pycom11g22270

MATE efflux family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0000715
Physical Location & Seq
Reverse (-)
25276504 .. 25279001
2498 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1383 bp
ATGGAGAACGGAGACATGGTAGCAAACACTAACAATACGACCCCGTTGTTGGACGCAGGTGATCACGAAGAGGGGTGGTGGAACAAGGTGCTGGACTTAGAGGAGGCAAAAAACCAGGTGTTGTTTTCGTTGCCGATGATTCTGACCAACGTTTTCTTTTACATGATCCCTCTGATTTCTGTCATGTTTGCCGGCCATCTCGGTGAGCTTCAGCTTGCCGGTGCAACCCTTGCAAATTCTTGGGCCACCGTCACCGGCTTCGCTTTCATGGTTGGATCAAGTGGAGCTCTAGAAACGTTGTGTGGGCAAGGATTCGGCGCAAAGTTATATAGAATGCTGGGGATTTATCTACAAGCCTCTTGCATCATTTCATTCCTGTCCTCTATCGCTGTATCCGTAGTCTGGTTCTACACAGAGCCAATACTTATCTTAATTCATCAAGACCCTCAAATTTCAAAATATGCAGGGCTTTATATGAAATTTCTTATCCCGGGATTGTTTGCATACGGCTTCCTGCACAACATTTTGAGATTTCTTCAGACGCAATCTGTTGTCATGCCACTGGTCTACTTCTCGGTGATCCCAGTGGTTCTTCATGTTGGAATTGCATATAGTCTGGTACATTGGACAGGTCTTGGTTATGTGGGAGCTCCGCTGGCAGCTTCGATTTCAATATGGATATCGATGCTAATGTTGGCCACGTACGTTTTGTGCGCAAAGAAGTTTGAGGTTACGTGGGAAGGGTTTTCATTAGAATCATTTCATTATGTCCTCTCCAACTTGAAACTTGCTCTACCCTCTGCAGCAATGGTATGTTTAGAGTATTGGGCCTTCGAGATTCTGGTGTTCATGGCAGGAGTGATGCCAAACGCAGAACAAAACACTTCCCTAATTGCAATCTGCACAAGGGTGTCAAATGAATTGGGAGCAGGCAATCCTGAGAAAGCCAAGAATGCAATGGCTGTGACTCTTAAGCTTTCTATACTTCTCGCTCTAGTTGTTGTTCTGGCTCTGGCATTTGGTCACAATTTATGGGCCGGCTCCTTCAGCAACAGCAGTGTAATAATAAAACTATTTGCTTCCATGACACCATTGCTTGCAGTTTCAATAATTGCTGATTCTGTGCAAGGCGTCTTATCAGGTGTGGCCAGAGGATGTGGGTGGCAGCACTTGGCTGTGTATGTAAACTTGGGAACATTCTATTTGGTTGGCATGACAATTGCAAGTGTTCTTGCATTTAAGTTGAAACTATATGCTAAGGGGTTGTGGATTGGTTTAATCTGCGGTCTTTCATGCCAAACCGCCCTACTTTTGGTGGTTACGCTACGCACAAAATGGACTCGACTGGATTTACCTCGTAATGCAGATGCAGTTTTGGCCTAA

Protein Analysis

461

Amino Acids

50.05

Weight (kDa)

6.74

Isoelectric Point (pI)

28.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 45 - 204 7.2e-33 MatE
MatE PF01554 298 - 407 4e-17 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000715)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23550 AT3G23560 AT3G23560
fragaria_vesca FvH4_1g15630 FvH4_1g15630 FvH4_3g19700 FvH4_3g19700 FvH4_3g19700 FvH4_3g19700 FvH4_3g19700 FvH4_3g19720 FvH4_3g20060 FvH4_3g20060 FvH4_3g20060 FvH4_3g20060 FvH4_5g31740 FvH4_7g19991
malus_domestica MD03G1230300.v1.1 MD11G1250700.v1.1 MD11G1251300.v1.1 MD11G1251400.v1.1 MD11G1251500.v1.1 MD12G1088700.v1.1
