Rroxscaffold_1G00072860

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0001758
Physical Location & Seq
Forward (+)
93842721 .. 93843502
782 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00072860.1

Sequence Viewer

Length: 567 bp
ATGAGTACTATAAGGAACATTGTCCGCAACCTCATGCGCCATAAGGGTCTTGGTAAGCTTTCGATTTTTGTTTCAGGGTTCGTGAGAAAGATTATCAGCAATTTTCTGGGGCTGATCCGTTTGTTCCGAAGGAAAAAAGAAACGATCGCGGTGTGTGCTAGTTCGACTGATCAGGATCTGAACCTAGGGCTCCTTAGTGAGACTGAACACAAGAAAGAACATTATGAAGCATCTAGCTCCTTTACTCCGATGGAGCTGCAAAGAGGTACCAAGGCAGAAGAACCCGAAGCTGCTGGAAACCCTTTGATTCAACCTGTAGTCAAAAGAGAACACATTTTCTGTCGATACTGTTTTATCTTTGGAGATCATAAAACTTCAGTTTGCCCGAAGAAACATTACATATGTACAGACTGTGGTACCCTATTTCCCTGCGAGAAGAAGGACGTCCATGGCGAAGAACATAAAGTATTAAATTATTGCTGTGGTTGTAAAAAATGGGGCCACTTGTTAGAAATGTGCCCTGACCTTGATGATTCGGATGTAAACTATGACCCTCCAGACTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

188

Amino Acids

21.52

Weight (kDa)

7.99

Isoelectric Point (pI)

