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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcsI | RAATTY | 1 cut(s) | 17 |
| AfaI | GTAC | 1 cut(s) | 26 |
| ApoI | RAATTY | 1 cut(s) | 17 |
| ArsI | GACNNNNNNTTYG | 3 cut(s) | 34, 53, 85 |
| BmrI | ACTGGG | 1 cut(s) | 51 |
| BmuI | ACTGGG | 1 cut(s) | 51 |
| Bse1I | ACTGG | 2 cut(s) | 46, 144 |
| Bse3DI | GCAATG | 1 cut(s) | 13 |
| BseMI | GCAATG | 1 cut(s) | 13 |
| BseNI | ACTGG | 2 cut(s) | 46, 144 |
| BsrDI | GCAATG | 1 cut(s) | 13 |
| BsrI | ACTGG | 2 cut(s) | 46, 144 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 120 |
| BtsIMutI | CAGTG | 1 cut(s) | 151 |
| Csp6I | GTAC | 1 cut(s) | 25 |
| CviAII | CATG | 1 cut(s) | 130 |
| CviQI | GTAC | 1 cut(s) | 25 |
| FaeI | CATG | 1 cut(s) | 133 |
| FaiI | YATR | 2 cut(s) | 131, 152 |
| FatI | CATG | 1 cut(s) | 129 |
| Hin1II | CATG | 1 cut(s) | 133 |
| HpyAV | CCTTC | 1 cut(s) | 28 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 120 |
| Hsp92II | CATG | 1 cut(s) | 133 |
| LpnPI | CCDG | 2 cut(s) | 27, 62 |
| MfeI | CAATTG | 1 cut(s) | 64 |
| MluCI | AATT | 2 cut(s) | 17, 64 |
| MnlI | CCTC | 2 cut(s) | 109, 126 |
| MunI | CAATTG | 1 cut(s) | 64 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 120 |
| NlaIII | CATG | 1 cut(s) | 133 |
| RsaI | GTAC | 1 cut(s) | 26 |
| RsaNI | GTAC | 1 cut(s) | 25 |
| SetI | ASST | 1 cut(s) | 137 |
| SgeI | CNNG | 5 cut(s) | 43, 54, 89, 127, 142 |
| Sse9I | AATT | 2 cut(s) | 17, 64 |
| TaqII | GACCGA | 1 cut(s) | 27 |
| TasI | AATT | 2 cut(s) | 17, 64 |
| TscAI | CASTG | 1 cut(s) | 151 |
| TspDTI | ATGAA | 3 cut(s) | 69, 98, 117 |
| TspRI | CASTG | 1 cut(s) | 151 |
| XapI | RAATTY | 1 cut(s) | 17 |