Rorug02G0620700

60S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0001168
Physical Location & Seq
Forward (+)
73783431 .. 73784183
753 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0620700.1

Sequence Viewer

Length: 753 bp
ATGACCATCGTGTGCTGCAAAGAACCCGAGGCTTGGGGGAGGACATGTTTCTCGATCATCAGACTAAAGTTGTTCAAGGCAACAGCCAGCCAACTTAACAGCAGTTCTTATGAAGAATATCGTCGAATAGCTGTAAGACGTTATGGGATCAATTTTTCAGATATGAAGCGGTATTTATCATCATCATCATGGAATGAAAACGGGTTTACGCGAAAAATATCTGAAACTCTTACAAGCATGGGTGTCCCGCATGATTTACTGCCGGCTATTTTTCAAACATTGTCTGCGGTGGTTGAGGCGGCTGTCTCTGCCCACCATATTGATGTGGATGTGTGCGAAACCACTTACCTAAGGGACGGGGACGGAGACGGAGTTGGAGGTGGAGTTGGAGTTGGAGTTGGAGGTGGAGGTGGAGAGGTTGGAGGTGTAGGTGAAGGTGTTGGAGGTGGAGGTGGAGGTGGAGGTGTTGGAGGTGGAGGTGTAGGTGTAGGTGGATTGATTCCTGCGAGTGAATCATCAATTGAGGGTTTGGAGCAAGTGACACTTGATAATACTATTATCACTCAGACGCCCTCGTGTGCGATTTGTTTGGAGGATTTTGCAGCTTTTGTAGATGATCAACAAGAACCGACTACGGGGTTGCCCTGCCGACACCACTATCACGTACACTGCATTGTCCAGTGGCTGGAGTTCAGCCACCTATGTCCTCTCTGCCGATACCCAATGCCAACGGAAGATAATCAGTCAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L14

Protein Analysis

250

Amino Acids

26.42

Weight (kDa)

4.93

Isoelectric Point (pI)

