RLG00000007148
RLK Family

Cysteine-rich RLK (RECEPTOR-like protein kinase) 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0003285
Physical Location & Seq
Reverse (-)
14047510 .. 14049863
2354 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000007148

Sequence Viewer

Length: 855 bp
ATGGTCGCTTCCTCCTACCTGGAAACCCCAGACGTCCTCCTTTCATCCATATGCAATCTCATGCCCATCCGCCTCGAATCCACCAACTACTTCATCTGGTCTCATCTCATGACCACCATCTTCAACGCCCACGGCCTCCGCGGCTACGTTGATGGCCCCATCAAAACCCCTGACAAATTCACCCTCAACGCCCGAGGAGAGCCGACCTCGCAGCTCACCTCCGAGTTCAAGCTTTGGCGCCGCTACGACGTCAGAGTCAAGGTTGTCATCAACGCCACGATGTCCTCGTCGGTGCTACCGCACATCATCGACTCCAACACGCTCACCTCACATCGCAAGCAATCACAACTCCTTCTTCGATTCTCAATAGCTTTGATTTCTAGGGTTTATGATTTGGTTGAGGTGAGGCCATGGAGACTGGCTCGAAGGGAGAGAAGACGAGAGCTCAGGCTCAGGAAGCTGGGGGAGACATTCAGGAAGGACGGGCCAATACTGGGCTTGGAGTTTGATCTGTTGCCACCGGGTGCATTTGGTTCCGTCGTCATCAACGCCATGCTATCCTCGTCGACGCAACAGCACATCATCGGCTCCGACACGTCCAGGGACCTGTGGCAGATGCTGGAGCGGCGATTCACTCTTGTTCGGGTTTCGGAGACACCAGATTTCCGATCCTCCTCCGCCGATCCGTACCAGTTTCCGAGCTTCAGCGTCTGCAAATTTAATCAGAATCCTAAACCCAAAAAAGCCCGCATTCTTTTGAGTCACGGAGCTTCGGATACGGTGCTCTCCTCCCCCATTGCAGGACCCAGAAACCAGAATTCAATAAAAACACAAGATTGTGAAATGTTGAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 Retrotran_gag_2 gag_pre-integrs rve

Protein Analysis

285

Amino Acids

32.31

Weight (kDa)

9.91

Isoelectric Point (pI)

