Rroxscaffold_3G00275870

Belongs to the argonaute family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0001348
Physical Location & Seq
Forward (+)
67183372 .. 67190055
6684 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00275870.1

Sequence Viewer

Length: 2988 bp
ATGAAGAGGCGAGCAGGGGCTCATTCTGATATTGATGTGCAAAAATACTCATGGTTATTTGAATCCCTAGTTTGCGTATTATTTATAGGAGAACTGAGCTTCCTAGCGGAAGTGAAAGCTCTGAGTCTGGTTCCCAGCGTCCCACTGCTCCACCAGCACAACAACAACAAGGTGCCGGAGGTCCCCAAGGGGGAAGGGGATCTCCGCCTCAACAGCAGGGTGGAGGGGGTTACCCACAACAACAAGGTGGAGGAGTTTACCAAAGTGGAAGGGGAGCTCCGCCTCAACAACAGGGTGGAGGTTACCAAGGTGGAAGAGGATCTACTCCACAGGGAGGGCGTGGGGGTTATGGTGGTGGCCGTGGAGGCTATCCAACGCCACAACAGCAGTATGGCGGCCCTCCTGAATATCAAGGCAGAGGCAGGGGAGGGCCTCCTCAGCAAGGAGGCCGAGGAGGGTACAGTGGCGGTCGTGGAGGACCTTCTCCTGCCTGCTTATCAAGCAAGTTCATCATCTCATCCACCTGAGCCTTCAGAAGTGGCAGAGCAGTTTCAGCAGGTTTCCATCCAGCAAGAGGAAGCTCCTAGTCAGGCCATTCAACGCGTTCCAGTTTCTAGCAAGGGTATGAGGTTCCCTCTTCGCCCTGGTAAGGGTAAGACTGGCACAAGGTGTACAGTCAAGGCAAATCATTTCTTTGCTGAATTACCAGACAAGGATCTGCACCAATATGATGTAACTATTACACCAGAAGTCACATCACGGGGTGTAAACCGTGCTGTGATGTCTCAGCTAGTGAAAACCTACAAGGACTCCCATCTTTCAAGCCGGCTTCCTGCTTATGATGGACGGAAGAGCCTGTATACTGCTGGACCCCTTCCTTTCCAGTCGAAGGAGTTCAAAATCACTCTTCTGGATGATGAGGAGGGACAGGCTGGACAAGCCCGGCGAGAGAGAGATTTCAGAGTTGTGATTAAATTCGCTGCTCGTGCTGACCTGCACCATTTAGGTCTCTTTTTGCAAGGGAGGATAGCTGATGCACCCCAGGAGGCCCTTCAGGTTCTTGACATTGTTCTAAGGGAGTTGCCTACTTCTAGGTACTGTCCTGTGGGGCGTTCATTTTATGACCCTGGCTTGGGGAGAAGACAACCACTTGGTGAGGGTTTGGAAAGCTGGCGTGGTTTCTACCAGAGTATTCGTCCAACTCAAATGGGTCTATCTTTAAATATTGATATGTCCTCGACAGCTTTTATTGAGCCATTGCCAGTTATTGAGTTTGTAACCCAGCTACTGAATCGTGATGTTCAAGCGAGGCCACTTTCTGATTCTGATCGTGTAAAGATTAAAAAGGCTCTACGTGGAGTGAGGGTTGAAGTTACACACCGGGGAAATATGCGCAGAAAATACCGCATCTCTGGTTTAACGTCCCAGGCAACAAGAGAATTAACTTTTCCTGTGGACGAAAGAGGAACGATGAAATCGGTTGTTGAATACTTTCATGAAACCTACGGTTTTGCCATTCAGCATCCATATTGGCCTTGTCTTCAAGTTGGAAATCAACAGCGACCAAACTATTTACCCATGGAGGTCTGTAAAATTGTTGAGGGACAAAGGTACTCCAAGAGACTGAATGAGAGACAGATAACTGCTTTGCTCAAGGTGACCTGTCAGCGCCCTCAAGAAAGGGAGCGTGATATTATGCAGACAGTACGCCATAATGCCTATGCTGAAGATCCTTATGCACAGGAATTCGGAATTAAAATTAGTGAGAATCTGGCTCAGGTTGAAGCTCGTATTCTTCCTCCACCATGGCTTAAATACCATGATACTGGAAGGGAAAAGGATTGCCTGCCCCAAGTTGGACAGTGGAATATGATGAATAAGAAAATGGTAAATGGAGGAAAAGTAACCAATTGGATCTGCATAAATTTTTCCCGCAATGTACAGGACAGTGTGGCAAAAGGATTTTGTTATGAACTTGCTCAGATGTGTCACATATCTGGCATGGCATTTAACCCTGAGCCCGTGCTTCCACCAGTCAGTGCTCGACCTGATCAGGTGGAAAAGGTATTAAAAACTCGATACCATGATGCAATGACGAAGATTCCGGGCAAGGAGCTTGACCTTCTTGTTGTCATTTTGCCAGATAATAATGGCTCTCTTTATGGTGATTTAAAAAGGATCTGTGAGACGGATCTTGGCCTAGTATCCCAGTGCTGTTTAACCAAACATGTGTTTAGAATGAGTAAACAGTATTTGGCAAACGTTGCCTTGAAGATAAATGTGAAGGTCGGGGGACGGAACACAGTACTTGTTGATGCAATCTCCAGGCGTATTCCCTTGGTTGTGGCTTCTCAAGACTGGCCGGAAATTACAAAGTATGCTGGCTTAGTATGTGCTCAAGCCCATCGTCAGGAGCTCATCCAAGATTTGGATGGTGTCAGCGAGGGCCAATTCTACCAAGTTCTACTGTATGAACTTGATGCAATTCGTAAGGCCTGCGCTTCACTGGAGCCAAATTATCAGCCTCCTGTGACTTTTGTTGTTGTTCAAAAGCGTCATCATACAAGATTGTTTGCTGACAACCATCGTGATCGCAATGCAATTGACAGGAGTGGGAACATATTACCTGGTACGGTCGGTACAAGCCGCCCAGCTCATTACCATGTGCTGTGGGATGAAAATAAGTTCACAGCAGATGCACTCCAGTCCCTTACCAATAATCTTTGCTATACATATGCAAGGTGTACACGCTCAGTTTCAATCGTTCCTCCTGCATATTATGCTCATCTCGCGGCGTTCCGAGCGAGATTTTACATGGAGCCTGAGACCTCAGATAGCGGTTCAATGTCAAGTGGTGCTCATGGACGTGGAGGCGGCATGGCTGGTGCTGGTGGAGGACGGAGCACTCGAGCACCGGGTCCAAATGCTGCTGTGAGGCCTTTACCTGCACTGAAAGAGAATGTGAAGAAGGTCATGTTCTACTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ArgoL1 ArgoL2 ArgoMid ArgoN Gly-rich_Ago1 PAZ Piwi

Protein Analysis

995

Amino Acids

111.63

Weight (kDa)

8.89

Isoelectric Point (pI)

48.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArgoN PF16486 223 - 358 1.2e-37 N-terminal domain of argonaute
ArgoL1 PF08699 368 - 418 1.2e-21 Argonaute linker 1 domain
PAZ PF02170 424 - 550 3e-30 PAZ domain
ArgoL2 PF16488 560 - 606 6.8e-18 Argonaute linker 2 domain
ArgoMid PF16487 617 - 692 1.3e-09 Mid domain of argonaute
Piwi PF02171 709 - 778 8.6e-17 Piwi domain
Piwi PF02171 811 - 879 4.9e-20 Piwi domain
Piwi PF02171 879 - 939 6.2e-22 Piwi domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1394
Acc36I ACCTGC 3 cut(s) 547, 1002, 2953
AccB1I GGYRCC 1 cut(s) 172
AccB7I CCANNNNNTGG 1 cut(s) 2428
AccI GTMKAC 1 cut(s) 860
AccII CGCG 2 cut(s) 603, 2792
