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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AfaI | GTAC | 1 cut(s) | 38 |
| AgsI | TTSAA | 1 cut(s) | 157 |
| AluBI | AGCT | 1 cut(s) | 45 |
| AluI | AGCT | 1 cut(s) | 45 |
| BccI | CCATC | 1 cut(s) | 107 |
| BfmI | CTRYAG | 1 cut(s) | 40 |
| BmcAI | AGTACT | 1 cut(s) | 38 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 108, 138 |
| BseYI | CCCAGC | 1 cut(s) | 45 |
| Bst4CI | ACNGT | 1 cut(s) | 171 |
| BstSFI | CTRYAG | 1 cut(s) | 40 |
| Csp6I | GTAC | 1 cut(s) | 37 |
| CviAII | CATG | 1 cut(s) | 59 |
| CviJI | RGCY | 2 cut(s) | 45, 55 |
| CviKI_1 | RGCY | 2 cut(s) | 45, 55 |
| CviQI | GTAC | 1 cut(s) | 37 |
| FaeI | CATG | 1 cut(s) | 62 |
| FaiI | YATR | 5 cut(s) | 27, 60, 79, 88, 149 |
| FatI | CATG | 1 cut(s) | 58 |
| GsaI | CCCAGC | 1 cut(s) | 49 |
| Hin1II | CATG | 1 cut(s) | 62 |
| HpyAV | CCTTC | 1 cut(s) | 20 |
| HpyCH4III | ACNGT | 1 cut(s) | 171 |
| Hsp92II | CATG | 1 cut(s) | 62 |
| LpnPI | CCDG | 2 cut(s) | 31, 149 |
| MnlI | CCTC | 2 cut(s) | 98, 110 |
| MslI | CAYNNNNRTG | 1 cut(s) | 63 |
| MspA1I | CMGCKG | 1 cut(s) | 45 |
| NlaIII | CATG | 1 cut(s) | 62 |
| PspFI | CCCAGC | 1 cut(s) | 45 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 23, 55 |
| PvuII | CAGCTG | 1 cut(s) | 45 |
| RsaI | GTAC | 1 cut(s) | 38 |
| RsaNI | GTAC | 1 cut(s) | 37 |
| RseI | CAYNNNNRTG | 1 cut(s) | 63 |
| ScaI | AGTACT | 1 cut(s) | 38 |
| SetI | ASST | 3 cut(s) | 47, 109, 121 |
| SfcI | CTRYAG | 1 cut(s) | 40 |
| SgeI | CNNG | 5 cut(s) | 46, 58, 64, 71, 134 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 63 |
| TaaI | ACNGT | 1 cut(s) | 171 |
| TatI | WGTACW | 1 cut(s) | 36 |
| ZrmI | AGTACT | 1 cut(s) | 38 |