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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcsI | RAATTY | 1 cut(s) | 5 |
| ApoI | RAATTY | 1 cut(s) | 5 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 147 |
| AsuHPI | GGTGA | 1 cut(s) | 50 |
| BccI | CCATC | 3 cut(s) | 17, 38, 83 |
| BpuEI | CTTGAG | 1 cut(s) | 173 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 84, 114, 157, 187 |
| BseGI | GGATG | 1 cut(s) | 141 |
| BseRI | GAGGAG | 1 cut(s) | 178 |
| Bst6I | CTCTTC | 2 cut(s) | 107, 189 |
| BstF5I | GGATG | 1 cut(s) | 141 |
| BtsCI | GGATG | 1 cut(s) | 141 |
| DraI | TTTAAA | 1 cut(s) | 177 |
| Eam1104I | CTCTTC | 2 cut(s) | 107, 189 |
| EarI | CTCTTC | 2 cut(s) | 107, 189 |
| FaiI | YATR | 1 cut(s) | 11 |
| FokI | GGATG | 1 cut(s) | 148 |
| HphI | GGTGA | 1 cut(s) | 50 |
| Hpy188I | TCNGA | 1 cut(s) | 125 |
| Hpy188III | TCNNGA | 1 cut(s) | 152 |
| HpyAV | CCTTC | 2 cut(s) | 97, 133 |
| MboII | GAAGA | 3 cut(s) | 32, 124, 197 |
| MluCI | AATT | 2 cut(s) | 5, 143 |
| MnlI | CCTC | 3 cut(s) | 74, 86, 156 |
| MroXI | GAANNNNTTC | 1 cut(s) | 147 |
| MseI | TTAA | 1 cut(s) | 176 |
| PdmI | GAANNNNTTC | 1 cut(s) | 147 |
| SaqAI | TTAA | 1 cut(s) | 176 |
| SetI | ASST | 1 cut(s) | 85 |
| SgeI | CNNG | 1 cut(s) | 164 |
| SmlI | CTYRAG | 1 cut(s) | 152 |
| SmoI | CTYRAG | 1 cut(s) | 152 |
| Sse9I | AATT | 2 cut(s) | 5, 143 |
| TasI | AATT | 2 cut(s) | 5, 143 |
| Tru1I | TTAA | 1 cut(s) | 176 |
| Tru9I | TTAA | 1 cut(s) | 176 |
| TspDTI | ATGAA | 3 cut(s) | 17, 125, 152 |
| XapI | RAATTY | 1 cut(s) | 5 |
| XmnI | GAANNNNTTC | 1 cut(s) | 147 |