46.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 254
AatII GACGTC 2 cut(s) 36, 252
AccB1I GGYRCC 1 cut(s) 237
AccBSI CCGCTC 1 cut(s) 625
AccI GTMKAC 1 cut(s) 566
AccII CGCG 1 cut(s) 141
AciI CCGC 8 cut(s) 70, 139, 141, 241, 299, 625, 678, 748
AclWI GGATC 2 cut(s) 663, 677
AcsI RAATTY 3 cut(s) 176, 716, 817
AcuI CTGAAG 1 cut(s) 688
AcyI GRCGYC 3 cut(s) 33, 238, 249
AdeI CACNNNGTG 1 cut(s) 524
AfaI GTAC 1 cut(s) 689
AfiI CCNNNNNNNGG 2 cut(s) 494, 800
AflIII ACRYGT 1 cut(s) 594
AgsI TTSAA 3 cut(s) 124, 229, 822
AjiI CACGTC 1 cut(s) 597
AjnI CCWGG 2 cut(s) 18, 599
AluBI AGCT 8 cut(s) 214, 232, 371, 445, 460, 702, 770, 852
AluI AGCT 8 cut(s) 214, 232, 371, 445, 460, 702, 770, 852
Alw21I GWGCWC 2 cut(s) 447, 786
Alw26I GTCTC 4 cut(s) 105, 409, 461, 647
AlwI GGATC 2 cut(s) 663, 677
AlwNI CAGNNNCTG 2 cut(s) 619, 711
Ama87I CYCGRG 1 cut(s) 192
AoxI GGCC 4 cut(s) 133, 154, 407, 485
ApeKI GCWGC 1 cut(s) 211
ApoI RAATTY 3 cut(s) 176, 716, 817
AspLEI GCGC 1 cut(s) 240
AspS9I GGNCC 4 cut(s) 155, 485, 604, 803
AsuC2I CCSGG 1 cut(s) 522
AsuHPI GGTGA 4 cut(s) 172, 208, 316, 415
AvaI CYCGRG 1 cut(s) 192
AvaII GGWCC 2 cut(s) 604, 803
BanI GGYRCC 1 cut(s) 237
BanII GRGCYC 1 cut(s) 447
BbsI GAAGAC 1 cut(s) 442
Bbv12I GWGCWC 2 cut(s) 447, 786
BbvI GCAGC 1 cut(s) 223
BccI CCATC 4 cut(s) 74, 125, 146, 167
BceAI ACGGC 1 cut(s) 148
BciT130I CCWGG 2 cut(s) 20, 601
BciVI GTATCC 1 cut(s) 769
BcnI CCSGG 1 cut(s) 522
BcoDI GTCTC 4 cut(s) 105, 409, 461, 647
BfaI CTAG 1 cut(s) 381
BfoI RGCGCY 1 cut(s) 241
BfuI GTATCC 1 cut(s) 769
BglI GCCNNNNNGGC 1 cut(s) 141
BisI GCNGC 4 cut(s) 142, 212, 241, 626
BlsI GCNGC 4 cut(s) 143, 213, 242, 627
Bme1390I CCNGG 3 cut(s) 20, 522, 601
Bme18I GGWCC 2 cut(s) 604, 803
BmeT110I CYCGRG 1 cut(s) 192
BmgBI CACGTC 1 cut(s) 597
BmgT120I GGNCC 4 cut(s) 155, 485, 604, 803
BmiI GGNNCC 6 cut(s) 157, 239, 535, 589, 605, 805
BmrFI CCNGG 3 cut(s) 20, 522, 601
BmrI ACTGGG 1 cut(s) 503
BmsI GCATC 1 cut(s) 606
BmuI ACTGGG 1 cut(s) 503
BpiI GAAGAC 1 cut(s) 442
BplI GAGNNNNNCTC 4 cut(s) 191, 223, 406, 438
BpmI CTGGAG 1 cut(s) 641
Bpu10I CCTNAGC 2 cut(s) 446, 452
BpuMI CCSGG 1 cut(s) 522
BsaHI GRCGYC 3 cut(s) 33, 238, 249
BsaI GGTCTC 1 cut(s) 105
BsaJI CCNNGG 5 cut(s) 130, 139, 193, 410, 600
Bsc4I CCNNNNNNNGG 2 cut(s) 494, 800
Bse1I ACTGG 3 cut(s) 423, 498, 691
Bse3DI GCAATG 1 cut(s) 795
BseBI CCWGG 2 cut(s) 20, 601
BseDI CCNNGG 5 cut(s) 130, 139, 193, 410, 600
BseGI GGATG 2 cut(s) 44, 66
BseLI CCNNNNNNNGG 2 cut(s) 494, 800
BseMI GCAATG 1 cut(s) 795
BseMII CTCAG 2 cut(s) 460, 466
