pycom16g25150

sphingolipid transporter spinster homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0001699
Physical Location & Seq
Forward (+)
26962394 .. 26967808
5415 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1611 bp
ATGTGTCCTGAGAGAGAGGGGAGGGAGGGGGCAGAGGCAGAGGGTGCTGTAAACTTTGGAGGTTGCTTGCGGAACAAGGCTCTCTTTCTCCACCCCTCCCTCTTCTCTCCTCTCTCTCATAATCTCATCATTATTATGGCGGAATCACAACAACGAGAGAAGCAACAGGAGCAGCACCACCATCAAGCGCCTCCTCCAATCCCTACTCCGCCGTCCGATCCTCCCAACCCAGATATGTCGGAGAGCCCCATCTCAGACTCACCTCGATATCCATCATGGTTCACCCCCAAAAGGTTGCTCGTGATGTTCTGTGTGATTAACTTGATTAATTATGTCGATCGAGGAGCAATAGCCAGCAATGGTGTCAACGGTAGCATTGGGGTTTGTGATCCTAGCGGCATTTGTACCGCGGGTACCGGAATTCAGGGAGATTTTAAATTGAGCAATTTTGAAGACGGCTCTCTGTCATCTGCATTTATGGTTGGCCTTCTTGTGGCCTCACCCATCTTTGCATCGTTGGCAAAGAGTCACAATCCTTTTAGGCTGATTGGAGTTGGATTATCTATTTGGACATTTGCCACAGCCGGGTGTGGTGGTTCAATTAACTTTTGGACCATTGCAATATGTCGCATGCTGGTTGGAGTTGGTGAGGCATCTTTCATTAGTCTTGCAGCACCATTCATCGATGACCATGCTCCTGCAACTCAGAAAACAGCATGGCTTGCTATGTTTTACATGTGTATACCAACTGGAATTGCCGTGGGCTATGTTTATGGTGGATTTGTGGGATCCAGTTTCAGTTGGCGTTATGCATTCTGGGGAGAGGCATTAAGTATGCTTCCATTTTGTGTATTGGCTTTTGTGATGAAACCATTGGAGTTGAAAGGTCTTGCTCCTGCTGACACAAGAAAAACTTTGGAACCTATTGAAACAACTAGTCCTTTAATTGAAGATTCAGATGTCACAGAAAAAGCTTCTTGGTCAAGCCCTGCTACAAAGTCTTTGAGCCAACTCTCAAGATTCTCAAGAGATATGAAAGCGCTTTTGCTCGATAAAGTTTATGTTGTTAATGTCTTAGGTTATGTGTCATATAACTTTGTCATTGGAGCCTACTCGTACTGGGGACCAAAGGCTGGTTATGACATTTATCATATGAATAAGCCAGATTTGTGGTTCGGAGGTATTACAATTGTTTGTGGAATTCTGGGGACGTTAGCTGGAGGGCTCATCCTTGATGCAGTGACTGCCACAATTTATAACGCTTTTAAGCTTCTCTCTGTTGCAACATTTCTAGGCGCAATATTTTGCTTCAGTGCCTTCTGTGTAAGGAACTTGTATGGTTTTGTAGTTCTCTTCGCATTGGGGGAGCTCCTTGTGTTTGCAACCCAGGCTCCAGTTAATTATGTGTCTCTGCAGTGCGTGACACCAAGTCTTAGGCCATTGGCTATGGCTGTCTCAACTGTTTCAATCCACATCTTTGGTGATGTTCCTTCCTCGCCTCTTGCTGGAATACTCCAGGACCATGTTAAAAATTGGAGGATAACAACTCTTGCTGTGACATCTGTTCTGTTTCTTGCGTCTGGAATATGGTTCATAGGGATCGTTCGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MFS_1 OATP

Protein Analysis

537

Amino Acids

57.91

Weight (kDa)

6.37

Isoelectric Point (pI)

35.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFS_1 PF07690 140 - 489 1.2e-30 Major Facilitator Superfamily
MFS_4 PF06779 147 - 305 3.3e-06 Uncharacterised MFS-type transporter YbfB
Sugar_tr PF00083 150 - 496 3.9e-06 Sugar (and other) transporter
