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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccBSI | CCGCTC | 1 cut(s) | 103 |
| AciI | CCGC | 1 cut(s) | 103 |
| AcsI | RAATTY | 1 cut(s) | 263 |
| ApoI | RAATTY | 1 cut(s) | 263 |
| AspS9I | GGNCC | 1 cut(s) | 105 |
| AsuHPI | GGTGA | 1 cut(s) | 170 |
| AvaII | GGWCC | 1 cut(s) | 105 |
| BccI | CCATC | 4 cut(s) | 137, 158, 203, 253 |
| BceAI | ACGGC | 1 cut(s) | 109 |
| BcgI | CGANNNNNNTGC | 1 cut(s) | 36 |
| Bme18I | GGWCC | 1 cut(s) | 105 |
| BmgT120I | GGNCC | 1 cut(s) | 105 |
| BmsI | GCATC | 3 cut(s) | 10, 70, 107 |
| BpuEI | CTTGAG | 1 cut(s) | 293 |
| BsaJI | CCNNGG | 1 cut(s) | 108 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 204, 234, 277, 307 |
| Bse1I | ACTGG | 4 cut(s) | 32, 49, 70, 92 |
| BseDI | CCNNGG | 1 cut(s) | 108 |
| BseGI | GGATG | 1 cut(s) | 261 |
| BseMII | CTCAG | 1 cut(s) | 41 |
| BseNI | ACTGG | 4 cut(s) | 32, 49, 70, 92 |
| BseRI | GAGGAG | 1 cut(s) | 298 |
| BspACI | CCGC | 1 cut(s) | 103 |
| BspCNI | CTCAG | 1 cut(s) | 42 |
| BsrBI | CCGCTC | 1 cut(s) | 103 |
| BsrI | ACTGG | 4 cut(s) | 32, 49, 70, 92 |
| BssECI | CCNNGG | 1 cut(s) | 108 |
| Bst6I | CTCTTC | 2 cut(s) | 227, 309 |
| BstC8I | GCNNGC | 1 cut(s) | 122 |
| BstDEI | CTNAG | 1 cut(s) | 50 |
| BstF5I | GGATG | 1 cut(s) | 261 |
| BtsCI | GGATG | 1 cut(s) | 261 |
| Cac8I | GCNNGC | 1 cut(s) | 122 |
| Cfr13I | GGNCC | 1 cut(s) | 105 |
| CpoI | CGGWCCG | 1 cut(s) | 105 |
| CspI | CGGWCCG | 1 cut(s) | 105 |
| CviJI | RGCY | 1 cut(s) | 124 |
| CviKI_1 | RGCY | 1 cut(s) | 124 |
| DdeI | CTNAG | 1 cut(s) | 50 |
| DraI | TTTAAA | 1 cut(s) | 297 |
| Eam1104I | CTCTTC | 2 cut(s) | 227, 309 |
| EarI | CTCTTC | 2 cut(s) | 227, 309 |
| Eco47I | GGWCC | 1 cut(s) | 105 |
| FaiI | YATR | 2 cut(s) | 59, 131 |
| FokI | GGATG | 1 cut(s) | 268 |
| HphI | GGTGA | 1 cut(s) | 170 |
| Hpy188I | TCNGA | 1 cut(s) | 245 |
| HpyAV | CCTTC | 1 cut(s) | 217 |
| HpyF3I | CTNAG | 1 cut(s) | 50 |
| LpnPI | CCDG | 4 cut(s) | 13, 30, 51, 73 |
| LweI | GCATC | 3 cut(s) | 10, 70, 107 |
| MbiI | CCGCTC | 1 cut(s) | 103 |
| MboII | GAAGA | 3 cut(s) | 152, 244, 317 |
| MluCI | AATT | 1 cut(s) | 263 |
| MnlI | CCTC | 7 cut(s) | 7, 45, 66, 103, 194, 206, 276 |
| MseI | TTAA | 1 cut(s) | 296 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 105 |
| PspPI | GGNCC | 1 cut(s) | 105 |
| Rsr2I | CGGWCCG | 1 cut(s) | 105 |
| RsrII | CGGWCCG | 1 cut(s) | 105 |
| SaqAI | TTAA | 1 cut(s) | 296 |
| Sau96I | GGNCC | 1 cut(s) | 105 |
| SetI | ASST | 3 cut(s) | 18, 205, 217 |
| SfaNI | GCATC | 3 cut(s) | 10, 70, 107 |
| SgeI | CNNG | 9 cut(s) | 25, 40, 57, 78, 100, 112, 121, 133, 284 |
| SinI | GGWCC | 1 cut(s) | 105 |
| SmlI | CTYRAG | 1 cut(s) | 272 |
| SmoI | CTYRAG | 1 cut(s) | 272 |
| Sse9I | AATT | 1 cut(s) | 263 |
| SsiI | CCGC | 1 cut(s) | 103 |
| TaqI | TCGA | 1 cut(s) | 18 |
| TaqII | GACCGA | 1 cut(s) | 122 |
| TasI | AATT | 1 cut(s) | 263 |
| Tru1I | TTAA | 1 cut(s) | 296 |
| Tru9I | TTAA | 1 cut(s) | 296 |
| TspDTI | ATGAA | 2 cut(s) | 17, 245 |
| TspGWI | ACGGA | 1 cut(s) | 22 |
| VpaK11BI | GGWCC | 1 cut(s) | 105 |
| XapI | RAATTY | 1 cut(s) | 263 |