BseNI ACTGG 3 cut(s) 423, 498, 691
BseRI GAGGAG 3 cut(s) 210, 664, 778
BseXI GCAGC 1 cut(s) 223
BseYI CCCAGC 1 cut(s) 460
Bsh1236I CGCG 1 cut(s) 141
BshFI GGCC 4 cut(s) 135, 156, 409, 487
BshNI GGYRCC 1 cut(s) 237
BsiHKAI GWGCWC 2 cut(s) 447, 786
BsiHKCI CYCGRG 1 cut(s) 192
BsiSI CCGG 1 cut(s) 521
BslFI GGGAC 1 cut(s) 617
BslI CCNNNNNNNGG 2 cut(s) 494, 800
BsmAI GTCTC 4 cut(s) 105, 409, 461, 647
BsmFI GGGAC 1 cut(s) 617
BsmI GAATGC 1 cut(s) 750
BsnI GGCC 4 cut(s) 135, 156, 409, 487
Bso31I GGTCTC 1 cut(s) 105
BsoBI CYCGRG 1 cut(s) 192
Bsp1286I GDGCHC 2 cut(s) 447, 786
Bsp143I GATC 3 cut(s) 508, 668, 682
Bsp19I CCATGG 1 cut(s) 410
BspACI CCGC 8 cut(s) 70, 139, 141, 241, 299, 625, 678, 748
BspANI GGCC 4 cut(s) 135, 156, 409, 487
BspCNI CTCAG 2 cut(s) 459, 465
BspFNI CGCG 1 cut(s) 141
BspHI TCATGA 1 cut(s) 108
BspLI GGNNCC 6 cut(s) 157, 239, 535, 589, 605, 805
BspPI GGATC 2 cut(s) 663, 677
BspT107I GGYRCC 1 cut(s) 237
BspTNI GGTCTC 1 cut(s) 105
BsrBI CCGCTC 1 cut(s) 625
BsrDI GCAATG 1 cut(s) 795
BsrI ACTGG 3 cut(s) 423, 498, 691
BssECI CCNNGG 5 cut(s) 130, 139, 193, 410, 600
BssMI GATC 3 cut(s) 508, 668, 682
BssNI GRCGYC 3 cut(s) 33, 238, 249
BssT1I CCWWGG 1 cut(s) 410
Bst2UI CCWGG 2 cut(s) 20, 601
Bst4CI ACNGT 1 cut(s) 781
BstACI GRCGYC 3 cut(s) 33, 238, 249
BstC8I GCNNGC 2 cut(s) 338, 748
BstDEI CTNAG 2 cut(s) 446, 452
BstDSI CCRYGG 3 cut(s) 130, 139, 410
BstF5I GGATG 2 cut(s) 44, 66
BstFNI CGCG 1 cut(s) 141
BstH2I RGCGCY 1 cut(s) 241
BstHHI GCGC 1 cut(s) 240
BstKTI GATC 3 cut(s) 511, 671, 685
BstMAI GTCTC 4 cut(s) 105, 409, 461, 647
BstMBI GATC 3 cut(s) 508, 668, 682
BstMWI GCNNNNNNNGC 4 cut(s) 141, 208, 457, 625
BstNI CCWGG 2 cut(s) 20, 601
BstSCI CCNGG 3 cut(s) 18, 520, 599
BstUI CGCG 1 cut(s) 141
BstV1I GCAGC 1 cut(s) 223
BstV2I GAAGAC 1 cut(s) 442
BsuI GTATCC 1 cut(s) 769
BsuRI GGCC 4 cut(s) 135, 156, 409, 487
BtgI CCRYGG 3 cut(s) 130, 139, 410
BtgZI GCGATG 1 cut(s) 317
BtrI CACGTC 1 cut(s) 597
BtsCI GGATG 2 cut(s) 44, 66
Cac8I GCNNGC 2 cut(s) 338, 748
CaiI CAGNNNCTG 2 cut(s) 619, 711
CciI TCATGA 1 cut(s) 108
CfoI GCGC 1 cut(s) 240
Cfr13I GGNCC 4 cut(s) 155, 485, 604, 803
Cfr42I CCGCGG 1 cut(s) 142
CseI GACGC 2 cut(s) 577, 697
Csp6I GTAC 1 cut(s) 688
CviAII CATG 4 cut(s) 61, 109, 411, 553
CviQI GTAC 1 cut(s) 688
DdeI CTNAG 2 cut(s) 446, 452
DinI GGCGCC 1 cut(s) 239
DpnI GATC 3 cut(s) 510, 670, 684
DpnII GATC 3 cut(s) 508, 668, 682
DraIII CACNNNGTG 1 cut(s) 524
DrdI GACNNNNNNGTC 1 cut(s) 254
DseDI GACNNNNNNGTC 1 cut(s) 254
EciI GGCGGA 2 cut(s) 59, 667
