Rroxscaffold_3G00275860

Belongs to the argonaute family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0001348
Physical Location & Seq
Forward (+)
67174412 .. 67180976
6565 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00275860.1

Sequence Viewer

Length: 3471 bp
ATGGTGAGGAAGAAGCGATGTGGTCAACTCCCTGGTAGCGGTGGAGCTGAAACCTATGAATCTCAGCAAGTAGCTGATCATCAGGCTTACCAGCAGCACAACCAAGAGGCAAGAGGAGGAGGAGGTTACCAACACCAAGCTGGAAGACCACCACAAGGAGCCAACAGAGGAGGAGGCTATGGCGGCGGCCGTGGGAATGGGTTTGGGAATGGAAATGGAAATGGAAATGGTGGGATGCCATATCAGTACCAGCAGCAGCAGTATGGCGGTCCCCCGCCCTCTGAAAATGGAGGCAGGGGGAGGGGACAGGGAGGAGGAAATCGTGGTGGTGGAGGTTACGGCAGCGGTGGTGGTGTTGGTGTTGGCTACGGCCGTGGGGGAGGATCTTCTTCCGGTGGCTATGGCCCGCCCAAAACATCAATTCCTGACCTGCACCAAGCGACCCCTGCTGCTCCATATGTTGCTGGGGTCAACCCTCAGCCTCAGCCCACAACACCATCTGTGTCTTCACCTTCACCTTCAGAACTGGTGGAGTCTTTTCAGCAGGTTTCTATTGGGCAACAAGTTGCTCCTCTTCCTGTGCAGGCAATCCAACCTATTCCCACCTCAAGTAAGTCGGTTAGGTTCCCTCTTCGCCCTGGCAGAGGCACCGTCGGCGCTAAATGTATTGTCAAGGCCAATCACTTCTTTGCTGAGTTACCTGACAAAGATCTGCACCAGTATGATGTTACTATAACACCAGACATTGCATCAAGGAGGTTGAATCGGGCTGTGATCAAGCAGCTGGTGGATTTGTACAGGCAATCCCAGCTTGGAAATAGGCTCCCTGCCTATGACGGACGGAAGAGTCTGTACACTGCTGGGCCGCTTCCTTTCTCATCCAAGGAGTTCAAGATCATGCTTGTTGATGATGATGATGGATCAGGCGGGCAAAGGAAGGAGAAGGAGAGAGAATTTAAAGTGGTGGTCAAGTTTGCTGCACGTGCTGACCTTCACCATCTAGAACTCTTTTTGATGGGGAGGCAAGCTGAGGCTCCTCAGGAAGCCCTTCAGGTTCTCGACATTGTTCTGCGTGAATTGCCTACAGCTAGTTACTTTCCTGTGGCACGGTCATTTTATTCCCCTGATCTGGGGAGAAGGCAGTCGCTTGGTGAGGGTTTGGAAAGTTGGCGTGGATTCTATCAGAGTATTCGTCCAACGCAGATGGGGCTGTCTCTAAATATTGATATGTCATCTACTGCTTTCATCGAGCCACTGCCAATCATTGAATTTGTCAATCAGCTACTCAATCGAGACATTTCATTTAGACAATTGTCAGATGCTGATCGCGTGAAGATCAAGAAAGCTTTACGAGGAATCAAAGTTGAAGTTACACACCGTGGACATATGCGCAGAAAATACCGTATTACTGGATTAACATCACAACCAACAAGAGAGCTGACTTTTCCACTTGATGAGAGAGGTACAATGAAATCTGTTGTTGACTACTTTCATGAAACTTATGGTTTCATTATTAAGCATACTCAGTTGCCGTGTCTACAAGTGGGAAATCAGCAGAGATCAAATTACTTGCCCATGGAGGTCTGTAAAATAGTTGAAGGTCAGAGGTACTCTAGGAGGTTGAATGAGAGACAGATTACTGCTTTGCTGAAGGTGACCTGCCAGCGTCCTCATGAAAGGGAACGTGATATTATCCAGACAGTTCGTCAAAACGCTTACCTTGAAGATCCTTATGCTCAGGAGTTTGGCATTAAAATCAGTGAGAATCTCACTTTGCTTGAAGCCCGTATTCTTCCTGCACCAAGAGTAAAGAAAAGGATTGCCAGCCTCGAAACGGATGGACCTGGATATCCTTTTGTATCAGTATGGATATGTACCCAATTAATGATGTCACTTCTGGTGAAACCTGTAATCCGCTTTTCACTGCATGATTACTCTTTCCCTGTTATGGAGATCCTAGGGATTCTTGTTAAGGCCTACTTTTGTTTCCCTGTGAGGGGGATCGTAGATAAGCAGGGAATCCAATTCTTGTTGCAGTTCTTTCTCTCCTGTTTTGGGGTTGCAGGGAGGATGAAGGGAGTGAAAGTGAAAGGCCTTGCCAAAGATTATGGGGCAATCAAAAATGGTCAATGGTGGCACAGTCAACAATTGGATGTGCATAAACTTTTCCGGGGCGTGCAAGATGCTGTTGCCCGTCGGTTCTGTAGTGAACTTGTTCAAATGTGCAACGTTTCTGGCATGGCATTTAATCCAGAACCAGTACTTCCACCTGTCACCGCTCATCCCGGTCATGTCGAGAGGGCCCTAAAATCTCGCTTTCATGAAGCACTGACCAAACTCCAGCCCCGTGGCCAGGAGCTTGAGCTACTTATTGCTATTTTGCCAGATAATAATGGCTCTCTTTATGGTGATTTGAAGCGAATTTGTGAGACGGATCTCGGCCTTGTTTCCCAATGCTGTTTAACCAAGCATGTATTTAAAAACAATAAGCAACAGTATTTGGCAAATGTAACATTGAAGATCAACGTGAAGGTCGGGGGAAGGAATACAGTGCTTGTTGATGCTCTGTCCAGGCGAATTCCTTTAGTCAGTGATCGACCTACCATCATTTTTGGTGCTGATGTCACCCATCCTCATCCTGGAGAGGATTCAAGTCCGTCAATCGCAGCGGTTGTGGCTTCTCAAGATTGGCCTGAAGTTACAAAGTATGCTGGTTTGGTTTCTGCTCAAACCCATCGACAGGAACTCATCCAAGATTTGTTCAAAACATGGCAGGATCCAGCAAAGGGGACAATGTATGGTGGCATGATCAAGGAACTGCTCATATCTTTCCGTAGATCAACTGGGCAAAAACCACAGCGGATCATATTCTACAGGGATGGTGTCAGTGAAGGACAGTTTTATCAAGTTTTGCTGTATGAACTTGATGCCATTCGTAAAGCATGTGCCTCCTTGGAGCCCGATTATCAGCCTCCGGTGACTTTTGTGGTGGTTCAAAAGCGCCATCATACTAGGTTGTTTGCTAACAACCATAATGACTCAAGATCAGTTGACAGAAGTGGGAATATATTACCTGGTACAGTTGTAGACTCCAAAATATGTCATCCAACTGAATTTGACTTCTTCCTGTGTAGCCATGCTGGAATTCAGGGCACGAGTCGTCCAGCCCATTACCATGTGCTATGGGATGAGAACAACTTTTCAGCTGATGGATTGCAGACCCTCACAAATAACCTTTGTTATACATATGCGAGTCCCCCTGCATACTATGCACATCTTGCTGCATTTCGAGCCCGTTTCTACTTGGAACCACAGACGTCAGAAGGTGGGTCGGTGACAAGTGGTGCTACCACAAATGGACGCGGTGGTGGCGGTGGTGGACGTAGGGCGCCGCTTGCTGCAAATTCTGCTGCTGTGAAGCCTTTACCTGCTCTCAAGGATAATGTCAAGAAGGTTATGTTCTACTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ArgoL1 ArgoL2 ArgoMid ArgoN Gly-rich_Ago1 PAZ Piwi

Protein Analysis

1156

Amino Acids

127.68

Weight (kDa)

9.44

Isoelectric Point (pI)

43.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArgoN PF16486 221 - 357 1.9e-36 N-terminal domain of argonaute
ArgoL1 PF08699 368 - 417 8.9e-21 Argonaute linker 1 domain
PAZ PF02170 423 - 549 1.7e-30 PAZ domain
ArgoL2 PF16488 559 - 604 1.7e-13 Argonaute linker 2 domain
Piwi PF02171 790 - 1101 7.5e-105 Piwi domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 846
AatII GACGTC 1 cut(s) 3320
Acc16I TGCGCA 1 cut(s) 1391
Acc36I ACCTGC 4 cut(s) 438, 535, 1667, 3436
