RchiOBHm_Chr7g0177451

Belongs to the argonaute family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0001348
Physical Location & Seq
Reverse (-)
416446 .. 418316
1871 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ15806

Sequence Viewer

Length: 591 bp
ATGGCAGGATCCAGCAAAGGGACAATGTCTGGTGGCATGATCAAGGAACTGCTCATATCTTTCCGTAGATCAACTGGGCAAAAACCACAACGGATCATATTCTACAGGGATGGTGTCAGTGAAGGACAGTTTTATCAAGTTTTGCTGTATGAACTTGATGCCATTCGTAAAGCATGTGCCTCCTTGGAGCCCGATTATCAGCCTCCAGTGACTTTTGTGGTGGTTCAAAAGCGCCATCATACTAGGTTGTTTGCTAACAACCATAATGACTCAAGATCAGTTGACAGAAGTGGGAATATATTACCTGGTACAGTTGTAGACTCCAAAATCTGTCATCCAACTGAATTTGACTTCTACCTGTGTAGCCATGCTGGAATTCAGGGCACGAGTCGTCCAGCCCATTACCATGTGCTATGGGATGAGAACAACTTTTCAGCTGATGGATTGCAGACCCTCACAAATAACCTTTGTTATACATATGCGAGGTGTACACGTTCTGTTTCTGTTGTCCCCCTGCATACTATGCACATCTTGCTGCATTTCGAGCCCGTTTCTACTTGGAACCACAGACGTCAGAAGGCGGGTCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ArgoL1 ArgoL2 ArgoMid ArgoN Gly-rich_Ago1 PAZ Piwi

Protein Analysis

196

Amino Acids

22.24

Weight (kDa)

9.02

Isoelectric Point (pI)

