RchiOBHm_Chr7g0201931

60S ribosomal protein L23

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0001168
Physical Location & Seq
Forward (+)
19556425 .. 19556908
484 bp
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UTR
Exon/CDS
Intron
PRQ18075

Sequence Viewer

Length: 483 bp
ATGATATCATGCAAACAAAGACTCAATTTTGACATATGCCATCAAGTTGGATCTCAGACCATGTCGAAGCGAGGGCGCGGAGGGTCTGCCGGAAACAAGTTCCGGATGTCGCTGGGTCTGCCCGTGGCGGATACGGTGAACTGCGCCGACAACACCGGTGCCAAGAATCTCTACATTATCTCTGTGAAGGGAATCAAGGGTCGTCTGAACCGGTTGCCGTCGGCTTGTGTGGGTGACATGGTCATGGCCACTGTGAAGAAAGGAAAGCCGGACCTCAGGAAGAAGGTGCTGCCAGCTGTCATTGTGAGGCAGCGCAAGCCGTGGCGCCGAAAGGATGGTGTTTTCATGTACTTCGAAGATAATGCTGGTGTCATTGTGAATCCAAAGGGTGAAATGAAAGGATCTGCTATCACTGGGCCTATTGGGAAGGAATGCGCTGATCTGTGGCCAAGAATTGCTAGTGCAGCTAATGCCATAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L14

Protein Analysis

160

Amino Acids

17.33

Weight (kDa)

10.21

Isoelectric Point (pI)