prunus_persica Prupe.4G177700_v2.0.a1 Prupe.4G177700_v2.0.a1 Prupe.4G177900_v2.0.a1 Prupe.4G178700_v2.0.a1 Prupe.4G178700_v2.0.a1 Prupe.4G178700_v2.0.a1 Prupe.4G178700_v2.0.a1 Prupe.4G178700_v2.0.a1 Prupe.4G178700_v2.0.a1 Prupe.4G178700_v2.0.a1 Prupe.4G178700_v2.0.a1 Prupe.4G178700_v2.0.a1 Prupe.4G178700_v2.0.a1 Prupe.4G178700_v2.0.a1 Prupe.4G178700_v2.0.a1 Prupe.4G178900_v2.0.a1 Prupe.4G178900_v2.0.a1 Prupe.4G178900_v2.0.a1 Prupe.4G206700_v2.0.a1
pyrus_communis pycom03g17780 pycom10g06980 pycom11g22170 pycom11g22270
rosa_chinensis RchiOBHm_Chr5g0033011 RchiOBHm_Chr5g0033061
rosa_laevigata RLG00000033443 RLG00000033445
rosa_multiflora Rmu_sc0000986.1_g000015
rosa_roxburghii Rroxscaffold_1G00046880 Rroxscaffold_1G00046890 Rroxscaffold_1G00046990 Rroxscaffold_1G00047000 Rroxscaffold_1G00047040
rosa_rugosa Rorug05G0135000 Rorug05G0135100 Rorug05G0135200 Rorug05G0135500 Rorug05G0135600 Rorug05G0135700 Rorug05G0135800
rosa_samantha Rh5AG227700 Rh5AG228200 Rh5BG229300 Rh5BG229400 Rh5CG256700 Rh5CG257200 Rh5DG233900 Rh5DG234100
rosa_wichuraiana Rw5G020860 Rw5G020880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 47
Acc16I TGCGCA 1 cut(s) 715
Acc36I ACCTGC 1 cut(s) 47
AccI GTMKAC 1 cut(s) 567
AciI CCGC 3 cut(s) 653, 1284, 1302
AclI AACGTT 2 cut(s) 150, 296
AclWI GGATC 3 cut(s) 160, 283, 574
AcoI YGGCCR 3 cut(s) 193, 696, 1146
AcsI RAATTY 3 cut(s) 235, 450, 479
AcuI CTGAAG 3 cut(s) 194, 521, 1030
AcyI GRCGYC 1 cut(s) 1131
AfaI GTAC 2 cut(s) 621, 704
AfiI CCNNNNNNNGG 3 cut(s) 49, 402, 1312
AflII CTTAAG 1 cut(s) 971
AgsI TTSAA 5 cut(s) 456, 672, 784, 1107, 1246
AjnI CCWGG 1 cut(s) 114
AleI CACNNNNGTG 1 cut(s) 908
AluBI AGCT 6 cut(s) 208, 214, 287, 650, 662, 976
AluI AGCT 6 cut(s) 208, 214, 287, 650, 662, 976
Alw21I GWGCWC 2 cut(s) 289, 652
Alw26I GTCTC 1 cut(s) 6
AlwI GGATC 3 cut(s) 160, 283, 574
Ama87I CYCGRG 1 cut(s) 490
AoxI GGCC 7 cut(s) 193, 243, 696, 828, 1035, 1146, 1377
ApeKI GCWGC 3 cut(s) 659, 803, 1165
ApoI RAATTY 3 cut(s) 235, 450, 479
Asp700I GAANNNNTTC 1 cut(s) 759
AspLEI GCGC 2 cut(s) 320, 716
AspS9I GGNCC 3 cut(s) 243, 828, 1035
AsuC2I CCSGG 2 cut(s) 491, 492
AsuHPI GGTGA 4 cut(s) 71, 215, 244, 589
AvaI CYCGRG 1 cut(s) 490
BalI TGGCCA 2 cut(s) 698, 1148
