAAGTACTTATAGATGGCGTGGACTTGAAGAAGTTGCAGCTTAAAGCGATAAGGGAGAAGATCGGGCTGGTTAGTCAGGAACCGAATCTCTTTACAACAACTATAAGGGAGAACATAAAATATGGGAAGGATGATGCAACTGATGAGGAGATTAGAAAAGCAACTGAGCTCGCAAATGCAGCAAAATTCATTGATAAGCTACCCCAGGGGATCGACACAATGGTAGGTGAGAATGGGACATCTCTATCTGGTGGCCAAAAGCAAAGAATTGCAATTGCAAGAGCCATTCTAAAGAACCCGAGAATTCTTCTTCTTGATGAAGCAACAAGCGCTTTGGATGCTGAGTCTGAAAAGATTGTTCAAGATGCACTGGTGAACTTGATGTCAAATTGCACAACCATAGTTGTTGCGCATCGCTTAACAACTATTAGGAATGCTGACTGCATTGCAGTGGTGCACAGAGGAAAAATTGTGGAAAAAGGGACTCATGAGGAGTTGACCAAAGATCCGGAAGGGGATTATAGCCAACTAATCCGCCTGCAAGAAGGATTGAAAGTGGACAACAGAGCACAAACCTCTGATCCGAATAGAGTGGAGACCAGCTTAGATATAGATAGGACCATGCTCAGCTCTGGGAGCCGAAGAATATCAATGAGAAGGTCTATAAGCAAAGGCTCATCAGGTAGCCGGCACTCATTCACTGTTGGCTTTGGCATTCCCGGTCCAATTGATGTCCAAGAGAATGAAGTAGGACACGAGGACGATCATGACAGACCTAAGGTTGATCCCGAAAAACGCAAAACAGTTTCAATCCGAAGGCTGGCCGCCCTTAACATACCTGAGGTTCCAGTTTTGCTTCTAGGAGCCATTGCTGCAGCAGGGCATGGAGTACTTTTCCCGATCTTTGGCTTACTACTTTCGAAAGCCATTGGAATGTTCTACGAACCGCCCAATGAACTAAGGCACGATTCTAGAAAATGGGCGTTAGTTTATGTAGGTTTAGGTTGTGCTGGTCTGTTGGTGGTTCCTGTGCAGAATTTCTTCTTTGGAGTTGCAGGTGGGAAATTGGTGGAGCGAATCCGTGCCTTGTCATTCCAGAAGGTTGTGCACCAGCAAGTCAGTTGGTTTGATGACCCTGCAAATTCAAGCGGTGCCATTGGTGCAAGGTTATCTACGGATGCTTCAACCATTAAGAGTCTTGTTGGTGACGCCTTAGCTTTGATTGTGCAGAACATAGCAACAATCATGGCTGGGCTGGTTATAGGTTTCACAGCTAACTGGAAACTGACGCTCATAGTTTTAGCTGTTTCGCCGTTGATGCTTCTGCAAGGAACACTTCAGGCAAAATTTCTTAAGGGGTTTAGTGCAGATGCCAAGCTTATGTATGAAGAAGCCAGTCAAGTGGCAAACGATGCGATTGGTAGCATCCGAACGGTTGCATCATTTTGTTCTGAGAAGAAGGTGATAGAGGCGTACGAGAAGAAGTGTAAGGGTCCATTGAAACAAGGAGTACGGTTAGGAGTAGTAAGTGGTTTGGGTTTTGGCTTTTCATTCTTCATAATGTTCTGCATAAACGCGTTGGTTTTCTATATTGGAGCTATTTTTGTGAAACATGGTCAAGCTACATTTGAACAAGTATTCAAGGTTTTCTTCGCTATAACAATCAGTGCAATGGGGGTCTCACAAACTACTAGCATGGCTCCTGACTCCAACAAAGCCAAGGACTCAGCAGCTTCGATATTTCGAATTCTCGACAGCAAGCCTAAGATTGACTCGAGCAGTGATGAAGGCATAGCGTTATCACTTTTAATTGGAGAAATCGAGCTTGATCATGTCAACTTTAAGTATCCTACGCGCCCGGATGTGCAGATATTCAGAGACATTTGTTTGAAGATGCCCTCCGGAAAAACTGTTGCTTTGGTTGGAGAGAGTGGCAGCGGGAAGTCAACGGTAATAGGCCTCATAGAGAGATTTTACGATCCTGATTCAGGTCGCGTGCTACTAGACGGAGTAGAAATCCAAAAGTTCAAGCTAAACTGGCTGAGGCAGCAGATAGGGTTGGTTGGTCAAGAACCAGTTCTCTTCAACGAATCCATTCGTGCCAACATTGCATACGGCAAGCCAGGAGATGTTACCGAGGAAGAGATTATAGCAGCGACCACAACAGCTAACGTGCACAATGTCATATCTTCATTGCCTCAAGGCTACAACACGTCAGTAGGTGAACGAGGGGTTCAGCTATCAGGAGGTCAGAAGCAAAGAATCGCCATTGCAAGGGCTATACTCAAGGACCCGAAAATCCTTCTACTTGATGAAGCTACAAGTGCACTGGATGCAGAGTCCGAACGCAAAGTGCAAGACGCATTGGACAGGGTTATGGTGAATAGAACAACAGTTGTGGTTGCGCACCGCCTTAGCACGATAAAAGGAGCAGACATAATTGCGGTGGTGAAGCATGGCGTGATTGCGGAGAAGGGAAACCATGATTTTCTAATGAAGATTACTGACGGGGCTTATGCTTCTTTGGTGGCAATTCATTCCAGCTCAAACACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko02010 ko04976 ko05206 ko05226 map02010 map04976 map05206 map05226
Pfam Domains
Protein Families
ABC_membrane ABC_tran

Protein Analysis

1052

Amino Acids

114.07

Weight (kDa)

7.91

Isoelectric Point (pI)

32.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_membrane PF00664 4 - 91 6e-12 ABC transporter transmembrane region
ABC_tran PF00005 164 - 310 4.7e-33 ABC transporter
SMC_N PF02463 199 - 352 2.8e-06 RecF/RecN/SMC N terminal domain
ABC_membrane PF00664 485 - 758 2.3e-61 ABC transporter transmembrane region
ABC_tran PF00005 826 - 975 4.4e-33 ABC transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1646
Acc16I TGCGCA 2 cut(s) 1011, 3006
Acc36I ACCTGC 2 cut(s) 28, 1646
Acc65I GGTACC 1 cut(s) 453
AccB1I GGYRCC 2 cut(s) 453, 1751
AccI GTMKAC 1 cut(s) 554
AccII CGCG 4 cut(s) 182, 2177, 2455, 2595
AccIII TCCGGA 2 cut(s) 1108, 2501
AciI CCGC 9 cut(s) 546, 1135, 1425, 1547, 1749, 2538, 3010, 3044, 3068
AclWI GGATC 9 cut(s) 195, 208, 375, 581, 818, 1100, 1175, 1379, 2573
AcoI YGGCCR 2 cut(s) 853, 1422
AcsI RAATTY 6 cut(s) 785, 903, 1636, 1741, 1946, 2346
AcuI CTGAAG 1 cut(s) 1922
AcyI GRCGYC 1 cut(s) 1809
AfaI GTAC 6 cut(s) 80, 455, 606, 1491, 2075, 2112
AfeI AGCGCT 1 cut(s) 931
AfiI CCNNNNNNNGG 6 cut(s) 531, 596, 1420, 1505, 2588, 2874
AflII CTTAAG 1 cut(s) 1952
AflIII ACRYGT 3 cut(s) 441, 2175, 2811
AjiI CACGTC 3 cut(s) 442, 472, 2814
AjnI CCWGG 2 cut(s) 804, 2722
Alw21I GWGCWC 6 cut(s) 771, 1059, 1171, 1710, 2778, 2929
Alw26I GTCTC 4 cut(s) 344, 1190, 2284, 2472
Alw44I GTGCAC 4 cut(s) 1055, 1706, 2774, 2925
AlwI GGATC 9 cut(s) 195, 208, 375, 581, 818, 1100, 1175, 1379, 2573
