Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0000867

Physical Location & Seq

Genomic Coordinates Reverse (-)

15977221 .. 15979820

Gene Structure 2600 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1440 bp

ATGGGTTCAACACAACTAGGAGCCAAAGAACTCCATGCAGTCTGTCTCCCTTTCCCAGCACAAGGCCATGTTAACCCTATGATGCAATTAGCCAAGCTTCTGTATTCCAGGGGATTCCAGATAACCTTCGTCAACACCGAGTTCAACCACCGGCGTTTAATCCGGTCCAGAGGCTCTGATTCCGTCAAGGGACTACCGGACTTCCAATTTGCGACCATACCGGACGGGCTGCCACCTTCAGATAAAGATGCAACCCAAGATGTTCCAACTCTATGCGACTCCATCAGGAAAACATGTCTAGGCCCTTTCAAGGAGCTGGTGACTAAACGCAATTCCTCGTCTGAAGTGCCACATATTACTTGTATAGTTTCTGACGGGATCATGGGATTTGGGAGGGAAGTTGCTAAGGAAATGGGGATTCCTGGGGTTCAGCTTTGGACTTCTTCTGCTTGTGGCTTCATGGGGTACCTGCAATACGGCGAACTCGTCAAACGAGGCATTGTTCCATTCAAAGATGAAAGTTTCATGCAAGATGGTACACTCGATGAGCCAATTGATTGGATCCCAGGCATGAGGAATATGAGGCTCAAGGACATCCCTAGCTTTATCAGAGTCACTAATGTCAATGATATAATGTTCGACTACTTGAGATCTGAAGCACAACACTGCTTGAAATCCTCTGCAATCATCTTCAACACGTTTGACGAATTGGAGCATGAAGTGTTAGAAGAAATCTCAGCCATGTTTCCCAACATTTACACAATAGGTCCACTTGCTTTGCTTGGTAGGCATGGGCCCGAAAGCAATTTGGTCAAGTCCCTAAGCTCAGGCTTATGGAAAGAAGACGCAAAATGCATCGAATGGCTTGACAAACAGAAACCTGATTCGGTTGTGTATGTGAATTATGGGAGCATAGCTATGATGACAGACCAACATTTGAAAGAATTTGCATGGGGACTTGCAAATAGCAAGCACCATTTTTTGTGGATTGTCAGGCCTGATGTTGTAAAAGGTGATTCGGTAGTTTTACCCGAAGAATTTTTTGAGGAGATAAAGGATAGGGGTTACGTAACAAGTTGGTGCCCACAGGAGCAAGTGCTAGCACATCCATCAGTTGGGGTTTTTCTAACACATTGTGGTTGGAATTCTACGATTGAAACTATATCGGAGGGAGTGCCTGTACTTTGCTGGCCTTTCCGTTCTGAGCAACTTACAAATTGCCGTTTCCTGTGTACTGATTGGGGAATTGGTATGGAGGTAAACAATGATGTTAAGTGCAATGACATTGAAGCACTTGTTAAGGAAATGCTGGAAGGGGAGAAAGGCATGAAGATGAGGCGAAAGGCAGAGGAATGGAAGAAGAAAGCAATAGCAGCTACTGACACTGGAGGATCATCGTACAATAATTTTGAGAGACTGATTAAGGTGGCTCTTCAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0008194 GO:0015020 GO:0016740 GO:0016757 GO:0016758 GO:0035251 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0046527 GO:0050403 GO:0050502 GO:0080043 GO:0080044

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

UDPGT

Protein Analysis

479

Amino Acids

53.95

Weight (kDa)

5.64

Isoelectric Point (pI)

