Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr10

Orthogroup ID

OG0001333

Physical Location & Seq

Genomic Coordinates Reverse (-)

27106634 .. 27110742

Gene Structure 4109 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2553 bp

ATGGCGATTTCTCAGGAGTTGTACCCATCGCAAGACGACCTCCTATACGAAGAGGAGCTTCTCCGCAACCCCTTCAGCCTGAAGCTATGGTGGCGCTACCTGATCGCTCGAGCCGAATCTCCGTTCAAAAAGCGCTTCATAATCTACGAGCGAGCCCTCAAGGCTCTACCCGGAAGCTACAAGCTCTGGTACGCTTACCTCCGCGAACGGCTCGAGCTCGTTCGGAACTTGCCCATCACTCACTCGCAGTACGAAACCCTAATTAACACGTTTGAACGAGCTCTGGTGACGATGCACAAGATGCCCAGGATTTGGATTATGTACTTGCAGACTCTGACGGAGCAGAAATTGGTCACCAAGACCCGCCGGACCTTCGACAGAGCGCTGTGTGCACTTCCTGTGACGCAGCACGACCGCATTTGGGAGCCCTACCTGATGTTTGTGAGCCAAAAGGGCATCCCAATCGAGACCTCGCTTCGGGTTTATCGCAGGTATTTGAAATATGACCCTACCCATATTGAAGATTTCATCGAGTTCTTGATTAATTCTAGTCTTTGGCAAGAGGCTGCCGAGAGGCTTGCTTCGGTGTTGAATGATGATAAGTTTTACTCAATAAAGGGGAAAACGAAACATAGGCTGTGGTTGGAATTGTGTGATTTGCTTACTAAGCATGCCACTGAGGTTTCAGGCCTCAATGTAGACGCTATAATTAGAGGTGGGATTAGGAAGTTTACGGACGAGGTGGGGCGGTTGTGGACCTCTCTTGCGGACTATTACATTAGGAGGAATTTGCACGAGAAGGCGAGGGATATATTTGAGGAAGGTATGACTACTGTGGTAACGGTGAGAGATTTTAGTGTGATTTTTGATTCGTATGCTCATTTTGAAGAGATTGTGCTTGCTCATAAGATGGAAACTGCTGATTTGAGTGATGATGAGGAGGACAAAGAAAATGTGGTGGCAGAGGATAGAAATGAGGAGGAAGAAGATCTTCGATTGGACATTAACTTGTCTGTGGCTGAGCTTGAGAAGAAAATGCTTGATGGGTTTTGGCTACATGAGGATAAGGATGTAGATTTGAGATTAGCTCGATTGGACCATCTCATGGATAGAAGACCTATACTGGCAAATAGTGTTCTTTTACGACAAAATCCTCATAATGTAGAGCAGTGGCACCGGAGAGTGAAGCTATACGAGGGCAATCCCACGAAGCAGATACTTACATACACTGAAGCTGTGAGGACAATTGATCCAATGAAAGCAGTTGGGAAGCCTCACACATTGTGGGTTGCTTTTGCTAAGTTGTACGAGAACCACAATGATATTGTCAATGCCAGAGTGATTTTTGATAAAGCAGTGCAGGTGAATTACAAGACCGTGGATAATTTGGCTAGTCTTTGGTGTGAGTGGGCAGAAATGGAACTGAGGCATAAGAATTTCAAGGGAGCACTGGAACTGATGAGGCGGGCTACTGCAGAGCCATCTGTTGAGGTGAAACGAAGAGTGGCTGCTGATGGGAATGCGCCGGTTCAGATGAAGCTGCATAAATGCTTGAGAATTTGGGCATTTTATGTAGACTTGGAGGAGAGCCTGGGTAAATTAGAGTCCACTCGAGCTGTTTACGAGAGGATATTGGATTTGAAAATAGCTACAACGGAGATCATAATCAATTATGCATCGCTTCTTGAGGAGCATAAATACTTTGAAGATGCATTCAAGGTTTATCAAAAAGGTACTAAGATATTCAAGTATCCACATGTCAAAGACATATGGATTACATATCTTTCCAAATTTGTAAAGAGATATGGGAAGAAAGAACTGGAACGCGCAAGAGAGCTATTTGAGGAGGCTGTTCAAGCGGCTCCTGCCGATTCGAAGAAACCTTTGTATCTTCAATATGCAAAGCTGGAGGAGGATTATGGTCTATCAAAGCGGGCAATGAAGGTCTATGATGAAGCAACTAAGGCTGTTCCAAACCATGAGAAACTGGGCATGTATGAAATCTACATTGCTCGTGCTACAGAGATATTTGGCGTCCCGAAGACGAGGGAAATATACCAGCAGGCCATAGACTCTGGTCTTCCAGACAAAGATGTAAAGACAATGTGTTTAAAGTTTGCTGAGCTTGAGAAGAGTTTGGGAGAAATCGATCGTGCTCGGGCAGTGTATACATATGCTTCACAGTTTTCAGATCCACGATCTGATGTGGACTTCTGGAACAAATGGCATGAGTTTGAGGTGCAACATGGAAATGAAGATACCTTTCGAGAGATGCTTCGAATTAAACGAAGTGTTTCTGCAAGCTATAGCCAGACCCACTTTATTCTACCTGAGTATATGATGCAAAAGGATCAGAAGCTGTACATAGACGAGGCAAAAGACCAGCTGAAACAGGCTGGAGTCCCCGAAGATGAAATGGCTGCCCTAGAGAGGCAGTTGGCACCTGTGGCCAACGATACTACCACTAAAGATAGCAGTAGAATAGGCTTTGTGAGTGCAGGAGCAATTCAACAGACTGACGGAGGGATCGATCAAAGTTGCTACAAATCCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000349 GO:0000375 GO:0000377 GO:0000393 GO:0000398 GO:0000974 GO:0001701 GO:0001824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005681 GO:0005684 GO:0006139 GO:0006259 GO:0006281 GO:0006283 GO:0006289 GO:0006351 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007275 GO:0008150 GO:0008152 GO:0008380 GO:0009058 GO:0009059 GO:0009790 GO:0009792 GO:0009987 GO:0010467 GO:0016043 GO:0016070 GO:0016071 GO:0018130 GO:0019438 GO:0022607 GO:0022613 GO:0022618 GO:0032501 GO:0032502 GO:0032774 GO:0032991 GO:0033554 GO:0034622 GO:0034641 GO:0034645 GO:0034654 GO:0043009 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0048856 GO:0050896 GO:0051716 GO:0065003 GO:0071007 GO:0071010 GO:0071012 GO:0071013 GO:0071014 GO:0071704 GO:0071826 GO:0071840 GO:0090304 GO:0097659 GO:1901360 GO:1901362 GO:1901576 GO:1902494 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko03040 map03040