AclI AACGTT 1 cut(s) 2262
AclWI GGATC 7 cut(s) 207, 327, 723, 1724, 1922, 2186, 2199
AcoI YGGCCR 2 cut(s) 357, 2360
AcsI RAATTY 3 cut(s) 974, 1745, 1924
AcuI CTGAAG 3 cut(s) 516, 1037, 1746
AdeI CACNNNGTG 3 cut(s) 669, 764, 1154
AflIII ACRYGT 2 cut(s) 601, 2227
AjiI CACGTC 1 cut(s) 2867
AjnI CCWGG 6 cut(s) 643, 1041, 1126, 1425, 2324, 2626
AloI GAACNNNNNNTCC 2 cut(s) 84, 116
Alw21I GWGCWC 7 cut(s) 279, 2044, 2398, 2418, 2860, 2906, 2914
Alw26I GTCTC 6 cut(s) 789, 1013, 1615, 1627, 2180, 2819
AlwI GGATC 7 cut(s) 207, 327, 723, 1724, 1922, 2186, 2199
AlwNI CAGNNNCTG 1 cut(s) 1288
Ama87I CYCGRG 1 cut(s) 2907
ApeKI GCWGC 2 cut(s) 980, 2927
ApoI RAATTY 3 cut(s) 974, 1745, 1924
Asp700I GAANNNNTTC 2 cut(s) 893, 1491
AspLEI GCGC 3 cut(s) 1395, 1671, 2501
AspS9I GGNCC 8 cut(s) 181, 397, 430, 478, 869, 1048, 2446, 2918
AsuC2I CCSGG 4 cut(s) 943, 1382, 2106, 2916
AsuHPI GGTGA 3 cut(s) 1166, 1669, 2177
AvaI CYCGRG 1 cut(s) 2907
AvaII GGWCC 4 cut(s) 181, 478, 869, 2918
BanI GGYRCC 1 cut(s) 172
BanII GRGCYC 4 cut(s) 22, 279, 2022, 2418
BarI GAAGNNNNNNTAC 2 cut(s) 306, 338
BauI CACGAG 1 cut(s) 984
BbsI GAAGAC 2 cut(s) 1147, 1532
Bbv12I GWGCWC 7 cut(s) 279, 2044, 2398, 2418, 2860, 2906, 2914
BbvCI CCTCAGC 1 cut(s) 437
BbvI GCAGC 2 cut(s) 967, 2914
BccI CCATC 6 cut(s) 572, 822, 836, 2412, 2426, 2592
BceAI ACGGC 1 cut(s) 344
BciT130I CCWGG 6 cut(s) 645, 1043, 1128, 1427, 2326, 2628
BciVI GTATCC 1 cut(s) 2215
BclI TGATCA 1 cut(s) 2050
BcnI CCSGG 4 cut(s) 943, 1382, 2106, 2916
BcoDI GTCTC 6 cut(s) 789, 1013, 1615, 1627, 2180, 2819
BfaI CTAG 8 cut(s) 68, 104, 585, 615, 791, 1092, 2201, 2986
BfoI RGCGCY 1 cut(s) 1672
BfuAI ACCTGC 3 cut(s) 547, 1002, 2953
BfuI GTATCC 1 cut(s) 2215
BglI GCCNNNNNGGC 1 cut(s) 365
BisI GCNGC 6 cut(s) 396, 981, 2647, 2793, 2875, 2928
BlsI GCNGC 6 cut(s) 397, 982, 2648, 2794, 2876, 2929
BmcAI AGTACT 1 cut(s) 2307
Bme18I GGWCC 4 cut(s) 181, 478, 869, 2918
BmeT110I CYCGRG 1 cut(s) 2907
BmgBI CACGTC 1 cut(s) 2867
BmgT120I GGNCC 8 cut(s) 181, 397, 430, 478, 869, 1048, 2446, 2918
BmiI GGNNCC 8 cut(s) 132, 174, 183, 632, 871, 2511, 2820, 2919
