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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcoI | YGGCCR | 1 cut(s) | 98 |
| AgsI | TTSAA | 1 cut(s) | 11 |
| AluBI | AGCT | 1 cut(s) | 46 |
| AluI | AGCT | 1 cut(s) | 46 |
| AoxI | GGCC | 1 cut(s) | 98 |
| AsuHPI | GGTGA | 3 cut(s) | 68, 86, 148 |
| BalI | TGGCCA | 1 cut(s) | 100 |
| BccI | CCATC | 5 cut(s) | 16, 28, 47, 113, 133 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 114, 144 |
| BseGI | GGATG | 3 cut(s) | 39, 43, 187 |
| BseRI | GAGGAG | 1 cut(s) | 164 |
| BshFI | GGCC | 1 cut(s) | 100 |
| BsnI | GGCC | 1 cut(s) | 100 |
| BspANI | GGCC | 1 cut(s) | 100 |
| Bst4CI | ACNGT | 1 cut(s) | 74 |
| BstF5I | GGATG | 3 cut(s) | 39, 43, 187 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 97 |
| BsuRI | GGCC | 1 cut(s) | 100 |
| BtsCI | GGATG | 3 cut(s) | 39, 43, 187 |
| CviJI | RGCY | 2 cut(s) | 46, 100 |
| CviKI_1 | RGCY | 2 cut(s) | 46, 100 |
| EaeI | YGGCCR | 1 cut(s) | 98 |
| FaiI | YATR | 5 cut(s) | 89, 156, 165, 191, 202 |
| FokI | GGATG | 3 cut(s) | 46, 50, 194 |
| HaeIII | GGCC | 1 cut(s) | 100 |
| HindIII | AAGCTT | 1 cut(s) | 44 |
| HinfI | GANTC | 1 cut(s) | 11 |
| HphI | GGTGA | 3 cut(s) | 68, 86, 148 |
| Hpy188III | TCNNGA | 1 cut(s) | 50 |
| HpyCH4III | ACNGT | 1 cut(s) | 74 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 97 |
| LmnI | GCTCC | 2 cut(s) | 9, 96 |
| MboII | GAAGA | 1 cut(s) | 92 |
| MlsI | TGGCCA | 1 cut(s) | 100 |
| MluNI | TGGCCA | 1 cut(s) | 100 |
| MnlI | CCTC | 4 cut(s) | 25, 136, 142, 187 |
| Mox20I | TGGCCA | 1 cut(s) | 100 |
| MscI | TGGCCA | 1 cut(s) | 100 |
| Msp20I | TGGCCA | 1 cut(s) | 100 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 97 |
| PfeI | GAWTC | 1 cut(s) | 11 |
| SetI | ASST | 3 cut(s) | 48, 147, 198 |
| SgeI | CNNG | 3 cut(s) | 20, 31, 62 |
| TaaI | ACNGT | 1 cut(s) | 74 |
| TfiI | GAWTC | 1 cut(s) | 11 |
| TspDTI | ATGAA | 2 cut(s) | 78, 93 |