Rh7DG295300

Belongs to the argonaute family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0001348
Physical Location & Seq
Reverse (-)
33558541 .. 33575008
16468 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG295300.1

Sequence Viewer

Length: 3891 bp
ATGAAGATGCCAAAGCACGCAGAAGCAAGCTCTGTCTTTGTAGTGGTGACTCATTTTGAGGGCACCTGCTTTGGCGCATTATTTGAAGTAAAATTAGATGGACAAGGTAAAGAAGGTTCAGCAACTCATGAAACCCAACCAGTACTTGAGACTGTATCACCATTCACTACAAAGGATTTAGGTCGGGGAATGTGGAAATTTTGTACCTCGGGTGCCAAGTTATATATTTTGCCAGATGGTGGTCCTGATTTGTTTGACATGGAAGTGTGTTTCCCCACATGCAGTGGACATATTTTCGACATCAAGACTGGGAAATTGGTGTCATCATTCAAACCCCCAGCTCCCAAATGGTTTGGAACTCATGTGTCTGCATATGGGAAAATTTACTCTCTTTCAGATCCATATTGCTCATGTGATGTACCAGAGCCGTCTTTCGAGCAGTATGACCCTATCTCTCGGTCTTGGCAGAAGTTGTGCTCTTTTCCATATTCTGAGGAATACGGTAACACAGAGATACAGGGTTATGCTGTTTGTGATGAAATCTTATTGTTTAGTATATGTGGCTGTAAGGAAAACCGTGATGCAGTGTGGGCTTATGATGTAATTAAAAATAAGTGGTATCCAGTCCAGGTTGAGAATATAGGTTGCTATTTCCGAAGAAGGGCCGTGGTTGTGGAGAAGACTATCTATGCCTTATCAATATTGCACATGGTTGTTGTGGCATTCTCTATTACGAGCAACCAAAATGAGGAAGGGCATATTAGCTATTCAATAGGCTCATTGTTACAGTTAGATTTTCCACAGGTTATAGACTGGAGAAGTGATCAGCATTTGGTTCATTTGGGTCACCTTGTCTTTTATCTTGTCCAGAGTGGCTTCGATGACGAAGTTGATGTTCGCCAACCTCTTTCGATCACCAAATTTGAGATTGTGGGAGGAAGCGAAATCAATATCATAAGTTCAATGCTTCTTGAGGTGGATCTCGGGGATTCTGGGCCATTCCGTGTTCAATTATGCTTCAGGCCAGAGTGGGAGGATGTCACACCCATAGAAGAAGAGACTGCTACCTCCATCAACCTTTCAGTGCTGCACCAAGTAATTCCAGTTCCTTACCAAGCTGGAATCACCCCTCTGCCTGCATATGAAGTATGTTCCTCATCTCAGCTGCCACCTGAGCCTTCAGAAGTGGCAGAGCAGTTTCAGCAGGTTTCCATCCAGCAAGAGGAAGCTCCTAGTCAGTCCATTCAACAAGTGTCAGTCTCTACCAAGTCTATGATGTTCCCTCTTCGCCCTGGTAAGGGTACGACTGGCAAAAGGTTTCTAGTCAAGGCAAACCATTTCTTTGCTGAATTAACAGACAAGGATCTGCACCAGTATGATGTTACTATTACACCAGAAGTCACATCCCACAGTGTAAACCGTGCTGTGATGTTTCAGCTAGTGAAACTCTACAGGGAGTCCCATCTTTCAAAGCTGCTTCCTGTTTATGATGGGCGGAAGAGCCTGTATACTGCTGGGCCTTTTCCTTTCCAGTCAAAGGAGTTTAAAATCACTCTTCTGGATGATGATGATGAGGGTCGACAGGGACTCCCATCTTTCAAACCGGAGAGGGAGTTCAGAGTTGTGATCAAATTTGCTGCACGTGTTGACCTGCATGATTTAGTTCTCTTTTTGCAAGGGAGGAAAGCTGATGCACCCGAGAAGGCCCTTCAGGTTCTGAACATTGTTCTGAGAGAGTTGCCTACTTCTAGGTACTGTCCTGTGGGTCAGTCATTTTATGACCCTGGCTTGGGGAGAAAACTGCCACTTGGTGACGATTTAGAATACTGGCGTGGTTTTTACCAGAGTTTGCGTCCAACTCAAATGGGGCTATCTTTAAACATTAATATGTCATCGAGAGCCTTTATTGAGCCATTGCCAGTTATAGAGTTTGTAACCAAGCTTCTGAATCGCAATATTCAAGCTAAGCCACTTAGTCATTCTGACCGTGTAAAGATTGAAAAGGCTCTACGTGGGGTGATGGTTGAAGTTACACACCGCAAAAATATGCCCAGAAAATACTGTATTTCTGGTTTAACATCCCAGAAAACAAGAGAATTGACTTTTCCTGTTGGTGAAAGAGGAACAATGAAATCTGTTGTTGAATACTTTCTTGAAACCTACAGTTTTGCCATTCAATATCCATATTGGCCTTGTCTTCAAGTTGGAAATCAAGAACGACCGAACTATTTACCCATGGAGGTCTGTAAAATTGTTAAGGGACAAAGGTACTCAAATAGATTGAACATGATACAGACAATTGCATTGCTCAAGGAGACTATTCAGCGCCCTTTGGAAAAGGAACGTGATATTGTACAGATAGTACGTCATAATGCCTTCGATCAAGATCCTTATGCAAAGGAGTTCGGAATTAAAATTAGTGAGAATATGGCTCAGGTTGAAGCTCGTATTCTTCCTGCACCACGGCTTAAATACCATAATACTGGAAAGCAAAAGAATTGCCTGCCCAGCCTTGGACAGTGGAGCATGATAAATAAGAAAATGGTAAATGGAGGAAAAGTAAGCAATTGGATCTGCATAAATTTTTCCCATAATGTACAAGAGAGTGTGGCAAAGAGTTTTTGTTATGAACTTGCTCAGATGTGTCACATTTATGGCATGGTGTTCAATGCAGAACCTGTGCTTCCACTAGTCAGTGCTCGACCTGATCAGGTGGAGAAGGTCTTAAAAACTCAGTATCATGATGTGATGACTAGGATTCCAGGCAAGGAGCTTGACTTACTTGTTGTCATCTTGCCAAATAATAACGGCTCTCTTTATGGTGATTTAAAAAGGATTTGTGAGACGGAGCTTGGCCTGGTATCCCAGTGCTGTTTAACTAAACATGTGTTTAAGATGCATAAACAGTATTTAGCAAACATTGCCTTGAAGATAAATGTGAAGGTCGGGGGACGGAACACAGTACTTATTGATGCAATCTCCAGACGTATTCCCTTGGTTAGTGACCGTCCTACAATAATTTTCGGTGCTGCTATGACGCATCCTCATCCAGGGGAGGATTCAAGCCCATCAATTGCAGCTGTTGTAGCTTCTCAAGACTGGCCTGAAATTACCATGTATGCTGGTTTAGTTTGTGCTCAAGCTTATCGTCAGGAGCTCATCCAAGATTTGTTTAAACAGTGGCACGATCCGGTAGAAGGGGCAATGTCTGGTGGAATGATAAAGGAACTGCTTATTTCATTTTATGGAGCGACAGGGCAGAAGCCTCAACGCATCATTTTCTACAGGAAAAGTGTCAGCGAGGGGCAGTTCTACCAAGTTCTACTGTACGAACTTGATGCAATTCGTAAGGCCTGCGCTTCACTGGAGCCAAATTATCAGCCTCCTGTGACTTTTGTTGTTGTTCAAAAGAGTCATCGTACAAGACTGTTTGCCAACAACCATGGTGATCGCCGTGCAGTTGACGGGAGTGGGAACATATTACCTGGTACGGTTGTGGACTCCAAAATCTGTCACCCAACTGAGTTTGACTTCTACCTCTGTAGCCATGCAGGAATTCAGGGTACAAGCCTCCCAGCTCATTACCATGTGTTGTGGGATGAAAATAAGTTCACAGCAGACGCACTGCAGTCCCTTACCAATAATCTTTGCTATACATATGCAAGGTGTACACGCTCCGTTTCAATTGTGCCTCCTGCTTATTATGCTCATCTCGCCGCAATCCGAGCTCGGTTCTATCTGGAGCCCGTGACTTCAGACAGTGGTTCAATGTCAAGTGTTATGGGTGGTTCTGGTGGAGGACGGAACTCTCGAGCACCGGGTCCAAAAGCTGCTGTGAAGGTGTTGCCTGCGTTGAAGGAGAATGTGAAAAAGGTCATGTTCTACTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ArgoL1 ArgoL2 ArgoMid ArgoN Gly-rich_Ago1 PAZ Piwi

Protein Analysis

1296

Amino Acids

145.9

Weight (kDa)

7.81

Isoelectric Point (pI)

47.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gly-rich_Ago1 PF12764 363 - 418 6e-07 Glycine-rich region of argonaut
ArgoN PF16486 439 - 577 2.7e-32 N-terminal domain of argonaute
ArgoL1 PF08699 587 - 637 4.4e-17 Argonaute linker 1 domain
PAZ PF02170 650 - 768 2.1e-27 PAZ domain
