pycom16g10030

Pre-mRNA-splicing factor ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0002001
Physical Location & Seq
Forward (+)
6816177 .. 6823428
7252 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3894 bp
ATGGAGAAACGAGGTCACACCGAACCTATTGACCTGGATAAGACAACAGCGACGTTGGAACCTGAGGCGAATAGCGATGGGGGATTATTTGTACCTGGGAAGGATAGAGTTGTTTTTAGACCTCCAGAGAGGAAATCTCTCCTGGGGTTAGATGTCCTTGCTATTGCCAAACGGGAAGGGTCTAAGGTCAACGGTGGATTCAAGACCCCAAGAGAAAGAGGTGCTAATGTTGTCTCATCAATGGAGGAAGACGAAAAATCAGAGTCTGTAATTTCGGATGAAAAAGGTACCGATGAACCCACTGCTATACGAAGTCACTCTAGGAGGCGATATCGGGAAGTATCTGGGAGTGAGACACCACGCACAGAAAGTACTGTAACAGAAGAAGGAAAAATTGATGATATGCATGAGAATCGCTACTCTAGGGAACATCTGCGTGGAGATGTTTCGTCTCCTGGTGGAAGTTCCCACAGCGTCAGGTCTCGGACCCCTAAGTATGACAGGGATGATCGTGGAAATGAGAGAAGAGATTACAAGAGTAGATACGACAGGGATAACCGTGGTAGTGAGAGAAGAGAGTATCAGGATGGTAGTAGGAGTGAAAGACAAAGATATAGTAGTAATGGTAGCGATTATTATCGTGGAAGGGAAGGTAGCCGATATGAACAAGAATATAGTGGAGAATATGGAAGAAAGCGGAGTAGATATGAAGATTCAAAGAGGACACCTGGCAGATCTGACTGGGATGATGGGAGATGGGAATGGGAGGAGTCACCACGACGTGATAACTACTCTAATACCAGTAGGCGACATCAACCATCACCGGCACCTATGCTGCTTGGTGCCTCACCTGATGCACGACTAGTTTCCCCATGGTTAGGTGGCCATACACCTGGTTCTGCTGCTTCTCCTTGGGATCATGTCTCTCCCTCTCCAGCTCCAATACGTGCTTCTGGATACTCAGTCAAATCTTCAAGTTCGAAATATGGTGTGAGGTCCCATCAACTTACTTTTTCTTCAGAAAGTTCACAATCATTCAAGGATGGGGAAGAAGATAATTTGGCCGAAGAACACACATACGAGATTACTGAAAGCATGCGTCAAGAGATGGAATACAATTCTGATCGTGCATGGTATGATAGAGAAGAAGGAAACACAATGTTTGATACGACTGATGACTCGTCCTTGTTTTATGCAAACGATGCTTCATTTCAAAAGAAGGAAGCAGAGTTGGCGAAAAGGCTGGTCCGAAAAGATGGAACTAAGATGTCTCTTGCTCAGAGCAAAAAGTTGTCTCAACTCACTGCTGATAATGCTCAGTGGGAGGATCGTCAACTTTTAAGATCTGGTGCTGTTAGAGGTACCGAGGTGCAGACTGAGTTTGATGATGAAGAAGAACGGAGAGTCATTCTTCTTGTCCATGATACAAAGCCTCCTTTCCTGGACGGCAGGGTTGTTTTTACCAAACAAGCAGAGCCCATAATGCCTATAAAGGATCCCACATCGGATATGGCCATAATTTCACGCAAAGGGTCTACTCTGGTTAGGGAAATTCATGAGAAGCAAAGTCAGAACAAATCACGTCAACGTTTCTGGGAGCTTGCAGGTTCCAAACTAGGTGATATCCTTGGTGTTGAAAAAACGGCGGAGCAGATTGATGCAGATACTGCTGCAGTAGGTGAAGATGGTGAAATTGATTTCAAAGAAGATGCGAAGTTCGCACAACATATGAAGAGCGGGGAGGCTGTGAGTGATTTTGCGTTGTCAAAAACACTCTCACAGCAACGACAATATTTGCCTATATTTTCTGTGCGGGATGAGTTATTGCAGGTAATTCGAGAAAATCAGGTTATTGTTGTTGTTGGAGAAACTGGTTCTGGAAAGACAACTCAATTGACACAGTATCTTTATGAAGATGGATACACAGTAAATGGTATAGTGGGTTGCACCCAACCAAGGCGTGTAGCAGCCATGAGTGTTGCAAAAAGAGTAAGTGAAGAGATGGATACTGAGCTTGGTGATAAAGTTGGCTATGCTATTCGTTTTGAGGATGTGACTGGGCCAAATACCATAATAAAGTACATGACTGATGGAGTACTTCTGCGCGAAACGCTCAGAGATTCTGATCTTGACAAATACCGTGTAGTTGTCATGGACGAAGCCCATGAAAGGTCACTAAACACAGATGTGCTGTTTGGAATTTTGAAAAAAGTGGTGGCCCAACGTCGTGATTTTAAGCTCATTGTGACATCTGCAACTCTAAATGCTCAGAAATTTTCAAATTTCTTTGGGAGTGTACCGATTTTTCACATTCCTGGGAGAACGTTTCCTGTAAATATCTTGTTCAGCAAAACCCCATGTGAAGATTATGTTGAAGGTGCAGTGAAGCAGGCCATGACAATTCACATCACGAGCCCTCCAGGTGATATCCTCATCTTCATGACAGGCCAAGATGAGATTGAGGCAGCTTGTTATGCGCTTGCCGAGCGGATGGAACAACTTATCTCGTCCTCCAAAAAAGCAGTTCCAAAACTCTTGATACTCCCTATATATTCACAGTTGCCTGCTGATTTGCAAGCCAAGATATTCCAAAAAGCTGAGGATGGGGCACGTAAATGCATTGTAGCCACCAACATTGCTGAGACTTCTTTGACTGTGGATGGAATATATTACGTGATCGATACAGGCTATGGTAAGATGAAGGTATACAATCCTCGAATGGGTATGGATGCTCTTCAAGTGTTTCCTGTTAGTCGTGCAGCTGCTGACCAGCGTGCTGGGCGTGCTGGTAGAACTGGGCCTGGGACATGTTATCGGCTGTACACGGAGAATGCATATCTAAATGAATTACTGCCTAGTCCTGTGCCAGAGATTCAGAGGACTAACCTTGGTAATGTGGTGTTGTTGCTCAAGAGTCTTAAGGTCGAGAACTTGTTAGACTTTGATTTCATGGACCCACCTCCCCAGGACAATATTCTCAACTCTATGTACCAATTGTGGGTCTTAGGTGCCCTGAACAATGTCGGAGGCTTAACTGAACTAGGCTGGAAAATGGTGGAGTTCCCATTGGACCCCCCGCTTGCTAAGATGTTATTGATGGGTGACCAGCTTGGATGCTTGGATGAGGTTCTGACAATTGTGTCTATGCTTTCGGTGCCATCAGTGTTTTTTAGGCCTAAAGATAGGGCAGAGGAGAGCGATGCTGCAAGGGAGAAGTTCTCTGTTCCAGAATCTGACCACTTGACGCTGTATAATGTCTACCAGCAGTGGAAACAACATCAGTATCGGGGGGACTGGTGTGGAGATCATTTTTTGCATGTTAAAGGCTTACGAAAGGCTAGAGAGGTGAGGTCCCAACTGCTGGAGATCCTGAAGACGCTAAAAATTCCTCTAACATCCTGTTGGCCCGATACTGATCTTGTGAGAAAAGCCATATGCTCCTCGTACTTCCACAATTCTGCAAGATTGAAGGGAGTTGGGGAGTATGTGAACTGCAGGACCGGGATGCCATGCCATTTACATCCGAGCAGTGCTCTTTACGGTATGGGGTGCACTCCAGATTATGTGGTTTATCATGAACTGATTTTGACGGCAAAGGAGTACATGCAGTGTGCCACAGCGGTGGAGCCGCAGTGGTTGGCCGAGTTGGGGCCCATGTTCTTTTCTGTGAAGGATTCAGATACATCGATGCTGGAGCATAAGAAGAGACAAAAGGAGGAGAAAACAGCGATGGAGGAGGAAATGGAGAACCTGAGGAAGACTCAAGCGGAGGAAGAGAAAGAAAGCAAGCGGAAGGAGAGGGAAAAGAGATCAAAGCAGCAGCAGCAAGTCTCGATGCCAGGTTTGCACAAGGGATCTTCTACATATTTGAGGCCAAAGAAACTTGGCTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000375 GO:0000377 GO:0000398 GO:0002376 GO:0003002 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005681 GO:0005737 GO:0006139 GO:0006396 GO:0006397 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006950 GO:0006952 GO:0006955 GO:0007275 GO:0007389 GO:0008026 GO:0008104 GO:0008150 GO:0008152 GO:0008186 GO:0008380 GO:0009605 GO:0009607 GO:0009620 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009814 GO:0009817 GO:0009888 GO:0009892 GO:0009893 GO:0009987 GO:0010015 GO:0010033 GO:0010051 GO:0010053 GO:0010054 GO:0010154 GO:0010467 GO:0010468 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010629 GO:0014070 GO:0015031 GO:0015833 