Pfam Domains

Protein Families

Suf

Protein Analysis

851

Amino Acids

99.76

Weight (kDa)

6.22

Isoelectric Point (pI)

46.56

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HAT_Syf1_CNRKL1_N	PF23233	12 - 170	9.8e-61	Pre-mRNA-splicing factor Syf1/CNRKL1 N-terminal HAT repeat
HAT_PRP39_N	PF23240	15 - 143	1.2e-07	PRP39 N-terminal HAT repeat
HAT_Syf1_M	PF23220	173 - 335	2.1e-58	Pre-mRNA-splicing factor SYF1 middle HAT repeat
HAT_Syf1_M	PF23220	352 - 416	2.5e-24	Pre-mRNA-splicing factor SYF1 middle HAT repeat
HAT_Syf1_CNRKL1_C	PF23231	418 - 812	5.1e-189	Pre-mRNA-splicing factor Syf1/CRNKL1 C-terminal HAT repeat
HAT_PRP39_C	PF23241	517 - 582	1.2e-06	PRP39 C-terminal HAT repeat
HAT_PRP39_N	PF23240	557 - 655	1.5e-08	PRP39 N-terminal HAT repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001333)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G28740
fragaria_vesca	FvH4_3g03910 FvH4_3g04150 FvH4_3g04150 FvH4_3g04150 FvH4_3g04150
malus_domestica	MD05G1324400.v1.1 MD10G1302200.v1.1
prunus_persica	Prupe.5G013200_v2.0.a1
pyrus_communis	pycom05g29860 pycom10g25470
rosa_chinensis	RchiOBHm_Chr5g0006181 RchiOBHm_Chr5g0006191 RchiOBHm_Chr5g0006201 RchiOBHm_Chr5g0006601 RchiOBHm_Chr7g0193971
rosa_laevigata	RLG00000004227 RLG00000031375 RLG00000031376 RLG00000031403
rosa_multiflora	Rmu_co7988368.1_g000001 Rmu_co8257165.1_g000001 Rmu_co8304153.1_g000001 Rmu_co8327759.1_g000001 Rmu_sc0001972.1_g000002 Rmu_sc0002389.1_g000006 Rmu_sc0004331.1_g000002 Rmu_sc0005583.1_g000001 Rmu_sc0009371.1_g000007 Rmu_sc0011154.1_g000005 Rmu_sc0024345.1_g000002 Rmu_sc0032392.1_g000001 Rmu_sc0032393.1_g000001
rosa_roxburghii	Rroxscaffold_1G00069420 Rroxscaffold_1G00069440 Rroxscaffold_1G00069800 Rroxscaffold_2G00122720 Rroxscaffold_3G00261670
rosa_rugosa	Rorug04G0422900
rosa_samantha	Rh5AG050200 Rh5BG046700 Rh5BG046800 Rh5BG049400 Rh5CG055400 Rh5CG055600 Rh5CG057800 Rh5DG046500 Rh5DG048800 Rh7AG139900 Rh7AG140000 Rh7DG141300
rosa_wichuraiana	Rw5G004790 Rw5G005020 Rw7G011940

