pycom14g20500

Belongs to the argonaute family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0001348
Physical Location & Seq
Forward (+)
21549374 .. 21555276
5903 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3243 bp
ATGGTAAGGAAGAGGAGAACTGAACTCCCTAGTGGGGAAAGTTCCGAGTCTCATGAACCTGCTGGGGGTAGTGGTCGTGGTTCCCAGCGTGCTCCTGAAAGGACTCCTCCACAACAACAGGGTGGAGGAAACTACCGAGGTGGTCGTGGACCCGCCCCCCAGGGAGGTCGTGGAGTCTATGGAGGGGGTCGTGGTGGCCGCGGAGTGCCACAACAGCAGCAATATGGCGCGCCCCAAGAATACCAACAACAGCAGTATGGTGGGCCACAGGAATATCAAGGCAGAGGCAGGGGAGGACCGACTCAGCAAGGAGGTCATGGAGGTTATGGAGGTGGCCGTGGTGGCATTGGCCGTGGAGGACCACCTTCTCCTGGTGGTCCAACCAGACCTCAGTTCCCCGAGCTGCACCAAGCAACCCCAGTTCCTTACCAAGAGGCCACCCCCCAAGTTCCTTATCAAGGGGTTACCTCTCCGCCTGTATATGGTGGAAGTTCATCATCTTCCCAGCCACCTGAGCCTTCAGAAGTAGCGGAGCAGTTTGCTGACCTTACCGTCCAGCAAGAGAGTGCACCTAGCCAGGCTATTCAACCTGTGGCGCCCTCAAGTAAGTCAGTCAGGTTCCCTCTTCGCCCGGGTAAGGGTAGCACTGGCAGAAGGTGTACAGTCAAGGCAAACCATTTCTTTGCAGAATTACCCGACAAAGACCTCCACCAGTATGATGTTACTATTACACCAGAGGTCACATCAAGGGGTGTTAATCGTGCTGTGATGGAGCAGCTGGTGAAACAGTACAGGGAATCCCATCTTGGAAAACGTCTACCAGCTTATGATGGACGAAAGAGCCTGTATACTGCCGGCCCACTTCCTTTCCTTTCAAAGGAGTTTAAAATCACTCTTATTGATGATGATGATCGATCAGGTGGACAAAGGAGAGAGAGGGAATTTAGGGTTGTCATAAAATTTGCAGCACGTGCTGACCTACACCATTTAGGTCTCTTTTTGCAAGGGAGGCAAGCTGATGCACCTCAAGAGGCACTTCAGGTTTTGGACATTGTTCTGCGCGAATTGCCTACTTCTAGGTACTGTCCTGTGGGCCGTTCATTTTATTCCCCTGATTTGGGTAGAAGACAGTCACTTGGTGATGGTTTGGAAAGTTGGCGTGGTTTTTATCAGAGTATTCGTCCAACTCAGATGGGGCTGTCTCTGAATATTGACATGTCCTCTACTGCTTTTATTGAGCCGTTGCCCGTTATTGAGTTCGTAACAGAGCTACTGAATCGGGATGTTACACATAGGCCACTTTCTGATTCTGATCGTGTAAAGATTAAAAAGGCTCTTCGTGGAGTCAAGGTTGAGGTCACACACCGAGGAAATATGCGCAGAAAGTACCGTATTTCTGGTTTAACGTCACAGGCAACAAGGGAACTGACTTTCCCTGTTGATGAAAAAAGAACTATGAAATCTGTTGTTGAGTACTTCTATGAAACTTATGGTTTCGTCATTCAACATACACAATGGCCTTGCCTTCAAGTTGGAAATCAACAGCGGCCGAATTATTTGCCCATGGAGGTTTGTAAAATTGTCGAAGGTCAAAGGTATTCCAAGAGATTGAACGAGAAACAGATTACTGCTTTGCTTAAGGTCACCTGTCAGCGCCCTCATGACAGGGAACAGGATATTATTCGGACGGTTCGTCATAATGCTTACCACGAAGATCCTTATGCCAAGGAGTTTGGAATCAAAATTAGTGAGAATCTGGCTCAGGTTGAAGCTCGTATTCTTCCTGCACCCTGGCTGAAATATCATGATACTGGAAGGGAAAAGGATTGCCTGCCCCAAGTTGGTCAGTGGAATATGATGAATAAGAAAATGGTCAATGGAGGAAGAGTAAACAATTGGATTTGCATTAACTTTTCACGAAATGTTCAAGACAACGTGGCCCGTTCCTTCTGTAATGAACTTGCTCAAATGTGTTACATTTCTGGCATGGCGTTTAATCCAGAATCGGTACTTCCACCAATCAGTGCTCGACCTGATCAGGCAGAGAAGGCCTTAAAAACTAGGTACCATGATGCAATGACCAAACTCCGACCCCAGAACAAGGAGCTTGACCTGCTTGTCGTTATTTTACCAGATAATAATGGCAATCTTTATGGTGACTTGAAACGAATTTGTGAGACGGATCTTGGCCTGGTATCCCAGTGCTGTTTGACGAAACATGTTTTCAGAATGAGTAAACAATACTTGGCAAATGTTGCTTTGAAGATAAATGTGAAGGTTGGCGGAAGGAACACAGTGCTTGTTGATGCACTGTCAAGACGTATTCCTTTAGTCAGTGACCGTCCTACTATAATTTTTGGTGCTGATGTTACCCATCCTCATCCCGGGGAGGATTCAAGCCCATCAATAGCAGCTGTTGTGGCCTCACAAGATTGGCCAGAAATCACCAAGTACGCTGGCTTGGTCTGTGCGCAAGCTCATCGACAGGAGCTTATCCAAGACCTCTTCAAAACTTGGCAGGATCCTCAGAGGGGCACTGTAACTGGTGGAATGATAAAGGAACTGCTAATTTCGTTCCGTAGAGCAACAGGACAGAAGCCACAGCGCATCATATTTTACAGGGACGGTGTCAGCGAGGGCCAGTTCTACCAAGTTCTACTATACGAGCTTGATGCGATTCGTAAGGCTTGTGCTTCCTTGGAGCCAAACTATCAGCCACCTGTGACTTTTGTTGTGGTTCAGAAACGTCACCACACAAGGCTGTTTGCCAACAATCATAATGACCGTAGTGCAGTTGACAGGAGTGGAAATATCTTACCTGGCACGGTTGTGGACTCCAAAATCTGTCATCCAACGGAGTTTGACTTCTACCTCTGCAGCCATGCTGGAATTCAGGGTACAAGCCGTCCGGCTCATTACCACGTGCTGTGGGATGAAAACAAGTTTACAGCTGATGAACTGCAGTCACTCACAAACAATCTTTGCTATACGTACGCAAGATGCACGCGTTCGGTTTCCATTGTTCCTCCGGCTTACTACGCCCATCTTGCTGCATTCCGAGCTCGGTTTTACTTGGAACCAGAGACATCAGACAGCGGATCAATGACCAGTGGTGCTCCTGGACGCGGGGGCATGGGTGGGGGTATGGGTCCGCGGAGCACAAGAGCACCAGGTCCAAATGCTGCAGTGAGGCCCTTGCCTGCCCTGAAAGAGAATGTCAAGAGGGTTATGTTTTACTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ArgoL1 ArgoL2 ArgoMid ArgoN Gly-rich_Ago1 PAZ Piwi

Protein Analysis

1081

Amino Acids

120.36

Weight (kDa)

9.4

Isoelectric Point (pI)

54.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gly-rich_Ago1 PF12764 87 - 198 1.3e-17 Glycine-rich region of argonaut
ArgoN PF16486 218 - 353 1.5e-38 N-terminal domain of argonaute
ArgoL1 PF08699 363 - 413 7.2e-22 Argonaute linker 1 domain
PAZ PF02170 420 - 545 4.2e-30 PAZ domain
ArgoL2 PF16488 555 - 601 3.3e-16 Argonaute linker 2 domain
ArgoMid PF16487 612 - 686 5.8e-09 Mid domain of argonaute
Piwi PF02171 706 - 1024 2.2e-116 Piwi domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 551, 2117
Acc16I TGCGCA 2 cut(s) 1379, 2472
Acc36I ACCTGC 2 cut(s) 67, 2121
Acc65I GGTACC 1 cut(s) 2064
AccB1I GGYRCC 2 cut(s) 595, 2064
AccI GTMKAC 2 cut(s) 817, 848
AccII CGCG 6 cut(s) 201, 230, 1062, 3007, 3126, 3154
AclWI GGATC 5 cut(s) 1709, 2190, 2516, 2529, 3106
AcoI YGGCCR 5 cut(s) 196, 334, 349, 1547, 2435
AcsI RAATTY 4 cut(s) 941, 959, 2169, 2889
AcuI CTGAAG 2 cut(s) 504, 1022
AcvI CACGTG 2 cut(s) 971, 2923
AcyI GRCGYC 1 cut(s) 596
AdeI CACNNNGTG 1 cut(s) 1139
AflII CTTAAG 1 cut(s) 1637
AflIII ACRYGT 3 cut(s) 1215, 2218, 3005
AhdI GACNNNNNGTC 1 cut(s) 1692
AjnI CCWGG 8 cut(s) 159, 370, 576, 1790, 2190, 2818, 3118, 3169
AjuI GAANNNNNNNTTGG 2 cut(s) 789, 821