GO:0015931 GO:0016049 GO:0016070 GO:0016071 GO:0016246 GO:0016441 GO:0016458 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0021700 GO:0022414 GO:0022622 GO:0030154 GO:0031047 GO:0031050 GO:0031053 GO:0031123 GO:0031124 GO:0031323 GO:0031325 GO:0031503 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0033036 GO:0033120 GO:0034470 GO:0034613 GO:0034641 GO:0034660 GO:0035194 GO:0035195 GO:0035196 GO:0040007 GO:0040029 GO:0042221 GO:0042623 GO:0042886 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043331 GO:0043484 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045087 GO:0045184 GO:0045935 GO:0046483 GO:0046907 GO:0048316 GO:0048364 GO:0048468 GO:0048469 GO:0048518 GO:0048519 GO:0048522 GO:0048588 GO:0048589 GO:0048608 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048856 GO:0048869 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051028 GO:0051168 GO:0051169 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051236 GO:0051252 GO:0051254 GO:0051641 GO:0051649 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0060560 GO:0061458 GO:0065007 GO:0070013 GO:0070035 GO:0070727 GO:0070887 GO:0070918 GO:0071013 GO:0071166 GO:0071310 GO:0071359 GO:0071407 GO:0071426 GO:0071427 GO:0071695 GO:0071702 GO:0071704 GO:0071705 GO:0080090 GO:0080147 GO:0090304 GO:0090558 GO:0090627 GO:0097159 GO:0098542 GO:0099402 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1902494 GO:1905392 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind

Protein Analysis

1298

Amino Acids

146.77

Weight (kDa)

6.03

Isoelectric Point (pI)

51.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 607 - 757 1.3e-08 DEAD/DEAH box helicase
AAA_22 PF13401 614 - 747 9.2e-06 AAA domain
Helicase_C PF00271 804 - 933 4.3e-11 Helicase conserved C-terminal domain
WHD_HA2 PF04408 998 - 1023 5.3e-07 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 1024 - 1083 4.3e-16 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 1141 - 1218 7.6e-18 Oligonucleotide/oligosaccharide-binding (OB)-fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 3139
Acc36I ACCTGC 2 cut(s) 1595, 1819
Acc65I GGTACC 2 cut(s) 287, 1361
AccB1I GGYRCC 6 cut(s) 287, 826, 842, 1361, 3008, 3154
AccB7I CCANNNNNTGG 1 cut(s) 3361
AccBSI CCGCTC 2 cut(s) 1737, 2488
AccI GTMKAC 3 cut(s) 1535, 2706, 3258
AccII CGCG 1 cut(s) 2106
AclI AACGTT 2 cut(s) 1588, 2324
AclWI GGATC 6 cut(s) 924, 1335, 1490, 1503, 3361, 3862
