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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcoI | YGGCCR | 1 cut(s) | 98 |
| AgsI | TTSAA | 1 cut(s) | 11 |
| AluBI | AGCT | 1 cut(s) | 46 |
| AluI | AGCT | 1 cut(s) | 46 |
| AoxI | GGCC | 1 cut(s) | 98 |
| AsuHPI | GGTGA | 2 cut(s) | 86, 148 |
| BalI | TGGCCA | 1 cut(s) | 100 |
| BccI | CCATC | 6 cut(s) | 16, 28, 47, 113, 133, 184 |
| BfaI | CTAG | 1 cut(s) | 50 |
| BmsI | GCATC | 1 cut(s) | 78 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 114, 144 |
| BseGI | GGATG | 2 cut(s) | 39, 43 |
| BshFI | GGCC | 1 cut(s) | 100 |
| BsnI | GGCC | 1 cut(s) | 100 |
| Bsp143I | GATC | 1 cut(s) | 3 |
| BspANI | GGCC | 1 cut(s) | 100 |
| BssMI | GATC | 1 cut(s) | 3 |
| BstF5I | GGATG | 2 cut(s) | 39, 43 |
| BstKTI | GATC | 1 cut(s) | 6 |
| BstMBI | GATC | 1 cut(s) | 3 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 97 |
| BsuRI | GGCC | 1 cut(s) | 100 |
| BtsCI | GGATG | 2 cut(s) | 39, 43 |
| CviJI | RGCY | 2 cut(s) | 46, 100 |
| CviKI_1 | RGCY | 2 cut(s) | 46, 100 |
| DpnI | GATC | 1 cut(s) | 5 |
| DpnII | GATC | 1 cut(s) | 3 |
| EaeI | YGGCCR | 1 cut(s) | 98 |
| FaiI | YATR | 4 cut(s) | 86, 156, 165, 202 |
| FokI | GGATG | 2 cut(s) | 46, 50 |
| FspBI | CTAG | 1 cut(s) | 50 |
| HaeIII | GGCC | 1 cut(s) | 100 |
| HindIII | AAGCTT | 1 cut(s) | 44 |
| HinfI | GANTC | 1 cut(s) | 11 |
| HphI | GGTGA | 2 cut(s) | 86, 148 |
| Hpy188III | TCNNGA | 2 cut(s) | 19, 50 |
| HpyAV | CCTTC | 1 cut(s) | 25 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 97 |
| Kzo9I | GATC | 1 cut(s) | 3 |
| LweI | GCATC | 1 cut(s) | 78 |
| MaeI | CTAG | 1 cut(s) | 50 |
| MalI | GATC | 1 cut(s) | 5 |
| MboI | GATC | 1 cut(s) | 3 |
| MboII | GAAGA | 1 cut(s) | 92 |
| MlsI | TGGCCA | 1 cut(s) | 100 |
| MluNI | TGGCCA | 1 cut(s) | 100 |
| MnlI | CCTC | 4 cut(s) | 136, 142, 175, 187 |
| Mox20I | TGGCCA | 1 cut(s) | 100 |
| MscI | TGGCCA | 1 cut(s) | 100 |
| Msp20I | TGGCCA | 1 cut(s) | 100 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 97 |
| NdeII | GATC | 1 cut(s) | 3 |
| PfeI | GAWTC | 1 cut(s) | 11 |
| Sau3AI | GATC | 1 cut(s) | 3 |
| SetI | ASST | 4 cut(s) | 48, 147, 186, 198 |
| SfaNI | GCATC | 1 cut(s) | 78 |
| SgeI | CNNG | 3 cut(s) | 20, 31, 62 |
| SspMI | CTAG | 1 cut(s) | 50 |
| TfiI | GAWTC | 1 cut(s) | 11 |
| TspDTI | ATGAA | 2 cut(s) | 78, 93 |
| XbaI | TCTAGA | 1 cut(s) | 49 |
| XspI | CTAG | 1 cut(s) | 50 |