BmrI ACTGGG 1 cut(s) 2203
BmsI GCATC 7 cut(s) 1024, 1416, 1531, 2077, 2305, 2470, 2686
BmuI ACTGGG 1 cut(s) 2203
BpiI GAAGAC 2 cut(s) 1147, 1532
BplI GAGNNNNNCTC 2 cut(s) 619, 651
BpmI CTGGAG 3 cut(s) 2308, 2528, 2687
Bpu10I CCTNAGC 4 cut(s) 437, 525, 1776, 2016
BpuEI CTTGAG 4 cut(s) 1637, 1659, 2337, 2382
BpuMI CCSGG 4 cut(s) 943, 1382, 2106, 2916
BsaAI YACGTR 1 cut(s) 1355
BsaBI GATNNNNATC 1 cut(s) 1326
BsaI GGTCTC 2 cut(s) 1013, 2819
BsaXI ACNNNNNCTCC 6 cut(s) 794, 824, 1350, 1380, 1887, 1917
Bse118I RCCGGY 1 cut(s) 825
Bse3DI GCAATG 4 cut(s) 1256, 1942, 2097, 2602
Bse8I GATNNNNATC 1 cut(s) 1326
BseBI CCWGG 6 cut(s) 645, 1043, 1128, 1427, 2326, 2628
BseGI GGATG 7 cut(s) 517, 564, 919, 1522, 2418, 2437, 2680
BseJI GATNNNNATC 1 cut(s) 1326
BseMI GCAATG 4 cut(s) 1256, 1942, 2097, 2602
BseRI GAGGAG 4 cut(s) 266, 425, 467, 935
BseXI GCAGC 2 cut(s) 967, 2914
BseYI CCCAGC 3 cut(s) 134, 1281, 2650
BsgI GTGCAG 3 cut(s) 704, 980, 2931
Bsh1236I CGCG 2 cut(s) 603, 2792
Bsh1285I CGRYCG 2 cut(s) 471, 2637
BshNI GGYRCC 1 cut(s) 172
BsiEI CGRYCG 2 cut(s) 471, 2637
BsiHKAI GWGCWC 7 cut(s) 279, 2044, 2398, 2418, 2860, 2906, 2914
BsiHKCI CYCGRG 1 cut(s) 2907
BsiSI CCGG 7 cut(s) 176, 826, 943, 1381, 2105, 2363, 2915
BslFI GGGAC 7 cut(s) 125, 167, 939, 1408, 1617, 2307, 2692
BsmAI GTCTC 6 cut(s) 789, 1013, 1615, 1627, 2180, 2819
BsmBI CGTCTC 1 cut(s) 2180
BsmFI GGGAC 7 cut(s) 125, 167, 939, 1408, 1617, 2307, 2692
Bso31I GGTCTC 2 cut(s) 1013, 2819
BsoBI CYCGRG 1 cut(s) 2907
Bsp1286I GDGCHC 9 cut(s) 22, 279, 2022, 2044, 2398, 2418, 2860, 2906, 2914
Bsp1407I TGTACA 3 cut(s) 671, 1939, 2744
Bsp19I CCATGG 2 cut(s) 1578, 1805
BspFNI CGCG 2 cut(s) 603, 2792
BspHI TCATGA 1 cut(s) 1495
BspLI GGNNCC 8 cut(s) 132, 174, 183, 632, 871, 2511, 2820, 2919
BspMI ACCTGC 3 cut(s) 547, 1002, 2953
BspPI GGATC 7 cut(s) 207, 327, 723, 1724, 1922, 2186, 2199
BspQI GCTCTTC 1 cut(s) 845
BspT107I GGYRCC 1 cut(s) 172
BspTNI GGTCTC 2 cut(s) 1013, 2819
BsrDI GCAATG 4 cut(s) 1256, 1942, 2097, 2602
BsrFI RCCGGY 1 cut(s) 825
BsrGI TGTACA 3 cut(s) 671, 1939, 2744