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1351
Acc36I	ACCTGC	2 cut(s)	480, 1351
AccB1I	GGYRCC	2 cut(s)	1173, 2440
AccB7I	CCANNNNNTGG	2 cut(s)	312, 1105
AccI	GTMKAC	3 cut(s)	699, 1575, 2168
AccII	CGCG	2 cut(s)	204, 1827
AciI	CCGC	9 cut(s)	64, 202, 364, 415, 748, 767, 1465, 1859, 1933
AclWI	GGATC	4 cut(s)	1244, 2186, 2358, 2534
AcoI	YGGCCR	1 cut(s)	2448
AcsI	RAATTY	4 cut(s)	787, 1435, 1557, 1790
AcuI	CTGAAG	3 cut(s)	58, 101, 1251
AcyI	GRCGYC	1 cut(s)	2034
AdeI	CACNNNGTG	1 cut(s)	1284
AfaI	GTAC	7 cut(s)	23, 191, 251, 323, 1307, 1735, 2363
AfeI	AGCGCT	2 cut(s)	134, 384
AfiI	CCNNNNNNNGG	5 cut(s)	312, 421, 477, 1105, 2430
AflIII	ACRYGT	2 cut(s)	267, 1756
AjnI	CCWGG	2 cut(s)	305, 1590
Alw21I	GWGCWC	5 cut(s)	219, 283, 394, 1450, 2158
Alw26I	GTCTC	1 cut(s)	461
Alw44I	GTGCAC	1 cut(s)	390
AlwI	GGATC	4 cut(s)	1244, 2186, 2358, 2534
AlwNI	CAGNNNCTG	2 cut(s)	334, 2359
Ama87I	CYCGRG	4 cut(s)	108, 212, 1611, 2157
Aor51HI	AGCGCT	2 cut(s)	134, 384
AoxI	GGCC	3 cut(s)	688, 2064, 2448
ApaLI	GTGCAC	1 cut(s)	390
ApeKI	GCWGC	5 cut(s)	406, 566, 1508, 1540, 2420
ApoI	RAATTY	4 cut(s)	787, 1435, 1557, 1790
AseI	ATTAAT	1 cut(s)	543
Asp700I	GAANNNNTTC	2 cut(s)	990, 2293
AspLEI	GCGC	5 cut(s)	96, 135, 385, 1525, 1829
AspS9I	GGNCC	3 cut(s)	369, 756, 1096
AsuC2I	CCSGG	1 cut(s)	171
AsuHPI	GGTGA	5 cut(s)	298, 346, 856, 1375, 1504
AsuII	TTCGAA	2 cut(s)	1874, 2278
AvaI	CYCGRG	4 cut(s)	108, 212, 1611, 2157
AvaII	GGWCC	3 cut(s)	369, 756, 1096
BaeGI	GKGCMC	1 cut(s)	394
BaeI	ACNNNNGTAYC	2 cut(s)	1871, 1904
BalI	TGGCCA	1 cut(s)	2450
BanI	GGYRCC	2 cut(s)	1173, 2440
BanII	GRGCYC	4 cut(s)	157, 219, 283, 429
BauI	CACGAG	2 cut(s)	794, 2013
BbsI	GAAGAC	3 cut(s)	1120, 2048, 2072
Bbv12I	GWGCWC	5 cut(s)	219, 283, 394, 1450, 2158
BbvI	GCAGC	5 cut(s)	418, 553, 1495, 1527, 2407
BccI	CCATC	7 cut(s)	34, 242, 904, 1037, 1107, 1489, 1508
BceAI	ACGGC	1 cut(s)	224
BcgI	CGANNNNNNTGC	4 cut(s)	445, 479, 560, 594
BciT130I	CCWGG	2 cut(s)	307, 1592
BciVI	GTATCC	1 cut(s)	1761
BcnI	CCSGG	1 cut(s)	171
BcoDI	GTCTC	1 cut(s)	461
BfaI	CTAG	3 cut(s)	549, 1392, 2426
BfmI	CTRYAG	3 cut(s)	1473, 2019, 2305
BfoI	RGCGCY	3 cut(s)	97, 136, 386
BfuAI	ACCTGC	2 cut(s)	480, 1351
BfuI	GTATCC	1 cut(s)	1761
BglI	GCCNNNNNGGC	2 cut(s)	161, 453
BglII	AGATCT	1 cut(s)	988
BisI	GCNGC	6 cut(s)	407, 567, 1509, 1541, 1860, 2421
BlpI	GCTNAGC	2 cut(s)	1020, 2121
BlsI	GCNGC	6 cut(s)	408, 568, 1510, 1542, 1861, 2422
