pycom2749g00050

May participate in a complex which severs microtubules in an ATP-dependent manner. Microtubule severing may promote rapid reorganization of cellular microtubule arrays

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00002749
OG0001097
Physical Location & Seq
Forward (+)
26008 .. 26677
670 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 330 bp
ATGACTACTAAACGCGCTTACAAGCTACAGGAATTTGTAGCACATTCTGCAGCTGTGAATTGCCTCAAGATTGGCAGAAAGTCTTCTAGAGTTCTTGTCACCGGCGGAGAAGATCACAAGGTCAATCTCTGGGCCATTGGGAAACCCAATGCCTTACTGAGTTTGTCTGGACACACCAGTGGAATTGATTCAGTAAGCTTTGATTCTTCAGAGGTCTTAGTAGCTGCAGGAGCTGCAAGTGGTACAATTAAGCTTTGGGATTTAGAGGAGGCAAAGAGTATGCGGTTTATTTATTTTATTTTATATTTTTTCTGCAGTATCTTCCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0031461 GO:0032991 GO:0044424 GO:0044464 GO:0080008 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Katanin_con80 WD40

Protein Analysis

110

Amino Acids

11.93

Weight (kDa)

9.0

Isoelectric Point (pI)

30.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR5 PF25175 8 - 97 1.6e-14 WDR5 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 9 - 94 1e-07 TEP-1 second beta-propeller
Beta-prop_WDR3_1st PF25173 10 - 94 7.3e-14 WDR3 first beta-propeller domain
WD40_Gbeta PF25391 11 - 92 2.9e-06 G protein beta WD-40 repeat protein
WDR55 PF24796 13 - 92 9.2e-06 WDR55
Beta-prop_SCAP PF24017 14 - 88 4.3e-08 SCAP Beta-propeller
WD40_Prp19 PF24814 15 - 94 4.1e-12 Prp19 WD40 domain
WD40_WDHD1_1st PF24817 15 - 95 7.9e-12 WDHD1 first WD40 domain
EIF3I PF24805 15 - 95 5.2e-06 EIF3I
Beta-prop_EML_2 PF23414 28 - 93 6.1e-06 Echinoderm microtubule-associated protein second beta-propeller
WD40 PF00400 52 - 87 1.5e-07 WD domain, G-beta repeat
Beta-prop_THOC3 PF25174 52 - 97 1.3e-06 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 15
AciI CCGC 2 cut(s) 105, 283
AcsI RAATTY 1 cut(s) 32
AcuI CTGAAG 1 cut(s) 192
AfaI GTAC 1 cut(s) 244
AleI CACNNNNGTG 1 cut(s) 177
AluBI AGCT 6 cut(s) 25, 53, 198, 224, 233, 253
AluI AGCT 6 cut(s) 25, 53, 198, 224, 233, 253
AlwNI CAGNNNCTG 1 cut(s) 233
AoxI GGCC 1 cut(s) 132
ApeKI GCWGC 3 cut(s) 50, 224, 233
ApoI RAATTY 1 cut(s) 32
Asp700I GAANNNNTTC 2 cut(s) 82, 187
AspLEI GCGC 1 cut(s) 17
AspS9I GGNCC 1 cut(s) 132
AsuHPI GGTGA 1 cut(s) 91
BbsI GAAGAC 1 cut(s) 75
BbvI GCAGC 3 cut(s) 62, 211, 220
BfaI CTAG 1 cut(s) 87
BfmI CTRYAG 4 cut(s) 26, 48, 225, 313
BisI GCNGC 3 cut(s) 51, 225, 234
BlsI GCNGC 3 cut(s) 52, 226, 235
BmgT120I GGNCC 1 cut(s) 132
BpiI GAAGAC 1 cut(s) 75
BpuEI CTTGAG 1 cut(s) 50
Bse118I RCCGGY 1 cut(s) 101
Bse1I ACTGG 1 cut(s) 177
BseMII CTCAG 1 cut(s) 149
BseNI ACTGG 1 cut(s) 177
BseRI GAGGAG 1 cut(s) 281
BseXI GCAGC 3 cut(s) 62, 211, 220
Bsh1236I CGCG 1 cut(s) 15
BshFI GGCC 1 cut(s) 134
BsiSI CCGG 1 cut(s) 102
BsnI GGCC 1 cut(s) 134
Bsp143I GATC 1 cut(s) 112
BspACI CCGC 2 cut(s) 105, 283
BspANI GGCC 1 cut(s) 134
BspCNI CTCAG 1 cut(s) 150
BspFNI CGCG 1 cut(s) 15
BspMAI CTGCAG 3 cut(s) 52, 229, 317
BsrFI RCCGGY 1 cut(s) 101
BsrI ACTGG 1 cut(s) 177
BssAI RCCGGY 1 cut(s) 101
BssMI GATC 1 cut(s) 112
BstAPI GCANNNNNTGC 2 cut(s) 47, 233
