Rroxscaffold_4G00306180

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0001756
Physical Location & Seq
Reverse (-)
26974347 .. 26975357
1011 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00306180.1

Sequence Viewer

Length: 654 bp
ATGCGACCCGTAACGGGAGGCACTAGCCTCGCTTCGACCCCGTCTACGGAAAGCGGTCACCGGATGATCCAGTGCGGTAGACTTGTTCTTGGAAAGCTCGAGGCATTTGCCACCACCACCATTACCACGGCCCTCAAAGAGCTTACGGACAAGTTCGTAGCTCGATTCATCTTACGTACCGATGGGATACATACCCCAACGGCCTTTGCTAAAGATTCAACGGGGGAGAATGAAACTTTGAAGTCTTTCGTTAACCGATGGCGGGCGGATACCGCTAAGTGCGGGACCTTAACAAGGAAGCTGGCCGAGCTGGCTTTCGGGGAGGGCCTAAGGCGCGGAGAGTTTCTCTACCGGATTAACCATCACCCCCCGGCTAGCTACGACGAGGCGATGGCTACGGCCATCGGGCACGCTCAAGTCGGGTCGAAACGTATGGAGATAACGTCGAGACGAGCTAAGGGTCTCCACCCGGCCCCCGTGGTTAGGGATGAGCCACGAAAAGGGAGTGGGCCCATAATCAAGATGGATCACCCCACAACTCAAACAAGAGAAGCAAAGAGTCAACATCCGGGGTCTAGCGGTTACGTGACCCCTCGGCCTCGCCGGGAAGGAAGGCCACGGCGAACCCGCGAACGGCACCACCACCCCGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

217

Amino Acids

24.05

Weight (kDa)

10.61

Isoelectric Point (pI)

