RLG00000003639

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0001256
Physical Location & Seq
Reverse (-)
51099782 .. 51101927
2146 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003639

Sequence Viewer

Length: 459 bp
ATGGCGATGTTTATCGTCAATCACTTTCTCAATCTATGCCAACTCTTTACCCTTGGGGAGAGAGGTGGAAACTACCAAGAAGGAGTCAGCAGCAGACCTCTAAGGACGAGCCTCGTGCGCTTTCCTCCAAGCGGCAACTATGGCTTGCTCCTAGAAGTGAGTAAAGTTTGCGGCAATTGGCGGTATGGTGCTCCTACATTTGCCGTTTCTGATGAATCGGATCTCCGACTTGCCTCAAGATGGGGGTGGATAGGAGGATGGCCCTCGGGTTTGAACTTGAGGAGGCCTTGTTCTGGCGTTATCGAGCTCTGGGATCTGAGGAAAATGGTGAGGCTGGCGGTTCATTGCCGAAGCAAACTTGTAGAGGAAGAAGAGTCTGATGACGGTGGTCGGTCTCGGCCGCGTCGGGGGCGTCTCAAGGTGGACGAAAGTCTTGCTGATTCTCAGTTTAGCGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

153

Amino Acids

17.09

Weight (kDa)

9.09

Isoelectric Point (pI)