Bme1390I	CCNGG	3 cut(s)	171, 307, 1592
Bme18I	GGWCC	3 cut(s)	369, 756, 1096
BmeT110I	CYCGRG	4 cut(s)	108, 212, 1611, 2157
BmgT120I	GGNCC	3 cut(s)	369, 756, 1096
BmiI	GGNNCC	4 cut(s)	426, 1175, 1863, 2442
BmrFI	CCNGG	3 cut(s)	171, 307, 1592
BmrI	ACTGGG	1 cut(s)	1997
BmsI	GCATC	7 cut(s)	282, 291, 465, 1685, 1699, 2262, 2331
BmuI	ACTGGG	1 cut(s)	1997
BoxI	GACNNNNGTC	1 cut(s)	2076
BpiI	GAAGAC	3 cut(s)	1120, 2048, 2072
BplI	GAGNNNNNCTC	2 cut(s)	1072, 1104
BpmI	CTGGAG	2 cut(s)	1928, 2418
Bpu1102I	GCTNAGC	2 cut(s)	1020, 2121
Bpu14I	TTCGAA	2 cut(s)	1874, 2278
BpuEI	CTTGAG	5 cut(s)	143, 1046, 1573, 1706, 2147
BpuMI	CCSGG	1 cut(s)	171
Bsa29I	ATCGAT	2 cut(s)	2148, 2529
BsaBI	GATNNNNATC	1 cut(s)	1664
BsaHI	GRCGYC	1 cut(s)	2034
BsaI	GGTCTC	1 cut(s)	461
BsaJI	CCNNGG	3 cut(s)	305, 1377, 1591
BsaWI	WCCGGW	1 cut(s)	1176
Bsc4I	CCNNNNNNNGG	5 cut(s)	312, 421, 477, 1105, 2430
Bse118I	RCCGGY	1 cut(s)	1525
Bse1I	ACTGG	4 cut(s)	1128, 1455, 1824, 1992
Bse3DI	GCAATG	2 cut(s)	1944, 2007
Bse8I	GATNNNNATC	1 cut(s)	1664
BseBI	CCWGG	2 cut(s)	307, 1592
BseCI	ATCGAT	2 cut(s)	2148, 2529
BseDI	CCNNGG	3 cut(s)	305, 1377, 1591
BseGI	GGATG	2 cut(s)	456, 1075
BseJI	GATNNNNATC	1 cut(s)	1664
BseLI	CCNNNNNNNGG	5 cut(s)	312, 421, 477, 1105, 2430
BseMI	GCAATG	2 cut(s)	1944, 2007
BseMII	CTCAG	6 cut(s)	26, 669, 1011, 1415, 2112, 2322
BseNI	ACTGG	4 cut(s)	1128, 1455, 1824, 1992
BseRI	GAGGAG	7 cut(s)	68, 953, 992, 1598, 1703, 1859, 1925
BseSI	GKGCMC	1 cut(s)	394
BseXI	GCAGC	5 cut(s)	418, 553, 1495, 1527, 2407
BsgI	GTGCAG	2 cut(s)	1379, 2517
Bsh1236I	CGCG	2 cut(s)	204, 1827
Bsh1285I	CGRYCG	2 cut(s)	415, 2152
BshFI	GGCC	3 cut(s)	690, 2066, 2450
BshNI	GGYRCC	2 cut(s)	1173, 2440
BshVI	ATCGAT	2 cut(s)	2148, 2529
BsiEI	CGRYCG	2 cut(s)	415, 2152
BsiHKAI	GWGCWC	5 cut(s)	219, 283, 394, 1450, 2158
BsiHKCI	CYCGRG	4 cut(s)	108, 212, 1611, 2157
BsiSI	CCGG	4 cut(s)	171, 367, 1177, 1526
BslFI	GGGAC	2 cut(s)	2021, 2387
BslI	CCNNNNNNNGG	5 cut(s)	312, 421, 477, 1105, 2430
BsmAI	GTCTC	1 cut(s)	461
BsmFI	GGGAC	2 cut(s)	2021, 2387
BsmI	GAATGC	2 cut(s)	1525, 1712
BsnI	GGCC	3 cut(s)	690, 2066, 2450
Bso31I	GGTCTC	1 cut(s)	461
BsoBI	CYCGRG	4 cut(s)	108, 212, 1611, 2157
Bsp119I	TTCGAA	2 cut(s)	1874, 2278
Bsp1286I	GDGCHC	7 cut(s)	157, 219, 283, 394, 429, 1450, 2158
Bsp1407I	TGTACA	1 cut(s)	2361
Bsp1720I	GCTNAGC	2 cut(s)	1020, 2121