AleI CACNNNNGTG 1 cut(s) 2828
AloI GAACNNNNNNTCC 2 cut(s) 1416, 1448
Alw21I GWGCWC 7 cut(s) 94, 571, 2029, 3064, 3118, 3161, 3169
Alw26I GTCTC 5 cut(s) 54, 998, 1206, 2171, 3077
Alw44I GTGCAC 1 cut(s) 567
AlwI GGATC 5 cut(s) 1709, 2190, 2516, 2529, 3106
Ama87I CYCGRG 3 cut(s) 398, 631, 2384
ApaLI GTGCAC 1 cut(s) 567
ApeKI GCWGC 8 cut(s) 217, 403, 775, 965, 2411, 2877, 3050, 3182
ApoI RAATTY 4 cut(s) 941, 959, 2169, 2889
AscI GGCGCGCC 1 cut(s) 228
Asp718I GGTACC 1 cut(s) 2064
AspLEI GCGC 8 cut(s) 230, 232, 598, 1062, 1380, 1656, 2473, 2607
AsuC2I CCSGG 4 cut(s) 632, 633, 2385, 2386
AsuHPI GGTGA 6 cut(s) 793, 1151, 1636, 2168, 2437, 2741
AvaI CYCGRG 3 cut(s) 398, 631, 2384
AvaII GGWCC 6 cut(s) 149, 296, 359, 377, 3149, 3173
BaeGI GKGCMC 2 cut(s) 571, 2537
BalI TGGCCA 1 cut(s) 2437
BamHI GGATCC 1 cut(s) 2521
BanI GGYRCC 2 cut(s) 595, 2064
BanII GRGCYC 1 cut(s) 3064
BbrPI CACGTG 2 cut(s) 971, 2923
BbsI GAAGAC 1 cut(s) 1132
Bbv12I GWGCWC 7 cut(s) 94, 571, 2029, 3064, 3118, 3161, 3169
BbvI GCAGC 8 cut(s) 229, 390, 787, 977, 2423, 2889, 3037, 3169
BccI CCATC 8 cut(s) 763, 810, 824, 1136, 1186, 2382, 2410, 3051
BceAI ACGGC 5 cut(s) 321, 336, 1080, 1225, 2889
BcgI CGANNNNNNTGC 6 cut(s) 1540, 1574, 2462, 2496, 2654, 2688
BciT130I CCWGG 8 cut(s) 161, 372, 578, 1792, 2192, 2820, 3120, 3171
BciVI GTATCC 1 cut(s) 2206
BclI TGATCA 1 cut(s) 2035
BcnI CCSGG 4 cut(s) 632, 633, 2385, 2386
BcoDI GTCTC 5 cut(s) 54, 998, 1206, 2171, 3077
BfaI CTAG 4 cut(s) 30, 573, 1077, 2061
BfmI CTRYAG 3 cut(s) 2875, 2960, 3183
BfoI RGCGCY 2 cut(s) 599, 1657
BfrI CTTAAG 1 cut(s) 1637
BfuAI ACCTGC 2 cut(s) 67, 2121
BfuI GTATCC 1 cut(s) 2206
BglI GCCNNNNNGGC 1 cut(s) 342
BmcAI AGTACT 1 cut(s) 1475
Bme18I GGWCC 6 cut(s) 149, 296, 359, 377, 3149, 3173
BmeRI GACNNNNNGTC 1 cut(s) 1692
BmeT110I CYCGRG 3 cut(s) 398, 631, 2384
BmiI GGNNCC 9 cut(s) 82, 151, 597, 620, 2066, 2523, 2703, 3078, 3150
BmrI ACTGGG 2 cut(s) 413, 2194
BmsI GCATC 6 cut(s) 1009, 2062, 2296, 2616, 2662, 2990
BmuI ACTGGG 2 cut(s) 413, 2194
BpiI GAAGAC 1 cut(s) 1132
Bpu10I CCTNAGC 2 cut(s) 513, 1761
BpuEI CTTGAG 2 cut(s) 586, 1011
BpuMI CCSGG 4 cut(s) 632, 633, 2385, 2386
Bsa29I ATCGAT 1 cut(s) 913
BsaAI YACGTR 3 cut(s) 971, 2923, 2991
BsaBI GATNNNNATC 2 cut(s) 909, 1311
BsaHI GRCGYC 1 cut(s) 596
BsaI GGTCTC 1 cut(s) 998
BsaXI ACNNNNNCTCC 4 cut(s) 1335, 1365, 1872, 1902
Bse118I RCCGGY 1 cut(s) 854
Bse1I ACTGG 8 cut(s) 419, 652, 712, 1816, 2200, 2548, 2641, 3108