AccB1I GGYRCC 2 cut(s) 647, 3388
AccBSI CCGCTC 1 cut(s) 2279
AccI GTMKAC 2 cut(s) 1537, 3087
AccII CGCG 2 cut(s) 1329, 3363
AclI AACGTT 1 cut(s) 2229
AclWI GGATC 9 cut(s) 391, 928, 1721, 1948, 2009, 2442, 2771, 2784, 2870
AcoI YGGCCR 3 cut(s) 187, 370, 2350
AcsI RAATTY 7 cut(s) 953, 1268, 2421, 2577, 3113, 3144, 3403
AcuI CTGAAG 4 cut(s) 504, 1034, 1670, 2715
AcvI CACGTG 1 cut(s) 983
AcyI GRCGYC 2 cut(s) 3317, 3389
AdeI CACNNNGTG 1 cut(s) 1379
AfaI GTAC 8 cut(s) 248, 797, 854, 1465, 1610, 1876, 2262, 3079
AhdI GACNNNNNGTC 1 cut(s) 1704
AjnI CCWGG 7 cut(s) 31, 637, 1843, 2352, 2570, 2638, 3073
Alw26I GTCTC 4 cut(s) 1218, 1287, 1624, 2423
AlwI GGATC 9 cut(s) 391, 928, 1721, 1948, 2009, 2442, 2771, 2784, 2870
AlwNI CAGNNNCTG 1 cut(s) 1322
ApaI GGGCCC 1 cut(s) 2305
ApoI RAATTY 7 cut(s) 953, 1268, 2421, 2577, 3113, 3144, 3403
AseI ATTAAT 1 cut(s) 1883
Asp700I GAANNNNTTC 2 cut(s) 1047, 2214
AspA2I CCTAGG 1 cut(s) 1957
AspLEI GCGC 4 cut(s) 659, 1392, 3003, 3391
AspS9I GGNCC 6 cut(s) 269, 404, 863, 1841, 2301, 2302
AsuC2I CCSGG 2 cut(s) 2171, 2286
AvaII GGWCC 2 cut(s) 269, 1841
AvrII CCTAGG 1 cut(s) 1957
AxyI CCTNAGG 1 cut(s) 1038
BaeGI GKGCMC 2 cut(s) 2305, 3155
BaeI ACNNNNGTAYC 2 cut(s) 3069, 3102
BalI TGGCCA 1 cut(s) 2352
BamHI GGATCC 1 cut(s) 2776
BanI GGYRCC 2 cut(s) 647, 3388
BanII GRGCYC 3 cut(s) 2305, 2961, 3295
BarI GAAGNNNNNNTAC 2 cut(s) 836, 868
BauI CACGAG 1 cut(s) 3154
BbrPI CACGTG 1 cut(s) 983
BbsI GAAGAC 2 cut(s) 151, 498
BbvCI CCTCAGC 3 cut(s) 477, 483, 1029
BceAI ACGGC 5 cut(s) 174, 355, 357, 385, 1516
BciT130I CCWGG 7 cut(s) 33, 639, 1845, 2354, 2572, 2640, 3075
BclI TGATCA 3 cut(s) 76, 774, 2808
BcnI CCSGG 2 cut(s) 2171, 2286
BcoDI GTCTC 4 cut(s) 1218, 1287, 1624, 2423
BfaI CTAG 5 cut(s) 1001, 1089, 1614, 1958, 3012
BfmI CTRYAG 3 cut(s) 1083, 2203, 2872
BfoI RGCGCY 3 cut(s) 660, 3004, 3392
BfuAI ACCTGC 4 cut(s) 438, 535, 1667, 3436
BglII AGATCT 1 cut(s) 709
BlnI CCTAGG 1 cut(s) 1957
BmcAI AGTACT 1 cut(s) 2262
Bme1390I CCNGG 9 cut(s) 33, 639, 1845, 2171, 2286, 2354, 2572, 2640, 3075
Bme18I GGWCC 2 cut(s) 269, 1841
BmeRI GACNNNNNGTC 1 cut(s) 1704
BmgT120I GGNCC 6 cut(s) 269, 404, 863, 1841, 2301, 2302
BmrFI CCNGG 9 cut(s) 33, 639, 1845, 2171, 2286, 2354, 2572, 2640, 3075