OATP PF03137 205 - 441 5e-18 Organic Anion Transporter Polypeptide (OATP) family
OATP PF03137 440 - 508 4.8e-07 Organic Anion Transporter Polypeptide (OATP) family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1257
Acc65I GGTACC 1 cut(s) 413
AccB1I GGYRCC 1 cut(s) 413
AccB7I CCANNNNNTGG 1 cut(s) 1133
AccI GTMKAC 1 cut(s) 742
AccII CGCG 1 cut(s) 410
AciI CCGC 6 cut(s) 70, 140, 209, 396, 408, 410
AclWI GGATC 5 cut(s) 212, 383, 783, 796, 1607
AcsI RAATTY 2 cut(s) 420, 1200
AcuI CTGAAG 1 cut(s) 1294
AfaI GTAC 3 cut(s) 406, 415, 1118
AfeI AGCGCT 1 cut(s) 1041
AfiI CCNNNNNNNGG 5 cut(s) 291, 493, 585, 1133, 1505
AflIII ACRYGT 1 cut(s) 735
AgsI TTSAA 6 cut(s) 452, 600, 883, 929, 950, 1467
AhdI GACNNNNNGTC 1 cut(s) 1428
AhlI ACTAGT 1 cut(s) 935
AjnI CCWGG 2 cut(s) 1386, 1515
AluBI AGCT 4 cut(s) 974, 1217, 1270, 1369
AluI AGCT 4 cut(s) 974, 1217, 1270, 1369
Alw21I GWGCWC 1 cut(s) 1371
Alw26I GTCTC 2 cut(s) 1413, 1459
AlwI GGATC 5 cut(s) 212, 383, 783, 796, 1607
AlwNI CAGNNNCTG 1 cut(s) 1244
Aor51HI AGCGCT 1 cut(s) 1041
AoxI GGCC 3 cut(s) 484, 495, 1436
ApeKI GCWGC 2 cut(s) 172, 671
ApoI RAATTY 2 cut(s) 420, 1200
AseI ATTAAT 1 cut(s) 327
Asp718I GGTACC 1 cut(s) 413
AspLEI GCGC 3 cut(s) 190, 1042, 1298
AspS9I GGNCC 3 cut(s) 612, 1124, 1519
AsuC2I CCSGG 1 cut(s) 586
AsuHPI GGTGA 5 cut(s) 252, 274, 492, 659, 1493
AvaII GGWCC 3 cut(s) 612, 1124, 1519
BamHI GGATCC 1 cut(s) 788
BanI GGYRCC 1 cut(s) 413
BanII GRGCYC 3 cut(s) 248, 1227, 1371
BauI CACGAG 1 cut(s) 299
BbsI GAAGAC 1 cut(s) 459
Bbv12I GWGCWC 1 cut(s) 1371
BbvI GCAGC 2 cut(s) 184, 683
BccI CCATC 4 cut(s) 189, 257, 280, 512
BceAI ACGGC 3 cut(s) 196, 472, 743
BcgI CGANNNNNNTGC 2 cut(s) 674, 708
BciT130I CCWGG 2 cut(s) 1388, 1517
BcnI CCSGG 1 cut(s) 586
BcoDI GTCTC 2 cut(s) 1413, 1459
BcuI ACTAGT 1 cut(s) 935
BfaI CTAG 3 cut(s) 393, 936, 1292
BfmI CTRYAG 1 cut(s) 1412
BfoI RGCGCY 2 cut(s) 191, 1043
BisI GCNGC 3 cut(s) 173, 397, 672
BlsI GCNGC 3 cut(s) 174, 398, 673
Bme1390I CCNGG 3 cut(s) 586, 1388, 1517
Bme18I GGWCC 3 cut(s) 612, 1124, 1519
BmeRI GACNNNNNGTC 1 cut(s) 1428
BmgT120I GGNCC 3 cut(s) 612, 1124, 1519
BmiI GGNNCC 6 cut(s) 415, 790, 921, 1108, 1125, 1392
BmrFI CCNGG 3 cut(s) 586, 1388, 1517
BmrI ACTGGG 1 cut(s) 1129
BmsI GCATC 3 cut(s) 521, 662, 1225
BmuI ACTGGG 1 cut(s) 1129
BpiI GAAGAC 1 cut(s) 459