59.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 403
AciI CCGC 6 cut(s) 132, 171, 181, 338, 401, 453
AclWI GGATC 2 cut(s) 228, 321
AcoI YGGCCR 1 cut(s) 398
AcyI GRCGYC 1 cut(s) 412
AfiI CCNNNNNNNGG 4 cut(s) 131, 240, 293, 407
AgsI TTSAA 1 cut(s) 274
AloI GAACNNNNNNTCC 2 cut(s) 274, 306
AluBI AGCT 1 cut(s) 307
AluI AGCT 1 cut(s) 307
Alw21I GWGCWC 2 cut(s) 193, 309
Alw26I GTCTC 2 cut(s) 399, 419
AlwI GGATC 2 cut(s) 228, 321
Ama87I CYCGRG 1 cut(s) 265
AoxI GGCC 3 cut(s) 260, 284, 398
ApeKI GCWGC 1 cut(s) 90
AspLEI GCGC 1 cut(s) 120
AspS9I GGNCC 1 cut(s) 261
AsuHPI GGTGA 1 cut(s) 340
AvaI CYCGRG 1 cut(s) 265
BanII GRGCYC 1 cut(s) 309
BauI CACGAG 1 cut(s) 113
Bbv12I GWGCWC 2 cut(s) 193, 309
BbvI GCAGC 1 cut(s) 102
BccI CCATC 2 cut(s) 234, 252
BceAI ACGGC 1 cut(s) 188
BcgI CGANNNNNNTGC 4 cut(s) 97, 131, 416, 450
BcoDI GTCTC 2 cut(s) 399, 419
BfaI CTAG 1 cut(s) 152
BisI GCNGC 4 cut(s) 91, 133, 172, 401
BlsI GCNGC 4 cut(s) 92, 134, 173, 402
BmeT110I CYCGRG 1 cut(s) 265
BmgT120I GGNCC 1 cut(s) 261
BoxI GACNNNNGTC 2 cut(s) 387, 429
BpuEI CTTGAG 3 cut(s) 220, 298, 401
BsaBI GATNNNNATC 1 cut(s) 11
BsaHI GRCGYC 1 cut(s) 412
BsaI GGTCTC 1 cut(s) 399
BsaJI CCNNGG 2 cut(s) 52, 264
BsaXI ACNNNNNCTCC 2 cut(s) 274, 304
Bsc4I CCNNNNNNNGG 4 cut(s) 131, 240, 293, 407
Bse3DI GCAATG 1 cut(s) 343
Bse8I GATNNNNATC 1 cut(s) 11
BseDI CCNNGG 2 cut(s) 52, 264
BseGI GGATG 1 cut(s) 263
BseJI GATNNNNATC 1 cut(s) 11
BseLI CCNNNNNNNGG 4 cut(s) 131, 240, 293, 407
BseMI GCAATG 1 cut(s) 343
BseMII CTCAG 2 cut(s) 308, 458
BseRI GAGGAG 1 cut(s) 295
BseX3I CGGCCG 1 cut(s) 398
BseXI GCAGC 1 cut(s) 102
Bsh1236I CGCG 1 cut(s) 403
Bsh1285I CGRYCG 1 cut(s) 401
BshFI GGCC 3 cut(s) 262, 286, 400
BsiEI CGRYCG 1 cut(s) 401
BsiHKAI GWGCWC 2 cut(s) 193, 309
BsiHKCI CYCGRG 1 cut(s) 265
BslI CCNNNNNNNGG 4 cut(s) 131, 240, 293, 407
BsmAI GTCTC 2 cut(s) 399, 419
BsmBI CGTCTC 1 cut(s) 419
BsnI GGCC 3 cut(s) 262, 286, 400
Bso31I GGTCTC 1 cut(s) 399
BsoBI CYCGRG 1 cut(s) 265
Bsp1286I GDGCHC 2 cut(s) 193, 309
Bsp143I GATC 2 cut(s) 220, 313
BspACI CCGC 6 cut(s) 132, 171, 181, 338, 401, 453
BspANI GGCC 3 cut(s) 262, 286, 400
BspCNI CTCAG 2 cut(s) 309, 457
BspFNI CGCG 1 cut(s) 403
BspPI GGATC 2 cut(s) 228, 321
BspTNI GGTCTC 1 cut(s) 399
BsrDI GCAATG 1 cut(s) 343
BssECI CCNNGG 2 cut(s) 52, 264
BssMI GATC 2 cut(s) 220, 313
BssNI GRCGYC 1 cut(s) 412
BssSI CACGAG 1 cut(s) 113
BssT1I CCWWGG 1 cut(s) 52
Bst2BI CACGAG 1 cut(s) 113
Bst4CI ACNGT 1 cut(s) 386
Bst6I CTCTTC 1 cut(s) 366
BstACI GRCGYC 1 cut(s) 412
BstC8I GCNNGC 2 cut(s) 146, 336
BstDEI CTNAG 3 cut(s) 101, 317, 444
BstF5I GGATG 1 cut(s) 263
BstFNI CGCG 1 cut(s) 403
BstHHI GCGC 1 cut(s) 120
BstKTI GATC 2 cut(s) 223, 316
BstMAI GTCTC 2 cut(s) 399, 419
BstMBI GATC 2 cut(s) 220, 313
BstMCI CGRYCG 1 cut(s) 401
BstMWI GCNNNNNNNGC 3 cut(s) 117, 141, 409
BstPAI GACNNNNGTC 2 cut(s) 387, 429
BstUI CGCG 1 cut(s) 403
BstV1I GCAGC 1 cut(s) 102
BstX2I RGATCY 2 cut(s) 220, 313
BstYI RGATCY 2 cut(s) 220, 313
BstZI CGGCCG 1 cut(s) 398
BsuRI GGCC 3 cut(s) 262, 286, 400
BtgZI GCGATG 1 cut(s) 20
BtsCI GGATG 1 cut(s) 263
Cac8I GCNNGC 2 cut(s) 146, 336
CfoI GCGC 1 cut(s) 120