BmrI ACTGGG 1 cut(s) 2853
BmsI GCATC 6 cut(s) 225, 758, 1309, 2173, 2551, 2917
BmuI ACTGGG 1 cut(s) 2853
BoxI GACNNNNGTC 1 cut(s) 1312
BpiI GAAGAC 2 cut(s) 151, 498
BpmI CTGGAG 2 cut(s) 2324, 2661
Bpu10I CCTNAGC 4 cut(s) 477, 483, 1029, 1737
BpuEI CTTGAG 5 cut(s) 592, 2381, 2667, 3025, 3419
BpuMI CCSGG 2 cut(s) 2171, 2286
BsaAI YACGTR 1 cut(s) 983
BsaBI GATNNNNATC 2 cut(s) 1323, 1417
BsaHI GRCGYC 2 cut(s) 3317, 3389
BsaWI WCCGGW 2 cut(s) 392, 2974
BsaXI ACNNNNNCTCC 2 cut(s) 2070, 2100
Bse1I ACTGG 5 cut(s) 531, 718, 1414, 2258, 2848
Bse21I CCTNAGG 1 cut(s) 1038
Bse3DI GCAATG 1 cut(s) 744
Bse8I GATNNNNATC 2 cut(s) 1323, 1417
BseBI CCWGG 7 cut(s) 33, 639, 1845, 2354, 2572, 2640, 3075
BseJI GATNNNNATC 2 cut(s) 1323, 1417
BseMI GCAATG 1 cut(s) 744
BseMII CTCAG 8 cut(s) 77, 491, 497, 684, 1020, 1052, 1538, 1751
BseNI ACTGG 5 cut(s) 531, 718, 1414, 2258, 2848
BseRI GAGGAG 8 cut(s) 129, 132, 135, 183, 186, 327, 561, 1026
BseSI GKGCMC 2 cut(s) 2305, 3155
BseX3I CGGCCG 2 cut(s) 187, 370
BseYI CCCAGC 3 cut(s) 464, 807, 860
BsgI GTGCAG 5 cut(s) 416, 602, 698, 963, 1782
Bsh1236I CGCG 2 cut(s) 1329, 3363
Bsh1285I CGRYCG 2 cut(s) 190, 373
BshNI GGYRCC 2 cut(s) 647, 3388
BsiEI CGRYCG 2 cut(s) 190, 373
BsiSI CCGG 4 cut(s) 393, 2170, 2286, 2975
BslFI GGGAC 4 cut(s) 255, 318, 2803, 3240
BsmAI GTCTC 4 cut(s) 1218, 1287, 1624, 2423
BsmBI CGTCTC 1 cut(s) 2423
BsmFI GGGAC 4 cut(s) 255, 318, 2803, 3240
Bsp120I GGGCCC 1 cut(s) 2301
Bsp1286I GDGCHC 4 cut(s) 2305, 2961, 3155, 3295
Bsp1407I TGTACA 2 cut(s) 795, 852
Bsp19I CCATGG 1 cut(s) 1575
BspCNI CTCAG 8 cut(s) 76, 490, 496, 685, 1021, 1051, 1537, 1750
BspFNI CGCG 2 cut(s) 1329, 3363
BspHI TCATGA 3 cut(s) 1492, 1672, 2320
BspMI ACCTGC 4 cut(s) 438, 535, 1667, 3436
BspPI GGATC 9 cut(s) 391, 928, 1721, 1948, 2009, 2442, 2771, 2784, 2870
BspT107I GGYRCC 2 cut(s) 647, 3388
BsrBI CCGCTC 1 cut(s) 2279
BsrDI GCAATG 1 cut(s) 744
BsrGI TGTACA 2 cut(s) 795, 852
BsrI ACTGG 5 cut(s) 531, 718, 1414, 2258, 2848
BssNI GRCGYC 2 cut(s) 3317, 3389
BssSI CACGAG 1 cut(s) 3154
BssT1I CCWWGG 4 cut(s) 882, 1575, 1957, 2952
Bst2BI CACGAG 1 cut(s) 3154
Bst2UI CCWGG 7 cut(s) 33, 639, 1845, 2354, 2572, 2640, 3075
Bst6I CTCTTC 3 cut(s) 579, 636, 839
