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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AspS9I | GGNCC | 1 cut(s) | 148 |
| AsuHPI | GGTGA | 1 cut(s) | 50 |
| AvaII | GGWCC | 1 cut(s) | 148 |
| BccI | CCATC | 2 cut(s) | 17, 38 |
| Bme18I | GGWCC | 1 cut(s) | 148 |
| BmgT120I | GGNCC | 1 cut(s) | 148 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 157, 187 |
| BseGI | GGATG | 1 cut(s) | 141 |
| BseRI | GAGGAG | 1 cut(s) | 178 |
| Bst6I | CTCTTC | 2 cut(s) | 107, 189 |
| BstF5I | GGATG | 1 cut(s) | 141 |
| BtsCI | GGATG | 1 cut(s) | 141 |
| Cfr13I | GGNCC | 1 cut(s) | 148 |
| CviAII | CATG | 1 cut(s) | 152 |
| DraI | TTTAAA | 1 cut(s) | 177 |
| Eam1104I | CTCTTC | 2 cut(s) | 107, 189 |
| EarI | CTCTTC | 2 cut(s) | 107, 189 |
| Eco47I | GGWCC | 1 cut(s) | 148 |
| FaeI | CATG | 1 cut(s) | 155 |
| FaiI | YATR | 2 cut(s) | 11, 153 |
| FatI | CATG | 1 cut(s) | 151 |
| FokI | GGATG | 1 cut(s) | 148 |
| Hin1II | CATG | 1 cut(s) | 155 |
| HphI | GGTGA | 1 cut(s) | 50 |
| Hpy188I | TCNGA | 1 cut(s) | 125 |
| HpyAV | CCTTC | 3 cut(s) | 86, 97, 133 |
| Hsp92II | CATG | 1 cut(s) | 155 |
| MboII | GAAGA | 3 cut(s) | 32, 124, 197 |
| MluCI | AATT | 1 cut(s) | 143 |
| MnlI | CCTC | 2 cut(s) | 74, 156 |
| MseI | TTAA | 1 cut(s) | 176 |
| MslI | CAYNNNNRTG | 1 cut(s) | 156 |
| NlaIII | CATG | 1 cut(s) | 155 |
| PspPI | GGNCC | 1 cut(s) | 148 |
| RseI | CAYNNNNRTG | 1 cut(s) | 156 |
| SaqAI | TTAA | 1 cut(s) | 176 |
| Sau96I | GGNCC | 1 cut(s) | 148 |
| SetI | ASST | 2 cut(s) | 85, 97 |
| SgeI | CNNG | 1 cut(s) | 164 |
| SinI | GGWCC | 1 cut(s) | 148 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 156 |
| Sse9I | AATT | 1 cut(s) | 143 |
| TasI | AATT | 1 cut(s) | 143 |
| Tru1I | TTAA | 1 cut(s) | 176 |
| Tru9I | TTAA | 1 cut(s) | 176 |
| TspDTI | ATGAA | 4 cut(s) | 17, 105, 125, 152 |
| VpaK11BI | GGWCC | 1 cut(s) | 148 |