subtilisin-like
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 4 cut(s) | 524, 923, 1602, 1657 |
| AatII | GACGTC | 4 cut(s) | 501, 1655, 2048, 2123 |
| AccB1I | GGYRCC | 2 cut(s) | 647, 2227 |
| AccI | GTMKAC | 3 cut(s) | 92, 1757, 2048 |
| AccIII | TCCGGA | 1 cut(s) | 1708 |
| AclWI | GGATC | 4 cut(s) | 1094, 1122, 1838, 1851 |
| AcoI | YGGCCR | 9 cut(s) | 81, 213, 291, 454, 489, 530, 733, 1422, 1443 |
| AcsI | RAATTY | 2 cut(s) | 958, 1513 |
| AcuI | CTGAAG | 1 cut(s) | 1715 |
| AcvI | CACGTG | 3 cut(s) | 594, 1672, 1840 |
| AfiI | CCNNNNNNNGG | 9 cut(s) | 528, 582, 694, 819, 823, 1254, 1348, 1452, 1864 |
| AflIII | ACRYGT | 2 cut(s) | 1624, 1837 |
| AgsI | TTSAA | 4 cut(s) | 616, 1636, 1921, 2236 |
| AhdI | GACNNNNNGTC | 2 cut(s) | 1281, 2131 |
| AjiI | CACGTC | 1 cut(s) | 1449 |
| AjnI | CCWGG | 6 cut(s) | 338, 581, 650, 805, 1555, 1947 |
| AleI | CACNNNNGTG | 2 cut(s) | 1094, 1674 |
| AluBI | AGCT | 9 cut(s) | 62, 70, 418, 546, 882, 995, 1065, 1364, 2307 |
| AluI | AGCT | 9 cut(s) | 62, 70, 418, 546, 882, 995, 1065, 1364, 2307 |
| Alw21I | GWGCWC | 2 cut(s) | 420, 1034 |
| Alw26I | GTCTC | 4 cut(s) | 10, 286, 461, 1615 |
| AlwI | GGATC | 4 cut(s) | 1094, 1122, 1838, 1851 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 50 |
| Ama87I | CYCGRG | 3 cut(s) | 437, 1503, 1864 |
| Aor13HI | TCCGGA | 1 cut(s) | 1708 |
| ApaI | GGGCCC | 3 cut(s) | 689, 1052, 1511 |
| ApeKI | GCWGC | 6 cut(s) | 62, 143, 359, 1364, 1690, 1944 |
| ApoI | RAATTY | 2 cut(s) | 958, 1513 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 545, 577 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 2246 |
| AspLEI | GCGC | 9 cut(s) | 650, 723, 785, 805, 1208, 1690, 1705, 1741, 2230 |
| AsuC2I | CCSGG | 8 cut(s) | 381, 780, 1255, 1648, 1721, 1801, 1865, 1866 |
| AsuHPI | GGTGA | 5 cut(s) | 506, 2035, 2089, 2149, 2191 |
| AvaI | CYCGRG | 3 cut(s) | 437, 1503, 1864 |
| AvaII | GGWCC | 4 cut(s) | 432, 566, 1595, 1999 |
| BaeGI | GKGCMC | 4 cut(s) | 576, 689, 1052, 1511 |
| BalI | TGGCCA | 2 cut(s) | 83, 215 |
| BamHI | GGATCC | 1 cut(s) | 1843 |
| BanI | GGYRCC | 2 cut(s) | 647, 2227 |
| BanII | GRGCYC | 6 cut(s) | 420, 689, 1052, 1511, 1721, 2157 |