BstACI GRCGYC 2 cut(s) 3317, 3389
BstAPI GCANNNNNTGC 3 cut(s) 3269, 3278, 3407
BstAUI TGTACA 2 cut(s) 795, 852
BstBAI YACGTR 1 cut(s) 983
BstC8I GCNNGC 8 cut(s) 407, 585, 929, 1026, 1664, 1825, 2177, 3396
BstDEI CTNAG 8 cut(s) 63, 477, 483, 693, 1029, 1038, 1524, 1737
BstDSI CCRYGG 5 cut(s) 190, 373, 1378, 1575, 2347
BstEII GGTNACC 2 cut(s) 125, 1654
BstENI CCTNNNNNAGG 1 cut(s) 642
BstFNI CGCG 2 cut(s) 1329, 3363
BstH2I RGCGCY 3 cut(s) 660, 3004, 3392
BstHHI GCGC 4 cut(s) 659, 1392, 3003, 3391
BstMAI GTCTC 4 cut(s) 1218, 1287, 1624, 2423
BstMCI CGRYCG 2 cut(s) 190, 373
BstNI CCWGG 7 cut(s) 33, 639, 1845, 2354, 2572, 2640, 3075
BstNSI RCATGY 2 cut(s) 2474, 2946
BstPAI GACNNNNGTC 1 cut(s) 1312
BstPI GGTNACC 2 cut(s) 125, 1654
BstSCI CCNGG 9 cut(s) 31, 637, 1843, 2169, 2284, 2352, 2570, 2638, 3073
BstSFI CTRYAG 3 cut(s) 1083, 2203, 2872
BstSLI GKGCMC 2 cut(s) 2305, 3155
BstUI CGCG 2 cut(s) 1329, 3363
BstV2I GAAGAC 2 cut(s) 151, 498
BstX2I RGATCY 6 cut(s) 383, 709, 1726, 1953, 2434, 2776
BstXI CCANNNNNNTGG 1 cut(s) 2348
BstYI RGATCY 6 cut(s) 383, 709, 1726, 1953, 2434, 2776
BstZI CGGCCG 2 cut(s) 187, 370
Bsu36I CCTNAGG 1 cut(s) 1038
BtgI CCRYGG 5 cut(s) 190, 373, 1378, 1575, 2347
BtgZI GCGATG 1 cut(s) 31
BtsI GCAGTG 3 cut(s) 855, 1253, 1922
BtsIMutI CAGTG 8 cut(s) 855, 1253, 1765, 1922, 2327, 2556, 2596, 2893
BveI ACCTGC 4 cut(s) 438, 535, 1667, 3436
Cac8I GCNNGC 8 cut(s) 407, 585, 929, 1026, 1664, 1825, 2177, 3396
CaiI CAGNNNCTG 1 cut(s) 1322
CciI TCATGA 3 cut(s) 1492, 1672, 2320
CfoI GCGC 4 cut(s) 659, 1392, 3003, 3391
Cfr13I GGNCC 6 cut(s) 269, 404, 863, 1841, 2301, 2302
CseI GACGC 2 cut(s) 1655, 3369
CsiI ACCWGGT 1 cut(s) 3073
Csp6I GTAC 8 cut(s) 247, 796, 853, 1464, 1609, 1875, 2261, 3078
CviQI GTAC 8 cut(s) 247, 796, 853, 1464, 1609, 1875, 2261, 3078
DdeI CTNAG 8 cut(s) 63, 477, 483, 693, 1029, 1038, 1524, 1737
DinI GGCGCC 1 cut(s) 3390
DraI TTTAAA 2 cut(s) 958, 2479
DraIII CACNNNGTG 1 cut(s) 1379
DrdI GACNNNNNNGTC 1 cut(s) 846
DriI GACNNNNNGTC 1 cut(s) 1704
DseDI GACNNNNNNGTC 1 cut(s) 846
EaeI YGGCCR 3 cut(s) 187, 370, 2350
EagI CGGCCG 2 cut(s) 187, 370
Eam1104I CTCTTC 3 cut(s) 579, 636, 839
Eam1105I GACNNNNNGTC 1 cut(s) 1704
EarI CTCTTC 3 cut(s) 579, 636, 839