41.27

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_transf_N	PF26168	13 - 60	4.4e-08	Glycosyltransferase, N-terminal domain
UDPGT	PF00201	174 - 440	1.4e-23	UDP-glucoronosyl and UDP-glucosyl transferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000867)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g35790 FvH4_6g52580 FvH4_7g03840
malus_domestica	MD00G1052900.v1.1 MD01G1029200.v1.1 MD01G1033000.v1.1 MD01G1033200.v1.1 MD11G1245400.v1.1 MD11G1245700.v1.1 MD11G1246000.v1.1 MD13G1080500.v1.1
prunus_persica	Prupe.1G266300_v2.0.a1 Prupe.6G189800_v2.0.a1 Prupe.6G189900_v2.0.a1 Prupe.6G190100_v2.0.a1 Prupe.6G190300_v2.0.a1 Prupe.6G190600_v2.0.a1
pyrus_communis	pycom01g05760 pycom01g06000 pycom11g21770
rosa_chinensis	RchiOBHm_Chr1g0327231 RchiOBHm_Chr2g0112881 RchiOBHm_Chr2g0112891 RchiOBHm_Chr2g0112921 RchiOBHm_Chr2g0112941 RchiOBHm_Chr2g0113001 RchiOBHm_Chr2g0113011 RchiOBHm_Chr4g0445311 RchiOBHm_Chr6g0267321
rosa_laevigata	RLG00000005749 RLG00000018018 RLG00000018020 RLG00000018023 RLG00000018027 RLG00000018029 RLG00000018030 RLG00000030033
rosa_multiflora	Rmu_sc0000050.1_g000021 Rmu_sc0000795.1_g000117 Rmu_sc0002351.1_g000078 Rmu_sc0002351.1_g000084 Rmu_sc0002351.1_g000093 Rmu_sc0002351.1_g000104 Rmu_sc0002418.1_g000002 Rmu_sc0014256.1_g000002 Rmu_sc0014256.1_g000005 Rmu_sc0024545.1_g000001
rosa_roxburghii	Rroxscaffold_2G00130960 Rroxscaffold_2G00131000 Rroxscaffold_2G00131090 Rroxscaffold_5G00385830
rosa_rugosa	Rorug01G0064700 Rorug02G0183200 Rorug02G0183200 Rorug04G0360300
rosa_samantha	Rh1AG081700 Rh2BG251400 Rh2BG251900 Rh4CG448400
rosa_wichuraiana	Rw0G006830 Rw1G006460 Rw1G015720 Rw2G018630 Rw2G018640 Rw2G018660 Rw2G018690 Rw4G036170

