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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 365 |
| AccB1I | GGYRCC | 1 cut(s) | 365 |
| AciI | CCGC | 1 cut(s) | 212 |
| AcsI | RAATTY | 1 cut(s) | 319 |
| AfaI | GTAC | 1 cut(s) | 367 |
| AgsI | TTSAA | 2 cut(s) | 189, 325 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 424 |
| AluBI | AGCT | 1 cut(s) | 388 |
| AluI | AGCT | 1 cut(s) | 388 |
| ApoI | RAATTY | 1 cut(s) | 319 |
| Asp718I | GGTACC | 1 cut(s) | 365 |
| AspS9I | GGNCC | 1 cut(s) | 242 |
| AsuHPI | GGTGA | 4 cut(s) | 47, 65, 127, 164 |
| AsuNHI | GCTAGC | 1 cut(s) | 257 |
| AvaII | GGWCC | 1 cut(s) | 242 |
| BanI | GGYRCC | 1 cut(s) | 365 |
| BccI | CCATC | 6 cut(s) | 7, 26, 92, 112, 134, 155 |
| BfaI | CTAG | 1 cut(s) | 258 |
| BisI | GCNGC | 1 cut(s) | 213 |
| BlsI | GCNGC | 1 cut(s) | 214 |
| Bme18I | GGWCC | 1 cut(s) | 242 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 424 |
| BmgT120I | GGNCC | 1 cut(s) | 242 |
| BmiI | GGNNCC | 1 cut(s) | 367 |
| BmsI | GCATC | 2 cut(s) | 57, 225 |
| BmtI | GCTAGC | 1 cut(s) | 261 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 93, 123 |
| Bse118I | RCCGGY | 1 cut(s) | 368 |
| BseGI | GGATG | 2 cut(s) | 18, 22 |
| BseRI | GAGGAG | 5 cut(s) | 509, 512, 515, 518, 521 |
| BshNI | GGYRCC | 1 cut(s) | 365 |
| BsiSI | CCGG | 1 cut(s) | 369 |
| BspACI | CCGC | 1 cut(s) | 212 |
| BspHI | TCATGA | 1 cut(s) | 448 |
| BspLI | GGNNCC | 1 cut(s) | 367 |
| BspOI | GCTAGC | 1 cut(s) | 261 |
| BspT107I | GGYRCC | 1 cut(s) | 365 |
| BsrFI | RCCGGY | 1 cut(s) | 368 |
| BssAI | RCCGGY | 1 cut(s) | 368 |
| Bst6I | CTCTTC | 3 cut(s) | 474, 480, 486 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 235 |
| BstC8I | GCNNGC | 3 cut(s) | 259, 390, 432 |
| BstF5I | GGATG | 2 cut(s) | 18, 22 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 76, 235, 263, 436 |
| BstNSI | RCATGY | 1 cut(s) | 311 |
| BtsCI | GGATG | 2 cut(s) | 18, 22 |
| BtsI | GCAGTG | 1 cut(s) | 222 |
| BtsIMutI | CAGTG | 1 cut(s) | 222 |
| Cac8I | GCNNGC | 3 cut(s) | 259, 390, 432 |
| CciI | TCATGA | 1 cut(s) | 448 |
| Cfr10I | RCCGGY | 1 cut(s) | 368 |
| Cfr13I | GGNCC | 1 cut(s) | 242 |
| Csp6I | GTAC | 1 cut(s) | 366 |
| CviAII | CATG | 4 cut(s) | 239, 263, 308, 449 |
| CviJI | RGCY | 6 cut(s) | 79, 261, 278, 372, 388, 434 |
| CviKI_1 | RGCY | 6 cut(s) | 79, 261, 278, 372, 388, 434 |
| CviQI | GTAC | 1 cut(s) | 366 |
| DriI | GACNNNNNGTC | 1 cut(s) | 424 |
| Eam1104I | CTCTTC | 3 cut(s) | 474, 480, 486 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 424 |
| EarI | CTCTTC | 3 cut(s) | 474, 480, 486 |
| Eco47I | GGWCC | 1 cut(s) | 242 |
| EcoT22I | ATGCAT | 1 cut(s) | 240 |