BanII GRGCYC 2 cut(s) 289, 652
BarI GAAGNNNNNNTAC 2 cut(s) 815, 847
Bbv12I GWGCWC 2 cut(s) 289, 652
BbvI GCAGC 3 cut(s) 671, 815, 1177
BccI CCATC 1 cut(s) 204
BceAI ACGGC 1 cut(s) 523
BciT130I CCWGG 1 cut(s) 116
BciVI GTATCC 1 cut(s) 403
BclI TGATCA 1 cut(s) 61
BcnI CCSGG 2 cut(s) 491, 492
BcoDI GTCTC 1 cut(s) 6
BfaI CTAG 2 cut(s) 290, 995
BfmI CTRYAG 1 cut(s) 801
BfrI CTTAAG 1 cut(s) 971
BfuAI ACCTGC 1 cut(s) 47
BfuI GTATCC 1 cut(s) 403
BisI GCNGC 3 cut(s) 660, 804, 1166
BlsI GCNGC 3 cut(s) 661, 805, 1167
Bme1390I CCNGG 3 cut(s) 116, 491, 492
BmeT110I CYCGRG 1 cut(s) 490
BmgT120I GGNCC 3 cut(s) 243, 828, 1035
BmiI GGNNCC 1 cut(s) 1042
BmrFI CCNGG 3 cut(s) 116, 491, 492
BmrI ACTGGG 1 cut(s) 578
BmsI GCATC 4 cut(s) 372, 675, 852, 1357
BmuI ACTGGG 1 cut(s) 578
Bpu10I CCTNAGC 1 cut(s) 1257
BpuMI CCSGG 2 cut(s) 491, 492
Bsa29I ATCGAT 1 cut(s) 683
BsaAI YACGTR 2 cut(s) 702, 735
BsaHI GRCGYC 1 cut(s) 1131
BsaJI CCNNGG 1 cut(s) 490
BsaXI ACNNNNNCTCC 1 cut(s) 33
Bsc4I CCNNNNNNNGG 3 cut(s) 49, 402, 1312
Bse118I RCCGGY 4 cut(s) 191, 218, 254, 1037
Bse1I ACTGG 3 cut(s) 567, 584, 1350
Bse3DI GCAATG 3 cut(s) 813, 963, 1091
BseBI CCWGG 1 cut(s) 116
BseCI ATCGAT 1 cut(s) 683
BseDI CCNNGG 1 cut(s) 490
BseGI GGATG 1 cut(s) 1160
BseLI CCNNNNNNNGG 3 cut(s) 49, 402, 1312
BseMI GCAATG 3 cut(s) 813, 963, 1091
BseMII CTCAG 1 cut(s) 930
BseNI ACTGG 3 cut(s) 567, 584, 1350
BseRI GAGGAG 1 cut(s) 116
BseXI GCAGC 3 cut(s) 671, 815, 1177
BseYI CCCAGC 1 cut(s) 337
BsgI GTGCAG 2 cut(s) 500, 886
BshFI GGCC 7 cut(s) 195, 245, 698, 830, 1037, 1148, 1379
BshVI ATCGAT 1 cut(s) 683
BsiHKAI GWGCWC 2 cut(s) 289, 652
BsiHKCI CYCGRG 1 cut(s) 490
BsiSI CCGG 5 cut(s) 192, 219, 255, 491, 1038
BsiWI CGTACG 1 cut(s) 702
BslI CCNNNNNNNGG 3 cut(s) 49, 402, 1312
BsmAI GTCTC 1 cut(s) 6
BsmI GAATGC 2 cut(s) 339, 958
BsnI GGCC 7 cut(s) 195, 245, 698, 830, 1037, 1148, 1379
BsoBI CYCGRG 1 cut(s) 490
Bsp1286I GDGCHC 2 cut(s) 289, 652
Bsp143I GATC 4 cut(s) 61, 165, 275, 579
BspACI CCGC 3 cut(s) 653, 1284, 1302
BspANI GGCC 7 cut(s) 195, 245, 698, 830, 1037, 1148, 1379
BspCNI CTCAG 1 cut(s) 931
BspDI ATCGAT 1 cut(s) 683
BspLI GGNNCC 1 cut(s) 1042
BspMAI CTGCAG 1 cut(s) 805
BspMI ACCTGC 1 cut(s) 47
BspPI GGATC 3 cut(s) 160, 283, 574