BssAI RCCGGY 1 cut(s) 825
BssNAI GTATAC 1 cut(s) 861
BssSI CACGAG 1 cut(s) 984
BssT1I CCWWGG 5 cut(s) 186, 306, 1578, 1805, 2337
Bst1107I GTATAC 1 cut(s) 861
Bst2BI CACGAG 1 cut(s) 984
Bst2UI CCWGG 6 cut(s) 645, 1043, 1128, 1427, 2326, 2628
Bst6I CTCTTC 4 cut(s) 309, 642, 845, 912
BstAPI GCANNNNNTGC 2 cut(s) 2264, 2780
BstAUI TGTACA 3 cut(s) 671, 1939, 2744
BstBAI YACGTR 1 cut(s) 1355
BstC8I GCNNGC 7 cut(s) 12, 492, 827, 1172, 1847, 2383, 2497
BstDSI CCRYGG 3 cut(s) 360, 1578, 1805
BstEII GGTNACC 3 cut(s) 229, 301, 1657
BstENI CCTNNNNNAGG 1 cut(s) 440
BstF5I GGATG 7 cut(s) 517, 564, 919, 1522, 2418, 2437, 2680
BstFNI CGCG 2 cut(s) 603, 2792
BstH2I RGCGCY 1 cut(s) 1672
BstHHI GCGC 3 cut(s) 1395, 1671, 2501
BstMAI GTCTC 6 cut(s) 789, 1013, 1615, 1627, 2180, 2819
BstMCI CGRYCG 2 cut(s) 471, 2637
BstNI CCWGG 6 cut(s) 645, 1043, 1128, 1427, 2326, 2628
BstNSI RCATGY 1 cut(s) 2231
BstPI GGTNACC 3 cut(s) 229, 301, 1657
BstUI CGCG 2 cut(s) 603, 2792
BstV1I GCAGC 2 cut(s) 967, 2914
BstV2I GAAGAC 2 cut(s) 1147, 1532
BstX2I RGATCY 7 cut(s) 199, 319, 715, 1729, 1914, 2178, 2191
BstXI CCANNNNNNTGG 1 cut(s) 1826
BstYI RGATCY 7 cut(s) 199, 319, 715, 1729, 1914, 2178, 2191
BstZ17I GTATAC 1 cut(s) 861
BsuI GTATCC 1 cut(s) 2215
BtgI CCRYGG 3 cut(s) 360, 1578, 1805
BtrI CACGTC 1 cut(s) 2867
BtsCI GGATG 7 cut(s) 517, 564, 919, 1522, 2418, 2437, 2680
BtsI GCAGTG 1 cut(s) 143
BtsIMutI CAGTG 8 cut(s) 143, 468, 1868, 1954, 2044, 2216, 2504, 2948
BveI ACCTGC 3 cut(s) 547, 1002, 2953
Cac8I GCNNGC 7 cut(s) 12, 492, 827, 1172, 1847, 2383, 2497
CaiI CAGNNNCTG 1 cut(s) 1288
CciI TCATGA 1 cut(s) 1495
CfoI GCGC 3 cut(s) 1395, 1671, 2501
Cfr10I RCCGGY 1 cut(s) 825
Cfr13I GGNCC 8 cut(s) 181, 397, 430, 478, 869, 1048, 2446, 2918
CseI GACGC 2 cut(s) 127, 2543
CsiI ACCWGGT 1 cut(s) 2626
DraI TTTAAA 2 cut(s) 1221, 2172
DraIII CACNNNGTG 3 cut(s) 669, 764, 1154
EaeI YGGCCR 2 cut(s) 357, 2360
Eam1104I CTCTTC 4 cut(s) 309, 642, 845, 912
EarI CTCTTC 4 cut(s) 309, 642, 845, 912
EciI GGCGGA 2 cut(s) 194, 269
Ecl136II GAGCTC 2 cut(s) 277, 2416
Eco130I CCWWGG 5 cut(s) 186, 306, 1578, 1805, 2337