45.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 192 - 239 9e-12 Ring finger domain
zf-rbx1 PF12678 192 - 239 1.3e-08 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 685
AccII CGCG 1 cut(s) 211
AciI CCGC 4 cut(s) 169, 248, 287, 299
AclWI GGATC 1 cut(s) 155
AcyI GRCGYC 1 cut(s) 569
AfaI GTAC 1 cut(s) 666
AfiI CCNNNNNNNGG 3 cut(s) 33, 635, 685
AflIII ACRYGT 1 cut(s) 44
AgsI TTSAA 2 cut(s) 76, 275
AluBI AGCT 2 cut(s) 131, 605
AluI AGCT 2 cut(s) 131, 605
Alw26I GTCTC 2 cut(s) 310, 360
AlwI GGATC 1 cut(s) 155
AlwNI CAGNNNCTG 1 cut(s) 685
Ama87I CYCGRG 1 cut(s) 26
ApeKI GCWGC 2 cut(s) 15, 602
AsuHPI GGTGA 1 cut(s) 443
AvaI CYCGRG 1 cut(s) 26
AxyI CCTNAGG 1 cut(s) 350
BauI CACGAG 1 cut(s) 574
BbvI GCAGC 2 cut(s) 2, 614
BccI CCATC 1 cut(s) 14
BclI TGATCA 1 cut(s) 616
BcoDI GTCTC 2 cut(s) 310, 360
BisI GCNGC 3 cut(s) 16, 300, 603
BlsI GCNGC 3 cut(s) 17, 301, 604
BmeT110I CYCGRG 1 cut(s) 26
BpmI CTGGAG 1 cut(s) 707
BsaAI YACGTR 1 cut(s) 664
BsaHI GRCGYC 1 cut(s) 569
BsaJI CCNNGG 1 cut(s) 27
Bsc4I CCNNNNNNNGG 3 cut(s) 33, 635, 685
Bse118I RCCGGY 1 cut(s) 262
Bse1I ACTGG 1 cut(s) 679
Bse21I CCTNAGG 1 cut(s) 350
BseDI CCNNGG 1 cut(s) 27
BseGI GGATG 1 cut(s) 334
BseLI CCNNNNNNNGG 3 cut(s) 33, 635, 685
BseMII CTCAG 1 cut(s) 578
BseNI ACTGG 1 cut(s) 679
BseXI GCAGC 2 cut(s) 2, 614
Bsh1236I CGCG 1 cut(s) 211
BsiHKCI CYCGRG 1 cut(s) 26
BsiSI CCGG 1 cut(s) 263
BslFI GGGAC 3 cut(s) 230, 368, 374
BslI CCNNNNNNNGG 3 cut(s) 33, 635, 685
BsmAI GTCTC 2 cut(s) 310, 360
BsmBI CGTCTC 1 cut(s) 360
BsmFI GGGAC 3 cut(s) 230, 368, 374
BsoBI CYCGRG 1 cut(s) 26
Bsp143I GATC 3 cut(s) 54, 147, 616
BspACI CCGC 4 cut(s) 169, 248, 287, 299
BspCNI CTCAG 1 cut(s) 577
BspFNI CGCG 1 cut(s) 211
BspPI GGATC 1 cut(s) 155
BsrFI RCCGGY 1 cut(s) 262
BsrI ACTGG 1 cut(s) 679
BssAI RCCGGY 1 cut(s) 262
BssECI CCNNGG 1 cut(s) 27
BssMI GATC 3 cut(s) 54, 147, 616
BssNI GRCGYC 1 cut(s) 569
BssSI CACGAG 1 cut(s) 574
Bst2BI CACGAG 1 cut(s) 574
BstACI GRCGYC 1 cut(s) 569
BstBAI YACGTR 1 cut(s) 664
BstC8I GCNNGC 2 cut(s) 88, 264
BstDEI CTNAG 2 cut(s) 350, 564
BstF5I GGATG 1 cut(s) 334
BstFNI CGCG 1 cut(s) 211
BstKTI GATC 3 cut(s) 57, 150, 619
BstMAI GTCTC 2 cut(s) 310, 360
BstMBI GATC 3 cut(s) 54, 147, 616
BstMWI GCNNNNNNNGC 1 cut(s) 308
BstNSI RCATGY 1 cut(s) 48
BstUI CGCG 1 cut(s) 211
BstV1I GCAGC 2 cut(s) 2, 614
Bsu36I CCTNAGG 1 cut(s) 350
BtsCI GGATG 1 cut(s) 334
BtsI GCAGTG 1 cut(s) 667
BtsIMutI CAGTG 2 cut(s) 667, 686
Cac8I GCNNGC 2 cut(s) 88, 264
CaiI CAGNNNCTG 1 cut(s) 685
Cfr10I RCCGGY 1 cut(s) 262
CseI GACGC 1 cut(s) 577
Csp6I GTAC 1 cut(s) 665
CviAII CATG 4 cut(s) 45, 189, 238, 251
CviJI RGCY 9 cut(s) 32, 86, 90, 131, 266, 302, 605, 685, 696
CviKI_1 RGCY 9 cut(s) 32, 86, 90, 131, 266, 302, 605, 685, 696
CviQI GTAC 1 cut(s) 665
DdeI CTNAG 2 cut(s) 350, 564
DpnI GATC 3 cut(s) 56, 149, 618
DpnII GATC 3 cut(s) 54, 147, 616
Eco81I CCTNAGG 1 cut(s) 350
Eco88I CYCGRG 1 cut(s) 26