AcoI YGGCCR 4 cut(s) 883, 1062, 1512, 3639
AcsI RAATTY 5 cut(s) 1551, 2199, 2273, 2281, 3384
AcuI CTGAAG 2 cut(s) 1002, 3392
AdeI CACNNNGTG 1 cut(s) 782
AfiI CCNNNNNNNGG 7 cut(s) 878, 1956, 2169, 2720, 2998, 3361, 3648
AflII CTTAAG 1 cut(s) 2918
AflIII ACRYGT 1 cut(s) 2807
AhlI ACTAGT 1 cut(s) 862
AjiI CACGTC 2 cut(s) 782, 1583
AjuI GAANNNNNNNTTGG 2 cut(s) 934, 966
AleI CACNNNNGTG 2 cut(s) 2186, 3620
AluBI AGCT 8 cut(s) 938, 1600, 2014, 2239, 2468, 2597, 2762, 3109
AluI AGCT 8 cut(s) 938, 1600, 2014, 2239, 2468, 2597, 2762, 3109
Alw21I GWGCWC 2 cut(s) 3535, 3554
Alw44I GTGCAC 1 cut(s) 3550
AlwI GGATC 6 cut(s) 924, 1335, 1490, 1503, 3361, 3862
AlwNI CAGNNNCTG 4 cut(s) 266, 1667, 2765, 3233
ApaI GGGCCC 1 cut(s) 3654
ApaLI GTGCAC 1 cut(s) 3550
ApoI RAATTY 5 cut(s) 1551, 2199, 2273, 2281, 3384
Asp718I GGTACC 2 cut(s) 287, 1361
AspLEI GCGC 2 cut(s) 2106, 2479
AsuC2I CCSGG 1 cut(s) 3502
AsuII TTCGAA 1 cut(s) 980
AvaII GGWCC 7 cut(s) 486, 996, 1246, 2953, 3070, 3351, 3498
AxyI CCTNAGG 2 cut(s) 63, 3752
BaeGI GKGCMC 4 cut(s) 2611, 3013, 3554, 3654
BaeI ACNNNNGTAYC 4 cut(s) 1353, 1386, 3266, 3299
BalI TGGCCA 2 cut(s) 885, 1514
BamHI GGATCC 1 cut(s) 1495
BanI GGYRCC 6 cut(s) 287, 826, 842, 1361, 3008, 3154
BanII GRGCYC 3 cut(s) 1479, 2417, 3654
BauI CACGAG 1 cut(s) 2410
BbsI GAAGAC 3 cut(s) 255, 3380, 3764
Bbv12I GWGCWC 2 cut(s) 3535, 3554
BbvCI CCTCAGC 1 cut(s) 2598
BceAI ACGGC 3 cut(s) 1462, 1659, 3606
BcgI CGANNNNNNTGC 4 cut(s) 395, 429, 1817, 1851
BciVI GTATCC 3 cut(s) 950, 1913, 1999
BcnI CCSGG 1 cut(s) 3502
BcuI ACTAGT 1 cut(s) 862
BfaI CTAG 7 cut(s) 321, 423, 863, 1616, 2856, 3041, 3339
BfmI CTRYAG 2 cut(s) 1671, 3493
BfrI CTTAAG 1 cut(s) 2918
BfuAI ACCTGC 2 cut(s) 1595, 1819
BfuI GTATCC 3 cut(s) 950, 1913, 1999
BglII AGATCT 2 cut(s) 734, 1343
BmcAI AGTACT 2 cut(s) 373, 2097
Bme18I GGWCC 7 cut(s) 486, 996, 1246, 2953, 3070, 3351, 3498
BmgBI CACGTC 2 cut(s) 782, 1583
BmrI ACTGGG 3 cut(s) 751, 2067, 2805
BmuI ACTGGG 3 cut(s) 751, 2067, 2805
BpiI GAAGAC 3 cut(s) 255, 3380, 3764
BplI GAGNNNNNCTC 8 cut(s) 121, 153, 3203, 3235, 3517, 3549, 3745, 3777
BpmI CTGGAG 6 cut(s) 108, 918, 2403, 3383, 3540, 3713
Bpu10I CCTNAGC 1 cut(s) 2598
Bpu14I TTCGAA 1 cut(s) 980
BpuEI CTTGAG 2 cut(s) 2894, 3747
BpuMI CCSGG 1 cut(s) 3502
Bsa29I ATCGAT 2 cut(s) 2679, 3686
BsaAI YACGTR 3 cut(s) 947, 2612, 2674
BsaBI GATNNNNATC 3 cut(s) 636, 2124, 3414
BsaI GGTCTC 1 cut(s) 486