Eco147I AGGCCT 2 cut(s) 2495, 2938
Eco24I GRGCYC 4 cut(s) 22, 279, 2022, 2418
Eco31I GGTCTC 2 cut(s) 1013, 2819
Eco47I GGWCC 4 cut(s) 181, 478, 869, 2918
Eco53kI GAGCTC 2 cut(s) 277, 2416
Eco57I CTGAAG 3 cut(s) 516, 1037, 1746
Eco88I CYCGRG 1 cut(s) 2907
Eco91I GGTNACC 3 cut(s) 229, 301, 1657
EcoICRI GAGCTC 2 cut(s) 277, 2416
EcoNI CCTNNNNNAGG 1 cut(s) 440
EcoO109I RGGNCCY 4 cut(s) 181, 430, 478, 1048
EcoO65I GGTNACC 3 cut(s) 229, 301, 1657
EcoRI GAATTC 1 cut(s) 1745
EcoRII CCWGG 6 cut(s) 643, 1041, 1126, 1425, 2324, 2626
EcoT14I CCWWGG 5 cut(s) 186, 306, 1578, 1805, 2337
EcoT38I GRGCYC 4 cut(s) 22, 279, 2022, 2418
ErhI CCWWGG 5 cut(s) 186, 306, 1578, 1805, 2337
Esp3I CGTCTC 1 cut(s) 2180
FaqI GGGAC 7 cut(s) 125, 167, 939, 1408, 1617, 2307, 2692
FauI CCCGC 1 cut(s) 1940
FauNDI CATATG 1 cut(s) 2734
FbaI TGATCA 1 cut(s) 2050
FblI GTMKAC 1 cut(s) 860
Fnu4HI GCNGC 6 cut(s) 396, 981, 2647, 2793, 2875, 2928
FokI GGATG 7 cut(s) 504, 551, 926, 1509, 2405, 2444, 2687
FriOI GRGCYC 4 cut(s) 22, 279, 2022, 2418
Fsp4HI GCNGC 6 cut(s) 396, 981, 2647, 2793, 2875, 2928
FspBI CTAG 8 cut(s) 68, 104, 585, 615, 791, 1092, 2201, 2986
FspI TGCGCA 1 cut(s) 1394
GlaI GCGC 3 cut(s) 1394, 1670, 2500
GluI GCNGC 6 cut(s) 396, 981, 2647, 2793, 2875, 2928
GsaI CCCAGC 3 cut(s) 138, 1285, 2654
GsuI CTGGAG 3 cut(s) 2308, 2528, 2687
HaeII RGCGCY 1 cut(s) 1672
HapII CCGG 7 cut(s) 176, 826, 943, 1381, 2105, 2363, 2915
HgaI GACGC 2 cut(s) 127, 2543
HhaI GCGC 3 cut(s) 1395, 1671, 2501
Hin6I GCGC 3 cut(s) 1393, 1669, 2499
HinP1I GCGC 3 cut(s) 1393, 1669, 2499
HinfI GANTC 7 cut(s) 62, 124, 809, 1291, 1322, 1768, 2101
HpaII CCGG 7 cut(s) 176, 826, 943, 1381, 2105, 2363, 2915
HphI GGTGA 3 cut(s) 1166, 1669, 2177
Hpy166II GTNNAC 9 cut(s) 258, 672, 769, 861, 1456, 2246, 2688, 2745, 2747
Hpy188III TCNNGA 9 cut(s) 403, 911, 1061, 1295, 1496, 1676, 2354, 2411, 2588
Hpy8I GTNNAC 9 cut(s) 258, 672, 769, 861, 1456, 2246, 2688, 2745, 2747
HpyCH4IV ACGT 4 cut(s) 1354, 1421, 2262, 2866
HpySE526I ACGT 4 cut(s) 1354, 1421, 2262, 2866
HspAI GCGC 3 cut(s) 1393, 1669, 2499