Ama87I CYCGRG 2 cut(s) 898, 2374
Aor13HI TCCGGA 2 cut(s) 1108, 2501
Aor51HI AGCGCT 1 cut(s) 931
AoxI GGCC 4 cut(s) 235, 853, 1422, 2557
ApaLI GTGCAC 4 cut(s) 1055, 1706, 2774, 2925
ApoI RAATTY 6 cut(s) 785, 903, 1636, 1741, 1946, 2346
Asp700I GAANNNNTTC 4 cut(s) 311, 1640, 2676, 2694
Asp718I GGTACC 1 cut(s) 453
AspLEI GCGC 5 cut(s) 184, 932, 1012, 2457, 3007
AspS9I GGNCC 4 cut(s) 1218, 1322, 2093, 2890
AsuC2I CCSGG 2 cut(s) 1320, 2459
AsuII TTCGAA 2 cut(s) 1520, 2344
AvaI CYCGRG 2 cut(s) 898, 2374
AvaII GGWCC 4 cut(s) 1218, 1322, 2093, 2890
AxyI CCTNAGG 2 cut(s) 1377, 1440
BaeGI GKGCMC 4 cut(s) 1059, 1710, 2778, 2929
BalI TGGCCA 1 cut(s) 855
BamHI GGATCC 1 cut(s) 200
BanI GGYRCC 2 cut(s) 453, 1751
BanII GRGCYC 1 cut(s) 771
BauI CACGAG 1 cut(s) 1355
Bbv12I GWGCWC 6 cut(s) 771, 1059, 1171, 1710, 2778, 2929
BbvCI CCTCAGC 1 cut(s) 2642
BccI CCATC 2 cut(s) 362, 608
BceAI ACGGC 2 cut(s) 1897, 2731
BciT130I CCWGG 2 cut(s) 806, 2724
BciVI GTATCC 1 cut(s) 2457
BclI TGATCA 2 cut(s) 210, 2428
BcnI CCSGG 2 cut(s) 1320, 2459
BcoDI GTCTC 4 cut(s) 344, 1190, 2284, 2472
BfaI CTAG 6 cut(s) 284, 569, 1460, 1572, 2292, 2603
BfmI CTRYAG 1 cut(s) 1473
BfoI RGCGCY 1 cut(s) 933
BfrI CTTAAG 1 cut(s) 1952
BfuAI ACCTGC 2 cut(s) 28, 1646
BfuI GTATCC 1 cut(s) 2457
BglII AGATCT 1 cut(s) 477
BlpI GCTNAGC 1 cut(s) 1226
BmcAI AGTACT 2 cut(s) 606, 1491
Bme1390I CCNGG 4 cut(s) 806, 1320, 2459, 2724
Bme18I GGWCC 4 cut(s) 1218, 1322, 2093, 2890
BmeT110I CYCGRG 2 cut(s) 898, 2374
BmgBI CACGTC 3 cut(s) 442, 472, 2814
BmgT120I GGNCC 4 cut(s) 1218, 1322, 2093, 2890
BmrFI CCNGG 4 cut(s) 806, 1320, 2459, 2724
BmrI ACTGGG 1 cut(s) 63
BmuI ACTGGG 1 cut(s) 63
Bpu10I CCTNAGC 3 cut(s) 1813, 2642, 3014
Bpu1102I GCTNAGC 1 cut(s) 1226
Bpu14I TTCGAA 2 cut(s) 1520, 2344
BpuEI CTTGAG 2 cut(s) 2784, 2870
BpuMI CCSGG 2 cut(s) 1320, 2459
Bsa29I ATCGAT 2 cut(s) 375, 379
BsaHI GRCGYC 1 cut(s) 1809
BsaI GGTCTC 3 cut(s) 344, 1190, 2284
BsaJI CCNNGG 5 cut(s) 525, 804, 805, 2319, 2736
BsaWI WCCGGW 2 cut(s) 1108, 2501
BsaXI ACNNNNNCTCC 2 cut(s) 225, 255
Bsc4I CCNNNNNNNGG 6 cut(s) 531, 596, 1420, 1505, 2588, 2874
Bse118I RCCGGY 2 cut(s) 596, 1287
