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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 1 cut(s) | 235 |
| AccIII | TCCGGA | 2 cut(s) | 82, 303 |
| AciI | CCGC | 1 cut(s) | 92 |
| AclWI | GGATC | 3 cut(s) | 44, 80, 93 |
| AdeI | CACNNNGTG | 1 cut(s) | 235 |
| AfaI | GTAC | 2 cut(s) | 212, 240 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 163, 235 |
| AgsI | TTSAA | 2 cut(s) | 245, 265 |
| AluBI | AGCT | 1 cut(s) | 199 |
| AluI | AGCT | 1 cut(s) | 199 |
| AlwI | GGATC | 3 cut(s) | 44, 80, 93 |
| Aor13HI | TCCGGA | 2 cut(s) | 82, 303 |
| AoxI | GGCC | 1 cut(s) | 109 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 23 |
| AsuC2I | CCSGG | 1 cut(s) | 157 |
| AsuHPI | GGTGA | 2 cut(s) | 35, 93 |
| BaeGI | GKGCMC | 1 cut(s) | 131 |
| BamHI | GGATCC | 1 cut(s) | 85 |
| BccI | CCATC | 1 cut(s) | 212 |
| BcnI | CCSGG | 1 cut(s) | 157 |
| BfaI | CTAG | 1 cut(s) | 275 |
| Bme1390I | CCNGG | 1 cut(s) | 157 |
| BmiI | GGNNCC | 1 cut(s) | 87 |
| BmrFI | CCNGG | 1 cut(s) | 157 |
| BpuMI | CCSGG | 1 cut(s) | 157 |
| BsaJI | CCNNGG | 2 cut(s) | 106, 155 |
| BsaWI | WCCGGW | 2 cut(s) | 82, 303 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 163, 235 |
| BseAI | TCCGGA | 2 cut(s) | 82, 303 |
| BseDI | CCNNGG | 2 cut(s) | 106, 155 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 163, 235 |
| BseSI | GKGCMC | 1 cut(s) | 131 |
| BshFI | GGCC | 1 cut(s) | 111 |
| BsiSI | CCGG | 5 cut(s) | 83, 89, 117, 157, 304 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 163, 235 |
| BsmI | GAATGC | 3 cut(s) | 167, 278, 299 |
| BsnI | GGCC | 1 cut(s) | 111 |
| Bsp1286I | GDGCHC | 1 cut(s) | 131 |
| Bsp13I | TCCGGA | 2 cut(s) | 82, 303 |
| Bsp143I | GATC | 2 cut(s) | 36, 85 |
| BspACI | CCGC | 1 cut(s) | 92 |
| BspANI | GGCC | 1 cut(s) | 111 |
| BspEI | TCCGGA | 2 cut(s) | 82, 303 |
| BspLI | GGNNCC | 1 cut(s) | 87 |
| BspPI | GGATC | 3 cut(s) | 44, 80, 93 |
| BssECI | CCNNGG | 2 cut(s) | 106, 155 |
| BssMI | GATC | 2 cut(s) | 36, 85 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 139 |
| BstC8I | GCNNGC | 1 cut(s) | 131 |
| BstKTI | GATC | 2 cut(s) | 39, 88 |
| BstMBI | GATC | 2 cut(s) | 36, 85 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 139 |
| BstSCI | CCNGG | 1 cut(s) | 155 |
| BstSLI | GKGCMC | 1 cut(s) | 131 |
| BstX2I | RGATCY | 1 cut(s) | 85 |
| BstYI | RGATCY | 1 cut(s) | 85 |
| BsuRI | GGCC | 1 cut(s) | 111 |
| Cac8I | GCNNGC | 1 cut(s) | 131 |
| Csp6I | GTAC | 2 cut(s) | 211, 239 |
| CviAII | CATG | 1 cut(s) | 233 |
| CviJI | RGCY | 3 cut(s) | 111, 199, 228 |
| CviKI_1 | RGCY | 3 cut(s) | 111, 199, 228 |
| CviQI | GTAC | 2 cut(s) | 211, 239 |