BsaXI ACNNNNNCTCC 6 cut(s) 1417, 1447, 2401, 2431, 3617, 3647
Bsc4I CCNNNNNNNGG 7 cut(s) 878, 1956, 2169, 2720, 2998, 3361, 3648
Bse118I RCCGGY 1 cut(s) 823
Bse1I ACTGG 6 cut(s) 746, 801, 1876, 2062, 2800, 3299
Bse21I CCTNAGG 2 cut(s) 63, 3752
Bse3DI GCAATG 1 cut(s) 2634
Bse8I GATNNNNATC 3 cut(s) 636, 2124, 3414
BseCI ATCGAT 2 cut(s) 2679, 3686
BseJI GATNNNNATC 3 cut(s) 636, 2124, 3414
BseLI CCNNNNNNNGG 7 cut(s) 878, 1956, 2169, 2720, 2998, 3361, 3648
BseMI GCAATG 1 cut(s) 2634
BseNI ACTGG 6 cut(s) 746, 801, 1876, 2062, 2800, 3299
BseRI GAGGAG 5 cut(s) 782, 3206, 3430, 3731, 3749
BseSI GKGCMC 4 cut(s) 2611, 3013, 3554, 3654
BseYI CCCAGC 1 cut(s) 2777
BsgI GTGCAG 3 cut(s) 1391, 2400, 2778
Bsh1236I CGCG 1 cut(s) 2106
BshNI GGYRCC 6 cut(s) 287, 826, 842, 1361, 3008, 3154
BshVI ATCGAT 2 cut(s) 2679, 3686
BsiHKAI GWGCWC 2 cut(s) 3535, 3554
BsiSI CCGG 2 cut(s) 824, 3501
BslFI GGGAC 4 cut(s) 982, 2818, 3305, 3337
BslI CCNNNNNNNGG 7 cut(s) 878, 1956, 2169, 2720, 2998, 3361, 3648
BsmBI CGTCTC 1 cut(s) 456
BsmFI GGGAC 4 cut(s) 982, 2818, 3305, 3337
BsmI GAATGC 1 cut(s) 2836
Bso31I GGTCTC 1 cut(s) 486
Bsp119I TTCGAA 1 cut(s) 980
Bsp120I GGGCCC 1 cut(s) 3650
Bsp1286I GDGCHC 7 cut(s) 1479, 2417, 2611, 3013, 3535, 3554, 3654
Bsp1407I TGTACA 1 cut(s) 2820
Bsp19I CCATGG 1 cut(s) 872
BspDI ATCGAT 2 cut(s) 2679, 3686
BspFNI CGCG 1 cut(s) 2106
BspHI TCATGA 3 cut(s) 1555, 2439, 3574
BspMAI CTGCAG 2 cut(s) 1675, 3497
BspMI ACCTGC 2 cut(s) 1595, 1819
BspPI GGATC 6 cut(s) 924, 1335, 1490, 1503, 3361, 3862
BspQI GCTCTTC 2 cut(s) 1727, 2739
BspT104I TTCGAA 1 cut(s) 980
BspT107I GGYRCC 6 cut(s) 287, 826, 842, 1361, 3008, 3154
BspTI CTTAAG 1 cut(s) 2918
BspTNI GGTCTC 1 cut(s) 486
BsrBI CCGCTC 2 cut(s) 1737, 2488
BsrDI GCAATG 1 cut(s) 2634
BsrFI RCCGGY 1 cut(s) 823
BsrGI TGTACA 1 cut(s) 2820
BsrI ACTGG 6 cut(s) 746, 801, 1876, 2062, 2800, 3299
BssAI RCCGGY 1 cut(s) 823
BssNAI GTATAC 1 cut(s) 2707
BssSI CACGAG 1 cut(s) 2410
BssT1I CCWWGG 5 cut(s) 872, 911, 1627, 1955, 2887
Bst1107I GTATAC 1 cut(s) 2707
Bst2BI CACGAG 1 cut(s) 2410
Bst4CI ACNGT 9 cut(s) 194, 376, 560, 1902, 1927, 2141, 2559, 2656, 3542
Bst6I CTCTTC 7 cut(s) 520, 568, 1727, 1992, 2739, 3698, 3768
BstAFI CTTAAG 1 cut(s) 2918
BstAPI GCANNNNNTGC 2 cut(s) 1202, 1667
BstAUI TGTACA 1 cut(s) 2820
BstBAI YACGTR 3 cut(s) 947, 2612, 2674
BstBI TTCGAA 1 cut(s) 980
BstDSI CCRYGG 2 cut(s) 559, 872
BstEII GGTNACC 1 cut(s) 3101
BstFNI CGCG 1 cut(s) 2106