ArgoL2 PF16488 780 - 825 3.7e-17 Argonaute linker 2 domain
ArgoMid PF16487 836 - 911 6.2e-08 Mid domain of argonaute
Piwi PF02171 928 - 1246 1.6e-106 Piwi domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 74
Acc36I ACCTGC 3 cut(s) 74, 1195, 1659
AccB1I GGYRCC 2 cut(s) 62, 212
AccB7I CCANNNNNTGG 1 cut(s) 239
AccI GTMKAC 2 cut(s) 1508, 1579
AciI CCGC 3 cut(s) 1495, 2038, 3717
AclWI GGATC 6 cut(s) 392, 987, 1371, 2381, 2579, 3182
AcsI RAATTY 6 cut(s) 197, 381, 920, 1631, 2581, 3555
AcuI CTGAAG 4 cut(s) 1003, 1164, 1694, 3738
AcvI CACGTG 1 cut(s) 1643
AdeI CACNNNGTG 1 cut(s) 1811
AflIII ACRYGT 2 cut(s) 1642, 2884
AhlI ACTAGT 1 cut(s) 2689
AjnI CCWGG 7 cut(s) 627, 1291, 1783, 2761, 2856, 3049, 3484
AloI GAACNNNNNNTCC 2 cut(s) 1598, 1630
Alw21I GWGCWC 6 cut(s) 479, 2701, 3139, 3159, 3730, 3817
Alw26I GTCTC 5 cut(s) 143, 1052, 1264, 2309, 2837
AlwI GGATC 6 cut(s) 392, 987, 1371, 2381, 2579, 3182
AlwNI CAGNNNCTG 2 cut(s) 1717, 2678
Ama87I CYCGRG 4 cut(s) 208, 983, 1697, 3810
AoxI GGCC 9 cut(s) 663, 995, 1022, 1517, 1704, 2189, 2854, 3101, 3351
ApeKI GCWGC 7 cut(s) 1087, 1165, 1474, 1637, 3029, 3077, 3830
ApoI RAATTY 6 cut(s) 197, 381, 920, 1631, 2581, 3555
AseI ATTAAT 1 cut(s) 1884
Asp700I GAANNNNTTC 1 cut(s) 2148
AspLEI GCGC 3 cut(s) 77, 2328, 3359
AspS9I GGNCC 6 cut(s) 242, 663, 995, 1517, 1705, 3821
AsuC2I CCSGG 1 cut(s) 3819
AvaI CYCGRG 4 cut(s) 208, 983, 1697, 3810
AvaII GGWCC 2 cut(s) 242, 3821
BaeGI GKGCMC 1 cut(s) 65
BaeI ACNNNNGTAYC 2 cut(s) 3480, 3513
BanI GGYRCC 2 cut(s) 62, 212
BanII GRGCYC 3 cut(s) 3159, 3730, 3747
BarI GAAGNNNNNNTAC 2 cut(s) 1926, 1958
BbrPI CACGTG 1 cut(s) 1643
BbsI GAAGAC 2 cut(s) 686, 2189
Bbv12I GWGCWC 6 cut(s) 479, 2701, 3139, 3159, 3730, 3817
BbvI GCAGC 7 cut(s) 1074, 1152, 1461, 1624, 3016, 3089, 3817
BccI CCATC 9 cut(s) 92, 230, 1079, 1220, 1470, 1484, 1600, 2014, 3076
BceAI ACGGC 5 cut(s) 412, 650, 2480, 2824, 3438
BcgI CGANNNNNNTGC 2 cut(s) 3320, 3354
BciT130I CCWGG 7 cut(s) 629, 1293, 1785, 2763, 2858, 3051, 3486
BciVI GTATCC 2 cut(s) 630, 2872
BclI TGATCA 3 cut(s) 823, 1626, 2707
BcnI CCSGG 1 cut(s) 3819
BcoDI GTCTC 5 cut(s) 143, 1052, 1264, 2309, 2837
BcuI ACTAGT 1 cut(s) 2689
BfaI CTAG 7 cut(s) 1233, 1322, 1439, 1749, 2690, 2754, 3889
BfmI CTRYAG 5 cut(s) 1450, 2161, 3283, 3541, 3626
BfoI RGCGCY 1 cut(s) 2329
BfuAI ACCTGC 3 cut(s) 74, 1195, 1659