Cfr13I GGNCC 1 cut(s) 261
CseI GACGC 2 cut(s) 392, 401
CviJI RGCY 7 cut(s) 111, 144, 262, 286, 307, 334, 400
CviKI_1 RGCY 7 cut(s) 111, 144, 262, 286, 307, 334, 400
DdeI CTNAG 3 cut(s) 101, 317, 444
DpnI GATC 2 cut(s) 222, 315
DpnII GATC 2 cut(s) 220, 313
EaeI YGGCCR 1 cut(s) 398
EagI CGGCCG 1 cut(s) 398
Eam1104I CTCTTC 1 cut(s) 366
EarI CTCTTC 1 cut(s) 366
Ecl136II GAGCTC 1 cut(s) 307
EclXI CGGCCG 1 cut(s) 398
Eco130I CCWWGG 1 cut(s) 52
Eco147I AGGCCT 1 cut(s) 286
Eco24I GRGCYC 1 cut(s) 309
Eco31I GGTCTC 1 cut(s) 399
Eco52I CGGCCG 1 cut(s) 398
Eco53kI GAGCTC 1 cut(s) 307
Eco88I CYCGRG 1 cut(s) 265
EcoICRI GAGCTC 1 cut(s) 307
EcoT14I CCWWGG 1 cut(s) 52
EcoT38I GRGCYC 1 cut(s) 309
ErhI CCWWGG 1 cut(s) 52
Esp3I CGTCTC 1 cut(s) 419
FaiI YATR 3 cut(s) 37, 141, 186
Fnu4HI GCNGC 4 cut(s) 91, 133, 172, 401
FokI GGATG 1 cut(s) 270
FriOI GRGCYC 1 cut(s) 309
Fsp4HI GCNGC 4 cut(s) 91, 133, 172, 401
FspBI CTAG 1 cut(s) 152
GlaI GCGC 1 cut(s) 119
GluI GCNGC 4 cut(s) 91, 133, 172, 401
HaeIII GGCC 3 cut(s) 262, 286, 400
HgaI GACGC 2 cut(s) 392, 401
HhaI GCGC 1 cut(s) 120
Hin1I GRCGYC 1 cut(s) 412
Hin6I GCGC 1 cut(s) 118
HinP1I GCGC 1 cut(s) 118
HinfI GANTC 4 cut(s) 84, 215, 374, 440
HphI GGTGA 1 cut(s) 340
Hpy166II GTNNAC 1 cut(s) 424
Hpy188I TCNGA 5 cut(s) 211, 220, 227, 318, 379
Hpy188III TCNNGA 1 cut(s) 237
Hpy8I GTNNAC 1 cut(s) 424
Hpy99I CGWCG 1 cut(s) 408
HpyAV CCTTC 1 cut(s) 74
HpyCH4III ACNGT 1 cut(s) 386
HpyF10VI GCNNNNNNNGC 3 cut(s) 117, 141, 409
HpyF3I CTNAG 3 cut(s) 101, 317, 444
Hsp92I GRCGYC 1 cut(s) 412
HspAI GCGC 1 cut(s) 118
Kzo9I GATC 2 cut(s) 220, 313
LmnI GCTCC 2 cut(s) 153, 196
LpnPI CCDG 3 cut(s) 279, 295, 320
Lsp1109I GCAGC 1 cut(s) 102
MaeI CTAG 1 cut(s) 152
MalI GATC 2 cut(s) 222, 315
MboI GATC 2 cut(s) 220, 313
MboII GAAGA 2 cut(s) 380, 383
MfeI CAATTG 1 cut(s) 175
MflI RGATCY 2 cut(s) 220, 313
MhlI GDGCHC 2 cut(s) 193, 309
MluCI AATT 1 cut(s) 175
MlyI GAGTC 2 cut(s) 93, 383
MmeI TCCRAC 1 cut(s) 250
MunI CAATTG 1 cut(s) 175
MvnI CGCG 1 cut(s) 403
MwoI GCNNNNNNNGC 3 cut(s) 117, 141, 409
NdeII GATC 2 cut(s) 220, 313
NmeAIII GCCGAG 1 cut(s) 376
PceI AGGCCT 1 cut(s) 286
PfeI GAWTC 2 cut(s) 215, 440
PkrI GCNGC 4 cut(s) 92, 134, 173, 402
PleI GAGTC 2 cut(s) 92, 382
PpsI GAGTC 2 cut(s) 92, 382
PshAI GACNNNNGTC 2 cut(s) 387, 429
Psp124BI GAGCTC 1 cut(s) 309
PspPI GGNCC 1 cut(s) 261
PsuI RGATCY 2 cut(s) 220, 313
SacI GAGCTC 1 cut(s) 309
SatI GCNGC 4 cut(s) 91, 133, 172, 401
Sau3AI GATC 2 cut(s) 220, 313
Sau96I GGNCC 1 cut(s) 261
SchI GAGTC 2 cut(s) 93, 383
SduI GDGCHC 2 cut(s) 193, 309
SetI ASST 4 cut(s) 67, 100, 309, 423
SmlI CTYRAG 3 cut(s) 235, 277, 416
SmoI CTYRAG 3 cut(s) 235, 277, 416
Sse9I AATT 1 cut(s) 175
SseBI AGGCCT 1 cut(s) 286
SsiI CCGC 6 cut(s) 132, 171, 181, 338, 401, 453
SspMI CTAG 1 cut(s) 152
SstI GAGCTC 1 cut(s) 309
StuI AGGCCT 1 cut(s) 286
StyI CCWWGG 1 cut(s) 52
TaaI ACNGT 1 cut(s) 386
TaqI TCGA 1 cut(s) 303
TaqII GACCGA 1 cut(s) 381
TasI AATT 1 cut(s) 175
TauI GCSGC 3 cut(s) 135, 174, 403
TfiI GAWTC 2 cut(s) 215, 440
TseI GCWGC 1 cut(s) 90
TspDTI ATGAA 2 cut(s) 228, 332
XspI CTAG 1 cut(s) 152
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.