BpmI CTGGAG 3 cut(s) 1239, 1377, 1499
BpuEI CTTGAG 2 cut(s) 1000, 1009
BpuMI CCSGG 1 cut(s) 586
Bsa29I ATCGAT 1 cut(s) 684
BsaBI GATNNNNATC 1 cut(s) 271
BsaJI CCNNGG 3 cut(s) 408, 759, 1386
BsaWI WCCGGW 1 cut(s) 416
BsaXI ACNNNNNCTCC 2 cut(s) 1375, 1405
Bsc4I CCNNNNNNNGG 5 cut(s) 291, 493, 585, 1133, 1505
Bse1I ACTGG 4 cut(s) 754, 792, 1124, 1394
Bse3DI GCAATG 2 cut(s) 364, 615
Bse8I GATNNNNATC 1 cut(s) 271
BseBI CCWGG 2 cut(s) 1388, 1517
BseCI ATCGAT 1 cut(s) 684
BseDI CCNNGG 3 cut(s) 408, 759, 1386
BseGI GGATG 1 cut(s) 1227
BseJI GATNNNNATC 1 cut(s) 271
BseLI CCNNNNNNNGG 5 cut(s) 291, 493, 585, 1133, 1505
BseMI GCAATG 2 cut(s) 364, 615
BseMII CTCAG 2 cut(s) 267, 719
BseNI ACTGG 4 cut(s) 754, 792, 1124, 1394
BseRI GAGGAG 3 cut(s) 99, 183, 357
BseXI GCAGC 2 cut(s) 184, 683
Bsh1236I CGCG 1 cut(s) 410
Bsh1285I CGRYCG 1 cut(s) 340
BshFI GGCC 3 cut(s) 486, 497, 1438
BshNI GGYRCC 1 cut(s) 413
BshVI ATCGAT 1 cut(s) 684
BsiEI CGRYCG 1 cut(s) 340
BsiHKAI GWGCWC 1 cut(s) 1371
BsiSI CCGG 2 cut(s) 417, 585
BslFI GGGAC 2 cut(s) 1137, 1222
BslI CCNNNNNNNGG 5 cut(s) 291, 493, 585, 1133, 1505
BsmAI GTCTC 2 cut(s) 1413, 1459
BsmFI GGGAC 2 cut(s) 1137, 1222
BsmI GAATGC 1 cut(s) 812
BsnI GGCC 3 cut(s) 486, 497, 1438
Bsp1286I GDGCHC 3 cut(s) 248, 1227, 1371
Bsp143I GATC 5 cut(s) 217, 337, 388, 788, 1599
BspACI CCGC 6 cut(s) 70, 140, 209, 396, 408, 410
BspANI GGCC 3 cut(s) 486, 497, 1438
BspCNI CTCAG 2 cut(s) 266, 718
BspDI ATCGAT 1 cut(s) 684
BspFNI CGCG 1 cut(s) 410
BspLI GGNNCC 6 cut(s) 415, 790, 921, 1108, 1125, 1392
BspMAI CTGCAG 1 cut(s) 1416
BspPI GGATC 5 cut(s) 212, 383, 783, 796, 1607
BspT107I GGYRCC 1 cut(s) 413
BsrDI GCAATG 2 cut(s) 364, 615
BsrI ACTGG 4 cut(s) 754, 792, 1124, 1394
BssECI CCNNGG 3 cut(s) 408, 759, 1386
BssMI GATC 5 cut(s) 217, 337, 388, 788, 1599
BssNAI GTATAC 1 cut(s) 743
BssSI CACGAG 1 cut(s) 299
Bst1107I GTATAC 1 cut(s) 743
Bst2BI CACGAG 1 cut(s) 299
Bst2UI CCWGG 2 cut(s) 1388, 1517
Bst4CI ACNGT 2 cut(s) 371, 1462
Bst6I CTCTTC 2 cut(s) 107, 1358
BstAPI GCANNNNNTGC 3 cut(s) 44, 722, 1244
BstC8I GCNNGC 4 cut(s) 68, 355, 632, 723
BstDEI CTNAG 5 cut(s) 9, 253, 705, 1075, 1433
BstDSI CCRYGG 2 cut(s) 408, 759
BstF5I GGATG 1 cut(s) 1227
BstFNI CGCG 1 cut(s) 410
BstH2I RGCGCY 2 cut(s) 191, 1043
BstHHI GCGC 3 cut(s) 190, 1042, 1298