| BauI | CACGAG | 2 cut(s) | 1027, 2068 |
| BbrPI | CACGTG | 3 cut(s) | 594, 1672, 1840 |
| Bbv12I | GWGCWC | 2 cut(s) | 420, 1034 |
| BbvI | GCAGC | 6 cut(s) | 49, 155, 371, 1351, 1677, 1956 |
| BccI | CCATC | 8 cut(s) | 329, 698, 817, 982, 1000, 1264, 1483, 1816 |
| BciT130I | CCWGG | 6 cut(s) | 340, 583, 652, 807, 1557, 1949 |
| BcnI | CCSGG | 8 cut(s) | 381, 780, 1255, 1648, 1721, 1801, 1865, 1866 |
| BcoDI | GTCTC | 4 cut(s) | 10, 286, 461, 1615 |
| BfaI | CTAG | 2 cut(s) | 1335, 1571 |
| BfmI | CTRYAG | 1 cut(s) | 141 |
| BfoI | RGCGCY | 5 cut(s) | 651, 1209, 1691, 1742, 2231 |
| BlpI | GCTNAGC | 1 cut(s) | 66 |
| BmcAI | AGTACT | 2 cut(s) | 387, 513 |
| Bme18I | GGWCC | 4 cut(s) | 432, 566, 1595, 1999 |
| BmeRI | GACNNNNNGTC | 2 cut(s) | 1281, 2131 |
| BmeT110I | CYCGRG | 3 cut(s) | 437, 1503, 1864 |
| BmgBI | CACGTC | 1 cut(s) | 1449 |
| BmrI | ACTGGG | 2 cut(s) | 472, 1047 |
| BmsI | GCATC | 3 cut(s) | 253, 1606, 1714 |
| BmuI | ACTGGG | 2 cut(s) | 472, 1047 |
| BoxI | GACNNNNGTC | 1 cut(s) | 1460 |
| BpmI | CTGGAG | 3 cut(s) | 1464, 1539, 2279 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 66 |
| BpuEI | CTTGAG | 1 cut(s) | 392 |
| BpuMI | CCSGG | 8 cut(s) | 381, 780, 1255, 1648, 1721, 1801, 1865, 1866 |
| Bsa29I | ATCGAT | 1 cut(s) | 1614 |
| BsaAI | YACGTR | 3 cut(s) | 594, 1672, 1840 |
| BsaI | GGTCTC | 1 cut(s) | 10 |
| BsaWI | WCCGGW | 4 cut(s) | 557, 1163, 1708, 2219 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 317, 347, 602, 632 |
| Bsc4I | CCNNNNNNNGG | 9 cut(s) | 528, 582, 694, 819, 823, 1254, 1348, 1452, 1864 |
| Bse118I | RCCGGY | 5 cut(s) | 491, 521, 1229, 1441, 2092 |
| Bse1I | ACTGG | 7 cut(s) | 260, 478, 519, 1053, 1116, 1422, 1481 |
| Bse3DI | GCAATG | 1 cut(s) | 2307 |
| BseAI | TCCGGA | 1 cut(s) | 1708 |
| BseBI | CCWGG | 6 cut(s) | 340, 583, 652, 807, 1557, 1949 |
| BseCI | ATCGAT | 1 cut(s) | 1614 |
| BseGI | GGATG | 6 cut(s) | 321, 828, 1566, 1705, 1795, 1808 |
| BseLI | CCNNNNNNNGG | 9 cut(s) | 528, 582, 694, 819, 823, 1254, 1348, 1452, 1864 |
| BseMI | GCAATG | 1 cut(s) | 2307 |
| BseMII | CTCAG | 5 cut(s) | 42, 382, 1014, 1926, 2156 |
| BseNI | ACTGG | 7 cut(s) | 260, 478, 519, 1053, 1116, 1422, 1481 |
| BseRI | GAGGAG | 3 cut(s) | 568, 610, 710 |