BfuI GTATCC 2 cut(s) 630, 2872
BisI GCNGC 8 cut(s) 1088, 1166, 1475, 1638, 3030, 3078, 3717, 3831
BlpI GCTNAGC 1 cut(s) 1965
BlsI GCNGC 8 cut(s) 1089, 1167, 1476, 1639, 3031, 3079, 3718, 3832
BmcAI AGTACT 2 cut(s) 144, 2964
Bme1390I CCNGG 8 cut(s) 629, 1293, 1785, 2763, 2858, 3051, 3486, 3819
Bme18I GGWCC 2 cut(s) 242, 3821
BmeT110I CYCGRG 4 cut(s) 208, 983, 1697, 3810
BmgT120I GGNCC 6 cut(s) 242, 663, 995, 1517, 1705, 3821
BmiI GGNNCC 5 cut(s) 64, 214, 3369, 3744, 3822
BmrFI CCNGG 8 cut(s) 629, 1293, 1785, 2763, 2858, 3051, 3486, 3819
BmrI ACTGGG 2 cut(s) 318, 2860
BmsI GCATC 7 cut(s) 571, 1681, 2886, 2962, 3049, 3282, 3328
BmuI ACTGGG 2 cut(s) 318, 2860
BpiI GAAGAC 2 cut(s) 686, 2189
BpmI CTGGAG 4 cut(s) 835, 2965, 3386, 3761
Bpu10I CCTNAGC 2 cut(s) 1173, 2433
Bpu1102I GCTNAGC 1 cut(s) 1965
BpuEI CTTGAG 5 cut(s) 167, 992, 2294, 3078, 3123
BpuMI CCSGG 1 cut(s) 3819
BsaAI YACGTR 2 cut(s) 1643, 2012
BsaBI GATNNNNATC 1 cut(s) 2385
BsaWI WCCGGW 2 cut(s) 1603, 3190
BsaXI ACNNNNNCTCC 6 cut(s) 1572, 1598, 1602, 1628, 2544, 2574
Bse3DI GCAATG 4 cut(s) 1913, 2303, 2919, 3210
Bse8I GATNNNNATC 1 cut(s) 2385
BseBI CCWGG 7 cut(s) 629, 1293, 1785, 2763, 2858, 3051, 3486
BseGI GGATG 9 cut(s) 1042, 1212, 1403, 1567, 2078, 3040, 3046, 3159, 3604
BseJI GATNNNNATC 1 cut(s) 2385
BseMI GCAATG 4 cut(s) 1913, 2303, 2919, 3210
BseMII CTCAG 8 cut(s) 483, 1164, 1175, 1721, 2447, 2651, 2747, 3513
BseSI GKGCMC 1 cut(s) 65
BseXI GCAGC 7 cut(s) 1074, 1152, 1461, 1624, 3016, 3089, 3817
BseYI CCCAGC 4 cut(s) 337, 1514, 2507, 3574
BsgI GTGCAG 5 cut(s) 1073, 1352, 1623, 2442, 3477
Bsh1285I CGRYCG 1 cut(s) 2222
BshFI GGCC 9 cut(s) 665, 997, 1024, 1519, 1706, 2191, 2856, 3103, 3353
BshNI GGYRCC 2 cut(s) 62, 212
BsiEI CGRYCG 1 cut(s) 2222
BsiHKAI GWGCWC 6 cut(s) 479, 2701, 3139, 3159, 3730, 3817
BsiHKCI CYCGRG 4 cut(s) 208, 983, 1697, 3810
BsiSI CCGG 3 cut(s) 1604, 3191, 3818
BslFI GGGAC 5 cut(s) 1444, 1599, 2274, 2964, 3616
BsmAI GTCTC 5 cut(s) 143, 1052, 1264, 2309, 2837
BsmBI CGTCTC 1 cut(s) 2837
BsmFI GGGAC 5 cut(s) 1444, 1599, 2274, 2964, 3616
BsmI GAATGC 1 cut(s) 722
BsnI GGCC 9 cut(s) 665, 997, 1024, 1519, 1706, 2191, 2856, 3103, 3353
BsoBI CYCGRG 4 cut(s) 208, 983, 1697, 3810
Bsp1286I GDGCHC 8 cut(s) 65, 479, 2701, 3139, 3159, 3730, 3747, 3817
Bsp1407I TGTACA 3 cut(s) 2353, 2596, 3668
Bsp1720I GCTNAGC 1 cut(s) 1965
