Rorug05G0251500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0001756
Physical Location & Seq
Reverse (-)
27555222 .. 27556755
1534 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0251500.1

Sequence Viewer

Length: 729 bp
ATGAAAGAGTGCGAGCTGTGTGGAGTGAGAGCTCGGATGTACTGCGAGGCGGACCGCGCGAGTTTATGCTGGGACTGCGACGAGAAAGTTCACGGAGCTAACTTTCTGGTGGCGAAGCATCCGAGAGTTCTTCTCTGTCACGTGTGCCAGTCGCAGACGCCGTGGACGGGTTGCGGAGCCAAGCTGACGCCGACTGTGTCGGTCTGCCAGAGTTGCGTTGGAAGCTGTAGTAAGATTGACCTGAACGAGGAGCAAGAAAGCGAGGCAGAGAATGAAGAAGAAGAAGAAGATTATTATGATGATGATGAAGATGAAGAAGATGATGACGTTGAGGATGAGGAGGAAGAGGATGAGAACCAGGTGGTTCCATGGTCATGCTGTTCTTACTCGCCGGCACAGCCTCCACCAGCAGTGAGTGGTTCGGATAGTGAAGCCGAGATTTCGGTGTCGAAGCGACTGCGAGAGAATGTAGATGTGGATTATTCTGATGATGAGGTAGGGTTTGATGGGATGTCCTCGTTTGGTGGTTCAATGAGACCGTTGAAAAGGCCGAAAGTGGGTGAAGAGAAGAATGGTGACGGAGATGATGGCGGTGAATCGGAGTGGAGAGCAACGGCGATCATGAGCTCAATTCAGAGGCTGGAAAAGGAAGTGATCGACGGCGCTGGTAATGCGTCCACCGCGGTTCTTAGGATTTGCAAACTCAGTAGAGATGAGAACAGCCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

242

Amino Acids

26.74

Weight (kDa)

4.21

Isoelectric Point (pI)