EclXI CGGCCG 2 cut(s) 187, 370
Eco130I CCWWGG 4 cut(s) 882, 1575, 1957, 2952
Eco147I AGGCCT 2 cut(s) 1976, 2094
Eco24I GRGCYC 3 cut(s) 2305, 2961, 3295
Eco32I GATATC 1 cut(s) 1850
Eco47I GGWCC 2 cut(s) 269, 1841
Eco52I CGGCCG 2 cut(s) 187, 370
Eco57I CTGAAG 4 cut(s) 504, 1034, 1670, 2715
Eco72I CACGTG 1 cut(s) 983
Eco81I CCTNAGG 1 cut(s) 1038
Eco91I GGTNACC 2 cut(s) 125, 1654
EcoNI CCTNNNNNAGG 1 cut(s) 642
EcoO109I RGGNCCY 2 cut(s) 2301, 2302
EcoO65I GGTNACC 2 cut(s) 125, 1654
EcoRI GAATTC 2 cut(s) 2577, 3144
EcoRII CCWGG 7 cut(s) 31, 637, 1843, 2352, 2570, 2638, 3073
EcoRV GATATC 1 cut(s) 1850
EcoT14I CCWWGG 4 cut(s) 882, 1575, 1957, 2952
EcoT38I GRGCYC 3 cut(s) 2305, 2961, 3295
EgeI GGCGCC 1 cut(s) 3390
EheI GGCGCC 1 cut(s) 3390
ErhI CCWWGG 4 cut(s) 882, 1575, 1957, 2952
Esp3I CGTCTC 1 cut(s) 2423
FalI AAGNNNNNCTT 2 cut(s) 1964, 1996
FaqI GGGAC 4 cut(s) 255, 318, 2803, 3240
FauI CCCGC 3 cut(s) 282, 414, 920
FauNDI CATATG 3 cut(s) 457, 1386, 3247
FbaI TGATCA 3 cut(s) 76, 774, 2808
FblI GTMKAC 2 cut(s) 1537, 3087
FriOI GRGCYC 3 cut(s) 2305, 2961, 3295
FspBI CTAG 5 cut(s) 1001, 1089, 1614, 1958, 3012
FspI TGCGCA 1 cut(s) 1391
GlaI GCGC 4 cut(s) 658, 1391, 3002, 3390
GsaI CCCAGC 3 cut(s) 468, 811, 864
GsuI CTGGAG 2 cut(s) 2324, 2661
HaeII RGCGCY 3 cut(s) 660, 3004, 3392
HapII CCGG 4 cut(s) 393, 2170, 2286, 2975
HgaI GACGC 2 cut(s) 1655, 3369
HhaI GCGC 4 cut(s) 659, 1392, 3003, 3391
Hin1I GRCGYC 2 cut(s) 3317, 3389
Hin6I GCGC 4 cut(s) 657, 1390, 3001, 3389
HinP1I GCGC 4 cut(s) 657, 1390, 3001, 3389
HincII GTYRAC 5 cut(s) 26, 472, 1483, 2144, 3052
HindII GTYRAC 5 cut(s) 26, 472, 1483, 2144, 3052
HindIII AAGCTT 1 cut(s) 1344
HpaII CCGG 4 cut(s) 393, 2170, 2286, 2975
Hpy188I TCNGA 6 cut(s) 283, 523, 1185, 1318, 1605, 3322
Hpy99I CGWCG 2 cut(s) 656, 2199
HpyCH4IV ACGT 6 cut(s) 982, 1684, 2229, 2526, 3317, 3382
HpyF3I CTNAG 8 cut(s) 63, 477, 483, 693, 1029, 1038, 1524, 1737
HpySE526I ACGT 6 cut(s) 982, 1684, 2229, 2526, 3317, 3382
Hsp92I GRCGYC 2 cut(s) 3317, 3389
HspAI GCGC 4 cut(s) 657, 1390, 3001, 3389
KasI GGCGCC 1 cut(s) 3388
Ksp22I TGATCA 3 cut(s) 76, 774, 2808
LmnI GCTCC 8 cut(s) 44, 158, 457, 574, 828, 1039, 2356, 2956
LweI GCATC 6 cut(s) 225, 758, 1309, 2173, 2551, 2917