Bsp19I CCATGG 2 cut(s) 2235, 3442
BspACI CCGC 3 cut(s) 1495, 2038, 3717
BspANI GGCC 9 cut(s) 665, 997, 1024, 1519, 1706, 2191, 2856, 3103, 3353
BspCNI CTCAG 8 cut(s) 484, 1165, 1174, 1722, 2446, 2650, 2746, 3514
BspHI TCATGA 2 cut(s) 127, 2740
BspLI GGNNCC 5 cut(s) 64, 214, 3369, 3744, 3822
BspMAI CTGCAG 1 cut(s) 3630
BspMI ACCTGC 3 cut(s) 74, 1195, 1659
BspPI GGATC 6 cut(s) 392, 987, 1371, 2381, 2579, 3182
BspQI GCTCTTC 1 cut(s) 1493
BspT107I GGYRCC 2 cut(s) 62, 212
BsrDI GCAATG 4 cut(s) 1913, 2303, 2919, 3210
BsrGI TGTACA 3 cut(s) 2353, 2596, 3668
BssNAI GTATAC 1 cut(s) 1509
BssT1I CCWWGG 4 cut(s) 2235, 2512, 2994, 3442
Bst1107I GTATAC 1 cut(s) 1509
Bst2UI CCWGG 7 cut(s) 629, 1293, 1785, 2763, 2858, 3051, 3486
Bst6I CTCTTC 4 cut(s) 1050, 1290, 1493, 1560
BstAPI GCANNNNNTGC 1 cut(s) 2921
BstAUI TGTACA 3 cut(s) 2353, 2596, 3668
BstBAI YACGTR 2 cut(s) 1643, 2012
BstC8I GCNNGC 6 cut(s) 18, 28, 1137, 2504, 3355, 3849
BstDSI CCRYGG 4 cut(s) 666, 2235, 2462, 3442
BstEII GGTNACC 1 cut(s) 845
BstENI CCTNNNNNAGG 1 cut(s) 3048
BstF5I GGATG 9 cut(s) 1042, 1212, 1403, 1567, 2078, 3040, 3046, 3159, 3604
BstH2I RGCGCY 1 cut(s) 2329
BstHHI GCGC 3 cut(s) 77, 2328, 3359
BstMAI GTCTC 5 cut(s) 143, 1052, 1264, 2309, 2837
BstMCI CGRYCG 1 cut(s) 2222
BstMWI GCNNNNNNNGC 9 cut(s) 590, 1174, 1201, 2508, 2921, 3086, 3704, 3713, 3725
BstNI CCWGG 7 cut(s) 629, 1293, 1785, 2763, 2858, 3051, 3486
BstNSI RCATGY 2 cut(s) 282, 2888
BstPI GGTNACC 1 cut(s) 845
BstSCI CCNGG 8 cut(s) 627, 1291, 1783, 2761, 2856, 3049, 3484, 3817
BstSFI CTRYAG 5 cut(s) 1450, 2161, 3283, 3541, 3626
BstSLI GKGCMC 1 cut(s) 65
BstV1I GCAGC 7 cut(s) 1074, 1152, 1461, 1624, 3016, 3089, 3817
BstV2I GAAGAC 2 cut(s) 686, 2189
BstX2I RGATCY 5 cut(s) 397, 979, 1363, 2386, 2571
BstXI CCANNNNNNTGG 1 cut(s) 2483
BstYI RGATCY 5 cut(s) 397, 979, 1363, 2386, 2571
BstZ17I GTATAC 1 cut(s) 1509
BsuI GTATCC 2 cut(s) 630, 2872
BsuRI GGCC 9 cut(s) 665, 997, 1024, 1519, 1706, 2191, 2856, 3103, 3353
BtgI CCRYGG 4 cut(s) 666, 2235, 2462, 3442
BtsCI GGATG 9 cut(s) 1042, 1212, 1403, 1567, 2078, 3040, 3046, 3159, 3604
BtsI GCAGTG 3 cut(s) 289, 591, 3623
BveI ACCTGC 3 cut(s) 74, 1195, 1659
Cac8I GCNNGC 6 cut(s) 18, 28, 1137, 2504, 3355, 3849
CaiI CAGNNNCTG 2 cut(s) 1717, 2678
CciI TCATGA 2 cut(s) 127, 2740
CfoI GCGC 3 cut(s) 77, 2328, 3359
Cfr13I GGNCC 6 cut(s) 242, 663, 995, 1517, 1705, 3821