BspTI CTTAAG 1 cut(s) 971
BsrDI GCAATG 3 cut(s) 813, 963, 1091
BsrFI RCCGGY 4 cut(s) 191, 218, 254, 1037
BsrI ACTGG 3 cut(s) 567, 584, 1350
BssAI RCCGGY 4 cut(s) 191, 218, 254, 1037
BssECI CCNNGG 1 cut(s) 490
BssMI GATC 4 cut(s) 61, 165, 275, 579
BssNI GRCGYC 1 cut(s) 1131
Bst2UI CCWGG 1 cut(s) 116
Bst4CI ACNGT 1 cut(s) 250
Bst6I CTCTTC 1 cut(s) 63
BstACI GRCGYC 1 cut(s) 1131
BstAFI CTTAAG 1 cut(s) 971
BstAPI GCANNNNNTGC 1 cut(s) 230
BstBAI YACGTR 2 cut(s) 702, 735
BstC8I GCNNGC 6 cut(s) 193, 216, 657, 931, 1039, 1098
BstDEI CTNAG 3 cut(s) 97, 939, 1257
BstF5I GGATG 1 cut(s) 1160
BstHHI GCGC 2 cut(s) 320, 716
BstKTI GATC 4 cut(s) 64, 168, 278, 582
BstMAI GTCTC 1 cut(s) 6
BstMBI GATC 4 cut(s) 61, 165, 275, 579
BstMWI GCNNNNNNNGC 5 cut(s) 230, 656, 953, 1047, 1376
BstNI CCWGG 1 cut(s) 116
BstSCI CCNGG 3 cut(s) 114, 489, 490
BstSFI CTRYAG 1 cut(s) 801
BstV1I GCAGC 3 cut(s) 671, 815, 1177
Bsu15I ATCGAT 1 cut(s) 683
BsuI GTATCC 1 cut(s) 403
BsuRI GGCC 7 cut(s) 195, 245, 698, 830, 1037, 1148, 1379
BsuTUI ATCGAT 1 cut(s) 683
BtsCI GGATG 1 cut(s) 1160
BtsI GCAGTG 1 cut(s) 1063
BtsIMutI CAGTG 3 cut(s) 560, 591, 1063
BveI ACCTGC 1 cut(s) 47
Cac8I GCNNGC 6 cut(s) 193, 216, 657, 931, 1039, 1098
CfoI GCGC 2 cut(s) 320, 716
Cfr10I RCCGGY 4 cut(s) 191, 218, 254, 1037
Cfr13I GGNCC 3 cut(s) 243, 828, 1035
Cfr9I CCCGGG 1 cut(s) 490
ClaI ATCGAT 1 cut(s) 683
CseI GACGC 3 cut(s) 62, 550, 1120
CsiI ACCWGGT 1 cut(s) 114
Csp6I GTAC 2 cut(s) 620, 703
CviQI GTAC 2 cut(s) 620, 703
DdeI CTNAG 3 cut(s) 97, 939, 1257
DpnI GATC 4 cut(s) 63, 167, 277, 581
DpnII GATC 4 cut(s) 61, 165, 275, 579
EaeI YGGCCR 3 cut(s) 193, 696, 1146
Eam1104I CTCTTC 1 cut(s) 63
EarI CTCTTC 1 cut(s) 63
Ecl136II GAGCTC 2 cut(s) 287, 650
Eco24I GRGCYC 2 cut(s) 289, 652
Eco32I GATATC 1 cut(s) 681
Eco53kI GAGCTC 2 cut(s) 287, 650
Eco57I CTGAAG 3 cut(s) 194, 521, 1030
Eco88I CYCGRG 1 cut(s) 490
EcoICRI GAGCTC 2 cut(s) 287, 650
EcoRII CCWGG 1 cut(s) 114
EcoRV GATATC 1 cut(s) 681
EcoT38I GRGCYC 2 cut(s) 289, 652
FbaI TGATCA 1 cut(s) 61
FblI GTMKAC 1 cut(s) 567
Fnu4HI GCNGC 3 cut(s) 660, 804, 1166
FokI GGATG 1 cut(s) 1167
FriOI GRGCYC 2 cut(s) 289, 652
Fsp4HI GCNGC 3 cut(s) 660, 804, 1166
FspBI CTAG 2 cut(s) 290, 995
FspI TGCGCA 1 cut(s) 715
GlaI GCGC 2 cut(s) 319, 715