35.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 636
AccI GTMKAC 2 cut(s) 44, 79
AccII CGCG 2 cut(s) 336, 630
AciI CCGC 9 cut(s) 54, 75, 262, 266, 273, 282, 336, 579, 628
AclWI GGATC 2 cut(s) 61, 534
AcoI YGGCCR 2 cut(s) 303, 399
AfaI GTAC 1 cut(s) 178
AfiI CCNNNNNNNGG 7 cut(s) 14, 46, 262, 294, 483, 500, 633
AgsI TTSAA 2 cut(s) 219, 241
AluBI AGCT 7 cut(s) 97, 142, 161, 301, 310, 378, 455
AluI AGCT 7 cut(s) 97, 142, 161, 301, 310, 378, 455
Alw26I GTCTC 2 cut(s) 442, 467
AlwI GGATC 2 cut(s) 61, 534
Ama87I CYCGRG 1 cut(s) 98
AoxI GGCC 9 cut(s) 129, 201, 303, 325, 399, 471, 509, 596, 614
ApaI GGGCCC 1 cut(s) 513
Asp700I GAANNNNTTC 1 cut(s) 245
AspLEI GCGC 1 cut(s) 336
AspS9I GGNCC 6 cut(s) 130, 285, 325, 472, 509, 510
AsuC2I CCSGG 4 cut(s) 371, 470, 570, 605
AsuHPI GGTGA 3 cut(s) 50, 356, 521
AsuNHI GCTAGC 1 cut(s) 374
AvaI CYCGRG 1 cut(s) 98
AvaII GGWCC 1 cut(s) 285
AxyI CCTNAGG 1 cut(s) 329
BaeGI GKGCMC 2 cut(s) 411, 513
BanI GGYRCC 1 cut(s) 636
BanII GRGCYC 1 cut(s) 513
BccI CCATC 6 cut(s) 176, 252, 369, 385, 410, 517
BceAI ACGGC 5 cut(s) 144, 216, 414, 635, 650
BcgI CGANNNNNNTGC 4 cut(s) 10, 44, 89, 123
BciVI GTATCC 2 cut(s) 180, 262
BcnI CCSGG 4 cut(s) 371, 470, 570, 605
BcoDI GTCTC 2 cut(s) 442, 467
BfaI CTAG 3 cut(s) 24, 375, 576
BfuI GTATCC 2 cut(s) 180, 262
BglI GCCNNNNNGGC 1 cut(s) 311
Bme1390I CCNGG 4 cut(s) 371, 470, 570, 605
Bme18I GGWCC 1 cut(s) 285
BmeT110I CYCGRG 1 cut(s) 98
BmgT120I GGNCC 6 cut(s) 130, 285, 325, 472, 509, 510
BmiI GGNNCC 4 cut(s) 286, 474, 511, 638
BmrFI CCNGG 4 cut(s) 371, 470, 570, 605
BmtI GCTAGC 1 cut(s) 378
BplI GAGNNNNNCTC 2 cut(s) 330, 362
Bpu10I CCTNAGC 1 cut(s) 456
BpuEI CTTGAG 1 cut(s) 399
BpuMI CCSGG 4 cut(s) 371, 470, 570, 605
BsaAI YACGTR 2 cut(s) 176, 586
BsaI GGTCTC 1 cut(s) 467
BsaJI CCNNGG 6 cut(s) 126, 369, 477, 569, 593, 617
BsaWI WCCGGW 2 cut(s) 60, 351
BsaXI ACNNNNNCTCC 2 cut(s) 428, 458
Bsc4I CCNNNNNNNGG 7 cut(s) 14, 46, 262, 294, 483, 500, 633
Bse1I ACTGG 1 cut(s) 70
Bse21I CCTNAGG 1 cut(s) 329
BseDI CCNNGG 6 cut(s) 126, 369, 477, 569, 593, 617
BseGI GGATG 3 cut(s) 69, 493, 565
BseLI CCNNNNNNNGG 7 cut(s) 14, 46, 262, 294, 483, 500, 633
BseNI ACTGG 1 cut(s) 70
BseSI GKGCMC 2 cut(s) 411, 513
Bsh1236I CGCG 2 cut(s) 336, 630
BshFI GGCC 9 cut(s) 131, 203, 305, 327, 401, 473, 511, 598, 616
BshNI GGYRCC 1 cut(s) 636
BsiHKCI CYCGRG 1 cut(s) 98
BsiSI CCGG 6 cut(s) 61, 352, 371, 470, 569, 604
BslFI GGGAC 1 cut(s) 298
BslI CCNNNNNNNGG 7 cut(s) 14, 46, 262, 294, 483, 500, 633
BsmAI GTCTC 2 cut(s) 442, 467
BsmBI CGTCTC 1 cut(s) 442
BsmFI GGGAC 1 cut(s) 298
BsnI GGCC 9 cut(s) 131, 203, 305, 327, 401, 473, 511, 598, 616
Bso31I GGTCTC 1 cut(s) 467
BsoBI CYCGRG 1 cut(s) 98
Bsp120I GGGCCC 1 cut(s) 509