CseI GACGC 3 cut(s) 1841, 3046, 3629
CsiI ACCWGGT 1 cut(s) 3484
DraI TTTAAA 4 cut(s) 1546, 1878, 2829, 3175
DraIII CACNNNGTG 1 cut(s) 1811
Eam1104I CTCTTC 4 cut(s) 1050, 1290, 1493, 1560
EarI CTCTTC 4 cut(s) 1050, 1290, 1493, 1560
EciI GGCGGA 1 cut(s) 1510
Ecl136II GAGCTC 2 cut(s) 3157, 3728
Eco130I CCWWGG 4 cut(s) 2235, 2512, 2994, 3442
Eco147I AGGCCT 1 cut(s) 3353
Eco24I GRGCYC 3 cut(s) 3159, 3730, 3747
Eco47I GGWCC 2 cut(s) 242, 3821
Eco53kI GAGCTC 2 cut(s) 3157, 3728
Eco57I CTGAAG 4 cut(s) 1003, 1164, 1694, 3738
Eco72I CACGTG 1 cut(s) 1643
Eco88I CYCGRG 4 cut(s) 208, 983, 1697, 3810
Eco91I GGTNACC 1 cut(s) 845
EcoICRI GAGCTC 2 cut(s) 3157, 3728
EcoNI CCTNNNNNAGG 1 cut(s) 3048
EcoO109I RGGNCCY 1 cut(s) 1705
EcoO65I GGTNACC 1 cut(s) 845
EcoRI GAATTC 1 cut(s) 3555
EcoRII CCWGG 7 cut(s) 627, 1291, 1783, 2761, 2856, 3049, 3484
EcoT14I CCWWGG 4 cut(s) 2235, 2512, 2994, 3442
EcoT22I ATGCAT 1 cut(s) 2901
EcoT38I GRGCYC 3 cut(s) 3159, 3730, 3747
ErhI CCWWGG 4 cut(s) 2235, 2512, 2994, 3442
Esp3I CGTCTC 1 cut(s) 2837
FaqI GGGAC 5 cut(s) 1444, 1599, 2274, 2964, 3616
FauNDI CATATG 3 cut(s) 373, 1141, 3658
FbaI TGATCA 3 cut(s) 823, 1626, 2707
FblI GTMKAC 2 cut(s) 1508, 1579
Fnu4HI GCNGC 8 cut(s) 1088, 1166, 1475, 1638, 3030, 3078, 3717, 3831
FokI GGATG 9 cut(s) 1049, 1199, 1390, 1574, 2065, 3027, 3033, 3146, 3611
FriOI GRGCYC 3 cut(s) 3159, 3730, 3747
Fsp4HI GCNGC 8 cut(s) 1088, 1166, 1475, 1638, 3030, 3078, 3717, 3831
FspBI CTAG 7 cut(s) 1233, 1322, 1439, 1749, 2690, 2754, 3889
GlaI GCGC 3 cut(s) 76, 2327, 3358
GluI GCNGC 8 cut(s) 1088, 1166, 1475, 1638, 3030, 3078, 3717, 3831
GsaI CCCAGC 4 cut(s) 341, 1518, 2511, 3578
GsuI CTGGAG 4 cut(s) 835, 2965, 3386, 3761
HaeII RGCGCY 1 cut(s) 2329
HaeIII GGCC 9 cut(s) 665, 997, 1024, 1519, 1706, 2191, 2856, 3103, 3353
HapII CCGG 3 cut(s) 1604, 3191, 3818
HgaI GACGC 3 cut(s) 1841, 3046, 3629
HhaI GCGC 3 cut(s) 77, 2328, 3359
Hin6I GCGC 3 cut(s) 75, 2326, 3357
HinP1I GCGC 3 cut(s) 75, 2326, 3357
HincII GTYRAC 3 cut(s) 1580, 1648, 3463
HindII GTYRAC 3 cut(s) 1580, 1648, 3463
HindIII AAGCTT 2 cut(s) 1940, 3141
HpaII CCGG 3 cut(s) 1604, 3191, 3818
HpyCH4IV ACGT 5 cut(s) 1642, 2011, 2344, 2365, 2986
HpyF10VI GCNNNNNNNGC 9 cut(s) 590, 1174, 1201, 2508, 2921, 3086, 3704, 3713, 3725
HpySE526I ACGT 5 cut(s) 1642, 2011, 2344, 2365, 2986