BstKTI GATC 5 cut(s) 220, 340, 391, 791, 1602
BstMAI GTCTC 2 cut(s) 1413, 1459
BstMBI GATC 5 cut(s) 217, 337, 388, 788, 1599
BstMCI CGRYCG 1 cut(s) 340
BstMWI GCNNNNNNNGC 6 cut(s) 44, 169, 518, 722, 1244, 1388
BstNI CCWGG 2 cut(s) 1388, 1517
BstNSI RCATGY 2 cut(s) 634, 739
BstSCI CCNGG 3 cut(s) 584, 1386, 1515
BstSFI CTRYAG 1 cut(s) 1412
BstUI CGCG 1 cut(s) 410
BstV1I GCAGC 2 cut(s) 184, 683
BstV2I GAAGAC 1 cut(s) 459
BstX2I RGATCY 1 cut(s) 788
BstXI CCANNNNNNTGG 2 cut(s) 1170, 1478
BstYI RGATCY 1 cut(s) 788
BstZ17I GTATAC 1 cut(s) 743
Bsu15I ATCGAT 1 cut(s) 684
BsuRI GGCC 3 cut(s) 486, 497, 1438
BsuTUI ATCGAT 1 cut(s) 684
BtgI CCRYGG 2 cut(s) 408, 759
BtsCI GGATG 1 cut(s) 1227
BtsI GCAGTG 2 cut(s) 1245, 1421
BtsIMutI CAGTG 3 cut(s) 1245, 1318, 1421
Cac8I GCNNGC 4 cut(s) 68, 355, 632, 723
CaiI CAGNNNCTG 1 cut(s) 1244
CfoI GCGC 3 cut(s) 190, 1042, 1298
Cfr13I GGNCC 3 cut(s) 612, 1124, 1519
Cfr42I CCGCGG 1 cut(s) 411
ClaI ATCGAT 1 cut(s) 684
CseI GACGC 1 cut(s) 1566
Csp6I GTAC 3 cut(s) 405, 414, 1117
CviAII CATG 6 cut(s) 276, 631, 692, 717, 736, 1523
CviQI GTAC 3 cut(s) 405, 414, 1117
DdeI CTNAG 5 cut(s) 9, 253, 705, 1075, 1433
DpnI GATC 5 cut(s) 219, 339, 390, 790, 1601
DpnII GATC 5 cut(s) 217, 337, 388, 788, 1599
DraI TTTAAA 1 cut(s) 436
DriI GACNNNNNGTC 1 cut(s) 1428
Eam1104I CTCTTC 2 cut(s) 107, 1358
Eam1105I GACNNNNNGTC 1 cut(s) 1428
EarI CTCTTC 2 cut(s) 107, 1358
EciI GGCGGA 2 cut(s) 155, 198
Ecl136II GAGCTC 1 cut(s) 1369
Eco24I GRGCYC 3 cut(s) 248, 1227, 1371
Eco32I GATATC 1 cut(s) 269
Eco47I GGWCC 3 cut(s) 612, 1124, 1519
Eco47III AGCGCT 1 cut(s) 1041
Eco53kI GAGCTC 1 cut(s) 1369
Eco57I CTGAAG 1 cut(s) 1294
EcoICRI GAGCTC 1 cut(s) 1369
EcoRI GAATTC 2 cut(s) 420, 1200
EcoRII CCWGG 2 cut(s) 1386, 1515
EcoRV GATATC 1 cut(s) 269
EcoT22I ATGCAT 1 cut(s) 814
EcoT38I GRGCYC 3 cut(s) 248, 1227, 1371
FaeI CATG 6 cut(s) 279, 634, 695, 720, 739, 1526
FalI AAGNNNNNCTT 4 cut(s) 68, 100, 898, 930
FaqI GGGAC 2 cut(s) 1137, 1222
FatI CATG 6 cut(s) 275, 630, 691, 716, 735, 1522
FauI CCCGC 1 cut(s) 403
FauNDI CATATG 1 cut(s) 1152
FblI GTMKAC 1 cut(s) 742
Fnu4HI GCNGC 3 cut(s) 173, 397, 672
FokI GGATG 1 cut(s) 1214
FriOI GRGCYC 3 cut(s) 248, 1227, 1371
Fsp4HI GCNGC 3 cut(s) 173, 397, 672
FspBI CTAG 3 cut(s) 393, 936, 1292
