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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 1 cut(s) | 68 |
| AsuHPI | GGTGA | 2 cut(s) | 124, 164 |
| BbsI | GAAGAC | 1 cut(s) | 63 |
| BccI | CCATC | 4 cut(s) | 131, 152, 191, 197 |
| BpiI | GAAGAC | 1 cut(s) | 63 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 198, 228, 271, 301 |
| BseGI | GGATG | 1 cut(s) | 255 |
| BseRI | GAGGAG | 1 cut(s) | 292 |
| Bsh1236I | CGCG | 1 cut(s) | 68 |
| BsiSI | CCGG | 1 cut(s) | 9 |
| BslFI | GGGAC | 1 cut(s) | 67 |
| BsmFI | GGGAC | 1 cut(s) | 67 |
| BspFNI | CGCG | 1 cut(s) | 68 |
| Bst6I | CTCTTC | 2 cut(s) | 221, 303 |
| BstF5I | GGATG | 1 cut(s) | 255 |
| BstFNI | CGCG | 1 cut(s) | 68 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 74 |
| BstUI | CGCG | 1 cut(s) | 68 |
| BstV2I | GAAGAC | 1 cut(s) | 63 |
| BtsCI | GGATG | 1 cut(s) | 255 |
| CseI | GACGC | 1 cut(s) | 57 |
| DraI | TTTAAA | 1 cut(s) | 291 |
| Eam1104I | CTCTTC | 2 cut(s) | 221, 303 |
| EarI | CTCTTC | 2 cut(s) | 221, 303 |
| FaiI | YATR | 2 cut(s) | 104, 125 |
| FaqI | GGGAC | 1 cut(s) | 67 |
| FokI | GGATG | 1 cut(s) | 262 |
| HapII | CCGG | 1 cut(s) | 9 |
| HgaI | GACGC | 1 cut(s) | 57 |
| HincII | GTYRAC | 1 cut(s) | 42 |
| HindII | GTYRAC | 1 cut(s) | 42 |
| HinfI | GANTC | 2 cut(s) | 63, 80 |
| HpaII | CCGG | 1 cut(s) | 9 |
| HphI | GGTGA | 2 cut(s) | 124, 164 |
| Hpy166II | GTNNAC | 1 cut(s) | 42 |
| Hpy188I | TCNGA | 1 cut(s) | 239 |
| Hpy8I | GTNNAC | 1 cut(s) | 42 |
| HpyAV | CCTTC | 2 cut(s) | 211, 247 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 74 |
| LpnPI | CCDG | 1 cut(s) | 22 |
| MboII | GAAGA | 4 cut(s) | 63, 146, 238, 311 |
| MluCI | AATT | 1 cut(s) | 257 |
| MlyI | GAGTC | 1 cut(s) | 89 |
| MnlI | CCTC | 3 cut(s) | 28, 200, 270 |
| MseI | TTAA | 1 cut(s) | 290 |
| MspI | CCGG | 1 cut(s) | 9 |
| MvnI | CGCG | 1 cut(s) | 68 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 74 |
| NmeAIII | GCCGAG | 1 cut(s) | 103 |
| PfeI | GAWTC | 1 cut(s) | 63 |
| PleI | GAGTC | 1 cut(s) | 88 |
| PpsI | GAGTC | 1 cut(s) | 88 |
| SaqAI | TTAA | 1 cut(s) | 290 |
| SchI | GAGTC | 1 cut(s) | 89 |
| SetI | ASST | 1 cut(s) | 211 |
| SgeI | CNNG | 4 cut(s) | 21, 25, 79, 91 |
| Sse9I | AATT | 1 cut(s) | 257 |
| TasI | AATT | 1 cut(s) | 257 |
| TfiI | GAWTC | 1 cut(s) | 63 |
| Tru1I | TTAA | 1 cut(s) | 290 |
| Tru9I | TTAA | 1 cut(s) | 290 |
| TspDTI | ATGAA | 4 cut(s) | 17, 131, 239, 266 |