BstHHI GCGC 2 cut(s) 2106, 2479
BstNSI RCATGY 4 cut(s) 1099, 2811, 3320, 3607
BstPI GGTNACC 1 cut(s) 3101
BstSFI CTRYAG 2 cut(s) 1671, 3493
BstSLI GKGCMC 4 cut(s) 2611, 3013, 3554, 3654
BstUI CGCG 1 cut(s) 2106
BstV2I GAAGAC 3 cut(s) 255, 3380, 3764
BstX2I RGATCY 5 cut(s) 734, 1343, 1495, 3366, 3854
BstXI CCANNNNNNTGG 3 cut(s) 893, 2777, 3622
BstYI RGATCY 5 cut(s) 734, 1343, 1495, 3366, 3854
BstZ17I GTATAC 1 cut(s) 2707
Bsu15I ATCGAT 2 cut(s) 2679, 3686
Bsu36I CCTNAGG 2 cut(s) 63, 3752
BsuI GTATCC 3 cut(s) 950, 1913, 1999
BsuTUI ATCGAT 2 cut(s) 2679, 3686
BtgI CCRYGG 2 cut(s) 559, 872
BtgZI GCGATG 3 cut(s) 90, 3213, 3743
BtrI CACGTC 2 cut(s) 782, 1583
BtsI GCAGTG 7 cut(s) 300, 1302, 2388, 3272, 3535, 3614, 3638
BtsIMutI CAGTG 9 cut(s) 300, 1302, 1325, 2388, 3168, 3272, 3535, 3614, 3638
BveI ACCTGC 2 cut(s) 1595, 1819
CaiI CAGNNNCTG 4 cut(s) 266, 1667, 2765, 3233
CciI TCATGA 3 cut(s) 1555, 2439, 3574
CfoI GCGC 2 cut(s) 2106, 2479
Cfr10I RCCGGY 1 cut(s) 823
ClaI ATCGAT 2 cut(s) 2679, 3686
CseI GACGC 4 cut(s) 463, 1088, 3253, 3385
CsiI ACCWGGT 1 cut(s) 892
CspCI CAANNNNNGTGG 2 cut(s) 2617, 2652
DraIII CACNNNGTG 1 cut(s) 782
DrdI GACNNNNNNGTC 1 cut(s) 3139
DseDI GACNNNNNNGTC 1 cut(s) 3139
EaeI YGGCCR 4 cut(s) 883, 1062, 1512, 3639
Eam1104I CTCTTC 7 cut(s) 520, 568, 1727, 1992, 2739, 3698, 3768
EarI CTCTTC 7 cut(s) 520, 568, 1727, 1992, 2739, 3698, 3768
EciI GGCGGA 1 cut(s) 1661
Eco130I CCWWGG 5 cut(s) 872, 911, 1627, 1955, 2887
Eco147I AGGCCT 1 cut(s) 3175
Eco24I GRGCYC 3 cut(s) 1479, 2417, 3654
Eco31I GGTCTC 1 cut(s) 486
Eco32I GATATC 3 cut(s) 332, 1624, 2428
Eco47I GGWCC 7 cut(s) 486, 996, 1246, 2953, 3070, 3351, 3498
Eco57I CTGAAG 2 cut(s) 1002, 3392
Eco81I CCTNAGG 2 cut(s) 63, 3752
Eco91I GGTNACC 1 cut(s) 3101
EcoO109I RGGNCCY 3 cut(s) 996, 3351, 3650
EcoO65I GGTNACC 1 cut(s) 3101
EcoRV GATATC 3 cut(s) 332, 1624, 2428
EcoT14I CCWWGG 5 cut(s) 872, 911, 1627, 1955, 2887
EcoT22I ATGCAT 3 cut(s) 408, 2621, 2836
EcoT38I GRGCYC 3 cut(s) 1479, 2417, 3654
ErhI CCWWGG 5 cut(s) 872, 911, 1627, 1955, 2887
Esp3I CGTCTC 1 cut(s) 456
FaqI GGGAC 4 cut(s) 982, 2818, 3305, 3337
FauI CCCGC 3 cut(s) 1730, 1806, 3084
FauNDI CATATG 2 cut(s) 1728, 3434
FblI GTMKAC 3 cut(s) 1535, 2706, 3258
FriOI GRGCYC 3 cut(s) 1479, 2417, 3654
FspBI CTAG 7 cut(s) 321, 423, 863, 1616, 2856, 3041, 3339
GlaI GCGC 2 cut(s) 2105, 2478
GsaI CCCAGC 1 cut(s) 2781
GsuI CTGGAG 6 cut(s) 108, 918, 2403, 3383, 3540, 3713