KroI GCCGGC 1 cut(s) 825
KroNI GCCGGC 1 cut(s) 827
Ksp22I TGATCA 1 cut(s) 2050
LguI GCTCTTC 1 cut(s) 845
Lsp1109I GCAGC 2 cut(s) 967, 2914
LweI GCATC 7 cut(s) 1024, 1416, 1531, 2077, 2305, 2470, 2686
MabI ACCWGGT 1 cut(s) 2626
MaeI CTAG 8 cut(s) 68, 104, 585, 615, 791, 1092, 2201, 2986
MaeII ACGT 4 cut(s) 1354, 1421, 2262, 2866
MfeI CAATTG 2 cut(s) 1909, 2601
MflI RGATCY 7 cut(s) 199, 319, 715, 1729, 1914, 2178, 2191
MhlI GDGCHC 9 cut(s) 22, 279, 2022, 2044, 2398, 2418, 2860, 2906, 2914
MluI ACGCGT 1 cut(s) 601
MlyI GAGTC 2 cut(s) 133, 803
MmeI TCCRAC 4 cut(s) 397, 1223, 1528, 1837
MroNI GCCGGC 1 cut(s) 825
MroXI GAANNNNTTC 2 cut(s) 893, 1491
MslI CAYNNNNRTG 3 cut(s) 726, 2661, 2865
MspI CCGG 7 cut(s) 176, 826, 943, 1381, 2105, 2363, 2915
MunI CAATTG 2 cut(s) 1909, 2601
MvaI CCWGG 6 cut(s) 645, 1043, 1128, 1427, 2326, 2628
MvnI CGCG 2 cut(s) 603, 2792
NaeI GCCGGC 1 cut(s) 827
NciI CCSGG 4 cut(s) 943, 1382, 2106, 2916
NcoI CCATGG 2 cut(s) 1578, 1805
NdeI CATATG 1 cut(s) 2734
NgoMIV GCCGGC 1 cut(s) 825
NlaIV GGNNCC 8 cut(s) 132, 174, 183, 632, 871, 2511, 2820, 2919
NmeAIII GCCGAG 1 cut(s) 475
NmuCI GTSAC 4 cut(s) 751, 1657, 1988, 2530
NsbI TGCGCA 1 cut(s) 1394
NspI RCATGY 1 cut(s) 2231
PaeR7I CTCGAG 1 cut(s) 2907
PagI TCATGA 1 cut(s) 1495
PceI AGGCCT 2 cut(s) 2495, 2938
PciI ACATGT 1 cut(s) 2227
PciSI GCTCTTC 1 cut(s) 845
PcsI WCGNNNNNNNCGW 1 cut(s) 2905
PdiI GCCGGC 1 cut(s) 827
PdmI GAANNNNTTC 2 cut(s) 893, 1491
PfeI GAWTC 5 cut(s) 62, 1291, 1322, 1768, 2101
PflMI CCANNNNNTGG 1 cut(s) 2428
PkrI GCNGC 6 cut(s) 397, 982, 2648, 2794, 2876, 2929
PleI GAGTC 2 cut(s) 132, 803
PpsI GAGTC 2 cut(s) 132, 803
Ppu21I YACGTR 1 cut(s) 1355
PpuMI RGGWCCY 2 cut(s) 181, 478
PscI ACATGT 1 cut(s) 2227
Psp124BI GAGCTC 2 cut(s) 279, 2418
Psp1406I AACGTT 1 cut(s) 2262
Psp5II RGGWCCY 2 cut(s) 181, 478
Psp6I CCWGG 6 cut(s) 643, 1041, 1126, 1425, 2324, 2626
PspEI GGTNACC 3 cut(s) 229, 301, 1657
PspFI CCCAGC 3 cut(s) 134, 1281, 2650
PspGI CCWGG 6 cut(s) 643, 1041, 1126, 1425, 2324, 2626
PspN4I GGNNCC 8 cut(s) 132, 174, 183, 632, 871, 2511, 2820, 2919