31.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L14 PF00238 42 - 160 6.3e-41 Ribosomal protein L14p/L23e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 158, 324
AccII CGCG 1 cut(s) 78
AccIII TCCGGA 1 cut(s) 102
AciI CCGC 2 cut(s) 78, 128
AclWI GGATC 2 cut(s) 58, 409
AcoI YGGCCR 2 cut(s) 246, 446
AcyI GRCGYC 1 cut(s) 325
AfaI GTAC 1 cut(s) 350
AgeI ACCGGT 2 cut(s) 155, 210
AluBI AGCT 2 cut(s) 296, 467
AluI AGCT 2 cut(s) 296, 467
AlwI GGATC 2 cut(s) 58, 409
Aor13HI TCCGGA 1 cut(s) 102
AoxI GGCC 3 cut(s) 246, 416, 446
ApeKI GCWGC 3 cut(s) 289, 310, 464
AsiGI ACCGGT 2 cut(s) 155, 210
AspLEI GCGC 5 cut(s) 78, 146, 315, 327, 437
AspS9I GGNCC 2 cut(s) 271, 416
AsuHPI GGTGA 3 cut(s) 148, 245, 401
AsuII TTCGAA 1 cut(s) 354
AvaII GGWCC 1 cut(s) 271
AxyI CCTNAGG 1 cut(s) 275
BalI TGGCCA 2 cut(s) 248, 448
BanI GGYRCC 2 cut(s) 158, 324
BbvI GCAGC 3 cut(s) 276, 322, 476
BccI CCATC 2 cut(s) 48, 329
BceAI ACGGC 2 cut(s) 202, 304
BcgI CGANNNNNNTGC 2 cut(s) 344, 378
BciVI GTATCC 1 cut(s) 124
BfaI CTAG 1 cut(s) 459
BfoI RGCGCY 1 cut(s) 328
BfuI GTATCC 1 cut(s) 124
BisI GCNGC 3 cut(s) 290, 311, 465
BlsI GCNGC 3 cut(s) 291, 312, 466
Bme18I GGWCC 1 cut(s) 271
BmgT120I GGNCC 2 cut(s) 271, 416
BmiI GGNNCC 2 cut(s) 160, 326
BmrI ACTGGG 1 cut(s) 423
BmuI ACTGGG 1 cut(s) 423
Bpu14I TTCGAA 1 cut(s) 354
BsaHI GRCGYC 1 cut(s) 325
BsaJI CCNNGG 2 cut(s) 123, 320
BsaWI WCCGGW 3 cut(s) 102, 155, 210
Bse118I RCCGGY 2 cut(s) 155, 210
Bse1I ACTGG 1 cut(s) 418
Bse21I CCTNAGG 1 cut(s) 275
BseAI TCCGGA 1 cut(s) 102
BseDI CCNNGG 2 cut(s) 123, 320
BseGI GGATG 2 cut(s) 111, 340
BseMII CTCAG 2 cut(s) 68, 289
BseNI ACTGG 1 cut(s) 418
BseXI GCAGC 3 cut(s) 276, 322, 476
BseYI CCCAGC 1 cut(s) 112
BsgI GTGCAG 1 cut(s) 483
Bsh1236I CGCG 1 cut(s) 78
BshFI GGCC 3 cut(s) 248, 418, 448
BshNI GGYRCC 2 cut(s) 158, 324
BshTI ACCGGT 2 cut(s) 155, 210
BsiSI CCGG 5 cut(s) 90, 103, 156, 211, 269
BsmI GAATGC 1 cut(s) 437
BsnI GGCC 3 cut(s) 248, 418, 448
Bsp119I TTCGAA 1 cut(s) 354
Bsp13I TCCGGA 1 cut(s) 102
Bsp143I GATC 3 cut(s) 50, 401, 439
BspACI CCGC 2 cut(s) 78, 128
BspANI GGCC 3 cut(s) 248, 418, 448
BspCNI CTCAG 2 cut(s) 67, 288
BspEI TCCGGA 1 cut(s) 102
BspFNI CGCG 1 cut(s) 78
BspLI GGNNCC 2 cut(s) 160, 326
BspPI GGATC 2 cut(s) 58, 409
BspT104I TTCGAA 1 cut(s) 354
BspT107I GGYRCC 2 cut(s) 158, 324
BsrFI RCCGGY 2 cut(s) 155, 210
BsrI ACTGG 1 cut(s) 418
BssAI RCCGGY 2 cut(s) 155, 210
BssECI CCNNGG 2 cut(s) 123, 320
BssMI GATC 3 cut(s) 50, 401, 439
BssNI GRCGYC 1 cut(s) 325
Bst4CI ACNGT 2 cut(s) 136, 253
BstACI GRCGYC 1 cut(s) 325
BstAPI GCANNNNNTGC 1 cut(s) 470
