Rw2G021730

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0001756
Physical Location & Seq
Forward (+)
30230210 .. 30234333
4124 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G021730.1

Sequence Viewer

Length: 552 bp
ATGGATTTCCATGTTCATCGGCTTATGAACAGTGCCGGCTCCAGCCGAGCCGTATTGTCAAAAGTCTATTGTACCCCTGCCTCCTGTCTCTTGCGTCGACACCTGGGTGTTCTCAGTGCTATCGTGTCTTTGCTTCTTCGTTGCAGAGAGGTGGAGAGAGAGAGCGAGGAAAGTGGTGATTTCTTGGACAGTTGGGTTGTTGCTCAGCAGTGTGTGTATTTCTTGCTTCAACACCTAGGTGTTCTCAGTGCTATCGTGTCTATGATACCTCGCTACACCGAGAGGGGTTTTTGGGTTATTAGCTTAACCTTGAGGTGGAGGTGTGTTGACAAAGGCAGCAGAGCTGCTGAGATTGCTTCTACATTCAAGGTTTCATATGCTACTGTTGTAAGAAATTGGTCTGCAAGGTCTGAAGAGAAGGAGAAAGTTTGGGCATGCAATTGTTTTGAGCAGCAGATGAATGGCAACCAAAAAGATGAAGTGATTTGCAAAGCAAGCGAAAAGATGAAGTGGTCTGCCAGTGATATGATTCTGCGAACAGTGTGCAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

183

Amino Acids

20.85

Weight (kDa)

8.76

Isoelectric Point (pI)

