RLG00000022003

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0001758
Physical Location & Seq
Forward (+)
79876903 .. 79881458
4556 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022003

Sequence Viewer

Length: 438 bp
ATGATGCAGATCCCAGTCGCCCCGAAAGCCGACGGCGTCGACAGCAACACCTCGGCTCTGGCTCGATCTAGCAACCCCCTTCCTCATAACCACCCTCAGTTGAGCATGGACTCAACCGGAGCCCTGGAAAAGCTTGGTCGAGATCGCGACGCTGTTGCAGAAGAGACCACAGCCTCTCCAAGCCCACGCCGCCCTTCACCCGCCTTTTGCGGCTGGGCTTCTTGGCTCCGATCTGCAACAGGGCGCTTCGGCGTTGCAAGCGAGATCGGCGTTTGCTTTGGTGGAATTGTGTTTGACAAGGAGGAGATTGGCGTCACAGTTTCGGGACTAGAGGAGGAGCGAGGTCGAAGAATTCTTGCTAGAGATCGGCAGTCGAATCGCCGGTTGAGGCCTCGGGTTATTGCTTGCAGAACCAGTCTAGTGAAACAAGTGCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

146

Amino Acids

15.75

Weight (kDa)

9.73

Isoelectric Point (pI)

71.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 39
AccII CGCG 1 cut(s) 147
AciI CCGC 3 cut(s) 190, 201, 210
AclWI GGATC 1 cut(s) 4
AcsI RAATTY 1 cut(s) 351
AcyI GRCGYC 2 cut(s) 36, 312
AjnI CCWGG 1 cut(s) 123
AluBI AGCT 1 cut(s) 133
AluI AGCT 1 cut(s) 133
Alw26I GTCTC 1 cut(s) 158
AlwI GGATC 1 cut(s) 4
Ama87I CYCGRG 1 cut(s) 393
AoxI GGCC 1 cut(s) 389
ApoI RAATTY 1 cut(s) 351
AspLEI GCGC 1 cut(s) 246
AsuHPI GGTGA 1 cut(s) 189
AvaI CYCGRG 1 cut(s) 393
BanII GRGCYC 1 cut(s) 124
BceAI ACGGC 1 cut(s) 49
BcgI CGANNNNNNTGC 4 cut(s) 137, 171, 359, 393
BciT130I CCWGG 1 cut(s) 125
BcoDI GTCTC 1 cut(s) 158
BfaI CTAG 4 cut(s) 69, 329, 360, 419
BfoI RGCGCY 1 cut(s) 247
BisI GCNGC 2 cut(s) 190, 211
BlsI GCNGC 2 cut(s) 191, 212
Bme1390I CCNGG 1 cut(s) 125
BmeT110I CYCGRG 1 cut(s) 393
BmiI GGNNCC 2 cut(s) 121, 227
BmrFI CCNGG 1 cut(s) 125
BmrI ACTGGG 1 cut(s) 8
BmuI ACTGGG 1 cut(s) 8
BsaBI GATNNNNATC 1 cut(s) 8
BsaHI GRCGYC 2 cut(s) 36, 312
BsaI GGTCTC 1 cut(s) 158
BsaJI CCNNGG 3 cut(s) 51, 123, 392
BsaWI WCCGGW 1 cut(s) 116
BsaXI ACNNNNNCTCC 2 cut(s) 160, 190
Bse118I RCCGGY 1 cut(s) 381
Bse1I ACTGG 2 cut(s) 14, 414
Bse8I GATNNNNATC 1 cut(s) 8
BseBI CCWGG 1 cut(s) 125
BseDI CCNNGG 3 cut(s) 51, 123, 392
BseJI GATNNNNATC 1 cut(s) 8
BseMII CTCAG 1 cut(s) 110
BseNI ACTGG 2 cut(s) 14, 414
BseRI GAGGAG 3 cut(s) 317, 347, 350
BseYI CCCAGC 1 cut(s) 213
Bsh1236I CGCG 1 cut(s) 147
BshFI GGCC 1 cut(s) 391
BsiHKCI CYCGRG 1 cut(s) 393
BsiSI CCGG 2 cut(s) 117, 382
BslFI GGGAC 1 cut(s) 339
BsmAI GTCTC 1 cut(s) 158
BsmFI GGGAC 1 cut(s) 339