HapII CCGG 2 cut(s) 824, 3501
HgaI GACGC 4 cut(s) 463, 1088, 3253, 3385
HhaI GCGC 2 cut(s) 2106, 2479
Hin6I GCGC 2 cut(s) 2104, 2477
HinP1I GCGC 2 cut(s) 2104, 2477
HincII GTYRAC 3 cut(s) 190, 1334, 1586
HindII GTYRAC 3 cut(s) 190, 1334, 1586
HpaII CCGG 2 cut(s) 824, 3501
Hpy99I CGWCG 3 cut(s) 55, 783, 2229
HpyCH4III ACNGT 9 cut(s) 194, 376, 560, 1902, 1927, 2141, 2559, 2656, 3542
HpyCH4IV ACGT 9 cut(s) 53, 781, 946, 1582, 1588, 2224, 2324, 2611, 2673
HpySE526I ACGT 9 cut(s) 53, 781, 946, 1582, 1588, 2224, 2324, 2611, 2673
HspAI GCGC 2 cut(s) 2104, 2477
KpnI GGTACC 2 cut(s) 291, 1365
LguI GCTCTTC 2 cut(s) 1727, 2739
LmnI GCTCC 6 cut(s) 943, 1597, 1648, 3443, 3625, 3694
MabI ACCWGGT 1 cut(s) 892
MaeI CTAG 7 cut(s) 321, 423, 863, 1616, 2856, 3041, 3339
MaeII ACGT 9 cut(s) 53, 781, 946, 1582, 1588, 2224, 2324, 2611, 2673
MaeIII GTNAC 8 cut(s) 14, 314, 376, 771, 2053, 2172, 2245, 3101
MbiI CCGCTC 2 cut(s) 1737, 2488
MfeI CAATTG 3 cut(s) 1892, 2993, 3135
MflI RGATCY 5 cut(s) 734, 1343, 1495, 3366, 3854
MhlI GDGCHC 7 cut(s) 1479, 2417, 2611, 3013, 3535, 3554, 3654
MlsI TGGCCA 2 cut(s) 885, 1514
MluNI TGGCCA 2 cut(s) 885, 1514
MlyI GAGTC 6 cut(s) 272, 779, 1172, 1413, 2923, 3754
MmeI TCCRAC 3 cut(s) 36, 1843, 3004
Mox20I TGGCCA 2 cut(s) 885, 1514
Mph1103I ATGCAT 3 cut(s) 408, 2621, 2836
MscI TGGCCA 2 cut(s) 885, 1514
MseI TTAA 5 cut(s) 1340, 2235, 2919, 3032, 3321
MslI CAYNNNNRTG 5 cut(s) 435, 2186, 2438, 2614, 3620
Msp20I TGGCCA 2 cut(s) 885, 1514
MspA1I CMGCKG 2 cut(s) 2762, 3620
MspCI CTTAAG 1 cut(s) 2918
MspI CCGG 2 cut(s) 824, 3501
MunI CAATTG 3 cut(s) 1892, 2993, 3135
Mva1269I GAATGC 1 cut(s) 2836
MvnI CGCG 1 cut(s) 2106
NciI CCSGG 1 cut(s) 3502
NcoI CCATGG 1 cut(s) 872
NdeI CATATG 2 cut(s) 1728, 3434
NmeAIII GCCGAG 2 cut(s) 2509, 3667
NmuCI GTSAC 7 cut(s) 14, 314, 771, 2053, 2172, 2245, 3101
NsiI ATGCAT 3 cut(s) 408, 2621, 2836
NspI RCATGY 4 cut(s) 1099, 2811, 3320, 3607
NspV TTCGAA 1 cut(s) 980
OliI CACNNNNGTG 2 cut(s) 2186, 3620
PaeI GCATGC 1 cut(s) 1099
PagI TCATGA 3 cut(s) 1555, 2439, 3574
PceI AGGCCT 1 cut(s) 3175
PciI ACATGT 1 cut(s) 2807
PciSI GCTCTTC 2 cut(s) 1727, 2739
PctI GAATGC 1 cut(s) 2836
PfeI GAWTC 7 cut(s) 198, 412, 713, 2120, 2872, 3230, 3674
PflMI CCANNNNNTGG 1 cut(s) 3361
PfoI TCCNGGA 1 cut(s) 1440
PleI GAGTC 6 cut(s) 271, 778, 1172, 1412, 2922, 3754
PpsI GAGTC 6 cut(s) 271, 778, 1172, 1412, 2922, 3754
Ppu21I YACGTR 3 cut(s) 947, 2612, 2674