GluI GCNGC 3 cut(s) 660, 804, 1166
GsaI CCCAGC 1 cut(s) 341
HaeIII GGCC 7 cut(s) 195, 245, 698, 830, 1037, 1148, 1379
HapII CCGG 5 cut(s) 192, 219, 255, 491, 1038
HgaI GACGC 3 cut(s) 62, 550, 1120
HhaI GCGC 2 cut(s) 320, 716
Hin1I GRCGYC 1 cut(s) 1131
Hin6I GCGC 2 cut(s) 318, 714
HinP1I GCGC 2 cut(s) 318, 714
HindIII AAGCTT 1 cut(s) 974
HinfI GANTC 7 cut(s) 139, 312, 755, 838, 967, 1118, 1339
HpaII CCGG 5 cut(s) 192, 219, 255, 491, 1038
HphI GGTGA 4 cut(s) 71, 215, 244, 589
Hpy166II GTNNAC 2 cut(s) 568, 1186
Hpy188I TCNGA 3 cut(s) 144, 174, 540
Hpy188III TCNNGA 5 cut(s) 65, 290, 440, 835, 938
Hpy8I GTNNAC 2 cut(s) 568, 1186
HpyAV CCTTC 3 cut(s) 734, 841, 1054
HpyCH4III ACNGT 1 cut(s) 250
HpyCH4IV ACGT 5 cut(s) 150, 296, 701, 705, 734
HpyF10VI GCNNNNNNNGC 5 cut(s) 230, 656, 953, 1047, 1376
HpyF3I CTNAG 3 cut(s) 97, 939, 1257
HpySE526I ACGT 5 cut(s) 150, 296, 701, 705, 734
Hsp92I GRCGYC 1 cut(s) 1131
HspAI GCGC 2 cut(s) 318, 714
KroI GCCGGC 2 cut(s) 191, 1037
KroNI GCCGGC 2 cut(s) 193, 1039
Ksp22I TGATCA 1 cut(s) 61
Kzo9I GATC 4 cut(s) 61, 165, 275, 579
LmnI GCTCC 5 cut(s) 284, 647, 655, 926, 1046
Lsp1109I GCAGC 3 cut(s) 671, 815, 1177
LweI GCATC 4 cut(s) 372, 675, 852, 1357
MabI ACCWGGT 1 cut(s) 114
MaeI CTAG 2 cut(s) 290, 995
MaeII ACGT 5 cut(s) 150, 296, 701, 705, 734
MaeIII GTNAC 5 cut(s) 250, 730, 964, 1022, 1318
MalI GATC 4 cut(s) 63, 167, 277, 581
MboI GATC 4 cut(s) 61, 165, 275, 579
MboII GAAGA 3 cut(s) 80, 527, 584
MfeI CAATTG 1 cut(s) 1218
MhlI GDGCHC 2 cut(s) 289, 652
MlsI TGGCCA 2 cut(s) 698, 1148
MluNI TGGCCA 2 cut(s) 698, 1148
MlyI GAGTC 2 cut(s) 961, 1333
MmeI TCCRAC 4 cut(s) 30, 253, 580, 801
Mox20I TGGCCA 2 cut(s) 698, 1148
MroNI GCCGGC 2 cut(s) 191, 1037
MroXI GAANNNNTTC 1 cut(s) 759
MscI TGGCCA 2 cut(s) 698, 1148
MseI TTAA 4 cut(s) 431, 972, 1239, 1277
MslI CAYNNNNRTG 2 cut(s) 201, 908
Msp20I TGGCCA 2 cut(s) 698, 1148
MspA1I CMGCKG 1 cut(s) 655
MspCI CTTAAG 1 cut(s) 971
MspI CCGG 5 cut(s) 192, 219, 255, 491, 1038
MspR9I CCNGG 3 cut(s) 116, 491, 492
MunI CAATTG 1 cut(s) 1218
Mva1269I GAATGC 2 cut(s) 339, 958
MvaI CCWGG 1 cut(s) 116
MwoI GCNNNNNNNGC 5 cut(s) 230, 656, 953, 1047, 1376
NaeI GCCGGC 2 cut(s) 193, 1039
NciI CCSGG 2 cut(s) 491, 492
NdeII GATC 4 cut(s) 61, 165, 275, 579