BsnI GGCC 1 cut(s) 391
Bso31I GGTCTC 1 cut(s) 158
BsoBI CYCGRG 1 cut(s) 393
Bsp1286I GDGCHC 1 cut(s) 124
Bsp143I GATC 6 cut(s) 9, 65, 142, 230, 264, 364
Bsp68I TCGCGA 1 cut(s) 147
BspACI CCGC 3 cut(s) 190, 201, 210
BspANI GGCC 1 cut(s) 391
BspCNI CTCAG 1 cut(s) 109
BspFNI CGCG 1 cut(s) 147
BspLI GGNNCC 2 cut(s) 121, 227
BspPI GGATC 1 cut(s) 4
BspTNI GGTCTC 1 cut(s) 158
BsrFI RCCGGY 1 cut(s) 381
BsrI ACTGG 2 cut(s) 14, 414
BssAI RCCGGY 1 cut(s) 381
BssECI CCNNGG 3 cut(s) 51, 123, 392
BssMI GATC 6 cut(s) 9, 65, 142, 230, 264, 364
BssNI GRCGYC 2 cut(s) 36, 312
Bst2UI CCWGG 1 cut(s) 125
Bst4CI ACNGT 1 cut(s) 319
Bst6I CTCTTC 1 cut(s) 156
BstACI GRCGYC 2 cut(s) 36, 312
BstC8I GCNNGC 2 cut(s) 259, 406
BstDEI CTNAG 1 cut(s) 96
BstFNI CGCG 1 cut(s) 147
BstH2I RGCGCY 1 cut(s) 247
BstHHI GCGC 1 cut(s) 246
BstKTI GATC 6 cut(s) 12, 68, 145, 233, 267, 367
BstMAI GTCTC 1 cut(s) 158
BstMBI GATC 6 cut(s) 9, 65, 142, 230, 264, 364
BstMWI GCNNNNNNNGC 5 cut(s) 26, 42, 189, 258, 267
BstNI CCWGG 1 cut(s) 125
BstSCI CCNGG 1 cut(s) 123
BstUI CGCG 1 cut(s) 147
BstX2I RGATCY 1 cut(s) 9
BstYI RGATCY 1 cut(s) 9
BsuRI GGCC 1 cut(s) 391
BtuMI TCGCGA 1 cut(s) 147
Cac8I GCNNGC 2 cut(s) 259, 406
CfoI GCGC 1 cut(s) 246
Cfr10I RCCGGY 1 cut(s) 381
CseI GACGC 3 cut(s) 25, 158, 301
CviAII CATG 1 cut(s) 106
DdeI CTNAG 1 cut(s) 96
DpnI GATC 6 cut(s) 11, 67, 144, 232, 266, 366
DpnII GATC 6 cut(s) 9, 65, 142, 230, 264, 364
Eam1104I CTCTTC 1 cut(s) 156
EarI CTCTTC 1 cut(s) 156
Eco147I AGGCCT 1 cut(s) 391
Eco24I GRGCYC 1 cut(s) 124
Eco31I GGTCTC 1 cut(s) 158
Eco88I CYCGRG 1 cut(s) 393
EcoRI GAATTC 1 cut(s) 351
EcoRII CCWGG 1 cut(s) 123
EcoT38I GRGCYC 1 cut(s) 124
FaeI CATG 1 cut(s) 109
FaiI YATR 2 cut(s) 87, 107
FaqI GGGAC 1 cut(s) 339
FatI CATG 1 cut(s) 105
FauI CCCGC 1 cut(s) 208
FblI GTMKAC 1 cut(s) 39
Fnu4HI GCNGC 2 cut(s) 190, 211
FriOI GRGCYC 1 cut(s) 124
Fsp4HI GCNGC 2 cut(s) 190, 211
FspBI CTAG 4 cut(s) 69, 329, 360, 419
GlaI GCGC 1 cut(s) 245
GluI GCNGC 2 cut(s) 190, 211
GsaI CCCAGC 1 cut(s) 217
HaeII RGCGCY 1 cut(s) 247
HaeIII GGCC 1 cut(s) 391
HapII CCGG 2 cut(s) 117, 382
HgaI GACGC 3 cut(s) 25, 158, 301
HhaI GCGC 1 cut(s) 246
Hin1I GRCGYC 2 cut(s) 36, 312
Hin1II CATG 1 cut(s) 109
Hin6I GCGC 1 cut(s) 244
HinP1I GCGC 1 cut(s) 244
HincII GTYRAC 1 cut(s) 40
HindII GTYRAC 1 cut(s) 40
HindIII AAGCTT 1 cut(s) 131