65.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Piwi PF02171 13 - 171 1.1e-69 Piwi domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 574
AccI GTMKAC 1 cut(s) 318
AciI CCGC 1 cut(s) 581
AclWI GGATC 3 cut(s) 3, 16, 101
AcsI RAATTY 2 cut(s) 344, 375
AcyI GRCGYC 1 cut(s) 571
AfaI GTAC 2 cut(s) 310, 490
AfiI CCNNNNNNNGG 1 cut(s) 18
AflIII ACRYGT 1 cut(s) 491
AgsI TTSAA 1 cut(s) 227
AjnI CCWGG 1 cut(s) 304
AluBI AGCT 1 cut(s) 437
AluI AGCT 1 cut(s) 437
AlwI GGATC 3 cut(s) 3, 16, 101
ApeKI GCWGC 1 cut(s) 535
ApoI RAATTY 2 cut(s) 344, 375
AspLEI GCGC 1 cut(s) 234
BaeGI GKGCMC 1 cut(s) 386
BaeI ACNNNNGTAYC 2 cut(s) 300, 333
BamHI GGATCC 1 cut(s) 8
BanII GRGCYC 2 cut(s) 192, 549
BauI CACGAG 1 cut(s) 385
BbvI GCAGC 1 cut(s) 522
BccI CCATC 3 cut(s) 104, 243, 434
BciT130I CCWGG 1 cut(s) 306
BclI TGATCA 1 cut(s) 39
BfaI CTAG 1 cut(s) 243
BfmI CTRYAG 1 cut(s) 103
BfoI RGCGCY 1 cut(s) 235
BisI GCNGC 1 cut(s) 536
BlsI GCNGC 1 cut(s) 537
Bme1390I CCNGG 1 cut(s) 306
BmiI GGNNCC 3 cut(s) 10, 189, 563
BmrFI CCNGG 1 cut(s) 306
BmrI ACTGGG 1 cut(s) 84
BmsI GCATC 1 cut(s) 148
BmuI ACTGGG 1 cut(s) 84
BpmI CTGGAG 1 cut(s) 189
BpuEI CTTGAG 1 cut(s) 256
BsaHI GRCGYC 1 cut(s) 571
BsaJI CCNNGG 1 cut(s) 183
Bsc4I CCNNNNNNNGG 1 cut(s) 18
Bse1I ACTGG 2 cut(s) 79, 206
BseBI CCWGG 1 cut(s) 306
BseDI CCNNGG 1 cut(s) 183
BseGI GGATG 3 cut(s) 115, 334, 424
BseLI CCNNNNNNNGG 1 cut(s) 18
BseNI ACTGG 2 cut(s) 79, 206
BseSI GKGCMC 1 cut(s) 386
BseXI GCAGC 1 cut(s) 522
BslFI GGGAC 2 cut(s) 34, 494
BslI CCNNNNNNNGG 1 cut(s) 18
BsmFI GGGAC 2 cut(s) 34, 494
Bsp1286I GDGCHC 3 cut(s) 192, 386, 549
Bsp1407I TGTACA 1 cut(s) 488
Bsp143I GATC 5 cut(s) 8, 39, 68, 93, 275
BspACI CCGC 1 cut(s) 581
BspLI GGNNCC 3 cut(s) 10, 189, 563
BspPI GGATC 3 cut(s) 3, 16, 101
BsrGI TGTACA 1 cut(s) 488
BsrI ACTGG 2 cut(s) 79, 206
BssECI CCNNGG 1 cut(s) 183
BssMI GATC 5 cut(s) 8, 39, 68, 93, 275
BssNI GRCGYC 1 cut(s) 571
BssSI CACGAG 1 cut(s) 385
BssT1I CCWWGG 1 cut(s) 183
Bst2BI CACGAG 1 cut(s) 385
Bst2UI CCWGG 1 cut(s) 306
Bst4CI ACNGT 2 cut(s) 129, 313
BstACI GRCGYC 1 cut(s) 571
BstAPI GCANNNNNTGC 2 cut(s) 523, 532
BstAUI TGTACA 1 cut(s) 488
BstF5I GGATG 3 cut(s) 115, 334, 424
BstH2I RGCGCY 1 cut(s) 235
BstHHI GCGC 1 cut(s) 234
BstKTI GATC 5 cut(s) 11, 42, 71, 96, 278
BstMBI GATC 5 cut(s) 8, 39, 68, 93, 275
BstMWI GCNNNNNNNGC 3 cut(s) 523, 532, 544
BstNI CCWGG 1 cut(s) 306
BstNSI RCATGY 1 cut(s) 177
BstSCI CCNGG 1 cut(s) 304
BstSFI CTRYAG 1 cut(s) 103
BstSLI GKGCMC 1 cut(s) 386
BstV1I GCAGC 1 cut(s) 522
BstX2I RGATCY 1 cut(s) 8
BstYI RGATCY 1 cut(s) 8
BtsCI GGATG 3 cut(s) 115, 334, 424
BtsIMutI CAGTG 2 cut(s) 124, 213
CfoI GCGC 1 cut(s) 234
CsiI ACCWGGT 1 cut(s) 304
Csp6I GTAC 2 cut(s) 309, 489
CviAII CATG 4 cut(s) 37, 174, 368, 407
CviJI RGCY 6 cut(s) 190, 202, 366, 398, 437, 547
CviKI_1 RGCY 6 cut(s) 190, 202, 366, 398, 437, 547
CviQI GTAC 2 cut(s) 309, 489
DpnI GATC 5 cut(s) 10, 41, 70, 95, 277
DpnII GATC 5 cut(s) 8, 39, 68, 93, 275
Eco130I CCWWGG 1 cut(s) 183
Eco24I GRGCYC 2 cut(s) 192, 549
EcoRI GAATTC 1 cut(s) 375
EcoRII CCWGG 1 cut(s) 304
EcoT14I CCWWGG 1 cut(s) 183
EcoT38I GRGCYC 2 cut(s) 192, 549
ErhI CCWWGG 1 cut(s) 183
FaeI CATG 4 cut(s) 40, 177, 371, 410
FaqI GGGAC 2 cut(s) 34, 494
FatI CATG 4 cut(s) 36, 173, 367, 406