BspACI	CCGC	9 cut(s)	64, 202, 364, 415, 748, 767, 1465, 1859, 1933
BspANI	GGCC	3 cut(s)	690, 2066, 2450
BspCNI	CTCAG	6 cut(s)	25, 670, 1012, 1416, 2113, 2323
BspDI	ATCGAT	2 cut(s)	2148, 2529
BspFNI	CGCG	2 cut(s)	204, 1827
BspLI	GGNNCC	4 cut(s)	426, 1175, 1863, 2442
BspMAI	CTGCAG	1 cut(s)	1477
BspMI	ACCTGC	2 cut(s)	480, 1351
BspPI	GGATC	4 cut(s)	1244, 2186, 2358, 2534
BspT104I	TTCGAA	2 cut(s)	1874, 2278
BspT107I	GGYRCC	2 cut(s)	1173, 2440
BspTNI	GGTCTC	1 cut(s)	461
BsrDI	GCAATG	2 cut(s)	1944, 2007
BsrFI	RCCGGY	1 cut(s)	1525
BsrGI	TGTACA	1 cut(s)	2361
BsrI	ACTGG	4 cut(s)	1128, 1455, 1824, 1992
BssAI	RCCGGY	1 cut(s)	1525
BssECI	CCNNGG	3 cut(s)	305, 1377, 1591
BssNAI	GTATAC	1 cut(s)	2169
BssNI	GRCGYC	1 cut(s)	2034
BssSI	CACGAG	2 cut(s)	794, 2013
Bst1107I	GTATAC	1 cut(s)	2169
Bst2BI	CACGAG	2 cut(s)	794, 2013
Bst2UI	CCWGG	2 cut(s)	307, 1592
Bst4CI	ACNGT	4 cut(s)	835, 844, 1378, 2184
Bst6I	CTCTTC	4 cut(s)	45, 882, 1495, 2126
BstACI	GRCGYC	1 cut(s)	2034
BstAPI	GCANNNNNTGC	1 cut(s)	301
BstAUI	TGTACA	1 cut(s)	2361
BstBI	TTCGAA	2 cut(s)	1874, 2278
BstC8I	GCNNGC	8 cut(s)	153, 579, 672, 900, 1467, 1935, 2064, 2302
BstDSI	CCRYGG	1 cut(s)	1377
BstEII	GGTNACC	1 cut(s)	352
BstF5I	GGATG	2 cut(s)	456, 1075
BstFNI	CGCG	2 cut(s)	204, 1827
BstH2I	RGCGCY	3 cut(s)	97, 136, 386
BstHHI	GCGC	5 cut(s)	96, 135, 385, 1525, 1829
BstMAI	GTCTC	1 cut(s)	461
BstMCI	CGRYCG	2 cut(s)	415, 2152
BstNI	CCWGG	2 cut(s)	307, 1592
BstNSI	RCATGY	3 cut(s)	674, 1760, 1996
BstPAI	GACNNNNGTC	1 cut(s)	2076
BstPI	GGTNACC	1 cut(s)	352
BstSCI	CCNGG	3 cut(s)	169, 305, 1590
BstSFI	CTRYAG	3 cut(s)	1473, 2019, 2305
BstSLI	GKGCMC	1 cut(s)	394
BstUI	CGCG	2 cut(s)	204, 1827
BstV1I	GCAGC	5 cut(s)	418, 553, 1495, 1527, 2407
BstV2I	GAAGAC	3 cut(s)	1120, 2048, 2072
BstX2I	RGATCY	2 cut(s)	988, 2191
BstYI	RGATCY	2 cut(s)	988, 2191
BstZ17I	GTATAC	1 cut(s)	2169
Bsu15I	ATCGAT	2 cut(s)	2148, 2529
BsuI	GTATCC	1 cut(s)	1761
BsuRI	GGCC	3 cut(s)	690, 2066, 2450
BsuTUI	ATCGAT	2 cut(s)	2148, 2529
BtgI	CCRYGG	1 cut(s)	1377
BtgZI	GCGATG	2 cut(s)	12, 1662
BtsCI	GGATG	2 cut(s)	456, 1075
BtsI	GCAGTG	3 cut(s)	1175, 1362, 2169
BtsIMutI	CAGTG	6 cut(s)	675, 1175, 1227, 1362, 1448, 2169
BveI	ACCTGC	2 cut(s)	480, 1351
Cac8I	GCNNGC	8 cut(s)	153, 579, 672, 900, 1467, 1935, 2064, 2302
CaiI	CAGNNNCTG	2 cut(s)	334, 2359
CfoI	GCGC	5 cut(s)	96, 135, 385, 1525, 1829
Cfr10I	RCCGGY	1 cut(s)	1525
Cfr13I	GGNCC	3 cut(s)	369, 756, 1096