HinfI GANTC 2 cut(s) 110, 376
HpaII CCGG 2 cut(s) 117, 382
HphI GGTGA 1 cut(s) 189
Hpy166II GTNNAC 1 cut(s) 40
Hpy188I TCNGA 1 cut(s) 230
Hpy188III TCNNGA 3 cut(s) 140, 146, 324
Hpy8I GTNNAC 1 cut(s) 40
Hpy99I CGWCG 3 cut(s) 35, 41, 152
HpyAV CCTTC 2 cut(s) 89, 204
HpyCH4III ACNGT 1 cut(s) 319
HpyCH4V TGCA 6 cut(s) 7, 158, 236, 257, 408, 433
HpyF10VI GCNNNNNNNGC 5 cut(s) 26, 42, 189, 258, 267
HpyF3I CTNAG 1 cut(s) 96
Hsp92I GRCGYC 2 cut(s) 36, 312
Hsp92II CATG 1 cut(s) 109
HspAI GCGC 1 cut(s) 244
Kzo9I GATC 6 cut(s) 9, 65, 142, 230, 264, 364
LmnI GCTCC 3 cut(s) 119, 231, 337
LpnPI CCDG 9 cut(s) 27, 44, 110, 130, 137, 199, 225, 395, 427
MaeI CTAG 4 cut(s) 69, 329, 360, 419
MaeIII GTNAC 1 cut(s) 313
MalI GATC 6 cut(s) 11, 67, 144, 232, 266, 366
MboI GATC 6 cut(s) 9, 65, 142, 230, 264, 364
MboII GAAGA 2 cut(s) 173, 360
MflI RGATCY 1 cut(s) 9
MhlI GDGCHC 1 cut(s) 124
MluCI AATT 2 cut(s) 285, 351
MlyI GAGTC 1 cut(s) 104
MspI CCGG 2 cut(s) 117, 382
MspR9I CCNGG 1 cut(s) 125
MvaI CCWGG 1 cut(s) 125
MvnI CGCG 1 cut(s) 147
MwoI GCNNNNNNNGC 5 cut(s) 26, 42, 189, 258, 267
NdeII GATC 6 cut(s) 9, 65, 142, 230, 264, 364
NlaIII CATG 1 cut(s) 109
NlaIV GGNNCC 2 cut(s) 121, 227
NmeAIII GCCGAG 1 cut(s) 32
NmuCI GTSAC 1 cut(s) 313
NruI TCGCGA 1 cut(s) 147
PceI AGGCCT 1 cut(s) 391
PfeI GAWTC 1 cut(s) 376
PflFI GACNNNGTC 1 cut(s) 35
PkrI GCNGC 2 cut(s) 191, 212
PleI GAGTC 1 cut(s) 104
PpsI GAGTC 1 cut(s) 104
Psp6I CCWGG 1 cut(s) 123
PspFI CCCAGC 1 cut(s) 213
PspGI CCWGG 1 cut(s) 123
PspN4I GGNNCC 2 cut(s) 121, 227
PsuI RGATCY 1 cut(s) 9
PsyI GACNNNGTC 1 cut(s) 35
RruI TCGCGA 1 cut(s) 147
SalI GTCGAC 1 cut(s) 38
SatI GCNGC 2 cut(s) 190, 211
Sau3AI GATC 6 cut(s) 9, 65, 142, 230, 264, 364
SchI GAGTC 1 cut(s) 104
ScrFI CCNGG 1 cut(s) 125
SduI GDGCHC 1 cut(s) 124
SetI ASST 3 cut(s) 53, 135, 346
Sse9I AATT 2 cut(s) 285, 351
SseBI AGGCCT 1 cut(s) 391
SsiI CCGC 3 cut(s) 190, 201, 210
SspMI CTAG 4 cut(s) 69, 329, 360, 419
StuI AGGCCT 1 cut(s) 391
StyD4I CCNGG 1 cut(s) 123
TaaI ACNGT 1 cut(s) 319
TaqI TCGA 5 cut(s) 39, 64, 139, 346, 374
TasI AATT 2 cut(s) 285, 351
TauI GCSGC 2 cut(s) 192, 213
TfiI GAWTC 1 cut(s) 376
TseFI GTSAC 1 cut(s) 313
Tsp45I GTSAC 1 cut(s) 313
Tth111I GACNNNGTC 1 cut(s) 35
XapI RAATTY 1 cut(s) 351
XmiI GTMKAC 1 cut(s) 39
XspI CTAG 4 cut(s) 69, 329, 360, 419
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.