Bsp1286I GDGCHC 2 cut(s) 411, 513
Bsp143I GATC 2 cut(s) 66, 526
BspACI CCGC 9 cut(s) 54, 75, 262, 266, 273, 282, 336, 579, 628
BspANI GGCC 9 cut(s) 131, 203, 305, 327, 401, 473, 511, 598, 616
BspFNI CGCG 2 cut(s) 336, 630
BspLI GGNNCC 4 cut(s) 286, 474, 511, 638
BspOI GCTAGC 1 cut(s) 378
BspPI GGATC 2 cut(s) 61, 534
BspT107I GGYRCC 1 cut(s) 636
BspTNI GGTCTC 1 cut(s) 467
BsrI ACTGG 1 cut(s) 70
BssECI CCNNGG 6 cut(s) 126, 369, 477, 569, 593, 617
BssMI GATC 2 cut(s) 66, 526
BstBAI YACGTR 2 cut(s) 176, 586
BstC8I GCNNGC 5 cut(s) 264, 303, 312, 376, 411
BstDEI CTNAG 3 cut(s) 276, 329, 456
BstDSI CCRYGG 3 cut(s) 126, 477, 617
BstEII GGTNACC 1 cut(s) 56
BstENI CCTNNNNNAGG 1 cut(s) 292
BstF5I GGATG 3 cut(s) 69, 493, 565
BstFNI CGCG 2 cut(s) 336, 630
BstHHI GCGC 1 cut(s) 336
BstKTI GATC 2 cut(s) 69, 529
BstMAI GTCTC 2 cut(s) 442, 467
BstMBI GATC 2 cut(s) 66, 526
BstMWI GCNNNNNNNGC 4 cut(s) 272, 307, 311, 333
BstPI GGTNACC 1 cut(s) 56
BstSCI CCNGG 4 cut(s) 369, 468, 568, 603
BstSLI GKGCMC 2 cut(s) 411, 513
BstSNI TACGTA 1 cut(s) 176
BstUI CGCG 2 cut(s) 336, 630
Bsu36I CCTNAGG 1 cut(s) 329
BsuI GTATCC 2 cut(s) 180, 262
BsuRI GGCC 9 cut(s) 131, 203, 305, 327, 401, 473, 511, 598, 616
BtgI CCRYGG 3 cut(s) 126, 477, 617
BtgZI GCGATG 1 cut(s) 404
BtsCI GGATG 3 cut(s) 69, 493, 565
BtsIMutI CAGTG 1 cut(s) 77
Cac8I GCNNGC 5 cut(s) 264, 303, 312, 376, 411
CfoI GCGC 1 cut(s) 336
Cfr13I GGNCC 6 cut(s) 130, 285, 325, 472, 509, 510
Csp6I GTAC 1 cut(s) 177
CviQI GTAC 1 cut(s) 177
DdeI CTNAG 3 cut(s) 276, 329, 456
DpnI GATC 2 cut(s) 68, 528
DpnII GATC 2 cut(s) 66, 526
EaeI YGGCCR 2 cut(s) 303, 399
EciI GGCGGA 1 cut(s) 281
Eco105I TACGTA 1 cut(s) 176
Eco24I GRGCYC 1 cut(s) 513
Eco31I GGTCTC 1 cut(s) 467
Eco47I GGWCC 1 cut(s) 285
Eco81I CCTNAGG 1 cut(s) 329
Eco88I CYCGRG 1 cut(s) 98
Eco91I GGTNACC 1 cut(s) 56
EcoNI CCTNNNNNAGG 1 cut(s) 292
EcoO109I RGGNCCY 2 cut(s) 285, 325
EcoO65I GGTNACC 1 cut(s) 56
EcoT38I GRGCYC 1 cut(s) 513
Esp3I CGTCTC 1 cut(s) 442
FaiI YATR 4 cut(s) 192, 434, 515, 652
FaqI GGGAC 1 cut(s) 298
FauI CCCGC 3 cut(s) 255, 275, 635
FblI GTMKAC 2 cut(s) 44, 79
FokI GGATG 3 cut(s) 76, 500, 552
FriOI GRGCYC 1 cut(s) 513
FspBI CTAG 3 cut(s) 24, 375, 576
GlaI GCGC 1 cut(s) 335
HaeIII GGCC 9 cut(s) 131, 203, 305, 327, 401, 473, 511, 598, 616
HapII CCGG 6 cut(s) 61, 352, 371, 470, 569, 604
HhaI GCGC 1 cut(s) 336
Hin6I GCGC 1 cut(s) 334
HinP1I GCGC 1 cut(s) 334
HincII GTYRAC 2 cut(s) 253, 563
HindII GTYRAC 2 cut(s) 253, 563
HinfI GANTC 3 cut(s) 165, 215, 559
HpaI GTTAAC 1 cut(s) 253
HpaII CCGG 6 cut(s) 61, 352, 371, 470, 569, 604
HphI GGTGA 3 cut(s) 50, 356, 521
Hpy166II GTNNAC 4 cut(s) 45, 80, 253, 563
Hpy188III TCNNGA 2 cut(s) 447, 520
Hpy8I GTNNAC 4 cut(s) 45, 80, 253, 563