ClaI	ATCGAT	2 cut(s)	2148, 2529
CseI	GACGC	3 cut(s)	412, 710, 2023
Csp6I	GTAC	7 cut(s)	22, 190, 250, 322, 1306, 1734, 2362
CviAII	CATG	8 cut(s)	671, 1058, 1105, 1757, 1979, 1993, 2228, 2246
CviQI	GTAC	7 cut(s)	22, 190, 250, 322, 1306, 1734, 2362
DraI	TTTAAA	1 cut(s)	2112
DraIII	CACNNNGTG	1 cut(s)	1284
EaeI	YGGCCR	1 cut(s)	2448
Eam1104I	CTCTTC	4 cut(s)	45, 882, 1495, 2126
EarI	CTCTTC	4 cut(s)	45, 882, 1495, 2126
Ecl136II	GAGCTC	2 cut(s)	217, 281
Eco147I	AGGCCT	1 cut(s)	690
Eco24I	GRGCYC	4 cut(s)	157, 219, 283, 429
Eco31I	GGTCTC	1 cut(s)	461
Eco47I	GGWCC	3 cut(s)	369, 756, 1096
Eco47III	AGCGCT	2 cut(s)	134, 384
Eco53kI	GAGCTC	2 cut(s)	217, 281
Eco57I	CTGAAG	3 cut(s)	58, 101, 1251
Eco88I	CYCGRG	4 cut(s)	108, 212, 1611, 2157
Eco91I	GGTNACC	1 cut(s)	352
EcoICRI	GAGCTC	2 cut(s)	217, 281
EcoO65I	GGTNACC	1 cut(s)	352
EcoRII	CCWGG	2 cut(s)	305, 1590
EcoT22I	ATGCAT	2 cut(s)	1678, 1714
EcoT38I	GRGCYC	4 cut(s)	157, 219, 283, 429
FaeI	CATG	8 cut(s)	674, 1061, 1108, 1760, 1982, 1996, 2231, 2249
FalI	AAGNNNNNCTT	4 cut(s)	42, 74, 975, 1007
FaqI	GGGAC	2 cut(s)	2021, 2387
FatI	CATG	8 cut(s)	670, 1057, 1104, 1756, 1978, 1992, 2227, 2245
FauI	CCCGC	3 cut(s)	371, 1458, 1926
FauNDI	CATATG	2 cut(s)	1769, 2173
FblI	GTMKAC	3 cut(s)	699, 1575, 2168
Fnu4HI	GCNGC	6 cut(s)	407, 567, 1509, 1541, 1860, 2421
FokI	GGATG	2 cut(s)	443, 1082
FriOI	GRGCYC	4 cut(s)	157, 219, 283, 429
Fsp4HI	GCNGC	6 cut(s)	407, 567, 1509, 1541, 1860, 2421
FspBI	CTAG	3 cut(s)	549, 1392, 2426
GlaI	GCGC	5 cut(s)	95, 134, 384, 1524, 1828
GluI	GCNGC	6 cut(s)	407, 567, 1509, 1541, 1860, 2421
GsuI	CTGGAG	2 cut(s)	1928, 2418
HaeII	RGCGCY	3 cut(s)	97, 136, 386
HaeIII	GGCC	3 cut(s)	690, 2066, 2450
HapII	CCGG	4 cut(s)	171, 367, 1177, 1526
HgaI	GACGC	3 cut(s)	412, 710, 2023
HhaI	GCGC	5 cut(s)	96, 135, 385, 1525, 1829
Hin1I	GRCGYC	1 cut(s)	2034
Hin1II	CATG	8 cut(s)	674, 1061, 1108, 1760, 1982, 1996, 2231, 2249
Hin6I	GCGC	5 cut(s)	94, 133, 383, 1523, 1827
HinP1I	GCGC	5 cut(s)	94, 133, 383, 1523, 1827
HinfI	GANTC	7 cut(s)	116, 331, 869, 1604, 1871, 2072, 2400
HpaII	CCGG	4 cut(s)	171, 367, 1177, 1526
HphI	GGTGA	5 cut(s)	298, 346, 856, 1375, 1504
Hpy166II	GTNNAC	9 cut(s)	392, 700, 732, 756, 1576, 1608, 1621, 2169, 2209
Hpy188I	TCNGA	6 cut(s)	225, 336, 1533, 2191, 2203, 2355
Hpy188III	TCNNGA	9 cut(s)	14, 466, 538, 1685, 2038, 2084, 2215, 2267, 2550