GlaI GCGC 3 cut(s) 189, 1041, 1297
GluI GCNGC 3 cut(s) 173, 397, 672
GsuI CTGGAG 3 cut(s) 1239, 1377, 1499
HaeII RGCGCY 2 cut(s) 191, 1043
HaeIII GGCC 3 cut(s) 486, 497, 1438
HapII CCGG 2 cut(s) 417, 585
HgaI GACGC 1 cut(s) 1566
HhaI GCGC 3 cut(s) 190, 1042, 1298
Hin1II CATG 6 cut(s) 279, 634, 695, 720, 739, 1526
Hin6I GCGC 3 cut(s) 188, 1040, 1296
HinP1I GCGC 3 cut(s) 188, 1040, 1296
HincII GTYRAC 1 cut(s) 367
HindII GTYRAC 1 cut(s) 367
HindIII AAGCTT 2 cut(s) 972, 1268
HinfI GANTC 5 cut(s) 143, 257, 526, 953, 1020
HpaII CCGG 2 cut(s) 417, 585
HphI GGTGA 5 cut(s) 252, 274, 492, 659, 1493
Hpy166II GTNNAC 4 cut(s) 52, 282, 367, 743
Hpy188I TCNGA 6 cut(s) 217, 241, 256, 708, 958, 1178
Hpy188III TCNNGA 5 cut(s) 8, 301, 1017, 1026, 1581
Hpy8I GTNNAC 4 cut(s) 52, 282, 367, 743
HpyAV CCTTC 3 cut(s) 497, 1327, 1500
HpyCH4III ACNGT 2 cut(s) 371, 1462
HpyCH4IV ACGT 1 cut(s) 1211
HpyF10VI GCNNNNNNNGC 6 cut(s) 44, 169, 518, 722, 1244, 1388
HpyF3I CTNAG 5 cut(s) 9, 253, 705, 1075, 1433
HpySE526I ACGT 1 cut(s) 1211
Hsp92II CATG 6 cut(s) 279, 634, 695, 720, 739, 1526
HspAI GCGC 3 cut(s) 188, 1040, 1296
KpnI GGTACC 1 cut(s) 417
KspI CCGCGG 1 cut(s) 411
Kzo9I GATC 5 cut(s) 217, 337, 388, 788, 1599
LmnI GCTCC 8 cut(s) 169, 344, 700, 898, 1106, 1366, 1374, 1396
Lsp1109I GCAGC 2 cut(s) 184, 683
LweI GCATC 3 cut(s) 521, 662, 1225
MaeI CTAG 3 cut(s) 393, 936, 1292
MaeII ACGT 1 cut(s) 1211
MaeIII GTNAC 5 cut(s) 527, 961, 1240, 1420, 1555
MalI GATC 5 cut(s) 219, 339, 390, 790, 1601
MboI GATC 5 cut(s) 217, 337, 388, 788, 1599
MboII GAAGA 4 cut(s) 94, 464, 962, 1345
MfeI CAATTG 1 cut(s) 1188
MflI RGATCY 1 cut(s) 788
MhlI GDGCHC 3 cut(s) 248, 1227, 1371
MlyI GAGTC 2 cut(s) 251, 535
MmeI TCCRAC 3 cut(s) 219, 535, 619
Mph1103I ATGCAT 1 cut(s) 814
MslI CAYNNNNRTG 1 cut(s) 134
MspA1I CMGCKG 1 cut(s) 410
MspI CCGG 2 cut(s) 417, 585
MspR9I CCNGG 3 cut(s) 586, 1388, 1517
MunI CAATTG 1 cut(s) 1188
Mva1269I GAATGC 1 cut(s) 812
MvaI CCWGG 2 cut(s) 1388, 1517
MvnI CGCG 1 cut(s) 410
MwoI GCNNNNNNNGC 6 cut(s) 44, 169, 518, 722, 1244, 1388
NciI CCSGG 1 cut(s) 586
NdeI CATATG 1 cut(s) 1152
NdeII GATC 5 cut(s) 217, 337, 388, 788, 1599
NlaIII CATG 6 cut(s) 279, 634, 695, 720, 739, 1526
NlaIV GGNNCC 6 cut(s) 415, 790, 921, 1108, 1125, 1392
NmuCI GTSAC 5 cut(s) 527, 961, 1240, 1420, 1555