Bse3DI GCAATG 1 cut(s) 2082
Bse8I GATNNNNATC 2 cut(s) 909, 1311
BseBI CCWGG 8 cut(s) 161, 372, 578, 1792, 2192, 2820, 3120, 3171
BseCI ATCGAT 1 cut(s) 913
BseGI GGATG 5 cut(s) 1288, 2374, 2380, 2848, 2938
BseJI GATNNNNATC 2 cut(s) 909, 1311
BseMI GCAATG 1 cut(s) 2082
BseMII CTCAG 6 cut(s) 317, 404, 504, 1202, 1775, 2540
BseNI ACTGG 8 cut(s) 419, 652, 712, 1816, 2200, 2548, 2641, 3108
BsePI GCGCGC 1 cut(s) 228
BseRI GAGGAG 2 cut(s) 28, 96
BseSI GKGCMC 2 cut(s) 571, 2537
BseX3I CGGCCG 1 cut(s) 1547
BseXI GCAGC 8 cut(s) 229, 390, 787, 977, 2423, 2889, 3037, 3169
BseYI CCCAGC 3 cut(s) 62, 84, 504
BsgI GTGCAG 3 cut(s) 389, 1770, 2811
Bsh1236I CGCG 6 cut(s) 201, 230, 1062, 3007, 3126, 3154
Bsh1285I CGRYCG 1 cut(s) 1550
BshNI GGYRCC 2 cut(s) 595, 2064
BshVI ATCGAT 1 cut(s) 913
BsiEI CGRYCG 1 cut(s) 1550
BsiHKAI GWGCWC 7 cut(s) 94, 571, 2029, 3064, 3118, 3161, 3169
BsiHKCI CYCGRG 3 cut(s) 398, 631, 2384
BsiSI CCGG 5 cut(s) 632, 855, 2385, 2909, 3029
BsiWI CGTACG 1 cut(s) 2991
BslFI GGGAC 1 cut(s) 2636
BsmAI GTCTC 5 cut(s) 54, 998, 1206, 2171, 3077
BsmBI CGTCTC 1 cut(s) 2171
BsmFI GGGAC 1 cut(s) 2636
BsmI GAATGC 1 cut(s) 3053
Bso31I GGTCTC 1 cut(s) 998
BsoBI CYCGRG 3 cut(s) 398, 631, 2384
Bsp1286I GDGCHC 8 cut(s) 94, 571, 2029, 2537, 3064, 3118, 3161, 3169
Bsp1407I TGTACA 1 cut(s) 659
Bsp143I GATC 8 cut(s) 910, 914, 1312, 1714, 2035, 2182, 2521, 3098
Bsp19I CCATGG 1 cut(s) 1563
BspCNI CTCAG 6 cut(s) 316, 403, 505, 1201, 1774, 2539
BspDI ATCGAT 1 cut(s) 913
BspFNI CGCG 6 cut(s) 201, 230, 1062, 3007, 3126, 3154
BspHI TCATGA 3 cut(s) 52, 1660, 1804
BspLI GGNNCC 9 cut(s) 82, 151, 597, 620, 2066, 2523, 2703, 3078, 3150
BspMAI CTGCAG 3 cut(s) 2879, 2964, 3187
BspMI ACCTGC 2 cut(s) 67, 2121
BspPI GGATC 5 cut(s) 1709, 2190, 2516, 2529, 3106
BspQI GCTCTTC 1 cut(s) 1341
BspT107I GGYRCC 2 cut(s) 595, 2064
BspTI CTTAAG 1 cut(s) 1637
BspTNI GGTCTC 1 cut(s) 998
BsrDI GCAATG 1 cut(s) 2082
BsrFI RCCGGY 1 cut(s) 854
BsrGI TGTACA 1 cut(s) 659
BsrI ACTGG 8 cut(s) 419, 652, 712, 1816, 2200, 2548, 2641, 3108
BssAI RCCGGY 1 cut(s) 854
BssHII GCGCGC 1 cut(s) 228
BssMI GATC 8 cut(s) 910, 914, 1312, 1714, 2035, 2182, 2521, 3098
BssNAI GTATAC 1 cut(s) 849
BssNI GRCGYC 1 cut(s) 596
BssT1I CCWWGG 3 cut(s) 1563, 1725, 2697
Bst1107I GTATAC 1 cut(s) 849
Bst2UI CCWGG 8 cut(s) 161, 372, 578, 1792, 2192, 2820, 3120, 3171
Bst6I CTCTTC 5 cut(s) 5, 630, 1341, 1879, 2510
BstACI GRCGYC 1 cut(s) 596
BstAFI CTTAAG 1 cut(s) 1637