MabI ACCWGGT 1 cut(s) 3073
MaeI CTAG 5 cut(s) 1001, 1089, 1614, 1958, 3012
MaeII ACGT 6 cut(s) 982, 1684, 2229, 2526, 3317, 3382
MbiI CCGCTC 1 cut(s) 2279
MfeI CAATTG 2 cut(s) 1310, 2147
MflI RGATCY 6 cut(s) 383, 709, 1726, 1953, 2434, 2776
MhlI GDGCHC 4 cut(s) 2305, 2961, 3155, 3295
MlsI TGGCCA 1 cut(s) 2352
MluNI TGGCCA 1 cut(s) 2352
Mly113I GGCGCC 1 cut(s) 3389
MlyI GAGTC 6 cut(s) 542, 856, 3032, 3083, 3166, 3262
MmeI TCCRAC 3 cut(s) 616, 1220, 3131
Mox20I TGGCCA 1 cut(s) 2352
MroXI GAANNNNTTC 2 cut(s) 1047, 2214
MscI TGGCCA 1 cut(s) 2352
MseI TTAA 9 cut(s) 957, 1415, 1515, 1752, 1883, 1971, 2247, 2462, 2478
MslI CAYNNNNRTG 2 cut(s) 720, 3174
Msp20I TGGCCA 1 cut(s) 2352
MspA1I CMGCKG 5 cut(s) 345, 784, 2669, 2860, 3206
MspI CCGG 4 cut(s) 393, 2170, 2286, 2975
MspR9I CCNGG 9 cut(s) 33, 639, 1845, 2171, 2286, 2354, 2572, 2640, 3075
MunI CAATTG 2 cut(s) 1310, 2147
MvaI CCWGG 7 cut(s) 33, 639, 1845, 2354, 2572, 2640, 3075
MvnI CGCG 2 cut(s) 1329, 3363
NarI GGCGCC 1 cut(s) 3389
NciI CCSGG 2 cut(s) 2171, 2286
NcoI CCATGG 1 cut(s) 1575
NdeI CATATG 3 cut(s) 457, 1386, 3247
NmeAIII GCCGAG 1 cut(s) 2418
NmuCI GTSAC 6 cut(s) 1654, 1890, 2272, 2623, 2977, 3334
NsbI TGCGCA 1 cut(s) 1391
NspI RCATGY 2 cut(s) 2474, 2946
PagI TCATGA 3 cut(s) 1492, 1672, 2320
PceI AGGCCT 2 cut(s) 1976, 2094
PdmI GAANNNNTTC 2 cut(s) 1047, 2214
PfeI GAWTC 8 cut(s) 59, 763, 1176, 1356, 1765, 1963, 2019, 2648
PfoI TCCNGGA 1 cut(s) 2638
PleI GAGTC 6 cut(s) 541, 855, 3032, 3083, 3165, 3261
PluTI GGCGCC 1 cut(s) 3392
PmaCI CACGTG 1 cut(s) 983
PmlI CACGTG 1 cut(s) 983
PpsI GAGTC 6 cut(s) 541, 855, 3032, 3083, 3165, 3261
Ppu21I YACGTR 1 cut(s) 983
PshAI GACNNNNGTC 1 cut(s) 1312
PshBI ATTAAT 1 cut(s) 1883
Psp1406I AACGTT 1 cut(s) 2229
Psp6I CCWGG 7 cut(s) 31, 637, 1843, 2352, 2570, 2638, 3073
PspCI CACGTG 1 cut(s) 983
PspEI GGTNACC 2 cut(s) 125, 1654
PspFI CCCAGC 3 cut(s) 464, 807, 860
PspGI CCWGG 7 cut(s) 31, 637, 1843, 2352, 2570, 2638, 3073
PspOMI GGGCCC 1 cut(s) 2301
PspPI GGNCC 6 cut(s) 269, 404, 863, 1841, 2301, 2302
PstNI CAGNNNCTG 1 cut(s) 1322
PsuI RGATCY 6 cut(s) 383, 709, 1726, 1953, 2434, 2776
PvuII CAGCTG 2 cut(s) 784, 3206
RsaI GTAC 8 cut(s) 248, 797, 854, 1465, 1610, 1876, 2262, 3079