NsiI ATGCAT 1 cut(s) 814
NspI RCATGY 2 cut(s) 634, 739
PaeI GCATGC 1 cut(s) 634
PciI ACATGT 1 cut(s) 735
PctI GAATGC 1 cut(s) 812
PfeI GAWTC 3 cut(s) 143, 953, 1020
PflMI CCANNNNNTGG 1 cut(s) 1133
PfoI TCCNGGA 1 cut(s) 1515
PkrI GCNGC 3 cut(s) 174, 398, 673
Ple19I CGATCG 1 cut(s) 340
PleI GAGTC 2 cut(s) 251, 534
PpsI GAGTC 2 cut(s) 251, 534
PscI ACATGT 1 cut(s) 735
PshBI ATTAAT 1 cut(s) 327
PsiI TTATAA 1 cut(s) 1257
Psp124BI GAGCTC 1 cut(s) 1371
Psp6I CCWGG 2 cut(s) 1386, 1515
PspGI CCWGG 2 cut(s) 1386, 1515
PspN4I GGNNCC 6 cut(s) 415, 790, 921, 1108, 1125, 1392
PspPI GGNCC 3 cut(s) 612, 1124, 1519
PstI CTGCAG 1 cut(s) 1416
PstNI CAGNNNCTG 1 cut(s) 1244
PsuI RGATCY 1 cut(s) 788
PvuI CGATCG 1 cut(s) 340
RsaI GTAC 3 cut(s) 406, 415, 1118
RsaNI GTAC 3 cut(s) 405, 414, 1117
RseI CAYNNNNRTG 1 cut(s) 134
SacI GAGCTC 1 cut(s) 1371
SacII CCGCGG 1 cut(s) 411
SatI GCNGC 3 cut(s) 173, 397, 672
Sau3AI GATC 5 cut(s) 217, 337, 388, 788, 1599
Sau96I GGNCC 3 cut(s) 612, 1124, 1519
SchI GAGTC 2 cut(s) 251, 535
ScrFI CCNGG 3 cut(s) 586, 1388, 1517
SduI GDGCHC 3 cut(s) 248, 1227, 1371
SfaNI GCATC 3 cut(s) 521, 662, 1225
SfcI CTRYAG 1 cut(s) 1412
Sfr303I CCGCGG 1 cut(s) 411
SgrBI CCGCGG 1 cut(s) 411
SinI GGWCC 3 cut(s) 612, 1124, 1519
SmiMI CAYNNNNRTG 1 cut(s) 134
SmlI CTYRAG 2 cut(s) 1015, 1024
SmoI CTYRAG 2 cut(s) 1015, 1024
SpeI ACTAGT 1 cut(s) 935
SphI GCATGC 1 cut(s) 634
SsiI CCGC 6 cut(s) 70, 140, 209, 396, 408, 410
SspI AATATT 1 cut(s) 1302
SspMI CTAG 3 cut(s) 393, 936, 1292
SstI GAGCTC 1 cut(s) 1371
StyD4I CCNGG 3 cut(s) 584, 1386, 1515
TaaI ACNGT 2 cut(s) 371, 1462
TaiI ACGT 1 cut(s) 1214
TaqI TCGA 5 cut(s) 265, 336, 340, 684, 1050
TauI GCSGC 1 cut(s) 399
TfiI GAWTC 3 cut(s) 143, 953, 1020
TscAI CASTG 3 cut(s) 1245, 1318, 1421
TseFI GTSAC 5 cut(s) 527, 961, 1240, 1420, 1555
TseI GCWGC 2 cut(s) 172, 671
Tsp45I GTSAC 5 cut(s) 527, 961, 1240, 1420, 1555
TspDTI ATGAA 6 cut(s) 649, 670, 881, 1049, 1169, 1582
TspRI CASTG 3 cut(s) 1245, 1318, 1421
Van91I CCANNNNNTGG 1 cut(s) 1133
VpaK11BI GGWCC 3 cut(s) 612, 1124, 1519
VspI ATTAAT 1 cut(s) 327
XapI RAATTY 2 cut(s) 420, 1200
XceI RCATGY 2 cut(s) 634, 739
XmiI GTMKAC 1 cut(s) 742
XspI CTAG 3 cut(s) 393, 936, 1292
Zsp2I ATGCAT 1 cut(s) 814
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.