57.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-B_box PF00643 2 - 43 2.1e-06 B-box zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 57, 59, 683
AciI CCGC 6 cut(s) 50, 55, 174, 591, 681, 683
AcvI CACGTG 1 cut(s) 142
AcyI GRCGYC 2 cut(s) 158, 188
AfaI GTAC 1 cut(s) 41
AfiI CCNNNNNNNGG 3 cut(s) 167, 247, 557
AflIII ACRYGT 1 cut(s) 141
AgsI TTSAA 2 cut(s) 531, 544
AjnI CCWGG 1 cut(s) 357
AluBI AGCT 6 cut(s) 16, 32, 98, 184, 225, 627
AluI AGCT 6 cut(s) 16, 32, 98, 184, 225, 627
Alw21I GWGCWC 2 cut(s) 34, 629
Alw26I GTCTC 1 cut(s) 529
AlwNI CAGNNNCTG 1 cut(s) 640
AoxI GGCC 1 cut(s) 548
AspLEI GCGC 2 cut(s) 59, 665
AspS9I GGNCC 1 cut(s) 52
AsuHPI GGTGA 3 cut(s) 572, 587, 605
AvaII GGWCC 1 cut(s) 52
BanII GRGCYC 2 cut(s) 34, 629
BbrPI CACGTG 1 cut(s) 142
Bbv12I GWGCWC 2 cut(s) 34, 629
BccI CCATC 2 cut(s) 500, 581
BceAI ACGGC 3 cut(s) 145, 630, 676
BcgI CGANNNNNNTGC 2 cut(s) 439, 473
BciT130I CCWGG 1 cut(s) 359
BcoDI GTCTC 1 cut(s) 529
BfmI CTRYAG 1 cut(s) 226
BfoI RGCGCY 1 cut(s) 666
Bme1390I CCNGG 1 cut(s) 359
Bme18I GGWCC 1 cut(s) 52
BmgT120I GGNCC 1 cut(s) 52
BmiI GGNNCC 2 cut(s) 178, 366
BmrFI CCNGG 1 cut(s) 359
BmsI GCATC 1 cut(s) 127
BplI GAGNNNNNCTC 2 cut(s) 117, 149
BsaAI YACGTR 1 cut(s) 142
BsaHI GRCGYC 2 cut(s) 158, 188
BsaI GGTCTC 1 cut(s) 529
BsaJI CCNNGG 3 cut(s) 161, 368, 681
Bsc4I CCNNNNNNNGG 3 cut(s) 167, 247, 557
Bse118I RCCGGY 1 cut(s) 391
Bse1I ACTGG 1 cut(s) 148
BseBI CCWGG 1 cut(s) 359
BseDI CCNNGG 3 cut(s) 161, 368, 681
BseGI GGATG 5 cut(s) 42, 118, 340, 355, 516
BseLI CCNNNNNNNGG 3 cut(s) 167, 247, 557
BseMII CTCAG 1 cut(s) 718
BseNI ACTGG 1 cut(s) 148
BseRI GAGGAG 2 cut(s) 263, 353
BseYI CCCAGC 1 cut(s) 69
Bsh1236I CGCG 3 cut(s) 57, 59, 683
BshFI GGCC 1 cut(s) 550
BsiHKAI GWGCWC 2 cut(s) 34, 629
BsiSI CCGG 1 cut(s) 392
BslFI GGGAC 1 cut(s) 86
BslI CCNNNNNNNGG 3 cut(s) 167, 247, 557
BsmAI GTCTC 1 cut(s) 529
BsmFI GGGAC 1 cut(s) 86
BsnI GGCC 1 cut(s) 550
Bso31I GGTCTC 1 cut(s) 529
Bsp1286I GDGCHC 2 cut(s) 34, 629
Bsp143I GATC 2 cut(s) 618, 654
Bsp19I CCATGG 1 cut(s) 368
BspACI CCGC 6 cut(s) 50, 55, 174, 591, 681, 683
BspANI GGCC 1 cut(s) 550
BspCNI CTCAG 1 cut(s) 717
BspFNI CGCG 3 cut(s) 57, 59, 683
BspHI TCATGA 1 cut(s) 621
BspLI GGNNCC 2 cut(s) 178, 366
BspTNI GGTCTC 1 cut(s) 529
BsrFI RCCGGY 1 cut(s) 391
BsrI ACTGG 1 cut(s) 148
BssAI RCCGGY 1 cut(s) 391
BssECI CCNNGG 3 cut(s) 161, 368, 681
BssMI GATC 2 cut(s) 618, 654
BssNI GRCGYC 2 cut(s) 158, 188
BssT1I CCWWGG 1 cut(s) 368
Bst2UI CCWGG 1 cut(s) 359
Bst4CI ACNGT 2 cut(s) 196, 540
Bst6I CTCTTC 2 cut(s) 339, 558
BstACI GRCGYC 2 cut(s) 158, 188
BstBAI YACGTR 1 cut(s) 142