| DpnI | GATC | 2 cut(s) | 38, 87 |
| DpnII | GATC | 2 cut(s) | 36, 85 |
| DraIII | CACNNNGTG | 1 cut(s) | 235 |
| FaeI | CATG | 1 cut(s) | 236 |
| FaiI | YATR | 6 cut(s) | 29, 50, 98, 192, 234, 310 |
| FatI | CATG | 1 cut(s) | 232 |
| FspBI | CTAG | 1 cut(s) | 275 |
| HaeIII | GGCC | 1 cut(s) | 111 |
| HapII | CCGG | 5 cut(s) | 83, 89, 117, 157, 304 |
| Hin1II | CATG | 1 cut(s) | 236 |
| HinfI | GANTC | 1 cut(s) | 73 |
| HpaII | CCGG | 5 cut(s) | 83, 89, 117, 157, 304 |
| HphI | GGTGA | 2 cut(s) | 35, 93 |
| Hpy188III | TCNNGA | 3 cut(s) | 34, 83, 304 |
| HpyCH4V | TGCA | 1 cut(s) | 142 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 139 |
| Hsp92II | CATG | 1 cut(s) | 236 |
| Kpn2I | TCCGGA | 2 cut(s) | 82, 303 |
| Kzo9I | GATC | 2 cut(s) | 36, 85 |
| LpnPI | CCDG | 4 cut(s) | 96, 102, 130, 170 |
| MaeI | CTAG | 1 cut(s) | 275 |
| MaeIII | GTNAC | 2 cut(s) | 41, 99 |
| MalI | GATC | 2 cut(s) | 38, 87 |
| MboI | GATC | 2 cut(s) | 36, 85 |
| MboII | GAAGA | 2 cut(s) | 132, 274 |
| MflI | RGATCY | 1 cut(s) | 85 |
| MhlI | GDGCHC | 1 cut(s) | 131 |
| MluCI | AATT | 1 cut(s) | 257 |
| MlyI | GAGTC | 1 cut(s) | 82 |
| MnlI | CCTC | 5 cut(s) | 63, 116, 122, 157, 188 |
| MroI | TCCGGA | 2 cut(s) | 82, 303 |
| MroXI | GAANNNNTTC | 1 cut(s) | 23 |
| MspI | CCGG | 5 cut(s) | 83, 89, 117, 157, 304 |
| MspR9I | CCNGG | 1 cut(s) | 157 |
| Mva1269I | GAATGC | 3 cut(s) | 167, 278, 299 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 139 |
| NciI | CCSGG | 1 cut(s) | 157 |
| NdeII | GATC | 2 cut(s) | 36, 85 |
| NlaIII | CATG | 1 cut(s) | 236 |
| NlaIV | GGNNCC | 1 cut(s) | 87 |
| NmeAIII | GCCGAG | 1 cut(s) | 87 |
| NmuCI | GTSAC | 2 cut(s) | 41, 99 |
| PctI | GAATGC | 3 cut(s) | 167, 278, 299 |
| PdmI | GAANNNNTTC | 1 cut(s) | 23 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 235 |
| PleI | GAGTC | 1 cut(s) | 81 |
| PpsI | GAGTC | 1 cut(s) | 81 |
| PspN4I | GGNNCC | 1 cut(s) | 87 |
| PsuI | RGATCY | 1 cut(s) | 85 |
| RsaI | GTAC | 2 cut(s) | 212, 240 |
| RsaNI | GTAC | 2 cut(s) | 211, 239 |
| Sau3AI | GATC | 2 cut(s) | 36, 85 |
| SchI | GAGTC | 1 cut(s) | 82 |
| ScrFI | CCNGG | 1 cut(s) | 157 |
| SduI | GDGCHC | 1 cut(s) | 131 |
| SetI | ASST | 3 cut(s) | 55, 201, 292 |
| Sse9I | AATT | 1 cut(s) | 257 |
| SsiI | CCGC | 1 cut(s) | 92 |
| SspMI | CTAG | 1 cut(s) | 275 |
| StyD4I | CCNGG | 1 cut(s) | 155 |
| TasI | AATT | 1 cut(s) | 257 |
| TseFI | GTSAC | 2 cut(s) | 41, 99 |
| Tsp45I | GTSAC | 2 cut(s) | 41, 99 |
| TspDTI | ATGAA | 2 cut(s) | 16, 17 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 235 |
| XmnI | GAANNNNTTC | 1 cut(s) | 23 |
| XspI | CTAG | 1 cut(s) | 275 |