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	477
Acc65I	GGTACC	1 cut(s)	465
AccB1I	GGYRCC	2 cut(s)	465, 1080
AccB7I	CCANNNNNTGG	1 cut(s)	1115
AclWI	GGATC	4 cut(s)	386, 556, 569, 1399
AcsI	RAATTY	3 cut(s)	944, 1037, 1144
AcuI	CTGAAG	3 cut(s)	222, 363, 675
AdeI	CACNNNGTG	1 cut(s)	1136
AfaI	GTAC	5 cut(s)	467, 538, 1182, 1234, 1400
AfiI	CCNNNNNNNGG	3 cut(s)	62, 310, 1115
AflIII	ACRYGT	2 cut(s)	293, 696
AjnI	CCWGG	3 cut(s)	107, 421, 565
AluBI	AGCT	7 cut(s)	97, 316, 433, 603, 825, 917, 1376
AluI	AGCT	7 cut(s)	97, 316, 433, 603, 825, 917, 1376
Alw26I	GTCTC	2 cut(s)	50, 1408
AlwI	GGATC	4 cut(s)	386, 556, 569, 1399
AlwNI	CAGNNNCTG	1 cut(s)	1379
AoxI	GGCC	5 cut(s)	64, 301, 794, 995, 1190
ApaI	GGGCCC	1 cut(s)	798
ApeKI	GCWGC	2 cut(s)	229, 1373
ApoI	RAATTY	3 cut(s)	944, 1037, 1144
ArsI	GACNNNNNNTTYG	2 cut(s)	191, 223
Asp718I	GGTACC	1 cut(s)	465
AspS9I	GGNCC	5 cut(s)	165, 302, 767, 794, 795
AsuHPI	GGTGA	2 cut(s)	331, 1025
AsuNHI	GCTAGC	1 cut(s)	1099
AvaII	GGWCC	2 cut(s)	165, 767
BaeGI	GKGCMC	2 cut(s)	798, 1085
BamHI	GGATCC	1 cut(s)	561
BanI	GGYRCC	2 cut(s)	465, 1080
BanII	GRGCYC	1 cut(s)	798
BbsI	GAAGAC	1 cut(s)	849
BbvI	GCAGC	2 cut(s)	216, 1385
BccI	CCATC	3 cut(s)	290, 527, 1117
BceAI	ACGGC	2 cut(s)	493, 1206
BciT130I	CCWGG	3 cut(s)	109, 423, 567
BcoDI	GTCTC	2 cut(s)	50, 1408
BfaI	CTAG	4 cut(s)	17, 299, 600, 1100
BfuAI	ACCTGC	1 cut(s)	477
BglII	AGATCT	1 cut(s)	650
BisI	GCNGC	2 cut(s)	230, 1374
BlsI	GCNGC	2 cut(s)	231, 1375
Bme1390I	CCNGG	3 cut(s)	109, 423, 567
Bme18I	GGWCC	2 cut(s)	165, 767
BmgT120I	GGNCC	5 cut(s)	165, 302, 767, 794, 795
BmiI	GGNNCC	5 cut(s)	22, 467, 563, 796, 1082
BmrFI	CCNGG	3 cut(s)	109, 423, 567
BmsI	GCATC	3 cut(s)	72, 238, 864
BmtI	GCTAGC	1 cut(s)	1103
BpiI	GAAGAC	1 cut(s)	849
BpmI	CTGGAG	1 cut(s)	1407
Bpu10I	CCTNAGC	3 cut(s)	405, 821, 826
BpuEI	CTTGAG	2 cut(s)	572, 667
BsaAI	YACGTR	1 cut(s)	1069
BsaJI	CCNNGG	3 cut(s)	108, 422, 565
BsaWI	WCCGGW	3 cut(s)	162, 196, 220
BsaXI	ACNNNNNCTCC	2 cut(s)	1164, 1194
Bsc4I	CCNNNNNNNGG	3 cut(s)	62, 310, 1115
Bse118I	RCCGGY	1 cut(s)	150
Bse1I	ACTGG	1 cut(s)	1390
Bse3DI	GCAATG	1 cut(s)	1285
BseBI	CCWGG	3 cut(s)	109, 423, 567
BseDI	CCNNGG	3 cut(s)	108, 422, 565
BseGI	GGATG	2 cut(s)	594, 1105
BseLI	CCNNNNNNNGG	3 cut(s)	62, 310, 1115
BseMI	GCAATG	1 cut(s)	1285
BseMII	CTCAG	3 cut(s)	750, 840, 1194