Bse1I ACTGG 9 cut(s) 58, 274, 975, 1448, 1883, 1995, 2642, 2675, 2934
Bse21I CCTNAGG 2 cut(s) 1377, 1440
Bse3DI GCAATG 6 cut(s) 1044, 1467, 2277, 2706, 2792, 2868
BseAI TCCGGA 2 cut(s) 1108, 2501
BseBI CCWGG 2 cut(s) 806, 2724
BseCI ATCGAT 2 cut(s) 375, 379
BseDI CCNNGG 5 cut(s) 525, 804, 805, 2319, 2736
BseGI GGATG 7 cut(s) 515, 736, 943, 1783, 2025, 2467, 2938
BseLI CCNNNNNNNGG 6 cut(s) 531, 596, 1420, 1505, 2588, 2874
BseMI GCAATG 6 cut(s) 1044, 1467, 2277, 2706, 2792, 2868
BseMII CTCAG 8 cut(s) 477, 756, 933, 1240, 1431, 2043, 2340, 2633
BseNI ACTGG 9 cut(s) 58, 274, 975, 1448, 1883, 1995, 2642, 2675, 2934
BseRI GAGGAG 2 cut(s) 761, 1106
BseSI GKGCMC 4 cut(s) 1059, 1710, 2778, 2929
BseYI CCCAGC 1 cut(s) 1850
BsgI GTGCAG 5 cut(s) 494, 1652, 1847, 1986, 2486
Bsh1236I CGCG 4 cut(s) 182, 2177, 2455, 2595
Bsh1285I CGRYCG 1 cut(s) 379
BshFI GGCC 4 cut(s) 237, 855, 1424, 2559
BshNI GGYRCC 2 cut(s) 453, 1751
BshVI ATCGAT 2 cut(s) 375, 379
BsiEI CGRYCG 1 cut(s) 379
BsiHKAI GWGCWC 6 cut(s) 771, 1059, 1171, 1710, 2778, 2929
BsiHKCI CYCGRG 2 cut(s) 898, 2374
BsiSI CCGG 6 cut(s) 597, 1109, 1288, 1320, 2459, 2502
BsiWI CGTACG 1 cut(s) 2073
BslFI GGGAC 2 cut(s) 850, 1096
BslI CCNNNNNNNGG 6 cut(s) 531, 596, 1420, 1505, 2588, 2874
BsmAI GTCTC 4 cut(s) 344, 1190, 2284, 2472
BsmFI GGGAC 2 cut(s) 850, 1096
BsmI GAATGC 4 cut(s) 311, 313, 1039, 1314
BsnI GGCC 4 cut(s) 237, 855, 1424, 2559
Bso31I GGTCTC 3 cut(s) 344, 1190, 2284
BsoBI CYCGRG 2 cut(s) 898, 2374
Bsp119I TTCGAA 2 cut(s) 1520, 2344
Bsp1286I GDGCHC 6 cut(s) 771, 1059, 1171, 1710, 2778, 2929
Bsp13I TCCGGA 2 cut(s) 1108, 2501
Bsp1720I GCTNAGC 1 cut(s) 1226
BspACI CCGC 9 cut(s) 546, 1135, 1425, 1547, 1749, 2538, 3010, 3044, 3068
BspANI GGCC 4 cut(s) 237, 855, 1424, 2559
BspCNI CTCAG 8 cut(s) 478, 757, 934, 1239, 1432, 2044, 2339, 2634
BspDI ATCGAT 2 cut(s) 375, 379
BspEI TCCGGA 2 cut(s) 1108, 2501
BspFNI CGCG 4 cut(s) 182, 2177, 2455, 2595
BspHI TCATGA 3 cut(s) 264, 1087, 1366
BspMAI CTGCAG 1 cut(s) 1477
BspMI ACCTGC 2 cut(s) 28, 1646
BspPI GGATC 9 cut(s) 195, 208, 375, 581, 818, 1100, 1175, 1379, 2573
BspT104I TTCGAA 2 cut(s) 1520, 2344