HspAI GCGC 3 cut(s) 75, 2326, 3357
Ksp22I TGATCA 3 cut(s) 823, 1626, 2707
LguI GCTCTTC 1 cut(s) 1493
Lsp1109I GCAGC 7 cut(s) 1074, 1152, 1461, 1624, 3016, 3089, 3817
LweI GCATC 7 cut(s) 571, 1681, 2886, 2962, 3049, 3282, 3328
MabI ACCWGGT 1 cut(s) 3484
MaeI CTAG 7 cut(s) 1233, 1322, 1439, 1749, 2690, 2754, 3889
MaeII ACGT 5 cut(s) 1642, 2011, 2344, 2365, 2986
MfeI CAATTG 4 cut(s) 2298, 2566, 3072, 3684
MflI RGATCY 5 cut(s) 397, 979, 1363, 2386, 2571
MhlI GDGCHC 8 cut(s) 65, 479, 2701, 3139, 3159, 3730, 3747, 3817
MlyI GAGTC 5 cut(s) 43, 1466, 1581, 3421, 3494
MmeI TCCRAC 2 cut(s) 1880, 2185
Mph1103I ATGCAT 1 cut(s) 2901
MroXI GAANNNNTTC 1 cut(s) 2148
MslI CAYNNNNRTG 5 cut(s) 263, 1374, 1886, 2652, 3585
MspA1I CMGCKG 2 cut(s) 1165, 3080
MspI CCGG 3 cut(s) 1604, 3191, 3818
MspR9I CCNGG 8 cut(s) 629, 1293, 1785, 2763, 2858, 3051, 3486, 3819
MssI GTTTAAAC 1 cut(s) 3175
MunI CAATTG 4 cut(s) 2298, 2566, 3072, 3684
Mva1269I GAATGC 1 cut(s) 722
MvaI CCWGG 7 cut(s) 629, 1293, 1785, 2763, 2858, 3051, 3486
MwoI GCNNNNNNNGC 9 cut(s) 590, 1174, 1201, 2508, 2921, 3086, 3704, 3713, 3725
NciI CCSGG 1 cut(s) 3819
NcoI CCATGG 2 cut(s) 2235, 3442
NdeI CATATG 3 cut(s) 373, 1141, 3658
NlaIV GGNNCC 5 cut(s) 64, 214, 3369, 3744, 3822
NsiI ATGCAT 1 cut(s) 2901
NspI RCATGY 2 cut(s) 282, 2888
PaeR7I CTCGAG 1 cut(s) 3810
PagI TCATGA 2 cut(s) 127, 2740
PaqCI CACCTGC 1 cut(s) 74
PceI AGGCCT 1 cut(s) 3353
PciI ACATGT 1 cut(s) 2884
PciSI GCTCTTC 1 cut(s) 1493
PcsI WCGNNNNNNNCGW 1 cut(s) 3808
PctI GAATGC 1 cut(s) 722
PdmI GAANNNNTTC 1 cut(s) 2148
PfeI GAWTC 5 cut(s) 989, 1122, 1948, 2758, 3059
PflMI CCANNNNNTGG 1 cut(s) 239
PkrI GCNGC 8 cut(s) 1089, 1167, 1476, 1639, 3031, 3079, 3718, 3832
PleI GAGTC 5 cut(s) 43, 1465, 1581, 3420, 3494
PmaCI CACGTG 1 cut(s) 1643
PmeI GTTTAAAC 1 cut(s) 3175
PmlI CACGTG 1 cut(s) 1643
PpsI GAGTC 5 cut(s) 43, 1465, 1581, 3420, 3494
Ppu21I YACGTR 2 cut(s) 1643, 2012
PscI ACATGT 1 cut(s) 2884
PshBI ATTAAT 1 cut(s) 1884
Psp124BI GAGCTC 2 cut(s) 3159, 3730
Psp6I CCWGG 7 cut(s) 627, 1291, 1783, 2761, 2856, 3049, 3484
PspCI CACGTG 1 cut(s) 1643
PspEI GGTNACC 1 cut(s) 845
PspFI CCCAGC 4 cut(s) 337, 1514, 2507, 3574
PspGI CCWGG 7 cut(s) 627, 1291, 1783, 2761, 2856, 3049, 3484
PspN4I GGNNCC 5 cut(s) 64, 214, 3369, 3744, 3822
PspPI GGNCC 6 cut(s) 242, 663, 995, 1517, 1705, 3821
PsrI GAACNNNNNNTAC 4 cut(s) 100, 132, 1089, 1121