| BseSI | GKGCMC | 4 cut(s) | 576, 689, 1052, 1511 |
| BseX3I | CGGCCG | 2 cut(s) | 291, 489 |
| BseXI | GCAGC | 6 cut(s) | 49, 155, 371, 1351, 1677, 1956 |
| BseYI | CCCAGC | 2 cut(s) | 682, 861 |
| BsgI | GTGCAG | 1 cut(s) | 1851 |
| Bsh1285I | CGRYCG | 5 cut(s) | 294, 492, 1321, 1614, 1736 |
| BshNI | GGYRCC | 2 cut(s) | 647, 2227 |
| BshVI | ATCGAT | 1 cut(s) | 1614 |
| BsiEI | CGRYCG | 5 cut(s) | 294, 492, 1321, 1614, 1736 |
| BsiHKAI | GWGCWC | 2 cut(s) | 420, 1034 |
| BsiHKCI | CYCGRG | 3 cut(s) | 437, 1503, 1864 |
| BslFI | GGGAC | 8 cut(s) | 761, 1269, 1288, 1468, 1608, 1663, 1881, 2012 |
| BslI | CCNNNNNNNGG | 9 cut(s) | 528, 582, 694, 819, 823, 1254, 1348, 1452, 1864 |
| BsmAI | GTCTC | 4 cut(s) | 10, 286, 461, 1615 |
| BsmBI | CGTCTC | 2 cut(s) | 286, 1615 |
| BsmFI | GGGAC | 8 cut(s) | 761, 1269, 1288, 1468, 1608, 1663, 1881, 2012 |
| BsmI | GAATGC | 1 cut(s) | 2242 |
| Bso31I | GGTCTC | 1 cut(s) | 10 |
| BsoBI | CYCGRG | 3 cut(s) | 437, 1503, 1864 |
| Bsp120I | GGGCCC | 3 cut(s) | 685, 1048, 1507 |
| Bsp1286I | GDGCHC | 8 cut(s) | 420, 576, 689, 1034, 1052, 1511, 1721, 2157 |
| Bsp13I | TCCGGA | 1 cut(s) | 1708 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 66 |
| Bsp19I | CCATGG | 2 cut(s) | 900, 1082 |
| BspCNI | CTCAG | 5 cut(s) | 43, 381, 1013, 1927, 2157 |
| BspDI | ATCGAT | 1 cut(s) | 1614 |
| BspEI | TCCGGA | 1 cut(s) | 1708 |
| BspHI | TCATGA | 1 cut(s) | 1743 |
| BspMAI | CTGCAG | 1 cut(s) | 145 |
| BspPI | GGATC | 4 cut(s) | 1094, 1122, 1838, 1851 |
| BspT107I | GGYRCC | 2 cut(s) | 647, 2227 |
| BspTNI | GGTCTC | 1 cut(s) | 10 |
| BsrDI | GCAATG | 1 cut(s) | 2307 |
| BsrFI | RCCGGY | 5 cut(s) | 491, 521, 1229, 1441, 2092 |
| BsrI | ACTGG | 7 cut(s) | 260, 478, 519, 1053, 1116, 1422, 1481 |
| BssAI | RCCGGY | 5 cut(s) | 491, 521, 1229, 1441, 2092 |
| BssNAI | GTATAC | 1 cut(s) | 1758 |
| BssSI | CACGAG | 2 cut(s) | 1027, 2068 |
| BssT1I | CCWWGG | 2 cut(s) | 900, 1082 |
| Bst1107I | GTATAC | 1 cut(s) | 1758 |
| Bst2BI | CACGAG | 2 cut(s) | 1027, 2068 |
| Bst2UI | CCWGG | 6 cut(s) | 340, 583, 652, 807, 1557, 1949 |
| Bst4CI | ACNGT | 9 cut(s) | 46, 1096, 1829, 1888, 2077, 2116, 2149, 2203, 2246 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1783 |