Hpy8I	GTNNAC	9 cut(s)	392, 700, 732, 756, 1576, 1608, 1621, 2169, 2209
HpyAV	CCTTC	5 cut(s)	82, 382, 793, 815, 1936
HpyCH4III	ACNGT	4 cut(s)	835, 844, 1378, 2184
HpyCH4IV	ACGT	1 cut(s)	269
HpySE526I	ACGT	1 cut(s)	269
Hsp92I	GRCGYC	1 cut(s)	2034
Hsp92II	CATG	8 cut(s)	674, 1061, 1108, 1760, 1982, 1996, 2231, 2249
HspAI	GCGC	5 cut(s)	94, 133, 383, 1523, 1827
LmnI	GCTCC	7 cut(s)	55, 340, 424, 1445, 1690, 1867, 2501
Lsp1109I	GCAGC	5 cut(s)	418, 553, 1495, 1527, 2407
LweI	GCATC	7 cut(s)	282, 291, 465, 1685, 1699, 2262, 2331
MaeI	CTAG	3 cut(s)	549, 1392, 2426
MaeII	ACGT	1 cut(s)	269
MaeIII	GTNAC	4 cut(s)	286, 352, 400, 838
MfeI	CAATTG	1 cut(s)	1245
MflI	RGATCY	2 cut(s)	988, 2191
MhlI	GDGCHC	7 cut(s)	157, 219, 283, 394, 429, 1450, 2158
MlsI	TGGCCA	1 cut(s)	2450
MluNI	TGGCCA	1 cut(s)	2450
MlyI	GAGTC	4 cut(s)	325, 1613, 2066, 2409
MmeI	TCCRAC	1 cut(s)	624
Mox20I	TGGCCA	1 cut(s)	2450
Mph1103I	ATGCAT	2 cut(s)	1678, 1714
MroXI	GAANNNNTTC	2 cut(s)	990, 2293
MscI	TGGCCA	1 cut(s)	2450
MseI	TTAA	5 cut(s)	264, 543, 1005, 2111, 2283
MslI	CAYNNNNRTG	1 cut(s)	2250
Msp20I	TGGCCA	1 cut(s)	2450
MspA1I	CMGCKG	1 cut(s)	2386
MspI	CCGG	4 cut(s)	171, 367, 1177, 1526
MspR9I	CCNGG	3 cut(s)	171, 307, 1592
MunI	CAATTG	1 cut(s)	1245
Mva1269I	GAATGC	2 cut(s)	1525, 1712
MvaI	CCWGG	2 cut(s)	307, 1592
MvnI	CGCG	2 cut(s)	204, 1827
NciI	CCSGG	1 cut(s)	171
NdeI	CATATG	2 cut(s)	1769, 2173
NlaIII	CATG	8 cut(s)	674, 1061, 1108, 1760, 1982, 1996, 2231, 2249
NlaIV	GGNNCC	4 cut(s)	426, 1175, 1863, 2442
NmeAIII	GCCGAG	1 cut(s)	595
NmuCI	GTSAC	3 cut(s)	286, 352, 400
NsiI	ATGCAT	2 cut(s)	1678, 1714
NspI	RCATGY	3 cut(s)	674, 1760, 1996
NspV	TTCGAA	2 cut(s)	1874, 2278
PaeI	GCATGC	1 cut(s)	674
PaeR7I	CTCGAG	3 cut(s)	108, 212, 1611
PaqCI	CACCTGC	1 cut(s)	1351
PceI	AGGCCT	1 cut(s)	690
PciI	ACATGT	1 cut(s)	1756
PcsI	WCGNNNNNNNCGW	3 cut(s)	111, 2284, 2526
PctI	GAATGC	2 cut(s)	1525, 1712
PdmI	GAANNNNTTC	2 cut(s)	990, 2293
PfeI	GAWTC	3 cut(s)	116, 869, 1871
PflMI	CCANNNNNTGG	2 cut(s)	312, 1105
PkrI	GCNGC	6 cut(s)	408, 568, 1510, 1542, 1861, 2422
Ple19I	CGATCG	1 cut(s)	2152
PleI	GAGTC	4 cut(s)	325, 1612, 2066, 2408
PpsI	GAGTC	4 cut(s)	325, 1612, 2066, 2408
PscI	ACATGT	1 cut(s)	1756
PshAI	GACNNNNGTC	1 cut(s)	2076
PshBI	ATTAAT	1 cut(s)	543
Psp124BI	GAGCTC	2 cut(s)	219, 283
Psp6I	CCWGG	2 cut(s)	305, 1590
PspEI	GGTNACC	1 cut(s)	352
PspGI	CCWGG	2 cut(s)	305, 1590
PspN4I	GGNNCC	4 cut(s)	426, 1175, 1863, 2442