BspT107I GGYRCC 2 cut(s) 453, 1751
BspTI CTTAAG 1 cut(s) 1952
BspTNI GGTCTC 3 cut(s) 344, 1190, 2284
BsrDI GCAATG 6 cut(s) 1044, 1467, 2277, 2706, 2792, 2868
BsrFI RCCGGY 2 cut(s) 596, 1287
BsrI ACTGG 9 cut(s) 58, 274, 975, 1448, 1883, 1995, 2642, 2675, 2934
BssAI RCCGGY 2 cut(s) 596, 1287
BssECI CCNNGG 5 cut(s) 525, 804, 805, 2319, 2736
BssNI GRCGYC 1 cut(s) 1809
BssSI CACGAG 1 cut(s) 1355
BssT1I CCWWGG 2 cut(s) 525, 2319
Bst2BI CACGAG 1 cut(s) 1355
Bst2UI CCWGG 2 cut(s) 806, 2724
Bst6I CTCTTC 2 cut(s) 2687, 2736
BstACI GRCGYC 1 cut(s) 1809
BstAFI CTTAAG 1 cut(s) 1952
BstAPI GCANNNNNTGC 2 cut(s) 2012, 2933
BstBI TTCGAA 2 cut(s) 1520, 2344
BstC8I GCNNGC 9 cut(s) 66, 186, 771, 1139, 1289, 1422, 2360, 2597, 2720
BstENI CCTNNNNNAGG 1 cut(s) 2586
BstF5I GGATG 7 cut(s) 515, 736, 943, 1783, 2025, 2467, 2938
BstFNI CGCG 4 cut(s) 182, 2177, 2455, 2595
BstH2I RGCGCY 1 cut(s) 933
BstHHI GCGC 5 cut(s) 184, 932, 1012, 2457, 3007
BstMAI GTCTC 4 cut(s) 344, 1190, 2284, 2472
BstMCI CGRYCG 1 cut(s) 379
BstNI CCWGG 2 cut(s) 806, 2724
BstSCI CCNGG 4 cut(s) 804, 1318, 2457, 2722
BstSFI CTRYAG 1 cut(s) 1473
BstSLI GKGCMC 4 cut(s) 1059, 1710, 2778, 2929
BstUI CGCG 4 cut(s) 182, 2177, 2455, 2595
BstX2I RGATCY 3 cut(s) 200, 477, 1105
BstXI CCANNNNNNTGG 2 cut(s) 526, 2002
BstYI RGATCY 3 cut(s) 200, 477, 1105
Bsu15I ATCGAT 2 cut(s) 375, 379
Bsu36I CCTNAGG 2 cut(s) 1377, 1440
BsuI GTATCC 1 cut(s) 2457
BsuRI GGCC 4 cut(s) 237, 855, 1424, 2559
BsuTUI ATCGAT 2 cut(s) 375, 379
BtgZI GCGATG 1 cut(s) 998
BtrI CACGTC 3 cut(s) 442, 472, 2814
BtsCI GGATG 7 cut(s) 515, 736, 943, 1783, 2025, 2467, 2938
BtsI GCAGTG 3 cut(s) 519, 1056, 2386
BtsIMutI CAGTG 9 cut(s) 51, 519, 564, 968, 1056, 1299, 2272, 2386, 2927
BveI ACCTGC 2 cut(s) 28, 1646
Cac8I GCNNGC 9 cut(s) 66, 186, 771, 1139, 1289, 1422, 2360, 2597, 2720
CciI TCATGA 3 cut(s) 264, 1087, 1366
CfoI GCGC 5 cut(s) 184, 932, 1012, 2457, 3007
Cfr10I RCCGGY 2 cut(s) 596, 1287
Cfr13I GGNCC 4 cut(s) 1218, 1322, 2093, 2890
ClaI ATCGAT 2 cut(s) 375, 379
CseI GACGC 3 cut(s) 1817, 1897, 2969
Csp6I GTAC 6 cut(s) 79, 454, 605, 1490, 2074, 2111
CspCI CAANNNNNGTGG 2 cut(s) 2979, 3014