BstAPI GCANNNNNTGC 2 cut(s) 971, 2255
BstAUI TGTACA 1 cut(s) 659
BstBAI YACGTR 3 cut(s) 971, 2923, 2991
BstC8I GCNNGC 9 cut(s) 90, 230, 856, 1014, 1832, 2458, 2475, 3005, 3201
BstDEI CTNAG 6 cut(s) 303, 390, 513, 1188, 1761, 2526
BstDSI CCRYGG 5 cut(s) 199, 337, 352, 1563, 3152
BstEII GGTNACC 2 cut(s) 463, 1642
BstENI CCTNNNNNAGG 2 cut(s) 456, 875
BstF5I GGATG 5 cut(s) 1288, 2374, 2380, 2848, 2938
BstFNI CGCG 6 cut(s) 201, 230, 1062, 3007, 3126, 3154
BstH2I RGCGCY 2 cut(s) 599, 1657
BstHHI GCGC 8 cut(s) 230, 232, 598, 1062, 1380, 1656, 2473, 2607
BstKTI GATC 8 cut(s) 913, 917, 1315, 1717, 2038, 2185, 2524, 3101
BstMAI GTCTC 5 cut(s) 54, 998, 1206, 2171, 3077
BstMBI GATC 8 cut(s) 910, 914, 1312, 1714, 2035, 2182, 2521, 3098
BstMCI CGRYCG 1 cut(s) 1550
BstNI CCWGG 8 cut(s) 161, 372, 578, 1792, 2192, 2820, 3120, 3171
BstNSI RCATGY 2 cut(s) 1219, 2222
BstPI GGTNACC 2 cut(s) 463, 1642
BstSFI CTRYAG 3 cut(s) 2875, 2960, 3183
BstSLI GKGCMC 2 cut(s) 571, 2537
BstSNI TACGTA 1 cut(s) 2991
BstUI CGCG 6 cut(s) 201, 230, 1062, 3007, 3126, 3154
BstV1I GCAGC 8 cut(s) 229, 390, 787, 977, 2423, 2889, 3037, 3169
BstV2I GAAGAC 1 cut(s) 1132
BstX2I RGATCY 3 cut(s) 1714, 2182, 2521
BstYI RGATCY 3 cut(s) 1714, 2182, 2521
BstZ17I GTATAC 1 cut(s) 849
BstZI CGGCCG 1 cut(s) 1547
Bsu15I ATCGAT 1 cut(s) 913
BsuI GTATCC 1 cut(s) 2206
BsuTUI ATCGAT 1 cut(s) 913
BtgI CCRYGG 5 cut(s) 199, 337, 352, 1563, 3152
BtsCI GGATG 5 cut(s) 1288, 2374, 2380, 2848, 2938
BtsI GCAGTG 1 cut(s) 3192
BveI ACCTGC 2 cut(s) 67, 2121
Cac8I GCNNGC 9 cut(s) 90, 230, 856, 1014, 1832, 2458, 2475, 3005, 3201
CciI TCATGA 3 cut(s) 52, 1660, 1804
CfoI GCGC 8 cut(s) 230, 232, 598, 1062, 1380, 1656, 2473, 2607
Cfr10I RCCGGY 1 cut(s) 854
Cfr42I CCGCGG 2 cut(s) 202, 3155
Cfr9I CCCGGG 2 cut(s) 631, 2384
ClaI ATCGAT 1 cut(s) 913
CseI GACGC 1 cut(s) 3132
CsiI ACCWGGT 1 cut(s) 3169
DdeI CTNAG 6 cut(s) 303, 390, 513, 1188, 1761, 2526
DinI GGCGCC 1 cut(s) 597
DpnI GATC 8 cut(s) 912, 916, 1314, 1716, 2037, 2184, 2523, 3100
DpnII GATC 8 cut(s) 910, 914, 1312, 1714, 2035, 2182, 2521, 3098
DraI TTTAAA 1 cut(s) 886
DraIII CACNNNGTG 1 cut(s) 1139
DrdI GACNNNNNNGTC 2 cut(s) 551, 2117
DriI GACNNNNNGTC 1 cut(s) 1692
DseDI GACNNNNNNGTC 2 cut(s) 551, 2117
EaeI YGGCCR 5 cut(s) 196, 334, 349, 1547, 2435
EagI CGGCCG 1 cut(s) 1547
Eam1104I CTCTTC 5 cut(s) 5, 630, 1341, 1879, 2510
Eam1105I GACNNNNNGTC 1 cut(s) 1692
EarI CTCTTC 5 cut(s) 5, 630, 1341, 1879, 2510