BstBI TTCGAA 1 cut(s) 354
BstC8I GCNNGC 2 cut(s) 294, 317
BstDEI CTNAG 2 cut(s) 54, 275
BstDSI CCRYGG 2 cut(s) 123, 320
BstF5I GGATG 2 cut(s) 111, 340
BstFNI CGCG 1 cut(s) 78
BstH2I RGCGCY 1 cut(s) 328
BstHHI GCGC 5 cut(s) 78, 146, 315, 327, 437
BstKTI GATC 3 cut(s) 53, 404, 442
BstMBI GATC 3 cut(s) 50, 401, 439
BstMWI GCNNNNNNNGC 4 cut(s) 118, 316, 464, 470
BstUI CGCG 1 cut(s) 78
BstV1I GCAGC 3 cut(s) 276, 322, 476
BstX2I RGATCY 2 cut(s) 50, 401
BstXI CCANNNNNNTGG 1 cut(s) 47
BstYI RGATCY 2 cut(s) 50, 401
Bsu36I CCTNAGG 1 cut(s) 275
BsuI GTATCC 1 cut(s) 124
BsuRI GGCC 3 cut(s) 248, 418, 448
BtgI CCRYGG 2 cut(s) 123, 320
BtsCI GGATG 2 cut(s) 111, 340
BtsIMutI CAGTG 2 cut(s) 249, 411
Cac8I GCNNGC 2 cut(s) 294, 317
CfoI GCGC 5 cut(s) 78, 146, 315, 327, 437
Cfr10I RCCGGY 2 cut(s) 155, 210
Cfr13I GGNCC 2 cut(s) 271, 416
Csp6I GTAC 1 cut(s) 349
CspAI ACCGGT 2 cut(s) 155, 210
CviAII CATG 5 cut(s) 9, 61, 238, 244, 346
CviJI RGCY 8 cut(s) 224, 248, 268, 296, 319, 418, 448, 467
CviKI_1 RGCY 8 cut(s) 224, 248, 268, 296, 319, 418, 448, 467
CviQI GTAC 1 cut(s) 349
DdeI CTNAG 2 cut(s) 54, 275
DinI GGCGCC 1 cut(s) 326
DpnI GATC 3 cut(s) 52, 403, 441
DpnII GATC 3 cut(s) 50, 401, 439
EaeI YGGCCR 2 cut(s) 246, 446
EciI GGCGGA 1 cut(s) 143
Eco32I GATATC 1 cut(s) 6
Eco47I GGWCC 1 cut(s) 271
Eco81I CCTNAGG 1 cut(s) 275
EcoRV GATATC 1 cut(s) 6
EgeI GGCGCC 1 cut(s) 326
EheI GGCGCC 1 cut(s) 326
FaeI CATG 5 cut(s) 12, 64, 241, 247, 349
FaiI YATR 8 cut(s) 10, 35, 37, 62, 239, 245, 347, 476
FatI CATG 5 cut(s) 8, 60, 237, 243, 345
FauNDI CATATG 1 cut(s) 35
Fnu4HI GCNGC 3 cut(s) 290, 311, 465
FokI GGATG 2 cut(s) 118, 347
Fsp4HI GCNGC 3 cut(s) 290, 311, 465
FspBI CTAG 1 cut(s) 459
GlaI GCGC 5 cut(s) 77, 145, 314, 326, 436
GluI GCNGC 3 cut(s) 290, 311, 465
GsaI CCCAGC 1 cut(s) 116
HaeII RGCGCY 1 cut(s) 328
HaeIII GGCC 3 cut(s) 248, 418, 448
HapII CCGG 5 cut(s) 90, 103, 156, 211, 269
HhaI GCGC 5 cut(s) 78, 146, 315, 327, 437
Hin1I GRCGYC 1 cut(s) 325
Hin1II CATG 5 cut(s) 12, 64, 241, 247, 349
Hin6I GCGC 5 cut(s) 76, 144, 313, 325, 435
HinP1I GCGC 5 cut(s) 76, 144, 313, 325, 435
HinfI GANTC 4 cut(s) 21, 166, 192, 379
HpaII CCGG 5 cut(s) 90, 103, 156, 211, 269
HphI GGTGA 3 cut(s) 148, 245, 401
Hpy166II GTNNAC 1 cut(s) 139
Hpy188I TCNGA 2 cut(s) 57, 207
Hpy188III TCNNGA 2 cut(s) 103, 277
Hpy8I GTNNAC 1 cut(s) 139
Hpy99I CGWCG 1 cut(s) 223
HpyAV CCTTC 3 cut(s) 181, 277, 421
HpyCH4III ACNGT 2 cut(s) 136, 253
HpyCH4V TGCA 2 cut(s) 12, 464
HpyF10VI GCNNNNNNNGC 4 cut(s) 118, 316, 464, 470
HpyF3I CTNAG 2 cut(s) 54, 275