CviQI GTAC 6 cut(s) 79, 454, 605, 1490, 2074, 2111
EaeI YGGCCR 2 cut(s) 853, 1422
Eam1104I CTCTTC 2 cut(s) 2687, 2736
EarI CTCTTC 2 cut(s) 2687, 2736
EciI GGCGGA 1 cut(s) 1124
Ecl136II GAGCTC 1 cut(s) 769
Eco130I CCWWGG 2 cut(s) 525, 2319
Eco147I AGGCCT 1 cut(s) 2559
Eco24I GRGCYC 1 cut(s) 771
Eco31I GGTCTC 3 cut(s) 344, 1190, 2284
Eco47I GGWCC 4 cut(s) 1218, 1322, 2093, 2890
Eco47III AGCGCT 1 cut(s) 931
Eco53kI GAGCTC 1 cut(s) 769
Eco57I CTGAAG 1 cut(s) 1922
Eco81I CCTNAGG 2 cut(s) 1377, 1440
Eco88I CYCGRG 2 cut(s) 898, 2374
EcoICRI GAGCTC 1 cut(s) 769
EcoNI CCTNNNNNAGG 1 cut(s) 2586
EcoO109I RGGNCCY 1 cut(s) 2890
EcoRI GAATTC 2 cut(s) 903, 2346
EcoRII CCWGG 2 cut(s) 804, 2722
EcoT14I CCWWGG 2 cut(s) 525, 2319
EcoT22I ATGCAT 1 cut(s) 313
EcoT38I GRGCYC 1 cut(s) 771
ErhI CCWWGG 2 cut(s) 525, 2319
FalI AAGNNNNNCTT 4 cut(s) 1920, 1952, 2234, 2266
FaqI GGGAC 2 cut(s) 850, 1096
FauI CCCGC 2 cut(s) 539, 2531
FbaI TGATCA 2 cut(s) 210, 2428
FblI GTMKAC 1 cut(s) 554
FokI GGATG 7 cut(s) 502, 743, 950, 1790, 2012, 2474, 2945
FriOI GRGCYC 1 cut(s) 771
FspBI CTAG 6 cut(s) 284, 569, 1460, 1572, 2292, 2603
FspI TGCGCA 2 cut(s) 1011, 3006
GlaI GCGC 5 cut(s) 183, 931, 1011, 2456, 3006
GsaI CCCAGC 1 cut(s) 1854
HaeII RGCGCY 1 cut(s) 933
HaeIII GGCC 4 cut(s) 237, 855, 1424, 2559
HapII CCGG 6 cut(s) 597, 1109, 1288, 1320, 2459, 2502
HgaI GACGC 3 cut(s) 1817, 1897, 2969
HhaI GCGC 5 cut(s) 184, 932, 1012, 2457, 3007
Hin1I GRCGYC 1 cut(s) 1809
Hin6I GCGC 5 cut(s) 182, 930, 1010, 2455, 3005
HinP1I GCGC 5 cut(s) 182, 930, 1010, 2455, 3005
HincII GTYRAC 4 cut(s) 555, 1098, 2437, 2547
HindII GTYRAC 4 cut(s) 555, 1098, 2437, 2547
HindIII AAGCTT 1 cut(s) 1976
HpaII CCGG 6 cut(s) 597, 1109, 1288, 1320, 2459, 2502
HpyCH4IV ACGT 4 cut(s) 441, 471, 2772, 2813
HpySE526I ACGT 4 cut(s) 441, 471, 2772, 2813
Hsp92I GRCGYC 1 cut(s) 1809
HspAI GCGC 5 cut(s) 182, 930, 1010, 2455, 3005
Kpn2I TCCGGA 2 cut(s) 1108, 2501
KpnI GGTACC 1 cut(s) 457
KroI GCCGGC 1 cut(s) 1287
KroNI GCCGGC 1 cut(s) 1289
Ksp22I TGATCA 2 cut(s) 210, 2428
LmnI GCTCC 6 cut(s) 1236, 1463, 1672, 2195, 2305, 3029
MaeI CTAG 6 cut(s) 284, 569, 1460, 1572, 2292, 2603