Ecl136II GAGCTC 1 cut(s) 445
Eco130I CCWWGG 1 cut(s) 410
Eco24I GRGCYC 1 cut(s) 447
Eco31I GGTCTC 1 cut(s) 105
Eco47I GGWCC 2 cut(s) 604, 803
Eco53kI GAGCTC 1 cut(s) 445
Eco57I CTGAAG 1 cut(s) 688
Eco88I CYCGRG 1 cut(s) 192
EcoICRI GAGCTC 1 cut(s) 445
EcoO109I RGGNCCY 2 cut(s) 604, 803
EcoRI GAATTC 1 cut(s) 817
EcoRII CCWGG 2 cut(s) 18, 599
EcoT14I CCWWGG 1 cut(s) 410
EcoT38I GRGCYC 1 cut(s) 447
EgeI GGCGCC 1 cut(s) 239
EheI GGCGCC 1 cut(s) 239
ErhI CCWWGG 1 cut(s) 410
FaeI CATG 4 cut(s) 64, 112, 414, 556
FaiI YATR 7 cut(s) 50, 52, 62, 110, 390, 412, 554
FaqI GGGAC 1 cut(s) 617
FatI CATG 4 cut(s) 60, 108, 410, 552
FauI CCCGC 1 cut(s) 755
FauNDI CATATG 1 cut(s) 50
FblI GTMKAC 1 cut(s) 566
Fnu4HI GCNGC 4 cut(s) 142, 212, 241, 626
FokI GGATG 2 cut(s) 31, 53
FriOI GRGCYC 1 cut(s) 447
Fsp4HI GCNGC 4 cut(s) 142, 212, 241, 626
FspBI CTAG 1 cut(s) 381
GlaI GCGC 1 cut(s) 239
GluI GCNGC 4 cut(s) 142, 212, 241, 626
GsaI CCCAGC 1 cut(s) 464
GsuI CTGGAG 1 cut(s) 641
HaeII RGCGCY 1 cut(s) 241
HaeIII GGCC 4 cut(s) 135, 156, 409, 487
HapII CCGG 1 cut(s) 521
HgaI GACGC 2 cut(s) 577, 697
HhaI GCGC 1 cut(s) 240
Hin1I GRCGYC 3 cut(s) 33, 238, 249
Hin1II CATG 4 cut(s) 64, 112, 414, 556
Hin6I GCGC 1 cut(s) 238
HinP1I GCGC 1 cut(s) 238
HincII GTYRAC 1 cut(s) 567
HindII GTYRAC 1 cut(s) 567
HindIII AAGCTT 1 cut(s) 230
HinfI GANTC 7 cut(s) 77, 255, 311, 360, 630, 727, 760
HpaII CCGG 1 cut(s) 521
HphI GGTGA 4 cut(s) 172, 208, 316, 415
Hpy166II GTNNAC 1 cut(s) 567
Hpy188I TCNGA 8 cut(s) 223, 254, 592, 652, 668, 699, 726, 775
Hpy188III TCNNGA 3 cut(s) 109, 454, 475
Hpy8I GTNNAC 1 cut(s) 567
Hpy99I CGWCG 5 cut(s) 251, 292, 542, 568, 571
HpyAV CCTTC 3 cut(s) 362, 420, 472
HpyCH4III ACNGT 1 cut(s) 781
HpyCH4IV ACGT 4 cut(s) 33, 147, 249, 596
HpyCH4V TGCA 4 cut(s) 54, 527, 714, 800
HpyF10VI GCNNNNNNNGC 4 cut(s) 141, 208, 457, 625
HpyF3I CTNAG 2 cut(s) 446, 452
HpySE526I ACGT 4 cut(s) 33, 147, 249, 596
Hsp92I GRCGYC 3 cut(s) 33, 238, 249
Hsp92II CATG 4 cut(s) 64, 112, 414, 556
HspAI GCGC 1 cut(s) 238
KasI GGCGCC 1 cut(s) 237
KspI CCGCGG 1 cut(s) 142
Kzo9I GATC 3 cut(s) 508, 668, 682
LmnI GCTCC 3 cut(s) 593, 622, 767
Lsp1109I GCAGC 1 cut(s) 223
LweI GCATC 1 cut(s) 606
MaeI CTAG 1 cut(s) 381
MaeII ACGT 4 cut(s) 33, 147, 249, 596
MaeIII GTNAC 1 cut(s) 761
MalI GATC 3 cut(s) 510, 670, 684
MbiI CCGCTC 1 cut(s) 625
MboI GATC 3 cut(s) 508, 668, 682
MboII GAAGA 3 cut(s) 112, 347, 447
MhlI GDGCHC 2 cut(s) 447, 786
MluCI AATT 3 cut(s) 176, 716, 817
Mly113I GGCGCC 1 cut(s) 238
MlyI GAGTC 3 cut(s) 264, 305, 769