43.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 97
AcuI CTGAAG 1 cut(s) 432
AfaI GTAC 1 cut(s) 73
AfiI CCNNNNNNNGG 1 cut(s) 315
AgsI TTSAA 2 cut(s) 230, 367
AjnI CCWGG 1 cut(s) 102
AleI CACNNNNGTG 2 cut(s) 105, 237
AluBI AGCT 2 cut(s) 303, 344
AluI AGCT 2 cut(s) 303, 344
Alw26I GTCTC 1 cut(s) 92
ApeKI GCWGC 3 cut(s) 336, 344, 451
AspA2I CCTAGG 1 cut(s) 235
AsuHPI GGTGA 1 cut(s) 188
AvrII CCTAGG 1 cut(s) 235
BbvI GCAGC 3 cut(s) 331, 348, 463
BceAI ACGGC 1 cut(s) 35
BcgI CGANNNNNNTGC 1 cut(s) 525
BciT130I CCWGG 1 cut(s) 104
BcoDI GTCTC 1 cut(s) 92
BfaI CTAG 1 cut(s) 236
BisI GCNGC 3 cut(s) 337, 345, 452
BlnI CCTAGG 1 cut(s) 235
BlpI GCTNAGC 1 cut(s) 204
BlsI GCNGC 3 cut(s) 338, 346, 453
Bme1390I CCNGG 1 cut(s) 104
BmiI GGNNCC 1 cut(s) 40
BmrFI CCNGG 1 cut(s) 104
BpmI CTGGAG 1 cut(s) 25
Bpu1102I GCTNAGC 1 cut(s) 204
BpuEI CTTGAG 1 cut(s) 331
BsaJI CCNNGG 2 cut(s) 103, 235
Bsc4I CCNNNNNNNGG 1 cut(s) 315
Bse118I RCCGGY 1 cut(s) 35
Bse1I ACTGG 1 cut(s) 519
BseBI CCWGG 1 cut(s) 104
BseDI CCNNGG 2 cut(s) 103, 235
BseLI CCNNNNNNNGG 1 cut(s) 315
BseMII CTCAG 4 cut(s) 127, 218, 259, 339
BseNI ACTGG 1 cut(s) 519
BseXI GCAGC 3 cut(s) 331, 348, 463
BsiSI CCGG 1 cut(s) 36
BslI CCNNNNNNNGG 1 cut(s) 315
BsmAI GTCTC 1 cut(s) 92
Bsp1720I GCTNAGC 1 cut(s) 204
BspCNI CTCAG 4 cut(s) 126, 217, 258, 340
BspLI GGNNCC 1 cut(s) 40
BsrFI RCCGGY 1 cut(s) 35
BsrI ACTGG 1 cut(s) 519
BssAI RCCGGY 1 cut(s) 35
BssECI CCNNGG 2 cut(s) 103, 235
BssT1I CCWWGG 1 cut(s) 235
Bst2UI CCWGG 1 cut(s) 104
Bst4CI ACNGT 4 cut(s) 32, 191, 385, 541
Bst6I CTCTTC 1 cut(s) 408
BstC8I GCNNGC 3 cut(s) 37, 436, 496
BstDEI CTNAG 4 cut(s) 113, 204, 245, 348
BstMAI GTCTC 1 cut(s) 92
BstMWI GCNNNNNNNGC 2 cut(s) 353, 495
BstNI CCWGG 1 cut(s) 104
BstNSI RCATGY 1 cut(s) 438
BstSCI CCNGG 1 cut(s) 102
BstV1I GCAGC 3 cut(s) 331, 348, 463
BtsI GCAGTG 1 cut(s) 215
BtsIMutI CAGTG 6 cut(s) 37, 121, 215, 253, 526, 546
Cac8I GCNNGC 3 cut(s) 37, 436, 496
Cfr10I RCCGGY 1 cut(s) 35
CseI GACGC 1 cut(s) 83
Csp6I GTAC 1 cut(s) 72
CviAII CATG 2 cut(s) 11, 435
CviJI RGCY 6 cut(s) 22, 39, 45, 50, 303, 344
CviKI_1 RGCY 6 cut(s) 22, 39, 45, 50, 303, 344
CviQI GTAC 1 cut(s) 72
DdeI CTNAG 4 cut(s) 113, 204, 245, 348
Eam1104I CTCTTC 1 cut(s) 408
EarI CTCTTC 1 cut(s) 408
Eco130I CCWWGG 1 cut(s) 235
Eco57I CTGAAG 1 cut(s) 432
EcoRII CCWGG 1 cut(s) 102
EcoT14I CCWWGG 1 cut(s) 235
ErhI CCWWGG 1 cut(s) 235
FaeI CATG 2 cut(s) 14, 438
FaiI YATR 7 cut(s) 12, 26, 263, 376, 378, 436, 527
FatI CATG 2 cut(s) 10, 434
FauNDI CATATG 1 cut(s) 376
FblI GTMKAC 1 cut(s) 97
Fnu4HI GCNGC 3 cut(s) 337, 345, 452
Fsp4HI GCNGC 3 cut(s) 337, 345, 452
FspBI CTAG 1 cut(s) 236
GluI GCNGC 3 cut(s) 337, 345, 452
GsuI CTGGAG 1 cut(s) 25
HapII CCGG 1 cut(s) 36
HgaI GACGC 1 cut(s) 83
Hin1II CATG 2 cut(s) 14, 438
HincII GTYRAC 2 cut(s) 98, 328
HindII GTYRAC 2 cut(s) 98, 328
HinfI GANTC 1 cut(s) 529
HpaII CCGG 1 cut(s) 36
HphI GGTGA 1 cut(s) 188
Hpy166II GTNNAC 2 cut(s) 98, 328
Hpy188I TCNGA 1 cut(s) 412
Hpy8I GTNNAC 2 cut(s) 98, 328
Hpy99I CGWCG 1 cut(s) 99
HpyAV CCTTC 1 cut(s) 412
HpyCH4III ACNGT 4 cut(s) 32, 191, 385, 541
HpyCH4V TGCA 5 cut(s) 144, 404, 438, 489, 546
HpyF10VI GCNNNNNNNGC 2 cut(s) 353, 495
HpyF3I CTNAG 4 cut(s) 113, 204, 245, 348
Hsp92II CATG 2 cut(s) 14, 438
KroI GCCGGC 1 cut(s) 35
KroNI GCCGGC 1 cut(s) 37
LmnI GCTCC 1 cut(s) 44
LpnPI CCDG 7 cut(s) 49, 55, 89, 90, 97, 116, 532
Lsp1109I GCAGC 3 cut(s) 331, 348, 463
MaeI CTAG 1 cut(s) 236
MboII GAAGA 2 cut(s) 128, 425
MfeI CAATTG 1 cut(s) 439
MluCI AATT 2 cut(s) 394, 439
MnlI CCTC 7 cut(s) 91, 142, 160, 276, 279, 306, 312
MroNI GCCGGC 1 cut(s) 35
MseI TTAA 1 cut(s) 305
MslI CAYNNNNRTG 2 cut(s) 105, 237
MspI CCGG 1 cut(s) 36
MspR9I CCNGG 1 cut(s) 104
MunI CAATTG 1 cut(s) 439
MvaI CCWGG 1 cut(s) 104
MwoI GCNNNNNNNGC 2 cut(s) 353, 495
NaeI GCCGGC 1 cut(s) 37
NdeI CATATG 1 cut(s) 376
NgoMIV GCCGGC 1 cut(s) 35
NlaIII CATG 2 cut(s) 14, 438
NlaIV GGNNCC 1 cut(s) 40
NmeAIII GCCGAG 1 cut(s) 71
NspI RCATGY 1 cut(s) 438
OliI CACNNNNGTG 2 cut(s) 105, 237
PaeI GCATGC 1 cut(s) 438
PdiI GCCGGC 1 cut(s) 37
PfeI GAWTC 1 cut(s) 529
PkrI GCNGC 3 cut(s) 338, 346, 453
Psp6I CCWGG 1 cut(s) 102
PspGI CCWGG 1 cut(s) 102
PspN4I GGNNCC 1 cut(s) 40
RsaI GTAC 1 cut(s) 73
RsaNI GTAC 1 cut(s) 72
RseI CAYNNNNRTG 2 cut(s) 105, 237
SalI GTCGAC 1 cut(s) 96
SaqAI TTAA 1 cut(s) 305
SatI GCNGC 3 cut(s) 337, 345, 452
ScrFI CCNGG 1 cut(s) 104
SmiMI CAYNNNNRTG 2 cut(s) 105, 237
SmlI CTYRAG 1 cut(s) 310
SmoI CTYRAG 1 cut(s) 310
SphI GCATGC 1 cut(s) 438
Sse9I AATT 2 cut(s) 394, 439
SspMI CTAG 1 cut(s) 236
StyD4I CCNGG 1 cut(s) 102
StyI CCWWGG 1 cut(s) 235
TaaI ACNGT 4 cut(s) 32, 191, 385, 541
TaqI TCGA 1 cut(s) 97
TasI AATT 2 cut(s) 394, 439
TfiI GAWTC 1 cut(s) 529
Tru1I TTAA 1 cut(s) 305
Tru9I TTAA 1 cut(s) 305
TscAI CASTG 6 cut(s) 37, 121, 215, 253, 526, 546
TseI GCWGC 3 cut(s) 336, 344, 451
TspDTI ATGAA 6 cut(s) 5, 41, 363, 473, 492, 521
TspRI CASTG 6 cut(s) 37, 121, 215, 253, 526, 546
XceI RCATGY 1 cut(s) 438
XmaJI CCTAGG 1 cut(s) 235
XmiI GTMKAC 1 cut(s) 97
XspI CTAG 1 cut(s) 236
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.