MaeII ACGT 4 cut(s) 441, 471, 2772, 2813
MaeIII GTNAC 4 cut(s) 130, 279, 1805, 2731
MfeI CAATTG 3 cut(s) 69, 873, 1326
MflI RGATCY 3 cut(s) 200, 477, 1105
MhlI GDGCHC 6 cut(s) 771, 1059, 1171, 1710, 2778, 2929
MlsI TGGCCA 1 cut(s) 855
MluI ACGCGT 1 cut(s) 2175
MluNI TGGCCA 1 cut(s) 855
MlyI GAGTC 8 cut(s) 138, 953, 1078, 1804, 2300, 2318, 2366, 2948
MmeI TCCRAC 3 cut(s) 392, 2334, 2503
Mox20I TGGCCA 1 cut(s) 855
Mph1103I ATGCAT 1 cut(s) 313
MroI TCCGGA 2 cut(s) 1108, 2501
MroNI GCCGGC 1 cut(s) 1287
MroXI GAANNNNTTC 4 cut(s) 311, 1640, 2676, 2694
MscI TGGCCA 1 cut(s) 855
MseI TTAA 9 cut(s) 27, 417, 642, 1019, 1431, 1791, 1953, 2408, 2442
MslI CAYNNNNRTG 2 cut(s) 1049, 1532
Msp20I TGGCCA 1 cut(s) 855
MspA1I CMGCKG 1 cut(s) 2538
MspCI CTTAAG 1 cut(s) 1952
MspI CCGG 6 cut(s) 597, 1109, 1288, 1320, 2459, 2502
MspR9I CCNGG 4 cut(s) 806, 1320, 2459, 2724
MunI CAATTG 3 cut(s) 69, 873, 1326
Mva1269I GAATGC 4 cut(s) 311, 313, 1039, 1314
MvaI CCWGG 2 cut(s) 806, 2724
MvnI CGCG 4 cut(s) 182, 2177, 2455, 2595
NaeI GCCGGC 1 cut(s) 1289
NciI CCSGG 2 cut(s) 1320, 2459
NgoMIV GCCGGC 1 cut(s) 1287
NmuCI GTSAC 3 cut(s) 130, 279, 1805
NsbI TGCGCA 2 cut(s) 1011, 3006
NsiI ATGCAT 1 cut(s) 313
NspV TTCGAA 2 cut(s) 1520, 2344
PaeR7I CTCGAG 1 cut(s) 2374
PagI TCATGA 3 cut(s) 264, 1087, 1366
PaqCI CACCTGC 1 cut(s) 1646
PasI CCCWGGG 1 cut(s) 805
PceI AGGCCT 1 cut(s) 2559
PctI GAATGC 4 cut(s) 311, 313, 1039, 1314
PdiI GCCGGC 1 cut(s) 1289
PdmI GAANNNNTTC 4 cut(s) 311, 1640, 2676, 2694
PfeI GAWTC 6 cut(s) 685, 1568, 1677, 2585, 2690, 2862
Pfl23II CGTACG 1 cut(s) 2073
Ple19I CGATCG 1 cut(s) 379
PleI GAGTC 8 cut(s) 138, 952, 1078, 1803, 2300, 2318, 2366, 2947
PpsI GAGTC 8 cut(s) 138, 952, 1078, 1803, 2300, 2318, 2366, 2947
PpuMI RGGWCCY 1 cut(s) 2890
Psp124BI GAGCTC 1 cut(s) 771
Psp5II RGGWCCY 1 cut(s) 2890
Psp6I CCWGG 2 cut(s) 804, 2722
PspFI CCCAGC 1 cut(s) 1850
PspGI CCWGG 2 cut(s) 804, 2722
PspLI CGTACG 1 cut(s) 2073
PspPI GGNCC 4 cut(s) 1218, 1322, 2093, 2890
PspPPI RGGWCCY 1 cut(s) 2890
PspXI VCTCGAGB 1 cut(s) 2374
PstI CTGCAG 1 cut(s) 1477
PsuI RGATCY 3 cut(s) 200, 477, 1105
PvuI CGATCG 1 cut(s) 379