PpuMI RGGWCCY 2 cut(s) 996, 3351
PscI ACATGT 1 cut(s) 2807
Psp1406I AACGTT 2 cut(s) 1588, 2324
Psp5II RGGWCCY 2 cut(s) 996, 3351
PspEI GGTNACC 1 cut(s) 3101
PspFI CCCAGC 1 cut(s) 2777
PspOMI GGGCCC 1 cut(s) 3650
PspPPI RGGWCCY 2 cut(s) 996, 3351
PstI CTGCAG 2 cut(s) 1675, 3497
PstNI CAGNNNCTG 4 cut(s) 266, 1667, 2765, 3233
PsuI RGATCY 5 cut(s) 734, 1343, 1495, 3366, 3854
PvuII CAGCTG 1 cut(s) 2762
RseI CAYNNNNRTG 5 cut(s) 435, 2186, 2438, 2614, 3620
SapI GCTCTTC 2 cut(s) 1727, 2739
SaqAI TTAA 5 cut(s) 1340, 2235, 2919, 3032, 3321
ScaI AGTACT 2 cut(s) 373, 2097
SchI GAGTC 6 cut(s) 272, 779, 1172, 1413, 2923, 3754
SduI GDGCHC 7 cut(s) 1479, 2417, 2611, 3013, 3535, 3554, 3654
SexAI ACCWGGT 1 cut(s) 892
SfcI CTRYAG 2 cut(s) 1671, 3493
SfuI TTCGAA 1 cut(s) 980
SinI GGWCC 7 cut(s) 486, 996, 1246, 2953, 3070, 3351, 3498
SmiMI CAYNNNNRTG 5 cut(s) 435, 2186, 2438, 2614, 3620
SmlI CTYRAG 3 cut(s) 2909, 2918, 3762
SmoI CTYRAG 3 cut(s) 2909, 2918, 3762
SpeI ACTAGT 1 cut(s) 862
SphI GCATGC 1 cut(s) 1099
SseBI AGGCCT 1 cut(s) 3175
SspI AATATT 2 cut(s) 1793, 2974
SspMI CTAG 7 cut(s) 321, 423, 863, 1616, 2856, 3041, 3339
StuI AGGCCT 1 cut(s) 3175
StyI CCWWGG 5 cut(s) 872, 911, 1627, 1955, 2887
TaaI ACNGT 9 cut(s) 194, 376, 560, 1902, 1927, 2141, 2559, 2656, 3542
TaiI ACGT 9 cut(s) 56, 784, 949, 1585, 1591, 2227, 2327, 2614, 2676
TaqI TCGA 7 cut(s) 980, 1837, 2679, 2716, 2925, 3686, 3833
TatI WGTACW 5 cut(s) 371, 2079, 2095, 2820, 3600
TauI GCSGC 1 cut(s) 3631
TfiI GAWTC 7 cut(s) 198, 412, 713, 2120, 2872, 3230, 3674
Tru1I TTAA 5 cut(s) 1340, 2235, 2919, 3032, 3321
Tru9I TTAA 5 cut(s) 1340, 2235, 2919, 3032, 3321
TscAI CASTG 9 cut(s) 307, 1309, 1325, 2388, 3168, 3272, 3535, 3614, 3638
TseFI GTSAC 7 cut(s) 14, 314, 771, 2053, 2172, 2245, 3101
Tsp45I GTSAC 7 cut(s) 14, 314, 771, 2053, 2172, 2245, 3101
TspGWI ACGGA 2 cut(s) 1414, 2840
TspRI CASTG 9 cut(s) 307, 1309, 1325, 2388, 3168, 3272, 3535, 3614, 3638
Van91I CCANNNNNTGG 1 cut(s) 3361
Vha464I CTTAAG 1 cut(s) 2918
VneI GTGCAC 1 cut(s) 3550
VpaK11BI GGWCC 7 cut(s) 486, 996, 1246, 2953, 3070, 3351, 3498
XapI RAATTY 5 cut(s) 1551, 2199, 2273, 2281, 3384
XceI RCATGY 4 cut(s) 1099, 2811, 3320, 3607
XcmI CCANNNNNNNNNTGG 1 cut(s) 1507
XmiI GTMKAC 3 cut(s) 1535, 2706, 3258
XspI CTAG 7 cut(s) 321, 423, 863, 1616, 2856, 3041, 3339
ZrmI AGTACT 2 cut(s) 373, 2097
Zsp2I ATGCAT 3 cut(s) 408, 2621, 2836
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.