45.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 447
Acc65I GGTACC 2 cut(s) 266, 416
AccB1I GGYRCC 2 cut(s) 266, 416
AccII CGCG 1 cut(s) 149
AciI CCGC 2 cut(s) 25, 149
AclWI GGATC 2 cut(s) 109, 183
AcuI CTGAAG 1 cut(s) 360
AcyI GRCGYC 1 cut(s) 444
AfaI GTAC 4 cut(s) 7, 268, 406, 418
AgsI TTSAA 1 cut(s) 311
AluBI AGCT 4 cut(s) 58, 237, 256, 290
AluI AGCT 4 cut(s) 58, 237, 256, 290
Alw26I GTCTC 1 cut(s) 194
AlwI GGATC 2 cut(s) 109, 183
AlwNI CAGNNNCTG 1 cut(s) 178
AoxI GGCC 1 cut(s) 499
ApeKI GCWGC 2 cut(s) 256, 290
Asp718I GGTACC 2 cut(s) 266, 416
AspA2I CCTAGG 1 cut(s) 184
AspLEI GCGC 1 cut(s) 39
AspS9I GGNCC 1 cut(s) 499
AvrII CCTAGG 1 cut(s) 184
BaeGI GKGCMC 1 cut(s) 521
BaeI ACNNNNGTAYC 2 cut(s) 400, 433
BanI GGYRCC 2 cut(s) 266, 416
BanII GRGCYC 1 cut(s) 192
BarI GAAGNNNNNNTAC 2 cut(s) 380, 412
BbvI GCAGC 2 cut(s) 243, 277
BccI CCATC 1 cut(s) 244
BcgI CGANNNNNNTGC 2 cut(s) 238, 272
BclI TGATCA 1 cut(s) 169
BcoDI GTCTC 1 cut(s) 194
BfaI CTAG 3 cut(s) 159, 185, 234
BfmI CTRYAG 1 cut(s) 315
BisI GCNGC 2 cut(s) 257, 291
BlnI CCTAGG 1 cut(s) 184
BlsI GCNGC 2 cut(s) 258, 292
BmcAI AGTACT 1 cut(s) 7
BmgT120I GGNCC 1 cut(s) 499
BmiI GGNNCC 4 cut(s) 191, 268, 418, 500
BmsI GCATC 1 cut(s) 239
BpmI CTGGAG 1 cut(s) 540
BsaBI GATNNNNATC 1 cut(s) 174
BsaHI GRCGYC 1 cut(s) 444
BsaJI CCNNGG 3 cut(s) 184, 270, 448
Bse1I ACTGG 1 cut(s) 566
Bse8I GATNNNNATC 1 cut(s) 174
BseDI CCNNGG 3 cut(s) 184, 270, 448
BseGI GGATG 1 cut(s) 544
BseJI GATNNNNATC 1 cut(s) 174
BseNI ACTGG 1 cut(s) 566
BseSI GKGCMC 1 cut(s) 521
BseXI GCAGC 2 cut(s) 243, 277
Bsh1236I CGCG 1 cut(s) 149
Bsh1285I CGRYCG 1 cut(s) 147
BshFI GGCC 1 cut(s) 501
BshNI GGYRCC 2 cut(s) 266, 416
BsiEI CGRYCG 1 cut(s) 147
BsmAI GTCTC 1 cut(s) 194
BsnI GGCC 1 cut(s) 501
Bsp1286I GDGCHC 2 cut(s) 192, 521
Bsp1407I TGTACA 1 cut(s) 404
Bsp143I GATC 5 cut(s) 114, 144, 169, 175, 364
Bsp19I CCATGG 1 cut(s) 448
BspACI CCGC 2 cut(s) 25, 149
BspANI GGCC 1 cut(s) 501
BspFNI CGCG 1 cut(s) 149
BspLI GGNNCC 4 cut(s) 191, 268, 418, 500
BspPI GGATC 2 cut(s) 109, 183
BspT107I GGYRCC 2 cut(s) 266, 416
BsrGI TGTACA 1 cut(s) 404
BsrI ACTGG 1 cut(s) 566
BssECI CCNNGG 3 cut(s) 184, 270, 448
BssMI GATC 5 cut(s) 114, 144, 169, 175, 364
BssNI GRCGYC 1 cut(s) 444
BssT1I CCWWGG 3 cut(s) 184, 270, 448
Bst4CI ACNGT 2 cut(s) 350, 413
BstACI GRCGYC 1 cut(s) 444
BstAUI TGTACA 1 cut(s) 404
BstDEI CTNAG 1 cut(s) 194
BstDSI CCRYGG 1 cut(s) 448
BstF5I GGATG 1 cut(s) 544
BstFNI CGCG 1 cut(s) 149
BstHHI GCGC 1 cut(s) 39
BstKTI GATC 5 cut(s) 117, 147, 172, 178, 367
BstMAI GTCTC 1 cut(s) 194
BstMBI GATC 5 cut(s) 114, 144, 169, 175, 364
BstMCI CGRYCG 1 cut(s) 147
BstMWI GCNNNNNNNGC 1 cut(s) 155
BstSFI CTRYAG 1 cut(s) 315
BstSLI GKGCMC 1 cut(s) 521
BstUI CGCG 1 cut(s) 149
BstV1I GCAGC 2 cut(s) 243, 277
BstX2I RGATCY 1 cut(s) 175
BstYI RGATCY 1 cut(s) 175
BsuRI GGCC 1 cut(s) 501
BtgI CCRYGG 1 cut(s) 448
BtsCI GGATG 1 cut(s) 544
CaiI CAGNNNCTG 1 cut(s) 178
CfoI GCGC 1 cut(s) 39
Cfr13I GGNCC 1 cut(s) 499
Csp6I GTAC 4 cut(s) 6, 267, 405, 417
CviAII CATG 2 cut(s) 34, 449
CviJI RGCY 7 cut(s) 58, 112, 190, 237, 256, 290, 501
CviKI_1 RGCY 7 cut(s) 58, 112, 190, 237, 256, 290, 501
CviQI GTAC 4 cut(s) 6, 267, 405, 417