| BstBAI | YACGTR | 3 cut(s) | 594, 1672, 1840 |
| BstDEI | CTNAG | 6 cut(s) | 51, 66, 368, 1000, 1935, 2165 |
| BstF5I | GGATG | 6 cut(s) | 321, 828, 1566, 1705, 1795, 1808 |
| BstH2I | RGCGCY | 5 cut(s) | 651, 1209, 1691, 1742, 2231 |
| BstHHI | GCGC | 9 cut(s) | 650, 723, 785, 805, 1208, 1690, 1705, 1741, 2230 |
| BstMAI | GTCTC | 4 cut(s) | 10, 286, 461, 1615 |
| BstMCI | CGRYCG | 5 cut(s) | 294, 492, 1321, 1614, 1736 |
| BstNI | CCWGG | 6 cut(s) | 340, 583, 652, 807, 1557, 1949 |
| BstNSI | RCATGY | 1 cut(s) | 2282 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 1460 |
| BstSFI | CTRYAG | 1 cut(s) | 141 |
| BstSLI | GKGCMC | 4 cut(s) | 576, 689, 1052, 1511 |
| BstV1I | GCAGC | 6 cut(s) | 49, 155, 371, 1351, 1677, 1956 |
| BstX2I | RGATCY | 1 cut(s) | 1843 |
| BstYI | RGATCY | 1 cut(s) | 1843 |
| BstZ17I | GTATAC | 1 cut(s) | 1758 |
| BstZI | CGGCCG | 2 cut(s) | 291, 489 |
| Bsu15I | ATCGAT | 1 cut(s) | 1614 |
| BsuTUI | ATCGAT | 1 cut(s) | 1614 |
| BtgZI | GCGATG | 2 cut(s) | 1001, 1019 |
| BtrI | CACGTC | 1 cut(s) | 1449 |
| BtsCI | GGATG | 6 cut(s) | 321, 828, 1566, 1705, 1795, 1808 |
| BtsI | GCAGTG | 1 cut(s) | 138 |
| BtsIMutI | CAGTG | 6 cut(s) | 51, 138, 253, 1060, 1109, 2121 |
| CaiI | CAGNNNCTG | 1 cut(s) | 50 |
| CciI | TCATGA | 1 cut(s) | 1743 |
| CfoI | GCGC | 9 cut(s) | 650, 723, 785, 805, 1208, 1690, 1705, 1741, 2230 |
| Cfr10I | RCCGGY | 5 cut(s) | 491, 521, 1229, 1441, 2092 |
| Cfr42I | CCGCGG | 2 cut(s) | 1183, 1324 |
| Cfr9I | CCCGGG | 1 cut(s) | 1864 |
| ClaI | ATCGAT | 1 cut(s) | 1614 |
| CseI | GACGC | 7 cut(s) | 351, 355, 514, 1222, 1436, 1632, 1920 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1588, 1623 |
| CviAII | CATG | 7 cut(s) | 211, 901, 1083, 1267, 1381, 1744, 2279 |
| DdeI | CTNAG | 6 cut(s) | 51, 66, 368, 1000, 1935, 2165 |
| DinI | GGCGCC | 2 cut(s) | 649, 2229 |
| DrdI | GACNNNNNNGTC | 4 cut(s) | 524, 923, 1602, 1657 |
| DriI | GACNNNNNGTC | 2 cut(s) | 1281, 2131 |
| DseDI | GACNNNNNNGTC | 4 cut(s) | 524, 923, 1602, 1657 |
| EaeI | YGGCCR | 9 cut(s) | 81, 213, 291, 454, 489, 530, 733, 1422, 1443 |
| EagI | CGGCCG | 2 cut(s) | 291, 489 |