NgoMIV GCCGGC 2 cut(s) 191, 1037
NlaIV GGNNCC 1 cut(s) 1042
NmuCI GTSAC 3 cut(s) 250, 964, 1022
NsbI TGCGCA 1 cut(s) 715
OliI CACNNNNGTG 1 cut(s) 908
PaqCI CACCTGC 1 cut(s) 47
PcsI WCGNNNNNNNCGW 1 cut(s) 393
PctI GAATGC 2 cut(s) 339, 958
PdiI GCCGGC 2 cut(s) 193, 1039
PdmI GAANNNNTTC 1 cut(s) 759
PfeI GAWTC 5 cut(s) 139, 312, 755, 838, 1118
Pfl23II CGTACG 1 cut(s) 702
PkrI GCNGC 3 cut(s) 661, 805, 1167
PleI GAGTC 2 cut(s) 961, 1333
PpsI GAGTC 2 cut(s) 961, 1333
Ppu21I YACGTR 2 cut(s) 702, 735
Psp124BI GAGCTC 2 cut(s) 289, 652
Psp1406I AACGTT 2 cut(s) 150, 296
Psp6I CCWGG 1 cut(s) 114
PspFI CCCAGC 1 cut(s) 337
PspGI CCWGG 1 cut(s) 114
PspLI CGTACG 1 cut(s) 702
PspN4I GGNNCC 1 cut(s) 1042
PspPI GGNCC 3 cut(s) 243, 828, 1035
PstI CTGCAG 1 cut(s) 805
RsaI GTAC 2 cut(s) 621, 704
RsaNI GTAC 2 cut(s) 620, 703
RseI CAYNNNNRTG 2 cut(s) 201, 908
SacI GAGCTC 2 cut(s) 289, 652
SaqAI TTAA 4 cut(s) 431, 972, 1239, 1277
SatI GCNGC 3 cut(s) 660, 804, 1166
Sau3AI GATC 4 cut(s) 61, 165, 275, 579
Sau96I GGNCC 3 cut(s) 243, 828, 1035
SchI GAGTC 2 cut(s) 961, 1333
ScrFI CCNGG 3 cut(s) 116, 491, 492
SduI GDGCHC 2 cut(s) 289, 652
SexAI ACCWGGT 1 cut(s) 114
SfaNI GCATC 4 cut(s) 372, 675, 852, 1357
SfcI CTRYAG 1 cut(s) 801
SmaI CCCGGG 1 cut(s) 492
SmiMI CAYNNNNRTG 2 cut(s) 201, 908
SmlI CTYRAG 1 cut(s) 971
SmoI CTYRAG 1 cut(s) 971
SsiI CCGC 3 cut(s) 653, 1284, 1302
SspMI CTAG 2 cut(s) 290, 995
SstI GAGCTC 2 cut(s) 289, 652
StyD4I CCNGG 3 cut(s) 114, 489, 490
TaaI ACNGT 1 cut(s) 250
TaiI ACGT 5 cut(s) 153, 299, 704, 708, 737
TaqI TCGA 4 cut(s) 665, 683, 834, 1342
TfiI GAWTC 5 cut(s) 139, 312, 755, 838, 1118
Tru1I TTAA 4 cut(s) 431, 972, 1239, 1277
Tru9I TTAA 4 cut(s) 431, 972, 1239, 1277
TscAI CASTG 3 cut(s) 567, 591, 1063
TseFI GTSAC 3 cut(s) 250, 964, 1022
TseI GCWGC 3 cut(s) 659, 803, 1165
Tsp45I GTSAC 3 cut(s) 250, 964, 1022
TspGWI ACGGA 2 cut(s) 24, 385
TspMI CCCGGG 1 cut(s) 490
TspRI CASTG 3 cut(s) 567, 591, 1063
Vha464I CTTAAG 1 cut(s) 971
XapI RAATTY 3 cut(s) 235, 450, 479
XbaI TCTAGA 1 cut(s) 289
XcmI CCANNNNNNNNNTGG 1 cut(s) 955
XmaI CCCGGG 1 cut(s) 490
XmiI GTMKAC 1 cut(s) 567
XmnI GAANNNNTTC 1 cut(s) 759
XspI CTAG 2 cut(s) 290, 995
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.