EciI GGCGGA 2 cut(s) 462, 2298
Ecl136II GAGCTC 1 cut(s) 3062
EclXI CGGCCG 1 cut(s) 1547
Eco105I TACGTA 1 cut(s) 2991
Eco130I CCWWGG 3 cut(s) 1563, 1725, 2697
Eco147I AGGCCT 1 cut(s) 2051
Eco24I GRGCYC 1 cut(s) 3064
Eco31I GGTCTC 1 cut(s) 998
Eco47I GGWCC 6 cut(s) 149, 296, 359, 377, 3149, 3173
Eco52I CGGCCG 1 cut(s) 1547
Eco53kI GAGCTC 1 cut(s) 3062
Eco57I CTGAAG 2 cut(s) 504, 1022
Eco72I CACGTG 2 cut(s) 971, 2923
Eco88I CYCGRG 3 cut(s) 398, 631, 2384
Eco91I GGTNACC 2 cut(s) 463, 1642
EcoICRI GAGCTC 1 cut(s) 3062
EcoNI CCTNNNNNAGG 2 cut(s) 456, 875
EcoO109I RGGNCCY 1 cut(s) 3192
EcoO65I GGTNACC 2 cut(s) 463, 1642
EcoRI GAATTC 1 cut(s) 2889
EcoRII CCWGG 8 cut(s) 159, 370, 576, 1790, 2190, 2818, 3118, 3169
EcoT14I CCWWGG 3 cut(s) 1563, 1725, 2697
EcoT38I GRGCYC 1 cut(s) 3064
EgeI GGCGCC 1 cut(s) 597
EheI GGCGCC 1 cut(s) 597
ErhI CCWWGG 3 cut(s) 1563, 1725, 2697
Esp3I CGTCTC 1 cut(s) 2171
FalI AAGNNNNNCTT 2 cut(s) 1020, 1052
FaqI GGGAC 1 cut(s) 2636
FauI CCCGC 2 cut(s) 160, 3119
FbaI TGATCA 1 cut(s) 2035
FblI GTMKAC 2 cut(s) 817, 848
FokI GGATG 5 cut(s) 1295, 2361, 2367, 2835, 2945
FriOI GRGCYC 1 cut(s) 3064
FspBI CTAG 4 cut(s) 30, 573, 1077, 2061
FspI TGCGCA 2 cut(s) 1379, 2472
GlaI GCGC 8 cut(s) 229, 231, 597, 1061, 1379, 1655, 2472, 2606
GsaI CCCAGC 3 cut(s) 66, 88, 508
HaeII RGCGCY 2 cut(s) 599, 1657
HapII CCGG 5 cut(s) 632, 855, 2385, 2909, 3029
HgaI GACGC 1 cut(s) 3132
HhaI GCGC 8 cut(s) 230, 232, 598, 1062, 1380, 1656, 2473, 2607
Hin1I GRCGYC 1 cut(s) 596
Hin6I GCGC 8 cut(s) 228, 230, 596, 1060, 1378, 1654, 2471, 2605
HinP1I GCGC 8 cut(s) 228, 230, 596, 1060, 1378, 1654, 2471, 2605
HincII GTYRAC 1 cut(s) 2797
HindII GTYRAC 1 cut(s) 2797
HpaII CCGG 5 cut(s) 632, 855, 2385, 2909, 3029
HphI GGTGA 6 cut(s) 793, 1151, 1636, 2168, 2437, 2741
HpyCH4IV ACGT 8 cut(s) 814, 970, 1406, 1935, 2320, 2746, 2922, 2990
HpyF3I CTNAG 6 cut(s) 303, 390, 513, 1188, 1761, 2526
HpySE526I ACGT 8 cut(s) 814, 970, 1406, 1935, 2320, 2746, 2922, 2990
Hsp92I GRCGYC 1 cut(s) 596
HspAI GCGC 8 cut(s) 228, 230, 596, 1060, 1378, 1654, 2471, 2605
KasI GGCGCC 1 cut(s) 595
KpnI GGTACC 1 cut(s) 2068
KroI GCCGGC 1 cut(s) 854
KroNI GCCGGC 1 cut(s) 856
Ksp22I TGATCA 1 cut(s) 2035
KspI CCGCGG 2 cut(s) 202, 3155
Kzo9I GATC 8 cut(s) 910, 914, 1312, 1714, 2035, 2182, 2521, 3098
LguI GCTCTTC 1 cut(s) 1341
LmnI GCTCC 8 cut(s) 97, 532, 772, 2104, 2488, 2701, 3121, 3156
Lsp1109I GCAGC 8 cut(s) 229, 390, 787, 977, 2423, 2889, 3037, 3169