| Eam1105I | GACNNNNNGTC | 2 cut(s) | 1281, 2131 |
| EciI | GGCGGA | 1 cut(s) | 170 |
| Ecl136II | GAGCTC | 1 cut(s) | 418 |
| EclXI | CGGCCG | 2 cut(s) | 291, 489 |
| Eco130I | CCWWGG | 2 cut(s) | 900, 1082 |
| Eco24I | GRGCYC | 6 cut(s) | 420, 689, 1052, 1511, 1721, 2157 |
| Eco31I | GGTCTC | 1 cut(s) | 10 |
| Eco47I | GGWCC | 4 cut(s) | 432, 566, 1595, 1999 |
| Eco52I | CGGCCG | 2 cut(s) | 291, 489 |
| Eco53kI | GAGCTC | 1 cut(s) | 418 |
| Eco57I | CTGAAG | 1 cut(s) | 1715 |
| Eco72I | CACGTG | 3 cut(s) | 594, 1672, 1840 |
| Eco88I | CYCGRG | 3 cut(s) | 437, 1503, 1864 |
| EcoICRI | GAGCTC | 1 cut(s) | 418 |
| EcoO109I | RGGNCCY | 5 cut(s) | 686, 1049, 1507, 1595, 1999 |
| EcoRI | GAATTC | 1 cut(s) | 958 |
| EcoRII | CCWGG | 6 cut(s) | 338, 581, 650, 805, 1555, 1947 |
| EcoT14I | CCWWGG | 2 cut(s) | 900, 1082 |
| EcoT22I | ATGCAT | 1 cut(s) | 1268 |
| EcoT38I | GRGCYC | 6 cut(s) | 420, 689, 1052, 1511, 1721, 2157 |
| EgeI | GGCGCC | 2 cut(s) | 649, 2229 |
| EheI | GGCGCC | 2 cut(s) | 649, 2229 |
| ErhI | CCWWGG | 2 cut(s) | 900, 1082 |
| Esp3I | CGTCTC | 2 cut(s) | 286, 1615 |
| FaeI | CATG | 7 cut(s) | 214, 904, 1086, 1270, 1384, 1747, 2282 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1004, 1036 |
| FaqI | GGGAC | 8 cut(s) | 761, 1269, 1288, 1468, 1608, 1663, 1881, 2012 |
| FatI | CATG | 7 cut(s) | 210, 900, 1082, 1266, 1380, 1743, 2278 |
| FblI | GTMKAC | 3 cut(s) | 92, 1757, 2048 |
| FokI | GGATG | 6 cut(s) | 308, 835, 1553, 1692, 1795, 1802 |
| FriOI | GRGCYC | 6 cut(s) | 420, 689, 1052, 1511, 1721, 2157 |
| FspBI | CTAG | 2 cut(s) | 1335, 1571 |
| GlaI | GCGC | 9 cut(s) | 649, 722, 784, 804, 1207, 1689, 1704, 1740, 2229 |
| GsaI | CCCAGC | 2 cut(s) | 686, 865 |
| GsuI | CTGGAG | 3 cut(s) | 1464, 1539, 2279 |
| HaeII | RGCGCY | 5 cut(s) | 651, 1209, 1691, 1742, 2231 |
| HgaI | GACGC | 7 cut(s) | 351, 355, 514, 1222, 1436, 1632, 1920 |
| HhaI | GCGC | 9 cut(s) | 650, 723, 785, 805, 1208, 1690, 1705, 1741, 2230 |
| Hin1II | CATG | 7 cut(s) | 214, 904, 1086, 1270, 1384, 1747, 2282 |
| Hin6I | GCGC | 9 cut(s) | 648, 721, 783, 803, 1206, 1688, 1703, 1739, 2228 |
| HinP1I | GCGC | 9 cut(s) | 648, 721, 783, 803, 1206, 1688, 1703, 1739, 2228 |