FauI CCCGC 1 cut(s) 574
FauNDI CATATG 1 cut(s) 478
FbaI TGATCA 1 cut(s) 39
FblI GTMKAC 1 cut(s) 318
Fnu4HI GCNGC 1 cut(s) 536
FokI GGATG 3 cut(s) 122, 321, 431
FriOI GRGCYC 2 cut(s) 192, 549
Fsp4HI GCNGC 1 cut(s) 536
FspBI CTAG 1 cut(s) 243
GlaI GCGC 1 cut(s) 233
GluI GCNGC 1 cut(s) 536
GsuI CTGGAG 1 cut(s) 189
HaeII RGCGCY 1 cut(s) 235
HhaI GCGC 1 cut(s) 234
Hin1I GRCGYC 1 cut(s) 571
Hin1II CATG 4 cut(s) 40, 177, 371, 410
Hin6I GCGC 1 cut(s) 232
HinP1I GCGC 1 cut(s) 232
HincII GTYRAC 1 cut(s) 283
HindII GTYRAC 1 cut(s) 283
HinfI GANTC 3 cut(s) 269, 320, 388
Hpy166II GTNNAC 4 cut(s) 283, 319, 489, 491
Hpy188I TCNGA 1 cut(s) 576
Hpy188III TCNNGA 1 cut(s) 273
Hpy8I GTNNAC 4 cut(s) 283, 319, 489, 491
HpyAV CCTTC 2 cut(s) 116, 571
HpyCH4III ACNGT 2 cut(s) 129, 313
HpyCH4IV ACGT 2 cut(s) 493, 571
HpyCH4V TGCA 4 cut(s) 448, 517, 526, 538
HpyF10VI GCNNNNNNNGC 3 cut(s) 523, 532, 544
HpySE526I ACGT 2 cut(s) 493, 571
Hsp92I GRCGYC 1 cut(s) 571
Hsp92II CATG 4 cut(s) 40, 177, 371, 410
HspAI GCGC 1 cut(s) 232
Ksp22I TGATCA 1 cut(s) 39
Kzo9I GATC 5 cut(s) 8, 39, 68, 93, 275
LmnI GCTCC 1 cut(s) 187
Lsp1109I GCAGC 1 cut(s) 522
LweI GCATC 1 cut(s) 148
MabI ACCWGGT 1 cut(s) 304
MaeI CTAG 1 cut(s) 243
MaeII ACGT 2 cut(s) 493, 571
MaeIII GTNAC 1 cut(s) 208
MalI GATC 5 cut(s) 10, 41, 70, 95, 277
MboI GATC 5 cut(s) 8, 39, 68, 93, 275
MflI RGATCY 1 cut(s) 8
MhlI GDGCHC 3 cut(s) 192, 386, 549
MluCI AATT 2 cut(s) 344, 375
MlyI GAGTC 3 cut(s) 263, 314, 397
MmeI TCCRAC 1 cut(s) 362
MnlI CCTC 4 cut(s) 190, 213, 464, 477
MslI CAYNNNNRTG 1 cut(s) 405
MspA1I CMGCKG 1 cut(s) 437
MspR9I CCNGG 1 cut(s) 306
MvaI CCWGG 1 cut(s) 306
MwoI GCNNNNNNNGC 3 cut(s) 523, 532, 544
NdeI CATATG 1 cut(s) 478
NdeII GATC 5 cut(s) 8, 39, 68, 93, 275
NlaIII CATG 4 cut(s) 40, 177, 371, 410
NlaIV GGNNCC 3 cut(s) 10, 189, 563
NmuCI GTSAC 1 cut(s) 208
NspI RCATGY 1 cut(s) 177
PflFI GACNNNGTC 1 cut(s) 25
PkrI GCNGC 1 cut(s) 537
PleI GAGTC 3 cut(s) 263, 314, 396
PpsI GAGTC 3 cut(s) 263, 314, 396
Psp6I CCWGG 1 cut(s) 304
PspGI CCWGG 1 cut(s) 304
PspN4I GGNNCC 3 cut(s) 10, 189, 563
PsuI RGATCY 1 cut(s) 8
PsyI GACNNNGTC 1 cut(s) 25
PvuII CAGCTG 1 cut(s) 437
RsaI GTAC 2 cut(s) 310, 490
RsaNI GTAC 2 cut(s) 309, 489
RseI CAYNNNNRTG 1 cut(s) 405
SatI GCNGC 1 cut(s) 536
Sau3AI GATC 5 cut(s) 8, 39, 68, 93, 275
SchI GAGTC 3 cut(s) 263, 314, 397
ScrFI CCNGG 1 cut(s) 306
SduI GDGCHC 3 cut(s) 192, 386, 549
SetI ASST 8 cut(s) 248, 307, 360, 439, 468, 488, 496, 574
SexAI ACCWGGT 1 cut(s) 304
SfaNI GCATC 1 cut(s) 148
SfcI CTRYAG 1 cut(s) 103
SmiMI CAYNNNNRTG 1 cut(s) 405
SmlI CTYRAG 1 cut(s) 271
SmoI CTYRAG 1 cut(s) 271
Sse9I AATT 2 cut(s) 344, 375
SsiI CCGC 1 cut(s) 581
SspMI CTAG 1 cut(s) 243
StyD4I CCNGG 1 cut(s) 304
StyI CCWWGG 1 cut(s) 183
TaaI ACNGT 2 cut(s) 129, 313
TaiI ACGT 2 cut(s) 496, 574
TaqI TCGA 1 cut(s) 543
TasI AATT 2 cut(s) 344, 375
TatI WGTACW 1 cut(s) 488
TscAI CASTG 2 cut(s) 124, 213
TseFI GTSAC 1 cut(s) 208
TseI GCWGC 1 cut(s) 535
Tsp45I GTSAC 1 cut(s) 208
TspDTI ATGAA 1 cut(s) 165
TspGWI ACGGA 2 cut(s) 53, 106
TspRI CASTG 2 cut(s) 124, 213
Tth111I GACNNNGTC 1 cut(s) 25
XapI RAATTY 2 cut(s) 344, 375
XceI RCATGY 1 cut(s) 177
XmiI GTMKAC 1 cut(s) 318
XspI CTAG 1 cut(s) 243
ZraI GACGTC 1 cut(s) 572
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.