BstC8I GCNNGC 2 cut(s) 14, 393
BstDEI CTNAG 2 cut(s) 689, 704
BstDSI CCRYGG 3 cut(s) 161, 368, 681
BstENI CCTNNNNNAGG 1 cut(s) 245
BstF5I GGATG 5 cut(s) 42, 118, 340, 355, 516
BstFNI CGCG 3 cut(s) 57, 59, 683
BstH2I RGCGCY 1 cut(s) 666
BstHHI GCGC 2 cut(s) 59, 665
BstKTI GATC 2 cut(s) 621, 657
BstMAI GTCTC 1 cut(s) 529
BstMBI GATC 2 cut(s) 618, 654
BstMWI GCNNNNNNNGC 7 cut(s) 56, 75, 213, 222, 397, 671, 680
BstNI CCWGG 1 cut(s) 359
BstSCI CCNGG 1 cut(s) 357
BstSFI CTRYAG 1 cut(s) 226
BstUI CGCG 3 cut(s) 57, 59, 683
BsuRI GGCC 1 cut(s) 550
BtgI CCRYGG 3 cut(s) 161, 368, 681
BtsCI GGATG 5 cut(s) 42, 118, 340, 355, 516
BtsI GCAGTG 1 cut(s) 417
BtsIMutI CAGTG 1 cut(s) 417
Cac8I GCNNGC 2 cut(s) 14, 393
CaiI CAGNNNCTG 1 cut(s) 640
CciI TCATGA 1 cut(s) 621
CfoI GCGC 2 cut(s) 59, 665
Cfr10I RCCGGY 1 cut(s) 391
Cfr13I GGNCC 1 cut(s) 52
Cfr42I CCGCGG 1 cut(s) 684
CpoI CGGWCCG 1 cut(s) 52
CseI GACGC 3 cut(s) 166, 196, 663
CsiI ACCWGGT 1 cut(s) 357
Csp6I GTAC 1 cut(s) 40
CspI CGGWCCG 1 cut(s) 52
CviAII CATG 3 cut(s) 369, 375, 622
CviQI GTAC 1 cut(s) 40
DdeI CTNAG 2 cut(s) 689, 704
DpnI GATC 2 cut(s) 620, 656
DpnII GATC 2 cut(s) 618, 654
Eam1104I CTCTTC 2 cut(s) 339, 558
EarI CTCTTC 2 cut(s) 339, 558
EciI GGCGGA 1 cut(s) 65
Ecl136II GAGCTC 2 cut(s) 32, 627
Eco130I CCWWGG 1 cut(s) 368
Eco24I GRGCYC 2 cut(s) 34, 629
Eco31I GGTCTC 1 cut(s) 529
Eco47I GGWCC 1 cut(s) 52
Eco53kI GAGCTC 2 cut(s) 32, 627
Eco72I CACGTG 1 cut(s) 142
EcoICRI GAGCTC 2 cut(s) 32, 627
EcoNI CCTNNNNNAGG 1 cut(s) 245
EcoRII CCWGG 1 cut(s) 357
EcoT14I CCWWGG 1 cut(s) 368
EcoT38I GRGCYC 2 cut(s) 34, 629
ErhI CCWWGG 1 cut(s) 368
FaeI CATG 3 cut(s) 372, 378, 625
FaiI YATR 5 cut(s) 67, 297, 370, 376, 623
FaqI GGGAC 1 cut(s) 86
FatI CATG 3 cut(s) 368, 374, 621
FokI GGATG 5 cut(s) 49, 105, 347, 362, 523
FriOI GRGCYC 2 cut(s) 34, 629
GlaI GCGC 2 cut(s) 58, 664
GsaI CCCAGC 1 cut(s) 73
HaeII RGCGCY 1 cut(s) 666
HaeIII GGCC 1 cut(s) 550
HapII CCGG 1 cut(s) 392
HgaI GACGC 3 cut(s) 166, 196, 663
HhaI GCGC 2 cut(s) 59, 665
Hin1I GRCGYC 2 cut(s) 158, 188
Hin1II CATG 3 cut(s) 372, 378, 625
Hin6I GCGC 2 cut(s) 57, 663
HinP1I GCGC 2 cut(s) 57, 663
HinfI GANTC 1 cut(s) 596
HpaII CCGG 1 cut(s) 392
HphI GGTGA 3 cut(s) 572, 587, 605
Hpy166II GTNNAC 3 cut(s) 91, 165, 678
Hpy188I TCNGA 6 cut(s) 36, 123, 424, 487, 601, 636
Hpy188III TCNNGA 1 cut(s) 622
Hpy8I GTNNAC 3 cut(s) 91, 165, 678
Hpy99I CGWCG 2 cut(s) 83, 662
HpyCH4III ACNGT 2 cut(s) 196, 540
HpyCH4IV ACGT 2 cut(s) 141, 327
HpyCH4V TGCA 1 cut(s) 699
HpyF10VI GCNNNNNNNGC 7 cut(s) 56, 75, 213, 222, 397, 671, 680
HpyF3I CTNAG 2 cut(s) 689, 704