| HincII | GTYRAC | 3 cut(s) | 1330, 1564, 2049 |
| HindII | GTYRAC | 3 cut(s) | 1330, 1564, 2049 |
| HpaI | GTTAAC | 1 cut(s) | 1330 |
| HphI | GGTGA | 5 cut(s) | 506, 2035, 2089, 2149, 2191 |
| Hpy188III | TCNNGA | 7 cut(s) | 603, 937, 1118, 1286, 1709, 1744, 2251 |
| HpyAV | CCTTC | 6 cut(s) | 26, 125, 201, 1006, 1291, 1690 |
| HpyCH4III | ACNGT | 9 cut(s) | 46, 1096, 1829, 1888, 2077, 2116, 2149, 2203, 2246 |
| HpyCH4V | TGCA | 4 cut(s) | 143, 1266, 1786, 1832 |
| HpyF3I | CTNAG | 6 cut(s) | 51, 66, 368, 1000, 1935, 2165 |
| Hsp92II | CATG | 7 cut(s) | 214, 904, 1086, 1270, 1384, 1747, 2282 |
| HspAI | GCGC | 9 cut(s) | 648, 721, 783, 803, 1206, 1688, 1703, 1739, 2228 |
| KasI | GGCGCC | 2 cut(s) | 647, 2227 |
| KflI | GGGWCCC | 1 cut(s) | 1595 |
| Kpn2I | TCCGGA | 1 cut(s) | 1708 |
| KroI | GCCGGC | 1 cut(s) | 2092 |
| KroNI | GCCGGC | 1 cut(s) | 2094 |
| KspAI | GTTAAC | 1 cut(s) | 1330 |
| KspI | CCGCGG | 2 cut(s) | 1183, 1324 |
| Lsp1109I | GCAGC | 6 cut(s) | 49, 155, 371, 1351, 1677, 1956 |
| LweI | GCATC | 3 cut(s) | 253, 1606, 1714 |
| MaeI | CTAG | 2 cut(s) | 1335, 1571 |
| MboII | GAAGA | 5 cut(s) | 47, 101, 1424, 2152, 2200 |
| MfeI | CAATTG | 1 cut(s) | 625 |
| MflI | RGATCY | 1 cut(s) | 1843 |
| MhlI | GDGCHC | 8 cut(s) | 420, 576, 689, 1034, 1052, 1511, 1721, 2157 |
| MlsI | TGGCCA | 2 cut(s) | 83, 215 |
| MluCI | AATT | 5 cut(s) | 625, 958, 1513, 1921, 2311 |
| MluI | ACGCGT | 1 cut(s) | 1624 |
| MluNI | TGGCCA | 2 cut(s) | 83, 215 |
| Mly113I | GGCGCC | 2 cut(s) | 648, 2228 |
| MlyI | GAGTC | 9 cut(s) | 281, 421, 444, 479, 707, 796, 1291, 1347, 1476 |
| MmeI | TCCRAC | 6 cut(s) | 309, 909, 1215, 1444, 2103, 2247 |
| Mox20I | TGGCCA | 2 cut(s) | 83, 215 |
| Mph1103I | ATGCAT | 1 cut(s) | 1268 |
| MroI | TCCGGA | 1 cut(s) | 1708 |
| MroNI | GCCGGC | 1 cut(s) | 2092 |
| MroXI | GAANNNNTTC | 1 cut(s) | 2246 |
| MscI | TGGCCA | 2 cut(s) | 83, 215 |
| MseI | TTAA | 3 cut(s) | 1022, 1329, 2004 |
| MslI | CAYNNNNRTG | 2 cut(s) | 1094, 1674 |
| Msp20I | TGGCCA | 2 cut(s) | 83, 215 |
| MspA1I | CMGCKG | 6 cut(s) | 62, 146, 764, 1182, 1323, 1526 |
| MunI | CAATTG | 1 cut(s) | 625 |
| Mva1269I | GAATGC | 1 cut(s) | 2242 |
| MvaI | CCWGG | 6 cut(s) | 340, 583, 652, 807, 1557, 1949 |