BseNI	ACTGG	1 cut(s)	1390
BseRI	GAGGAG	1 cut(s)	1061
BseSI	GKGCMC	2 cut(s)	798, 1085
BseXI	GCAGC	2 cut(s)	216, 1385
BseYI	CCCAGC	1 cut(s)	55
BshFI	GGCC	5 cut(s)	66, 303, 796, 997, 1192
BshNI	GGYRCC	2 cut(s)	465, 1080
BsiSI	CCGG	4 cut(s)	151, 163, 197, 221
BslFI	GGGAC	3 cut(s)	204, 802, 969
BslI	CCNNNNNNNGG	3 cut(s)	62, 310, 1115
BsmAI	GTCTC	2 cut(s)	50, 1408
BsmFI	GGGAC	3 cut(s)	204, 802, 969
BsnI	GGCC	5 cut(s)	66, 303, 796, 997, 1192
Bsp120I	GGGCCC	1 cut(s)	794
Bsp1286I	GDGCHC	2 cut(s)	798, 1085
Bsp143I	GATC	4 cut(s)	378, 561, 650, 1391
BspANI	GGCC	5 cut(s)	66, 303, 796, 997, 1192
BspCNI	CTCAG	3 cut(s)	749, 839, 1195
BspLI	GGNNCC	5 cut(s)	22, 467, 563, 796, 1082
BspMI	ACCTGC	1 cut(s)	477
BspOI	GCTAGC	1 cut(s)	1103
BspPI	GGATC	4 cut(s)	386, 556, 569, 1399
BspQI	GCTCTTC	1 cut(s)	1437
BspT107I	GGYRCC	2 cut(s)	465, 1080
BsrDI	GCAATG	1 cut(s)	1285
BsrFI	RCCGGY	1 cut(s)	150
BsrI	ACTGG	1 cut(s)	1390
BssAI	RCCGGY	1 cut(s)	150
BssECI	CCNNGG	3 cut(s)	108, 422, 565
BssMI	GATC	4 cut(s)	378, 561, 650, 1391
Bst2UI	CCWGG	3 cut(s)	109, 423, 567
Bst6I	CTCTTC	1 cut(s)	1437
BstBAI	YACGTR	1 cut(s)	1069
BstC8I	GCNNGC	3 cut(s)	971, 1101, 1190
BstDEI	CTNAG	5 cut(s)	405, 736, 821, 826, 1203
BstF5I	GGATG	2 cut(s)	594, 1105
BstKTI	GATC	4 cut(s)	381, 564, 653, 1394
BstMAI	GTCTC	2 cut(s)	50, 1408
BstMBI	GATC	4 cut(s)	378, 561, 650, 1391
BstMWI	GCNNNNNNNGC	2 cut(s)	787, 1373
BstNI	CCWGG	3 cut(s)	109, 423, 567
BstNSI	RCATGY	1 cut(s)	297
BstSCI	CCNGG	3 cut(s)	107, 421, 565
BstSLI	GKGCMC	2 cut(s)	798, 1085
BstSNI	TACGTA	1 cut(s)	1069
BstV1I	GCAGC	2 cut(s)	216, 1385
BstV2I	GAAGAC	1 cut(s)	849
BstX2I	RGATCY	2 cut(s)	561, 650
BstXI	CCANNNNNNTGG	1 cut(s)	558
BstYI	RGATCY	2 cut(s)	561, 650
BsuRI	GGCC	5 cut(s)	66, 303, 796, 997, 1192
BtsCI	GGATG	2 cut(s)	594, 1105
BtsI	GCAGTG	1 cut(s)	664
BtsIMutI	CAGTG	2 cut(s)	664, 1383
BveI	ACCTGC	1 cut(s)	477
Cac8I	GCNNGC	3 cut(s)	971, 1101, 1190
CaiI	CAGNNNCTG	1 cut(s)	1379
Cfr10I	RCCGGY	1 cut(s)	150
Cfr13I	GGNCC	5 cut(s)	165, 302, 767, 794, 795
CseI	GACGC	1 cut(s)	854
Csp6I	GTAC	5 cut(s)	466, 537, 1181, 1233, 1399
CspCI	CAANNNNNGTGG	2 cut(s)	759, 794
CviQI	GTAC	5 cut(s)	466, 537, 1181, 1233, 1399
DdeI	CTNAG	5 cut(s)	405, 736, 821, 826, 1203
DpnI	GATC	4 cut(s)	380, 563, 652, 1393
DpnII	GATC	4 cut(s)	378, 561, 650, 1391
DraIII	CACNNNGTG	1 cut(s)	1136
