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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AgsI | TTSAA | 1 cut(s) | 149 |
| ApeKI | GCWGC | 1 cut(s) | 40 |
| BbvI | GCAGC | 1 cut(s) | 52 |
| BisI | GCNGC | 1 cut(s) | 41 |
| BlsI | GCNGC | 1 cut(s) | 42 |
| Bse1I | ACTGG | 1 cut(s) | 108 |
| BseNI | ACTGG | 1 cut(s) | 108 |
| BseXI | GCAGC | 1 cut(s) | 52 |
| BspHI | TCATGA | 1 cut(s) | 15 |
| BsrI | ACTGG | 1 cut(s) | 108 |
| Bst6I | CTCTTC | 1 cut(s) | 150 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 84 |
| BstV1I | GCAGC | 1 cut(s) | 52 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 115 |
| BtsIMutI | CAGTG | 1 cut(s) | 115 |
| CciI | TCATGA | 1 cut(s) | 15 |
| CviAII | CATG | 1 cut(s) | 16 |
| Eam1104I | CTCTTC | 1 cut(s) | 150 |
| EarI | CTCTTC | 1 cut(s) | 150 |
| FaeI | CATG | 1 cut(s) | 19 |
| FaiI | YATR | 3 cut(s) | 17, 116, 168 |
| FatI | CATG | 1 cut(s) | 15 |
| Fnu4HI | GCNGC | 1 cut(s) | 41 |
| Fsp4HI | GCNGC | 1 cut(s) | 41 |
| FspEI | CC | 7 cut(s) | 9, 71, 85, 101, 106, 121, 156 |
| GluI | GCNGC | 1 cut(s) | 41 |
| Hin1II | CATG | 1 cut(s) | 19 |
| Hpy188III | TCNNGA | 1 cut(s) | 16 |
| Hpy99I | CGWCG | 1 cut(s) | 164 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 84 |
| Hsp92II | CATG | 1 cut(s) | 19 |
| LpnPI | CCDG | 2 cut(s) | 106, 121 |
| Lsp1109I | GCAGC | 1 cut(s) | 52 |
| MboII | GAAGA | 2 cut(s) | 31, 137 |
| MluCI | AATT | 3 cut(s) | 11, 28, 149 |
| MnlI | CCTC | 1 cut(s) | 153 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 84 |
| NlaIII | CATG | 1 cut(s) | 19 |
| PagI | TCATGA | 1 cut(s) | 15 |
| PkrI | GCNGC | 1 cut(s) | 42 |
| SatI | GCNGC | 1 cut(s) | 41 |
| SgeI | CNNG | 4 cut(s) | 28, 91, 120, 133 |
| Sse9I | AATT | 3 cut(s) | 11, 28, 149 |
| TasI | AATT | 3 cut(s) | 11, 28, 149 |
| TscAI | CASTG | 1 cut(s) | 115 |
| TseI | GCWGC | 1 cut(s) | 40 |
| TspDTI | ATGAA | 6 cut(s) | 4, 32, 62, 81, 110, 145 |
| TspRI | CASTG | 1 cut(s) | 115 |