PstI CTGCAG 1 cut(s) 3630
PstNI CAGNNNCTG 2 cut(s) 1717, 2678
PsuI RGATCY 5 cut(s) 397, 979, 1363, 2386, 2571
PvuII CAGCTG 2 cut(s) 1165, 3080
RseI CAYNNNNRTG 5 cut(s) 263, 1374, 1886, 2652, 3585
SacI GAGCTC 2 cut(s) 3159, 3730
SalI GTCGAC 1 cut(s) 1578
SapI GCTCTTC 1 cut(s) 1493
SatI GCNGC 8 cut(s) 1088, 1166, 1475, 1638, 3030, 3078, 3717, 3831
Sau96I GGNCC 6 cut(s) 242, 663, 995, 1517, 1705, 3821
ScaI AGTACT 2 cut(s) 144, 2964
SchI GAGTC 5 cut(s) 43, 1466, 1581, 3421, 3494
ScrFI CCNGG 8 cut(s) 629, 1293, 1785, 2763, 2858, 3051, 3486, 3819
SduI GDGCHC 8 cut(s) 65, 479, 2701, 3139, 3159, 3730, 3747, 3817
SexAI ACCWGGT 1 cut(s) 3484
SfaNI GCATC 7 cut(s) 571, 1681, 2886, 2962, 3049, 3282, 3328
SfcI CTRYAG 5 cut(s) 1450, 2161, 3283, 3541, 3626
Sfr274I CTCGAG 1 cut(s) 3810
SinI GGWCC 2 cut(s) 242, 3821
SlaI CTCGAG 1 cut(s) 3810
SmiMI CAYNNNNRTG 5 cut(s) 263, 1374, 1886, 2652, 3585
SmlI CTYRAG 6 cut(s) 146, 971, 2309, 3093, 3138, 3810
SmoI CTYRAG 6 cut(s) 146, 971, 2309, 3093, 3138, 3810
SpeI ACTAGT 1 cut(s) 2689
SseBI AGGCCT 1 cut(s) 3353
SsiI CCGC 3 cut(s) 1495, 2038, 3717
SspI AATATT 2 cut(s) 702, 1957
SspMI CTAG 7 cut(s) 1233, 1322, 1439, 1749, 2690, 2754, 3889
SstI GAGCTC 2 cut(s) 3159, 3730
StuI AGGCCT 1 cut(s) 3353
StyD4I CCNGG 8 cut(s) 627, 1291, 1783, 2761, 2856, 3049, 3484, 3817
StyI CCWWGG 4 cut(s) 2235, 2512, 2994, 3442
TaiI ACGT 5 cut(s) 1645, 2014, 2347, 2368, 2989
TaqI TCGA 9 cut(s) 297, 435, 879, 911, 1579, 1895, 2379, 2701, 3811
TaqII GACCGA 2 cut(s) 447, 2236
TatI WGTACW 5 cut(s) 142, 2353, 2596, 2962, 3668
TauI GCSGC 1 cut(s) 3719
TfiI GAWTC 5 cut(s) 989, 1122, 1948, 2758, 3059
TseI GCWGC 7 cut(s) 1087, 1165, 1474, 1637, 3029, 3077, 3830
TspDTI ATGAA 9 cut(s) 17, 144, 552, 827, 1158, 2144, 2643, 3228, 3615
TspGWI ACGGA 5 cut(s) 992, 2861, 2968, 3667, 3817
Van91I CCANNNNNTGG 1 cut(s) 239
VpaK11BI GGWCC 2 cut(s) 242, 3821
VspI ATTAAT 1 cut(s) 1884
XagI CCTNNNNNAGG 1 cut(s) 3048
XapI RAATTY 6 cut(s) 197, 381, 920, 1631, 2581, 3555
XceI RCATGY 2 cut(s) 282, 2888
XcmI CCANNNNNNNNNTGG 1 cut(s) 345
XhoI CTCGAG 1 cut(s) 3810
XmiI GTMKAC 2 cut(s) 1508, 1579
XmnI GAANNNNTTC 1 cut(s) 2148
XspI CTAG 7 cut(s) 1233, 1322, 1439, 1749, 2690, 2754, 3889
ZrmI AGTACT 2 cut(s) 144, 2964
Zsp2I ATGCAT 1 cut(s) 2901
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.