RsaI GTAC 6 cut(s) 80, 455, 606, 1491, 2075, 2112
RsaNI GTAC 6 cut(s) 79, 454, 605, 1490, 2074, 2111
RseI CAYNNNNRTG 2 cut(s) 1049, 1532
SacI GAGCTC 1 cut(s) 771
SalI GTCGAC 1 cut(s) 553
SaqAI TTAA 9 cut(s) 27, 417, 642, 1019, 1431, 1791, 1953, 2408, 2442
Sau96I GGNCC 4 cut(s) 1218, 1322, 2093, 2890
ScaI AGTACT 2 cut(s) 606, 1491
SchI GAGTC 8 cut(s) 138, 953, 1078, 1804, 2300, 2318, 2366, 2948
ScrFI CCNGG 4 cut(s) 806, 1320, 2459, 2724
SduI GDGCHC 6 cut(s) 771, 1059, 1171, 1710, 2778, 2929
SfcI CTRYAG 1 cut(s) 1473
Sfr274I CTCGAG 1 cut(s) 2374
SfuI TTCGAA 2 cut(s) 1520, 2344
SinI GGWCC 4 cut(s) 1218, 1322, 2093, 2890
SlaI CTCGAG 1 cut(s) 2374
SmiMI CAYNNNNRTG 2 cut(s) 1049, 1532
SmlI CTYRAG 4 cut(s) 1952, 2374, 2799, 2885
SmoI CTYRAG 4 cut(s) 1952, 2374, 2799, 2885
SseBI AGGCCT 1 cut(s) 2559
SsiI CCGC 9 cut(s) 546, 1135, 1425, 1547, 1749, 2538, 3010, 3044, 3068
SspI AATATT 1 cut(s) 153
SspMI CTAG 6 cut(s) 284, 569, 1460, 1572, 2292, 2603
SstI GAGCTC 1 cut(s) 771
StuI AGGCCT 1 cut(s) 2559
StyD4I CCNGG 4 cut(s) 804, 1318, 2457, 2722
StyI CCWWGG 2 cut(s) 525, 2319
TaiI ACGT 4 cut(s) 444, 474, 2775, 2816
TatI WGTACW 3 cut(s) 78, 604, 1489
TauI GCSGC 1 cut(s) 1427
TfiI GAWTC 6 cut(s) 685, 1568, 1677, 2585, 2690, 2862
Tru1I TTAA 9 cut(s) 27, 417, 642, 1019, 1431, 1791, 1953, 2408, 2442
Tru9I TTAA 9 cut(s) 27, 417, 642, 1019, 1431, 1791, 1953, 2408, 2442
TscAI CASTG 9 cut(s) 58, 526, 564, 975, 1056, 1306, 2272, 2386, 2934
TseFI GTSAC 3 cut(s) 130, 279, 1805
Tsp45I GTSAC 3 cut(s) 130, 279, 1805
TspGWI ACGGA 3 cut(s) 1670, 1790, 2622
TspRI CASTG 9 cut(s) 58, 526, 564, 975, 1056, 1306, 2272, 2386, 2934
Vha464I CTTAAG 1 cut(s) 1952
VneI GTGCAC 4 cut(s) 1055, 1706, 2774, 2925
VpaK11BI GGWCC 4 cut(s) 1218, 1322, 2093, 2890
XagI CCTNNNNNAGG 1 cut(s) 2586
XapI RAATTY 6 cut(s) 785, 903, 1636, 1741, 1946, 2346
XbaI TCTAGA 1 cut(s) 1571
XhoI CTCGAG 1 cut(s) 2374
XmiI GTMKAC 1 cut(s) 554
XmnI GAANNNNTTC 4 cut(s) 311, 1640, 2676, 2694
XspI CTAG 6 cut(s) 284, 569, 1460, 1572, 2292, 2603
ZrmI AGTACT 2 cut(s) 606, 1491
Zsp2I ATGCAT 1 cut(s) 313
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.