PspPI GGNCC 8 cut(s) 181, 397, 430, 478, 869, 1048, 2446, 2918
PspPPI RGGWCCY 2 cut(s) 181, 478
PspXI VCTCGAGB 1 cut(s) 2907
PstNI CAGNNNCTG 1 cut(s) 1288
PsuI RGATCY 7 cut(s) 199, 319, 715, 1729, 1914, 2178, 2191
RseI CAYNNNNRTG 3 cut(s) 726, 2661, 2865
SacI GAGCTC 2 cut(s) 279, 2418
SapI GCTCTTC 1 cut(s) 845
SatI GCNGC 6 cut(s) 396, 981, 2647, 2793, 2875, 2928
Sau96I GGNCC 8 cut(s) 181, 397, 430, 478, 869, 1048, 2446, 2918
ScaI AGTACT 1 cut(s) 2307
SchI GAGTC 2 cut(s) 133, 803
SduI GDGCHC 9 cut(s) 22, 279, 2022, 2044, 2398, 2418, 2860, 2906, 2914
SexAI ACCWGGT 1 cut(s) 2626
SfaNI GCATC 7 cut(s) 1024, 1416, 1531, 2077, 2305, 2470, 2686
Sfr274I CTCGAG 1 cut(s) 2907
SinI GGWCC 4 cut(s) 181, 478, 869, 2918
SlaI CTCGAG 1 cut(s) 2907
SmiMI CAYNNNNRTG 3 cut(s) 726, 2661, 2865
SmlI CTYRAG 5 cut(s) 1652, 1674, 2352, 2397, 2907
SmoI CTYRAG 5 cut(s) 1652, 1674, 2352, 2397, 2907
SseBI AGGCCT 2 cut(s) 2495, 2938
SspI AATATT 1 cut(s) 1225
SspMI CTAG 8 cut(s) 68, 104, 585, 615, 791, 1092, 2201, 2986
SstI GAGCTC 2 cut(s) 279, 2418
StuI AGGCCT 2 cut(s) 2495, 2938
StyI CCWWGG 5 cut(s) 186, 306, 1578, 1805, 2337
TaiI ACGT 4 cut(s) 1357, 1424, 2265, 2869
TaqI TCGA 5 cut(s) 887, 1238, 2044, 2077, 2908
TatI WGTACW 4 cut(s) 671, 1939, 2305, 2744
TauI GCSGC 4 cut(s) 398, 2649, 2795, 2877
TfiI GAWTC 5 cut(s) 62, 1291, 1322, 1768, 2101
TscAI CASTG 8 cut(s) 150, 468, 1868, 1954, 2044, 2216, 2511, 2955
TseFI GTSAC 4 cut(s) 751, 1657, 1988, 2530
TseI GCWGC 2 cut(s) 980, 2927
Tsp45I GTSAC 4 cut(s) 751, 1657, 1988, 2530
TspGWI ACGGA 4 cut(s) 862, 2204, 2311, 2914
TspRI CASTG 8 cut(s) 150, 468, 1868, 1954, 2044, 2216, 2511, 2955
Van91I CCANNNNNTGG 1 cut(s) 2428
VpaK11BI GGWCC 4 cut(s) 181, 478, 869, 2918
XagI CCTNNNNNAGG 1 cut(s) 440
XapI RAATTY 3 cut(s) 974, 1745, 1924
XceI RCATGY 1 cut(s) 2231
XcmI CCANNNNNNNNNTGG 1 cut(s) 2429
XhoI CTCGAG 1 cut(s) 2907
XmiI GTMKAC 1 cut(s) 860
XmnI GAANNNNTTC 2 cut(s) 893, 1491
XspI CTAG 8 cut(s) 68, 104, 585, 615, 791, 1092, 2201, 2986
ZrmI AGTACT 1 cut(s) 2307
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.