| FaeI | CATG | 4 cut(s) | 242, 266, 311, 452 |
| FaiI | YATR | 6 cut(s) | 65, 223, 240, 264, 309, 450 |
| FatI | CATG | 4 cut(s) | 238, 262, 307, 448 |
| Fnu4HI | GCNGC | 1 cut(s) | 213 |
| FokI | GGATG | 2 cut(s) | 25, 29 |
| Fsp4HI | GCNGC | 1 cut(s) | 213 |
| FspBI | CTAG | 1 cut(s) | 258 |
| GluI | GCNGC | 1 cut(s) | 213 |
| HapII | CCGG | 1 cut(s) | 369 |
| Hin1II | CATG | 4 cut(s) | 242, 266, 311, 452 |
| HindIII | AAGCTT | 1 cut(s) | 386 |
| HinfI | GANTC | 3 cut(s) | 380, 406, 464 |
| HpaII | CCGG | 1 cut(s) | 369 |
| HphI | GGTGA | 4 cut(s) | 47, 65, 127, 164 |
| Hpy188III | TCNNGA | 3 cut(s) | 29, 325, 449 |
| HpyAV | CCTTC | 1 cut(s) | 322 |
| HpyCH4V | TGCA | 2 cut(s) | 229, 238 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 76, 235, 263, 436 |
| Hsp92II | CATG | 4 cut(s) | 242, 266, 311, 452 |
| KpnI | GGTACC | 1 cut(s) | 369 |
| LmnI | GCTCC | 1 cut(s) | 75 |
| LpnPI | CCDG | 5 cut(s) | 274, 348, 378, 382, 416 |
| LweI | GCATC | 2 cut(s) | 57, 225 |
| MaeI | CTAG | 1 cut(s) | 258 |
| MluCI | AATT | 2 cut(s) | 319, 413 |
| MlyI | GAGTC | 2 cut(s) | 374, 400 |
| Mph1103I | ATGCAT | 1 cut(s) | 240 |
| MseI | TTAA | 1 cut(s) | 126 |
| MspI | CCGG | 1 cut(s) | 369 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 76, 235, 263, 436 |
| NheI | GCTAGC | 1 cut(s) | 257 |
| NlaIII | CATG | 4 cut(s) | 242, 266, 311, 452 |
| NlaIV | GGNNCC | 1 cut(s) | 367 |
| NsiI | ATGCAT | 1 cut(s) | 240 |
| NspI | RCATGY | 1 cut(s) | 311 |
| PagI | TCATGA | 1 cut(s) | 448 |
| PfeI | GAWTC | 1 cut(s) | 464 |
| PkrI | GCNGC | 1 cut(s) | 214 |
| PleI | GAGTC | 2 cut(s) | 374, 400 |
| PpsI | GAGTC | 2 cut(s) | 374, 400 |
| PspN4I | GGNNCC | 1 cut(s) | 367 |
| PspPI | GGNCC | 1 cut(s) | 242 |
| RsaI | GTAC | 1 cut(s) | 367 |
| RsaNI | GTAC | 1 cut(s) | 366 |
| SaqAI | TTAA | 1 cut(s) | 126 |
| SatI | GCNGC | 1 cut(s) | 213 |
| Sau96I | GGNCC | 1 cut(s) | 242 |
| SchI | GAGTC | 2 cut(s) | 374, 400 |
| SetI | ASST | 5 cut(s) | 132, 293, 299, 367, 390 |
| SfaNI | GCATC | 2 cut(s) | 57, 225 |
| SinI | GGWCC | 1 cut(s) | 242 |
| Sse9I | AATT | 2 cut(s) | 319, 413 |
| SsiI | CCGC | 1 cut(s) | 212 |
| SspMI | CTAG | 1 cut(s) | 258 |
| TaqI | TCGA | 1 cut(s) | 383 |
| TasI | AATT | 2 cut(s) | 319, 413 |
| TauI | GCSGC | 1 cut(s) | 215 |
| TfiI | GAWTC | 1 cut(s) | 464 |
| Tru1I | TTAA | 1 cut(s) | 126 |
| Tru9I | TTAA | 1 cut(s) | 126 |
| TscAI | CASTG | 1 cut(s) | 222 |
| TspDTI | ATGAA | 4 cut(s) | 36, 57, 72, 465 |
| TspRI | CASTG | 1 cut(s) | 222 |
| VpaK11BI | GGWCC | 1 cut(s) | 242 |
| XapI | RAATTY | 1 cut(s) | 319 |
| XceI | RCATGY | 1 cut(s) | 311 |
| XspI | CTAG | 1 cut(s) | 258 |
| Zsp2I | ATGCAT | 1 cut(s) | 240 |