| NaeI | GCCGGC | 1 cut(s) | 2094 |
| NarI | GGCGCC | 2 cut(s) | 648, 2228 |
| NciI | CCSGG | 8 cut(s) | 381, 780, 1255, 1648, 1721, 1801, 1865, 1866 |
| NcoI | CCATGG | 2 cut(s) | 900, 1082 |
| NgoMIV | GCCGGC | 1 cut(s) | 2092 |
| NlaIII | CATG | 7 cut(s) | 214, 904, 1086, 1270, 1384, 1747, 2282 |
| NmeAIII | GCCGAG | 5 cut(s) | 269, 432, 467, 1055, 1907 |
| NmuCI | GTSAC | 6 cut(s) | 46, 494, 974, 2077, 2116, 2203 |
| NsiI | ATGCAT | 1 cut(s) | 1268 |
| NspI | RCATGY | 1 cut(s) | 2282 |
| OliI | CACNNNNGTG | 2 cut(s) | 1094, 1674 |
| PagI | TCATGA | 1 cut(s) | 1743 |
| PcsI | WCGNNNNNNNCGW | 5 cut(s) | 921, 1607, 2048, 2057, 2129 |
| PctI | GAATGC | 1 cut(s) | 2242 |
| PdiI | GCCGGC | 1 cut(s) | 2094 |
| PdmI | GAANNNNTTC | 1 cut(s) | 2246 |
| PfeI | GAWTC | 9 cut(s) | 132, 263, 561, 599, 707, 840, 1017, 1258, 1729 |
| PflFI | GACNNNGTC | 1 cut(s) | 923 |
| PfoI | TCCNGGA | 3 cut(s) | 581, 1253, 1646 |
| Ple19I | CGATCG | 2 cut(s) | 1614, 1736 |
| PleI | GAGTC | 9 cut(s) | 281, 421, 444, 479, 706, 795, 1290, 1346, 1475 |
| PluTI | GGCGCC | 2 cut(s) | 651, 2231 |
| PmaCI | CACGTG | 3 cut(s) | 594, 1672, 1840 |
| PmlI | CACGTG | 3 cut(s) | 594, 1672, 1840 |
| PpsI | GAGTC | 9 cut(s) | 281, 421, 444, 479, 706, 795, 1290, 1346, 1475 |
| Ppu21I | YACGTR | 3 cut(s) | 594, 1672, 1840 |
| PpuMI | RGGWCCY | 2 cut(s) | 1595, 1999 |
| PshAI | GACNNNNGTC | 1 cut(s) | 1460 |
| Psp124BI | GAGCTC | 1 cut(s) | 420 |
| Psp5II | RGGWCCY | 2 cut(s) | 1595, 1999 |
| Psp6I | CCWGG | 6 cut(s) | 338, 581, 650, 805, 1555, 1947 |
| PspCI | CACGTG | 3 cut(s) | 594, 1672, 1840 |
| PspFI | CCCAGC | 2 cut(s) | 682, 861 |
| PspGI | CCWGG | 6 cut(s) | 338, 581, 650, 805, 1555, 1947 |
| PspOMI | GGGCCC | 3 cut(s) | 685, 1048, 1507 |
| PspPPI | RGGWCCY | 2 cut(s) | 1595, 1999 |
| PstI | CTGCAG | 1 cut(s) | 145 |
| PstNI | CAGNNNCTG | 1 cut(s) | 50 |
| PsuI | RGATCY | 1 cut(s) | 1843 |
| PsyI | GACNNNGTC | 1 cut(s) | 923 |
| PvuI | CGATCG | 2 cut(s) | 1614, 1736 |
| PvuII | CAGCTG | 1 cut(s) | 62 |
| RseI | CAYNNNNRTG | 2 cut(s) | 1094, 1674 |
| SacI | GAGCTC | 1 cut(s) | 420 |
| SacII | CCGCGG | 2 cut(s) | 1183, 1324 |
| SalI | GTCGAC | 1 cut(s) | 2047 |