PspPI	GGNCC	3 cut(s)	369, 756, 1096
PspXI	VCTCGAGB	3 cut(s)	108, 212, 1611
PstI	CTGCAG	1 cut(s)	1477
PstNI	CAGNNNCTG	2 cut(s)	334, 2359
PsuI	RGATCY	2 cut(s)	988, 2191
PvuI	CGATCG	1 cut(s)	2152
PvuII	CAGCTG	1 cut(s)	2386
RsaI	GTAC	7 cut(s)	23, 191, 251, 323, 1307, 1735, 2363
RsaNI	GTAC	7 cut(s)	22, 190, 250, 322, 1306, 1734, 2362
RseI	CAYNNNNRTG	1 cut(s)	2250
SacI	GAGCTC	2 cut(s)	219, 283
SaqAI	TTAA	5 cut(s)	264, 543, 1005, 2111, 2283
SatI	GCNGC	6 cut(s)	407, 567, 1509, 1541, 1860, 2421
Sau96I	GGNCC	3 cut(s)	369, 756, 1096
SchI	GAGTC	4 cut(s)	325, 1613, 2066, 2409
ScrFI	CCNGG	3 cut(s)	171, 307, 1592
SduI	GDGCHC	7 cut(s)	157, 219, 283, 394, 429, 1450, 2158
SfaNI	GCATC	7 cut(s)	282, 291, 465, 1685, 1699, 2262, 2331
SfcI	CTRYAG	3 cut(s)	1473, 2019, 2305
Sfr274I	CTCGAG	3 cut(s)	108, 212, 1611
SfuI	TTCGAA	2 cut(s)	1874, 2278
SinI	GGWCC	3 cut(s)	369, 756, 1096
SlaI	CTCGAG	3 cut(s)	108, 212, 1611
SmiMI	CAYNNNNRTG	1 cut(s)	2250
SmlI	CTYRAG	8 cut(s)	108, 158, 212, 1025, 1552, 1611, 1685, 2126
SmoI	CTYRAG	8 cut(s)	108, 158, 212, 1025, 1552, 1611, 1685, 2126
SphI	GCATGC	1 cut(s)	674
SseBI	AGGCCT	1 cut(s)	690
SsiI	CCGC	9 cut(s)	64, 202, 364, 415, 748, 767, 1465, 1859, 1933
SspMI	CTAG	3 cut(s)	549, 1392, 2426
SstI	GAGCTC	2 cut(s)	219, 283
StuI	AGGCCT	1 cut(s)	690
StyD4I	CCNGG	3 cut(s)	169, 305, 1590
TaaI	ACNGT	4 cut(s)	835, 844, 1378, 2184
TaiI	ACGT	1 cut(s)	272
TatI	WGTACW	2 cut(s)	321, 2361
TauI	GCSGC	1 cut(s)	1862
TfiI	GAWTC	3 cut(s)	116, 869, 1871
Tru1I	TTAA	5 cut(s)	264, 543, 1005, 2111, 2283
Tru9I	TTAA	5 cut(s)	264, 543, 1005, 2111, 2283
TscAI	CASTG	6 cut(s)	682, 1175, 1234, 1362, 1455, 2169
TseFI	GTSAC	3 cut(s)	286, 352, 400
TseI	GCWGC	5 cut(s)	406, 566, 1508, 1540, 2420
Tsp45I	GTSAC	3 cut(s)	286, 352, 400
TspDTI	ATGAA	9 cut(s)	127, 517, 1271, 1550, 1955, 1968, 2013, 2268, 2427
TspGWI	ACGGA	5 cut(s)	111, 353, 749, 1670, 2535
TspRI	CASTG	6 cut(s)	682, 1175, 1234, 1362, 1455, 2169
Van91I	CCANNNNNTGG	2 cut(s)	312, 1105
VneI	GTGCAC	1 cut(s)	390
VpaK11BI	GGWCC	3 cut(s)	369, 756, 1096
VspI	ATTAAT	1 cut(s)	543
XapI	RAATTY	4 cut(s)	787, 1435, 1557, 1790
XceI	RCATGY	3 cut(s)	674, 1760, 1996
XhoI	CTCGAG	3 cut(s)	108, 212, 1611
XmiI	GTMKAC	3 cut(s)	699, 1575, 2168
XmnI	GAANNNNTTC	2 cut(s)	990, 2293
XspI	CTAG	3 cut(s)	549, 1392, 2426
Zsp2I	ATGCAT	2 cut(s)	1678, 1714

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.