Hpy99I CGWCG 2 cut(s) 386, 448
HpyAV CCTTC 2 cut(s) 602, 606
HpyCH4IV ACGT 4 cut(s) 175, 430, 443, 585
HpyF10VI GCNNNNNNNGC 4 cut(s) 272, 307, 311, 333
HpyF3I CTNAG 3 cut(s) 276, 329, 456
HpySE526I ACGT 4 cut(s) 175, 430, 443, 585
HspAI GCGC 1 cut(s) 334
KspAI GTTAAC 1 cut(s) 253
Kzo9I GATC 2 cut(s) 66, 526
LpnPI CCDG 9 cut(s) 74, 83, 287, 296, 365, 384, 483, 582, 617
MaeI CTAG 3 cut(s) 24, 375, 576
MaeII ACGT 4 cut(s) 175, 430, 443, 585
MaeIII GTNAC 4 cut(s) 10, 56, 581, 586
MalI GATC 2 cut(s) 68, 528
MboI GATC 2 cut(s) 66, 526
MhlI GDGCHC 2 cut(s) 411, 513
MlyI GAGTC 1 cut(s) 568
MnlI CCTC 8 cut(s) 11, 38, 94, 143, 316, 379, 603, 609
MroXI GAANNNNTTC 1 cut(s) 245
MseI TTAA 3 cut(s) 252, 290, 357
MspI CCGG 6 cut(s) 61, 352, 371, 470, 569, 604
MspR9I CCNGG 4 cut(s) 371, 470, 570, 605
MvnI CGCG 2 cut(s) 336, 630
MwoI GCNNNNNNNGC 4 cut(s) 272, 307, 311, 333
NciI CCSGG 4 cut(s) 371, 470, 570, 605
NdeII GATC 2 cut(s) 66, 526
NheI GCTAGC 1 cut(s) 374
NlaIV GGNNCC 4 cut(s) 286, 474, 511, 638
NmeAIII GCCGAG 2 cut(s) 331, 574
NmuCI GTSAC 2 cut(s) 56, 586
PaeR7I CTCGAG 1 cut(s) 98
PdmI GAANNNNTTC 1 cut(s) 245
PfeI GAWTC 2 cut(s) 165, 215
PflFI GACNNNGTC 1 cut(s) 40
PleI GAGTC 1 cut(s) 567
PpsI GAGTC 1 cut(s) 567
Ppu21I YACGTR 2 cut(s) 176, 586
PpuMI RGGWCCY 1 cut(s) 285
Psp5II RGGWCCY 1 cut(s) 285
PspEI GGTNACC 1 cut(s) 56
PspN4I GGNNCC 4 cut(s) 286, 474, 511, 638
PspOMI GGGCCC 1 cut(s) 509
PspPI GGNCC 6 cut(s) 130, 285, 325, 472, 509, 510
PspPPI RGGWCCY 1 cut(s) 285
PspXI VCTCGAGB 1 cut(s) 98
PsyI GACNNNGTC 1 cut(s) 40
RsaI GTAC 1 cut(s) 178
RsaNI GTAC 1 cut(s) 177
SaqAI TTAA 3 cut(s) 252, 290, 357
Sau3AI GATC 2 cut(s) 66, 526
Sau96I GGNCC 6 cut(s) 130, 285, 325, 472, 509, 510
SchI GAGTC 1 cut(s) 568
ScrFI CCNGG 4 cut(s) 371, 470, 570, 605
SduI GDGCHC 2 cut(s) 411, 513
Sfr274I CTCGAG 1 cut(s) 98
SinI GGWCC 1 cut(s) 285
SlaI CTCGAG 1 cut(s) 98
SmlI CTYRAG 2 cut(s) 98, 414
SmoI CTYRAG 2 cut(s) 98, 414
SnaBI TACGTA 1 cut(s) 176
SsiI CCGC 9 cut(s) 54, 75, 262, 266, 273, 282, 336, 579, 628
SspMI CTAG 3 cut(s) 24, 375, 576
StyD4I CCNGG 4 cut(s) 369, 468, 568, 603
TaiI ACGT 4 cut(s) 178, 433, 446, 588
TaqI TCGA 5 cut(s) 35, 99, 163, 425, 446
TfiI GAWTC 2 cut(s) 165, 215
Tru1I TTAA 3 cut(s) 252, 290, 357
Tru9I TTAA 3 cut(s) 252, 290, 357
TscAI CASTG 1 cut(s) 77
TseFI GTSAC 2 cut(s) 56, 586
Tsp45I GTSAC 2 cut(s) 56, 586
TspDTI ATGAA 2 cut(s) 157, 246
TspGWI ACGGA 2 cut(s) 62, 161
TspRI CASTG 1 cut(s) 77
Tth111I GACNNNGTC 1 cut(s) 40
VpaK11BI GGWCC 1 cut(s) 285
XagI CCTNNNNNAGG 1 cut(s) 292
XcmI CCANNNNNNNNNTGG 1 cut(s) 520
XhoI CTCGAG 1 cut(s) 98
XmiI GTMKAC 2 cut(s) 44, 79
XmnI GAANNNNTTC 1 cut(s) 245
XspI CTAG 3 cut(s) 24, 375, 576
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.