Eam1104I	CTCTTC	1 cut(s)	1437
EarI	CTCTTC	1 cut(s)	1437
Eco105I	TACGTA	1 cut(s)	1069
Eco147I	AGGCCT	1 cut(s)	997
Eco24I	GRGCYC	1 cut(s)	798
Eco47I	GGWCC	2 cut(s)	165, 767
Eco57I	CTGAAG	3 cut(s)	222, 363, 675
EcoO109I	RGGNCCY	1 cut(s)	302
EcoRI	GAATTC	1 cut(s)	1144
EcoRII	CCWGG	3 cut(s)	107, 421, 565
EcoT22I	ATGCAT	1 cut(s)	857
EcoT38I	GRGCYC	1 cut(s)	798
FaqI	GGGAC	3 cut(s)	204, 802, 969
Fnu4HI	GCNGC	2 cut(s)	230, 1374
FokI	GGATG	2 cut(s)	581, 1092
FriOI	GRGCYC	1 cut(s)	798
Fsp4HI	GCNGC	2 cut(s)	230, 1374
FspBI	CTAG	4 cut(s)	17, 299, 600, 1100
GluI	GCNGC	2 cut(s)	230, 1374
GsaI	CCCAGC	1 cut(s)	59
GsuI	CTGGAG	1 cut(s)	1407
HaeIII	GGCC	5 cut(s)	66, 303, 796, 997, 1192
HapII	CCGG	4 cut(s)	151, 163, 197, 221
HgaI	GACGC	1 cut(s)	854
HincII	GTYRAC	2 cut(s)	73, 133
HindII	GTYRAC	2 cut(s)	73, 133
HindIII	AAGCTT	1 cut(s)	95
HinfI	GANTC	7 cut(s)	114, 179, 278, 418, 612, 884, 1016
HpaI	GTTAAC	1 cut(s)	73
HpaII	CCGG	4 cut(s)	151, 163, 197, 221
HphI	GGTGA	2 cut(s)	331, 1025
Hpy166II	GTNNAC	6 cut(s)	73, 133, 539, 770, 1233, 1261
Hpy188I	TCNGA	8 cut(s)	178, 241, 343, 373, 611, 655, 1168, 1204
Hpy188III	TCNNGA	3 cut(s)	118, 168, 286
Hpy8I	GTNNAC	6 cut(s)	73, 133, 539, 770, 1233, 1261
HpyAV	CCTTC	3 cut(s)	136, 246, 1307
HpyCH4IV	ACGT	2 cut(s)	698, 1068
HpyF10VI	GCNNNNNNNGC	2 cut(s)	787, 1373
HpyF3I	CTNAG	5 cut(s)	405, 736, 821, 826, 1203
HpySE526I	ACGT	2 cut(s)	698, 1068
KpnI	GGTACC	1 cut(s)	469
KspAI	GTTAAC	1 cut(s)	73
Kzo9I	GATC	4 cut(s)	378, 561, 650, 1391
LguI	GCTCTTC	1 cut(s)	1437
LmnI	GCTCC	5 cut(s)	20, 313, 712, 909, 1090
Lsp1109I	GCAGC	2 cut(s)	216, 1385
LweI	GCATC	3 cut(s)	72, 238, 864
MaeI	CTAG	4 cut(s)	17, 299, 600, 1100
MaeII	ACGT	2 cut(s)	698, 1068
MaeIII	GTNAC	4 cut(s)	319, 613, 1064, 1069
MalI	GATC	4 cut(s)	380, 563, 652, 1393
MboI	GATC	4 cut(s)	378, 561, 650, 1391
MboII	GAAGA	9 cut(s)	435, 682, 740, 854, 1046, 1342, 1369, 1372, 1424
MfeI	CAATTG	1 cut(s)	552
MflI	RGATCY	2 cut(s)	561, 650
MhlI	GDGCHC	2 cut(s)	798, 1085
MlyI	GAGTC	2 cut(s)	272, 621
MmeI	TCCRAC	2 cut(s)	290, 1121
Mph1103I	ATGCAT	1 cut(s)	857
MseI	TTAA	5 cut(s)	72, 158, 1272, 1299, 1422
MslI	CAYNNNNRTG	2 cut(s)	917, 1331
MspI	CCGG	4 cut(s)	151, 163, 197, 221
MspR9I	CCNGG	3 cut(s)	109, 423, 567
MunI	CAATTG	1 cut(s)	552
MvaI	CCWGG	3 cut(s)	109, 423, 567