HpySE526I ACGT 2 cut(s) 141, 327
Hsp92I GRCGYC 2 cut(s) 158, 188
Hsp92II CATG 3 cut(s) 372, 378, 625
HspAI GCGC 2 cut(s) 57, 663
KroI GCCGGC 1 cut(s) 391
KroNI GCCGGC 1 cut(s) 393
KspI CCGCGG 1 cut(s) 684
Kzo9I GATC 2 cut(s) 618, 654
LmnI GCTCC 3 cut(s) 95, 176, 250
LweI GCATC 1 cut(s) 127
MabI ACCWGGT 1 cut(s) 357
MaeII ACGT 2 cut(s) 141, 327
MaeIII GTNAC 2 cut(s) 137, 575
MalI GATC 2 cut(s) 620, 656
MboI GATC 2 cut(s) 618, 654
MhlI GDGCHC 2 cut(s) 34, 629
MluCI AATT 1 cut(s) 630
MmeI TCCRAC 1 cut(s) 199
MroNI GCCGGC 1 cut(s) 391
MslI CAYNNNNRTG 1 cut(s) 373
MspA1I CMGCKG 1 cut(s) 683
MspI CCGG 1 cut(s) 392
MspR9I CCNGG 1 cut(s) 359
MvaI CCWGG 1 cut(s) 359
MvnI CGCG 3 cut(s) 57, 59, 683
MwoI GCNNNNNNNGC 7 cut(s) 56, 75, 213, 222, 397, 671, 680
NaeI GCCGGC 1 cut(s) 393
NcoI CCATGG 1 cut(s) 368
NdeII GATC 2 cut(s) 618, 654
NgoMIV GCCGGC 1 cut(s) 391
NlaIII CATG 3 cut(s) 372, 378, 625
NlaIV GGNNCC 2 cut(s) 178, 366
NmeAIII GCCGAG 1 cut(s) 460
NmuCI GTSAC 2 cut(s) 137, 575
PagI TCATGA 1 cut(s) 621
PcsI WCGNNNNNNNCGW 1 cut(s) 158
PdiI GCCGGC 1 cut(s) 393
PfeI GAWTC 1 cut(s) 596
PflFI GACNNNGTC 1 cut(s) 196
PmaCI CACGTG 1 cut(s) 142
PmlI CACGTG 1 cut(s) 142
Ppu21I YACGTR 1 cut(s) 142
Psp124BI GAGCTC 2 cut(s) 34, 629
Psp6I CCWGG 1 cut(s) 357
PspCI CACGTG 1 cut(s) 142
PspFI CCCAGC 1 cut(s) 69
PspGI CCWGG 1 cut(s) 357
PspN4I GGNNCC 2 cut(s) 178, 366
PspPI GGNCC 1 cut(s) 52
PstNI CAGNNNCTG 1 cut(s) 640
PsyI GACNNNGTC 1 cut(s) 196
RsaI GTAC 1 cut(s) 41
RsaNI GTAC 1 cut(s) 40
RseI CAYNNNNRTG 1 cut(s) 373
Rsr2I CGGWCCG 1 cut(s) 52
RsrII CGGWCCG 1 cut(s) 52
SacI GAGCTC 2 cut(s) 34, 629
SacII CCGCGG 1 cut(s) 684
Sau3AI GATC 2 cut(s) 618, 654
Sau96I GGNCC 1 cut(s) 52
ScrFI CCNGG 1 cut(s) 359
SduI GDGCHC 2 cut(s) 34, 629
SexAI ACCWGGT 1 cut(s) 357
SfaNI GCATC 1 cut(s) 127
SfcI CTRYAG 1 cut(s) 226
Sfr303I CCGCGG 1 cut(s) 684
SgrBI CCGCGG 1 cut(s) 684
SinI GGWCC 1 cut(s) 52
SmiMI CAYNNNNRTG 1 cut(s) 373
Sse9I AATT 1 cut(s) 630
SsiI CCGC 6 cut(s) 50, 55, 174, 591, 681, 683
SstI GAGCTC 2 cut(s) 34, 629
StyD4I CCNGG 1 cut(s) 357
StyI CCWWGG 1 cut(s) 368
TaaI ACNGT 2 cut(s) 196, 540
TaiI ACGT 2 cut(s) 144, 330
TaqI TCGA 2 cut(s) 449, 657
TaqII GACCGA 1 cut(s) 190
TasI AATT 1 cut(s) 630
TatI WGTACW 1 cut(s) 39
TfiI GAWTC 1 cut(s) 596
TscAI CASTG 1 cut(s) 417
TseFI GTSAC 2 cut(s) 137, 575
Tsp45I GTSAC 2 cut(s) 137, 575
TspDTI ATGAA 4 cut(s) 17, 288, 321, 327
TspGWI ACGGA 2 cut(s) 108, 594
TspRI CASTG 1 cut(s) 417
Tth111I GACNNNGTC 1 cut(s) 196
VpaK11BI GGWCC 1 cut(s) 52
XagI CCTNNNNNAGG 1 cut(s) 245
XcmI CCANNNNNNNNNTGG 1 cut(s) 215
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.