| SaqAI | TTAA | 3 cut(s) | 1022, 1329, 2004 |
| ScaI | AGTACT | 2 cut(s) | 387, 513 |
| SchI | GAGTC | 9 cut(s) | 281, 421, 444, 479, 707, 796, 1291, 1347, 1476 |
| SduI | GDGCHC | 8 cut(s) | 420, 576, 689, 1034, 1052, 1511, 1721, 2157 |
| SfaNI | GCATC | 3 cut(s) | 253, 1606, 1714 |
| SfcI | CTRYAG | 1 cut(s) | 141 |
| SfoI | GGCGCC | 2 cut(s) | 649, 2229 |
| Sfr303I | CCGCGG | 2 cut(s) | 1183, 1324 |
| SgrAI | CRCCGGYG | 1 cut(s) | 521 |
| SgrBI | CCGCGG | 2 cut(s) | 1183, 1324 |
| SgrDI | CGTCGACG | 1 cut(s) | 2047 |
| SinI | GGWCC | 4 cut(s) | 432, 566, 1595, 1999 |
| SmaI | CCCGGG | 1 cut(s) | 1866 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 1094, 1674 |
| SmlI | CTYRAG | 1 cut(s) | 407 |
| SmoI | CTYRAG | 1 cut(s) | 407 |
| Sse9I | AATT | 5 cut(s) | 625, 958, 1513, 1921, 2311 |
| SspDI | GGCGCC | 2 cut(s) | 647, 2227 |
| SspI | AATATT | 1 cut(s) | 1639 |
| SspMI | CTAG | 2 cut(s) | 1335, 1571 |
| SstI | GAGCTC | 1 cut(s) | 420 |
| StyI | CCWWGG | 2 cut(s) | 900, 1082 |
| TaaI | ACNGT | 9 cut(s) | 46, 1096, 1829, 1888, 2077, 2116, 2149, 2203, 2246 |
| TasI | AATT | 5 cut(s) | 625, 958, 1513, 1921, 2311 |
| TatI | WGTACW | 3 cut(s) | 385, 511, 2064 |
| TfiI | GAWTC | 9 cut(s) | 132, 263, 561, 599, 707, 840, 1017, 1258, 1729 |
| Tru1I | TTAA | 3 cut(s) | 1022, 1329, 2004 |
| Tru9I | TTAA | 3 cut(s) | 1022, 1329, 2004 |
| TscAI | CASTG | 6 cut(s) | 51, 145, 260, 1060, 1116, 2121 |
| TseFI | GTSAC | 6 cut(s) | 46, 494, 974, 2077, 2116, 2203 |
| TseI | GCWGC | 6 cut(s) | 62, 143, 359, 1364, 1690, 1944 |
| Tsp45I | GTSAC | 6 cut(s) | 46, 494, 974, 2077, 2116, 2203 |
| TspGWI | ACGGA | 3 cut(s) | 268, 382, 962 |
| TspMI | CCCGGG | 1 cut(s) | 1864 |
| TspRI | CASTG | 6 cut(s) | 51, 145, 260, 1060, 1116, 2121 |
| Tth111I | GACNNNGTC | 1 cut(s) | 923 |
| VpaK11BI | GGWCC | 4 cut(s) | 432, 566, 1595, 1999 |
| XapI | RAATTY | 2 cut(s) | 958, 1513 |
| XceI | RCATGY | 1 cut(s) | 2282 |
| XmaI | CCCGGG | 1 cut(s) | 1864 |
| XmiI | GTMKAC | 3 cut(s) | 92, 1757, 2048 |
| XmnI | GAANNNNTTC | 1 cut(s) | 2246 |
| XspI | CTAG | 2 cut(s) | 1335, 1571 |
| ZraI | GACGTC | 4 cut(s) | 499, 1653, 2046, 2121 |
| ZrmI | AGTACT | 2 cut(s) | 387, 513 |
| Zsp2I | ATGCAT | 1 cut(s) | 1268 |