Esp3I CGTCTC 1 cut(s) 360
FaeI CATG 4 cut(s) 48, 192, 241, 254
FaiI YATR 9 cut(s) 46, 111, 144, 164, 190, 239, 252, 318, 703
FalI AAGNNNNNCTT 2 cut(s) 528, 560
FaqI GGGAC 3 cut(s) 230, 368, 374
FatI CATG 4 cut(s) 44, 188, 237, 250
FauI CCCGC 1 cut(s) 255
FbaI TGATCA 1 cut(s) 616
Fnu4HI GCNGC 3 cut(s) 16, 300, 603
FokI GGATG 1 cut(s) 341
Fsp4HI GCNGC 3 cut(s) 16, 300, 603
GluI GCNGC 3 cut(s) 16, 300, 603
GsuI CTGGAG 1 cut(s) 707
HapII CCGG 1 cut(s) 263
HgaI GACGC 1 cut(s) 577
Hin1I GRCGYC 1 cut(s) 569
Hin1II CATG 4 cut(s) 48, 192, 241, 254
HinfI GANTC 2 cut(s) 499, 512
HpaII CCGG 1 cut(s) 263
HphI GGTGA 1 cut(s) 443
Hpy166II GTNNAC 2 cut(s) 207, 667
Hpy188I TCNGA 4 cut(s) 62, 160, 223, 567
Hpy188III TCNNGA 1 cut(s) 52
Hpy8I GTNNAC 2 cut(s) 207, 667
Hpy99I CGWCG 1 cut(s) 126
HpyAV CCTTC 1 cut(s) 428
HpyCH4IV ACGT 2 cut(s) 139, 663
HpyCH4V TGCA 3 cut(s) 18, 602, 672
HpyF10VI GCNNNNNNNGC 1 cut(s) 308
HpyF3I CTNAG 2 cut(s) 350, 564
HpySE526I ACGT 2 cut(s) 139, 663
Hsp92I GRCGYC 1 cut(s) 569
Hsp92II CATG 4 cut(s) 48, 192, 241, 254
KroI GCCGGC 1 cut(s) 262
KroNI GCCGGC 1 cut(s) 264
Ksp22I TGATCA 1 cut(s) 616
Kzo9I GATC 3 cut(s) 54, 147, 616
LmnI GCTCC 1 cut(s) 532
LpnPI CCDG 6 cut(s) 100, 276, 516, 658, 671, 692
Lsp1109I GCAGC 2 cut(s) 2, 614
MaeII ACGT 2 cut(s) 139, 663
MaeIII GTNAC 1 cut(s) 538
MalI GATC 3 cut(s) 56, 149, 618
MboI GATC 3 cut(s) 54, 147, 616
MboII GAAGA 2 cut(s) 125, 746
MfeI CAATTG 1 cut(s) 519
MluCI AATT 2 cut(s) 151, 519
MmeI TCCRAC 7 cut(s) 355, 367, 373, 379, 400, 421, 448
MroNI GCCGGC 1 cut(s) 262
MseI TTAA 1 cut(s) 96
MslI CAYNNNNRTG 2 cut(s) 187, 321
MspI CCGG 1 cut(s) 263
MunI CAATTG 1 cut(s) 519
MvnI CGCG 1 cut(s) 211
MwoI GCNNNNNNNGC 1 cut(s) 308
NaeI GCCGGC 1 cut(s) 264
NdeII GATC 3 cut(s) 54, 147, 616
NgoMIV GCCGGC 1 cut(s) 262
NlaIII CATG 4 cut(s) 48, 192, 241, 254
NmuCI GTSAC 1 cut(s) 538
NspI RCATGY 1 cut(s) 48
PciI ACATGT 1 cut(s) 44
PdiI GCCGGC 1 cut(s) 264
PfeI GAWTC 2 cut(s) 499, 512
PflMI CCANNNNNTGG 1 cut(s) 685
PkrI GCNGC 3 cut(s) 17, 301, 604
Ppu21I YACGTR 1 cut(s) 664
PscI ACATGT 1 cut(s) 44
PstNI CAGNNNCTG 1 cut(s) 685
RsaI GTAC 1 cut(s) 666
RsaNI GTAC 1 cut(s) 665
RseI CAYNNNNRTG 2 cut(s) 187, 321
SaqAI TTAA 1 cut(s) 96
SatI GCNGC 3 cut(s) 16, 300, 603
Sau3AI GATC 3 cut(s) 54, 147, 616
SmiMI CAYNNNNRTG 2 cut(s) 187, 321
Sse9I AATT 2 cut(s) 151, 519
SsiI CCGC 4 cut(s) 169, 248, 287, 299
TaiI ACGT 2 cut(s) 142, 666
TaqI TCGA 2 cut(s) 53, 124
TasI AATT 2 cut(s) 151, 519
TauI GCSGC 1 cut(s) 302
TfiI GAWTC 2 cut(s) 499, 512
Tru1I TTAA 1 cut(s) 96
Tru9I TTAA 1 cut(s) 96
TscAI CASTG 2 cut(s) 674, 686
TseFI GTSAC 1 cut(s) 538
TseI GCWGC 2 cut(s) 15, 602
Tsp45I GTSAC 1 cut(s) 538
TspDTI ATGAA 3 cut(s) 126, 179, 210
TspGWI ACGGA 3 cut(s) 378, 384, 746
TspRI CASTG 2 cut(s) 674, 686
Van91I CCANNNNNTGG 1 cut(s) 685
XceI RCATGY 1 cut(s) 48
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.