LweI GCATC 6 cut(s) 1009, 2062, 2296, 2616, 2662, 2990
MabI ACCWGGT 1 cut(s) 3169
MaeI CTAG 4 cut(s) 30, 573, 1077, 2061
MaeII ACGT 8 cut(s) 814, 970, 1406, 1935, 2320, 2746, 2922, 2990
MalI GATC 8 cut(s) 912, 916, 1314, 1716, 2037, 2184, 2523, 3100
MboI GATC 8 cut(s) 910, 914, 1312, 1714, 2035, 2182, 2521, 3098
MfeI CAATTG 1 cut(s) 1894
MflI RGATCY 3 cut(s) 1714, 2182, 2521
MhlI GDGCHC 8 cut(s) 94, 571, 2029, 2537, 3064, 3118, 3161, 3169
MlsI TGGCCA 1 cut(s) 2437
MluI ACGCGT 1 cut(s) 3005
MluNI TGGCCA 1 cut(s) 2437
Mly113I GGCGCC 1 cut(s) 596
MlyI GAGTC 6 cut(s) 56, 97, 183, 295, 1353, 2828
MmeI TCCRAC 5 cut(s) 404, 1208, 1513, 2113, 2876
Mox20I TGGCCA 1 cut(s) 2437
MroNI GCCGGC 1 cut(s) 854
MscI TGGCCA 1 cut(s) 2437
MseI TTAA 8 cut(s) 756, 885, 1326, 1403, 1638, 1908, 1995, 2054
MslI CAYNNNNRTG 2 cut(s) 714, 2828
Msp20I TGGCCA 1 cut(s) 2437
MspA1I CMGCKG 7 cut(s) 201, 778, 1546, 2414, 2951, 3096, 3154
MspCI CTTAAG 1 cut(s) 1637
MspI CCGG 5 cut(s) 632, 855, 2385, 2909, 3029
MunI CAATTG 1 cut(s) 1894
Mva1269I GAATGC 1 cut(s) 3053
MvaI CCWGG 8 cut(s) 161, 372, 578, 1792, 2192, 2820, 3120, 3171
MvnI CGCG 6 cut(s) 201, 230, 1062, 3007, 3126, 3154
NaeI GCCGGC 1 cut(s) 856
NarI GGCGCC 1 cut(s) 596
NciI CCSGG 4 cut(s) 632, 633, 2385, 2386
NcoI CCATGG 1 cut(s) 1563
NdeII GATC 8 cut(s) 910, 914, 1312, 1714, 2035, 2182, 2521, 3098
NgoMIV GCCGGC 1 cut(s) 854
NlaIV GGNNCC 9 cut(s) 82, 151, 597, 620, 2066, 2523, 2703, 3078, 3150
NsbI TGCGCA 2 cut(s) 1379, 2472
NspI RCATGY 2 cut(s) 1219, 2222
OliI CACNNNNGTG 1 cut(s) 2828
PagI TCATGA 3 cut(s) 52, 1660, 1804
PalAI GGCGCGCC 1 cut(s) 228
PasI CCCWGGG 1 cut(s) 160
PauI GCGCGC 1 cut(s) 228
PceI AGGCCT 1 cut(s) 2051
PciI ACATGT 2 cut(s) 1215, 2218
PciSI GCTCTTC 1 cut(s) 1341
PcsI WCGNNNNNNNCGW 2 cut(s) 1690, 2631
PctI GAATGC 1 cut(s) 3053
PdiI GCCGGC 1 cut(s) 856
PfeI GAWTC 8 cut(s) 797, 1276, 1307, 1737, 1753, 2003, 2393, 2677
Pfl23II CGTACG 1 cut(s) 2991
PflFI GACNNNGTC 1 cut(s) 2627
PfoI TCCNGGA 1 cut(s) 3118
PleI GAGTC 6 cut(s) 55, 97, 182, 295, 1352, 2828
PluTI GGCGCC 1 cut(s) 599
PmaCI CACGTG 2 cut(s) 971, 2923
PmlI CACGTG 2 cut(s) 971, 2923
PpsI GAGTC 6 cut(s) 55, 97, 182, 295, 1352, 2828
Ppu21I YACGTR 3 cut(s) 971, 2923, 2991
PscI ACATGT 2 cut(s) 1215, 2218
Psp124BI GAGCTC 1 cut(s) 3064
Psp6I CCWGG 8 cut(s) 159, 370, 576, 1790, 2190, 2818, 3118, 3169
PspCI CACGTG 2 cut(s) 971, 2923
PspEI GGTNACC 2 cut(s) 463, 1642