BstDEI CTNAG 2 cut(s) 158, 217
BstFNI CGCG 1 cut(s) 15
BstHHI GCGC 1 cut(s) 17
BstKTI GATC 1 cut(s) 115
BstMBI GATC 1 cut(s) 112
BstMWI GCNNNNNNNGC 3 cut(s) 47, 230, 233
BstSFI CTRYAG 4 cut(s) 26, 48, 225, 313
BstUI CGCG 1 cut(s) 15
BstV1I GCAGC 3 cut(s) 62, 211, 220
BstV2I GAAGAC 1 cut(s) 75
BsuRI GGCC 1 cut(s) 134
BtsIMutI CAGTG 1 cut(s) 184
CaiI CAGNNNCTG 1 cut(s) 233
CfoI GCGC 1 cut(s) 17
Cfr10I RCCGGY 1 cut(s) 101
Cfr13I GGNCC 1 cut(s) 132
Csp6I GTAC 1 cut(s) 243
CviJI RGCY 7 cut(s) 25, 53, 134, 198, 224, 233, 253
CviKI_1 RGCY 7 cut(s) 25, 53, 134, 198, 224, 233, 253
CviQI GTAC 1 cut(s) 243
DdeI CTNAG 2 cut(s) 158, 217
DpnI GATC 1 cut(s) 114
DpnII GATC 1 cut(s) 112
EciI GGCGGA 1 cut(s) 120
Eco57I CTGAAG 1 cut(s) 192
FaiI YATR 2 cut(s) 281, 304
Fnu4HI GCNGC 3 cut(s) 51, 225, 234
Fsp4HI GCNGC 3 cut(s) 51, 225, 234
FspBI CTAG 1 cut(s) 87
GlaI GCGC 1 cut(s) 16
GluI GCNGC 3 cut(s) 51, 225, 234
HaeIII GGCC 1 cut(s) 134
HapII CCGG 1 cut(s) 102
HhaI GCGC 1 cut(s) 17
Hin6I GCGC 1 cut(s) 15
HinP1I GCGC 1 cut(s) 15
HindIII AAGCTT 2 cut(s) 196, 251
HinfI GANTC 2 cut(s) 188, 203
HpaII CCGG 1 cut(s) 102
HphI GGTGA 1 cut(s) 91
Hpy188I TCNGA 1 cut(s) 211
Hpy188III TCNNGA 3 cut(s) 67, 87, 168
HpyCH4V TGCA 4 cut(s) 50, 227, 236, 315
HpyF10VI GCNNNNNNNGC 3 cut(s) 47, 230, 233
HpyF3I CTNAG 2 cut(s) 158, 217
HspAI GCGC 1 cut(s) 15
Kzo9I GATC 1 cut(s) 112
LmnI GCTCC 1 cut(s) 230
LpnPI CCDG 6 cut(s) 14, 115, 115, 153, 190, 213
Lsp1109I GCAGC 3 cut(s) 62, 211, 220
MaeI CTAG 1 cut(s) 87
MaeIII GTNAC 1 cut(s) 97
MalI GATC 1 cut(s) 114
MboI GATC 1 cut(s) 112
MboII GAAGA 4 cut(s) 75, 122, 198, 313
MluCI AATT 4 cut(s) 32, 58, 183, 246
MnlI CCTC 4 cut(s) 74, 205, 259, 262
MroXI GAANNNNTTC 2 cut(s) 82, 187
MseI TTAA 2 cut(s) 249, 328
MslI CAYNNNNRTG 1 cut(s) 177
MspA1I CMGCKG 1 cut(s) 53
MspI CCGG 1 cut(s) 102
MvnI CGCG 1 cut(s) 15
MwoI GCNNNNNNNGC 3 cut(s) 47, 230, 233
NdeII GATC 1 cut(s) 112
NmuCI GTSAC 1 cut(s) 97
OliI CACNNNNGTG 1 cut(s) 177
PdmI GAANNNNTTC 2 cut(s) 82, 187
PfeI GAWTC 2 cut(s) 188, 203
PkrI GCNGC 3 cut(s) 52, 226, 235
PspPI GGNCC 1 cut(s) 132
PstI CTGCAG 3 cut(s) 52, 229, 317
PstNI CAGNNNCTG 1 cut(s) 233
PvuII CAGCTG 1 cut(s) 53
RsaI GTAC 1 cut(s) 244
RsaNI GTAC 1 cut(s) 243
RseI CAYNNNNRTG 1 cut(s) 177
SaqAI TTAA 2 cut(s) 249, 328
SatI GCNGC 3 cut(s) 51, 225, 234
Sau3AI GATC 1 cut(s) 112
Sau96I GGNCC 1 cut(s) 132
SetI ASST 8 cut(s) 27, 55, 123, 200, 216, 226, 235, 255
SfcI CTRYAG 4 cut(s) 26, 48, 225, 313
SgrAI CRCCGGYG 1 cut(s) 101
SmiMI CAYNNNNRTG 1 cut(s) 177
SmlI CTYRAG 1 cut(s) 65
SmoI CTYRAG 1 cut(s) 65
Sse9I AATT 4 cut(s) 32, 58, 183, 246
SsiI CCGC 2 cut(s) 105, 283
SspMI CTAG 1 cut(s) 87
TasI AATT 4 cut(s) 32, 58, 183, 246
TfiI GAWTC 2 cut(s) 188, 203
Tru1I TTAA 2 cut(s) 249, 328
Tru9I TTAA 2 cut(s) 249, 328
TscAI CASTG 1 cut(s) 184
TseFI GTSAC 1 cut(s) 97
TseI GCWGC 3 cut(s) 50, 224, 233
Tsp45I GTSAC 1 cut(s) 97
TspRI CASTG 1 cut(s) 184
XapI RAATTY 1 cut(s) 32
XbaI TCTAGA 1 cut(s) 86
XmnI GAANNNNTTC 2 cut(s) 82, 187
XspI CTAG 1 cut(s) 87
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.