Hsp92I GRCGYC 1 cut(s) 325
Hsp92II CATG 5 cut(s) 12, 64, 241, 247, 349
HspAI GCGC 5 cut(s) 76, 144, 313, 325, 435
KasI GGCGCC 1 cut(s) 324
Kpn2I TCCGGA 1 cut(s) 102
Kzo9I GATC 3 cut(s) 50, 401, 439
Lsp1109I GCAGC 3 cut(s) 276, 322, 476
MaeI CTAG 1 cut(s) 459
MaeIII GTNAC 1 cut(s) 233
MalI GATC 3 cut(s) 52, 403, 441
MboI GATC 3 cut(s) 50, 401, 439
MboII GAAGA 3 cut(s) 268, 292, 368
MflI RGATCY 2 cut(s) 50, 401
MlsI TGGCCA 2 cut(s) 248, 448
MluCI AATT 2 cut(s) 25, 453
MluNI TGGCCA 2 cut(s) 248, 448
Mly113I GGCGCC 1 cut(s) 325
MlyI GAGTC 1 cut(s) 15
MmeI TCCRAC 1 cut(s) 28
MnlI CCTC 4 cut(s) 65, 74, 284, 300
Mox20I TGGCCA 2 cut(s) 248, 448
MroI TCCGGA 1 cut(s) 102
MscI TGGCCA 2 cut(s) 248, 448
MseI TTAA 1 cut(s) 481
MslI CAYNNNNRTG 1 cut(s) 242
Msp20I TGGCCA 2 cut(s) 248, 448
MspA1I CMGCKG 1 cut(s) 296
MspI CCGG 5 cut(s) 90, 103, 156, 211, 269
Mva1269I GAATGC 1 cut(s) 437
MvnI CGCG 1 cut(s) 78
MwoI GCNNNNNNNGC 4 cut(s) 118, 316, 464, 470
NarI GGCGCC 1 cut(s) 325
NdeI CATATG 1 cut(s) 35
NdeII GATC 3 cut(s) 50, 401, 439
NlaIII CATG 5 cut(s) 12, 64, 241, 247, 349
NlaIV GGNNCC 2 cut(s) 160, 326
NmuCI GTSAC 1 cut(s) 233
NspV TTCGAA 1 cut(s) 354
PctI GAATGC 1 cut(s) 437
PfeI GAWTC 3 cut(s) 166, 192, 379
PflFI GACNNNGTC 2 cut(s) 61, 239
PinAI ACCGGT 2 cut(s) 155, 210
PkrI GCNGC 3 cut(s) 291, 312, 466
PleI GAGTC 1 cut(s) 15
PluTI GGCGCC 1 cut(s) 328
PpsI GAGTC 1 cut(s) 15
PspFI CCCAGC 1 cut(s) 112
PspN4I GGNNCC 2 cut(s) 160, 326
PspPI GGNCC 2 cut(s) 271, 416
PsuI RGATCY 2 cut(s) 50, 401
PsyI GACNNNGTC 2 cut(s) 61, 239
PvuII CAGCTG 1 cut(s) 296
RsaI GTAC 1 cut(s) 350
RsaNI GTAC 1 cut(s) 349
RseI CAYNNNNRTG 1 cut(s) 242
SaqAI TTAA 1 cut(s) 481
SatI GCNGC 3 cut(s) 290, 311, 465
Sau3AI GATC 3 cut(s) 50, 401, 439
Sau96I GGNCC 2 cut(s) 271, 416
SchI GAGTC 1 cut(s) 15
SetI ASST 4 cut(s) 276, 288, 298, 469
SfoI GGCGCC 1 cut(s) 326
SfuI TTCGAA 1 cut(s) 354
SgrAI CRCCGGYG 1 cut(s) 155
SinI GGWCC 1 cut(s) 271
SmiMI CAYNNNNRTG 1 cut(s) 242
Sse9I AATT 2 cut(s) 25, 453
SsiI CCGC 2 cut(s) 78, 128
SspDI GGCGCC 1 cut(s) 324
SspMI CTAG 1 cut(s) 459
TaaI ACNGT 2 cut(s) 136, 253
TaqI TCGA 2 cut(s) 65, 354
TasI AATT 2 cut(s) 25, 453
TatI WGTACW 1 cut(s) 348
TfiI GAWTC 3 cut(s) 166, 192, 379
Tru1I TTAA 1 cut(s) 481
Tru9I TTAA 1 cut(s) 481
TscAI CASTG 2 cut(s) 256, 418
TseFI GTSAC 1 cut(s) 233
TseI GCWGC 3 cut(s) 289, 310, 464
Tsp45I GTSAC 1 cut(s) 233
TspDTI ATGAA 2 cut(s) 334, 410
TspRI CASTG 2 cut(s) 256, 418
Tth111I GACNNNGTC 2 cut(s) 61, 239
VpaK11BI GGWCC 1 cut(s) 271
XspI CTAG 1 cut(s) 459
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.