RsaNI GTAC 8 cut(s) 247, 796, 853, 1464, 1609, 1875, 2261, 3078
RseI CAYNNNNRTG 2 cut(s) 720, 3174
SaqAI TTAA 9 cut(s) 957, 1415, 1515, 1752, 1883, 1971, 2247, 2462, 2478
Sau96I GGNCC 6 cut(s) 269, 404, 863, 1841, 2301, 2302
ScaI AGTACT 1 cut(s) 2262
SchI GAGTC 6 cut(s) 542, 856, 3032, 3083, 3166, 3262
ScrFI CCNGG 9 cut(s) 33, 639, 1845, 2171, 2286, 2354, 2572, 2640, 3075
SduI GDGCHC 4 cut(s) 2305, 2961, 3155, 3295
SexAI ACCWGGT 1 cut(s) 3073
SfaNI GCATC 6 cut(s) 225, 758, 1309, 2173, 2551, 2917
SfcI CTRYAG 3 cut(s) 1083, 2203, 2872
SfoI GGCGCC 1 cut(s) 3390
SinI GGWCC 2 cut(s) 269, 1841
SmiMI CAYNNNNRTG 2 cut(s) 720, 3174
SmlI CTYRAG 5 cut(s) 607, 2360, 2682, 3040, 3434
SmoI CTYRAG 5 cut(s) 607, 2360, 2682, 3040, 3434
SseBI AGGCCT 2 cut(s) 1976, 2094
SspDI GGCGCC 1 cut(s) 3388
SspI AATATT 1 cut(s) 1222
SspMI CTAG 5 cut(s) 1001, 1089, 1614, 1958, 3012
StuI AGGCCT 2 cut(s) 1976, 2094
StyD4I CCNGG 9 cut(s) 31, 637, 1843, 2169, 2284, 2352, 2570, 2638, 3073
StyI CCWWGG 4 cut(s) 882, 1575, 1957, 2952
TaiI ACGT 6 cut(s) 985, 1687, 2232, 2529, 3320, 3385
TaqI TCGA 8 cut(s) 1059, 1248, 1291, 1830, 2295, 2596, 2737, 3289
TatI WGTACW 3 cut(s) 795, 852, 2260
TauI GCSGC 4 cut(s) 186, 189, 868, 3394
TfiI GAWTC 8 cut(s) 59, 763, 1176, 1356, 1765, 1963, 2019, 2648
Tru1I TTAA 9 cut(s) 957, 1415, 1515, 1752, 1883, 1971, 2247, 2462, 2478
Tru9I TTAA 9 cut(s) 957, 1415, 1515, 1752, 1883, 1971, 2247, 2462, 2478
TscAI CASTG 8 cut(s) 862, 1260, 1765, 1929, 2334, 2556, 2596, 2893
TseFI GTSAC 6 cut(s) 1654, 1890, 2272, 2623, 2977, 3334
Tsp45I GTSAC 6 cut(s) 1654, 1890, 2272, 2623, 2977, 3334
TspGWI ACGGA 6 cut(s) 852, 856, 1850, 2447, 2646, 2822
TspRI CASTG 8 cut(s) 862, 1260, 1765, 1929, 2334, 2556, 2596, 2893
VpaK11BI GGWCC 2 cut(s) 269, 1841
VspI ATTAAT 1 cut(s) 1883
XagI CCTNNNNNAGG 1 cut(s) 642
XapI RAATTY 7 cut(s) 953, 1268, 2421, 2577, 3113, 3144, 3403
XbaI TCTAGA 1 cut(s) 1000
XceI RCATGY 2 cut(s) 2474, 2946
XcmI CCANNNNNNNNNTGG 2 cut(s) 137, 2636
XmaJI CCTAGG 1 cut(s) 1957
XmiI GTMKAC 2 cut(s) 1537, 3087
XmnI GAANNNNTTC 2 cut(s) 1047, 2214
XspI CTAG 5 cut(s) 1001, 1089, 1614, 1958, 3012
ZraI GACGTC 1 cut(s) 3318
ZrmI AGTACT 1 cut(s) 2262
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.