PspFI CCCAGC 3 cut(s) 62, 84, 504
PspGI CCWGG 8 cut(s) 159, 370, 576, 1790, 2190, 2818, 3118, 3169
PspLI CGTACG 1 cut(s) 2991
PspN4I GGNNCC 9 cut(s) 82, 151, 597, 620, 2066, 2523, 2703, 3078, 3150
PstI CTGCAG 3 cut(s) 2879, 2964, 3187
PsuI RGATCY 3 cut(s) 1714, 2182, 2521
PsyI GACNNNGTC 1 cut(s) 2627
PteI GCGCGC 1 cut(s) 228
PvuII CAGCTG 3 cut(s) 778, 2414, 2951
RseI CAYNNNNRTG 2 cut(s) 714, 2828
SacI GAGCTC 1 cut(s) 3064
SacII CCGCGG 2 cut(s) 202, 3155
SapI GCTCTTC 1 cut(s) 1341
SaqAI TTAA 8 cut(s) 756, 885, 1326, 1403, 1638, 1908, 1995, 2054
Sau3AI GATC 8 cut(s) 910, 914, 1312, 1714, 2035, 2182, 2521, 3098
ScaI AGTACT 1 cut(s) 1475
SchI GAGTC 6 cut(s) 56, 97, 183, 295, 1353, 2828
SduI GDGCHC 8 cut(s) 94, 571, 2029, 2537, 3064, 3118, 3161, 3169
SexAI ACCWGGT 1 cut(s) 3169
SfaNI GCATC 6 cut(s) 1009, 2062, 2296, 2616, 2662, 2990
SfcI CTRYAG 3 cut(s) 2875, 2960, 3183
SfoI GGCGCC 1 cut(s) 597
Sfr303I CCGCGG 2 cut(s) 202, 3155
SgrBI CCGCGG 2 cut(s) 202, 3155
SgsI GGCGCGCC 1 cut(s) 228
SinI GGWCC 6 cut(s) 149, 296, 359, 377, 3149, 3173
SmaI CCCGGG 2 cut(s) 633, 2386
SmiMI CAYNNNNRTG 2 cut(s) 714, 2828
SmlI CTYRAG 3 cut(s) 601, 1026, 1637
SmoI CTYRAG 3 cut(s) 601, 1026, 1637
SnaBI TACGTA 1 cut(s) 2991
SseBI AGGCCT 1 cut(s) 2051
SspDI GGCGCC 1 cut(s) 595
SspI AATATT 1 cut(s) 1210
SspMI CTAG 4 cut(s) 30, 573, 1077, 2061
SstI GAGCTC 1 cut(s) 3064
StuI AGGCCT 1 cut(s) 2051
StyI CCWWGG 3 cut(s) 1563, 1725, 2697
TaiI ACGT 8 cut(s) 817, 973, 1409, 1938, 2323, 2749, 2925, 2993
TaqI TCGA 4 cut(s) 913, 1584, 2029, 2482
TaqII GACCGA 1 cut(s) 313
TatI WGTACW 3 cut(s) 659, 789, 1473
TauI GCSGC 2 cut(s) 201, 1549
TfiI GAWTC 8 cut(s) 797, 1276, 1307, 1737, 1753, 2003, 2393, 2677
Tru1I TTAA 8 cut(s) 756, 885, 1326, 1403, 1638, 1908, 1995, 2054
Tru9I TTAA 8 cut(s) 756, 885, 1326, 1403, 1638, 1908, 1995, 2054
TseI GCWGC 8 cut(s) 217, 403, 775, 965, 2411, 2877, 3050, 3182
TspGWI ACGGA 3 cut(s) 2195, 2567, 2870
TspMI CCCGGG 2 cut(s) 631, 2384
Tth111I GACNNNGTC 1 cut(s) 2627
Vha464I CTTAAG 1 cut(s) 1637
VneI GTGCAC 1 cut(s) 567
VpaK11BI GGWCC 6 cut(s) 149, 296, 359, 377, 3149, 3173
XagI CCTNNNNNAGG 2 cut(s) 456, 875
XapI RAATTY 4 cut(s) 941, 959, 2169, 2889
XceI RCATGY 2 cut(s) 1219, 2222
XcmI CCANNNNNNNNNTGG 1 cut(s) 167
XmaI CCCGGG 2 cut(s) 631, 2384
XmiI GTMKAC 2 cut(s) 817, 848
XspI CTAG 4 cut(s) 30, 573, 1077, 2061
ZrmI AGTACT 1 cut(s) 1475
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.