MwoI	GCNNNNNNNGC	2 cut(s)	787, 1373
NdeII	GATC	4 cut(s)	378, 561, 650, 1391
NheI	GCTAGC	1 cut(s)	1099
NlaIV	GGNNCC	5 cut(s)	22, 467, 563, 796, 1082
NmuCI	GTSAC	2 cut(s)	319, 613
NsiI	ATGCAT	1 cut(s)	857
NspI	RCATGY	1 cut(s)	297
PceI	AGGCCT	1 cut(s)	997
PciI	ACATGT	1 cut(s)	293
PciSI	GCTCTTC	1 cut(s)	1437
PcsI	WCGNNNNNNNCGW	2 cut(s)	135, 483
PfeI	GAWTC	5 cut(s)	114, 179, 418, 884, 1016
PflMI	CCANNNNNTGG	1 cut(s)	1115
PkrI	GCNGC	2 cut(s)	231, 1375
PleI	GAGTC	2 cut(s)	272, 620
PpsI	GAGTC	2 cut(s)	272, 620
Ppu21I	YACGTR	1 cut(s)	1069
PscI	ACATGT	1 cut(s)	293
Psp6I	CCWGG	3 cut(s)	107, 421, 565
PspFI	CCCAGC	1 cut(s)	55
PspGI	CCWGG	3 cut(s)	107, 421, 565
PspN4I	GGNNCC	5 cut(s)	22, 467, 563, 796, 1082
PspOMI	GGGCCC	1 cut(s)	794
PspPI	GGNCC	5 cut(s)	165, 302, 767, 794, 795
PstNI	CAGNNNCTG	1 cut(s)	1379
PsuI	RGATCY	2 cut(s)	561, 650
RsaI	GTAC	5 cut(s)	467, 538, 1182, 1234, 1400
RsaNI	GTAC	5 cut(s)	466, 537, 1181, 1233, 1399
RseI	CAYNNNNRTG	2 cut(s)	917, 1331
SapI	GCTCTTC	1 cut(s)	1437
SaqAI	TTAA	5 cut(s)	72, 158, 1272, 1299, 1422
SatI	GCNGC	2 cut(s)	230, 1374
Sau3AI	GATC	4 cut(s)	378, 561, 650, 1391
Sau96I	GGNCC	5 cut(s)	165, 302, 767, 794, 795
SchI	GAGTC	2 cut(s)	272, 621
ScrFI	CCNGG	3 cut(s)	109, 423, 567
SduI	GDGCHC	2 cut(s)	798, 1085
SfaNI	GCATC	3 cut(s)	72, 238, 864
SgrAI	CRCCGGYG	1 cut(s)	150
SinI	GGWCC	2 cut(s)	165, 767
SmiMI	CAYNNNNRTG	2 cut(s)	917, 1331
SmlI	CTYRAG	2 cut(s)	587, 646
SmoI	CTYRAG	2 cut(s)	587, 646
SnaBI	TACGTA	1 cut(s)	1069
SseBI	AGGCCT	1 cut(s)	997
SspMI	CTAG	4 cut(s)	17, 299, 600, 1100
StuI	AGGCCT	1 cut(s)	997
StyD4I	CCNGG	3 cut(s)	107, 421, 565
TaiI	ACGT	2 cut(s)	701, 1071
TaqI	TCGA	3 cut(s)	543, 639, 858
TatI	WGTACW	2 cut(s)	1180, 1232
TfiI	GAWTC	5 cut(s)	114, 179, 418, 884, 1016
Tru1I	TTAA	5 cut(s)	72, 158, 1272, 1299, 1422
Tru9I	TTAA	5 cut(s)	72, 158, 1272, 1299, 1422
TscAI	CASTG	2 cut(s)	671, 1390
TseFI	GTSAC	2 cut(s)	319, 613
TseI	GCWGC	2 cut(s)	229, 1373
Tsp45I	GTSAC	2 cut(s)	319, 613
TspDTI	ATGAA	5 cut(s)	448, 514, 531, 732, 1343
TspGWI	ACGGA	2 cut(s)	172, 1187
TspRI	CASTG	2 cut(s)	671, 1390
Van91I	CCANNNNNTGG	1 cut(s)	1115
VpaK11BI	GGWCC	2 cut(s)	165, 767
XapI	RAATTY	3 cut(s)	944, 1037, 1144
XceI	RCATGY	1 cut(s)	297
XspI	CTAG	4 cut(s)	17, 299, 600, 1100
Zsp2I	ATGCAT	1 cut(s)	857

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.