MmeI TCCRAC 2 cut(s) 339, 615
MseI TTAA 1 cut(s) 720
MslI CAYNNNNRTG 1 cut(s) 49
MspA1I CMGCKG 1 cut(s) 141
MspI CCGG 1 cut(s) 521
MspR9I CCNGG 3 cut(s) 20, 522, 601
Mva1269I GAATGC 1 cut(s) 750
MvaI CCWGG 2 cut(s) 20, 601
MvnI CGCG 1 cut(s) 141
MwoI GCNNNNNNNGC 4 cut(s) 141, 208, 457, 625
NarI GGCGCC 1 cut(s) 238
NciI CCSGG 1 cut(s) 522
NcoI CCATGG 1 cut(s) 410
NdeI CATATG 1 cut(s) 50
NdeII GATC 3 cut(s) 508, 668, 682
NlaIII CATG 4 cut(s) 64, 112, 414, 556
NlaIV GGNNCC 6 cut(s) 157, 239, 535, 589, 605, 805
NmuCI GTSAC 1 cut(s) 761
PagI TCATGA 1 cut(s) 108
PcsI WCGNNNNNNNCGW 1 cut(s) 284
PctI GAATGC 1 cut(s) 750
PfeI GAWTC 4 cut(s) 77, 360, 630, 727
PkrI GCNGC 4 cut(s) 143, 213, 242, 627
PleI GAGTC 3 cut(s) 263, 305, 768
PluTI GGCGCC 1 cut(s) 241
PpsI GAGTC 3 cut(s) 263, 305, 768
PpuMI RGGWCCY 2 cut(s) 604, 803
Psp124BI GAGCTC 1 cut(s) 447
Psp5II RGGWCCY 2 cut(s) 604, 803
Psp6I CCWGG 2 cut(s) 18, 599
PspFI CCCAGC 1 cut(s) 460
PspGI CCWGG 2 cut(s) 18, 599
PspN4I GGNNCC 6 cut(s) 157, 239, 535, 589, 605, 805
PspPI GGNCC 4 cut(s) 155, 485, 604, 803
PspPPI RGGWCCY 2 cut(s) 604, 803
PstNI CAGNNNCTG 2 cut(s) 619, 711
RsaI GTAC 1 cut(s) 689
RsaNI GTAC 1 cut(s) 688
RseI CAYNNNNRTG 1 cut(s) 49
SacI GAGCTC 1 cut(s) 447
SacII CCGCGG 1 cut(s) 142
SalI GTCGAC 1 cut(s) 565
SaqAI TTAA 1 cut(s) 720
SatI GCNGC 4 cut(s) 142, 212, 241, 626
Sau3AI GATC 3 cut(s) 508, 668, 682
Sau96I GGNCC 4 cut(s) 155, 485, 604, 803
SchI GAGTC 3 cut(s) 264, 305, 769
ScrFI CCNGG 3 cut(s) 20, 522, 601
SduI GDGCHC 2 cut(s) 447, 786
SfaNI GCATC 1 cut(s) 606
SfoI GGCGCC 1 cut(s) 239
Sfr303I CCGCGG 1 cut(s) 142
SgrBI CCGCGG 1 cut(s) 142
SgrDI CGTCGACG 1 cut(s) 565
SinI GGWCC 2 cut(s) 604, 803
SmiMI CAYNNNNRTG 1 cut(s) 49
Sse9I AATT 3 cut(s) 176, 716, 817
SsiI CCGC 8 cut(s) 70, 139, 141, 241, 299, 625, 678, 748
SspDI GGCGCC 1 cut(s) 237
SspMI CTAG 1 cut(s) 381
SstI GAGCTC 1 cut(s) 447
StyD4I CCNGG 3 cut(s) 18, 520, 599
StyI CCWWGG 1 cut(s) 410
TaaI ACNGT 1 cut(s) 781
TaiI ACGT 4 cut(s) 36, 150, 252, 599
TaqI TCGA 5 cut(s) 75, 309, 358, 424, 566
TasI AATT 3 cut(s) 176, 716, 817
TauI GCSGC 3 cut(s) 144, 243, 628
TfiI GAWTC 4 cut(s) 77, 360, 630, 727
Tru1I TTAA 1 cut(s) 720
Tru9I TTAA 1 cut(s) 720
TseFI GTSAC 1 cut(s) 761
TseI GCWGC 1 cut(s) 211
Tsp45I GTSAC 1 cut(s) 761
TspDTI ATGAA 2 cut(s) 33, 82
TspGWI ACGGA 3 cut(s) 526, 675, 780
VpaK11BI GGWCC 2 cut(s) 604, 803
XapI RAATTY 3 cut(s) 176, 716, 817
XmiI GTMKAC 1 cut(s) 566
XspI CTAG 1 cut(s) 381
ZraI GACGTC 2 cut(s) 34, 250
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.