DdeI CTNAG 1 cut(s) 194
DpnI GATC 5 cut(s) 116, 146, 171, 177, 366
DpnII GATC 5 cut(s) 114, 144, 169, 175, 364
Eco130I CCWWGG 3 cut(s) 184, 270, 448
Eco24I GRGCYC 1 cut(s) 192
Eco57I CTGAAG 1 cut(s) 360
EcoT14I CCWWGG 3 cut(s) 184, 270, 448
EcoT38I GRGCYC 1 cut(s) 192
ErhI CCWWGG 3 cut(s) 184, 270, 448
FaeI CATG 2 cut(s) 37, 452
FatI CATG 2 cut(s) 33, 448
FauNDI CATATG 1 cut(s) 401
FbaI TGATCA 1 cut(s) 169
Fnu4HI GCNGC 2 cut(s) 257, 291
FokI GGATG 1 cut(s) 551
FriOI GRGCYC 1 cut(s) 192
Fsp4HI GCNGC 2 cut(s) 257, 291
FspBI CTAG 3 cut(s) 159, 185, 234
GlaI GCGC 1 cut(s) 38
GluI GCNGC 2 cut(s) 257, 291
GsuI CTGGAG 1 cut(s) 540
HaeIII GGCC 1 cut(s) 501
HhaI GCGC 1 cut(s) 39
Hin1I GRCGYC 1 cut(s) 444
Hin1II CATG 2 cut(s) 37, 452
Hin6I GCGC 1 cut(s) 37
HinP1I GCGC 1 cut(s) 37
HindIII AAGCTT 1 cut(s) 56
HinfI GANTC 2 cut(s) 307, 533
Hpy166II GTNNAC 1 cut(s) 544
Hpy188I TCNGA 4 cut(s) 128, 180, 249, 538
Hpy188III TCNNGA 3 cut(s) 82, 173, 557
Hpy8I GTNNAC 1 cut(s) 544
HpyAV CCTTC 2 cut(s) 123, 433
HpyCH4III ACNGT 2 cut(s) 350, 413
HpyCH4IV ACGT 1 cut(s) 444
HpyCH4V TGCA 1 cut(s) 259
HpyF10VI GCNNNNNNNGC 1 cut(s) 155
HpyF3I CTNAG 1 cut(s) 194
HpySE526I ACGT 1 cut(s) 444
Hsp92I GRCGYC 1 cut(s) 444
Hsp92II CATG 2 cut(s) 37, 452
HspAI GCGC 1 cut(s) 37
KpnI GGTACC 2 cut(s) 270, 420
Ksp22I TGATCA 1 cut(s) 169
Kzo9I GATC 5 cut(s) 114, 144, 169, 175, 364
LmnI GCTCC 3 cut(s) 195, 242, 253
LpnPI CCDG 8 cut(s) 60, 92, 158, 279, 327, 442, 534, 547
Lsp1109I GCAGC 2 cut(s) 243, 277
LweI GCATC 1 cut(s) 239
MaeI CTAG 3 cut(s) 159, 185, 234
MaeII ACGT 1 cut(s) 444
MalI GATC 5 cut(s) 116, 146, 171, 177, 366
MboI GATC 5 cut(s) 114, 144, 169, 175, 364
MboII GAAGA 4 cut(s) 290, 400, 448, 467
MflI RGATCY 1 cut(s) 175
MhlI GDGCHC 2 cut(s) 192, 521
MluCI AATT 2 cut(s) 100, 472
MnlI CCTC 3 cut(s) 41, 257, 564
MseI TTAA 1 cut(s) 470
MvnI CGCG 1 cut(s) 149
MwoI GCNNNNNNNGC 1 cut(s) 155
NcoI CCATGG 1 cut(s) 448
NdeI CATATG 1 cut(s) 401
NdeII GATC 5 cut(s) 114, 144, 169, 175, 364
NlaIII CATG 2 cut(s) 37, 452
NlaIV GGNNCC 4 cut(s) 191, 268, 418, 500
PcsI WCGNNNNNNNCGW 1 cut(s) 450
PfeI GAWTC 2 cut(s) 307, 533
PkrI GCNGC 2 cut(s) 258, 292
Ple19I CGATCG 1 cut(s) 147
PspN4I GGNNCC 4 cut(s) 191, 268, 418, 500
PspPI GGNCC 1 cut(s) 499
PstNI CAGNNNCTG 1 cut(s) 178
PsuI RGATCY 1 cut(s) 175
PvuI CGATCG 1 cut(s) 147
RsaI GTAC 4 cut(s) 7, 268, 406, 418
RsaNI GTAC 4 cut(s) 6, 267, 405, 417
SaqAI TTAA 1 cut(s) 470
SatI GCNGC 2 cut(s) 257, 291
Sau3AI GATC 5 cut(s) 114, 144, 169, 175, 364
Sau96I GGNCC 1 cut(s) 499
ScaI AGTACT 1 cut(s) 7
SduI GDGCHC 2 cut(s) 192, 521
SfaNI GCATC 1 cut(s) 239
SfcI CTRYAG 1 cut(s) 315
Sse9I AATT 2 cut(s) 100, 472
SsiI CCGC 2 cut(s) 25, 149
SspMI CTAG 3 cut(s) 159, 185, 234
StyI CCWWGG 3 cut(s) 184, 270, 448
TaaI ACNGT 2 cut(s) 350, 413
TaiI ACGT 1 cut(s) 447
TaqI TCGA 3 cut(s) 62, 164, 343
TasI AATT 2 cut(s) 100, 472
TatI WGTACW 2 cut(s) 5, 404
TfiI GAWTC 2 cut(s) 307, 533
Tru1I TTAA 1 cut(s) 470
Tru9I TTAA 1 cut(s) 470
TseI GCWGC 2 cut(s) 256, 290
TspDTI ATGAA 1 cut(s) 240
TspGWI ACGGA 1 cut(s) 107
XcmI CCANNNNNNNNNTGG 1 cut(s) 47
XmaJI CCTAGG 1 cut(s) 184
XspI CTAG 3 cut(s) 159, 185, 234
ZraI GACGTC 1 cut(s) 445
ZrmI AGTACT 1 cut(s) 7
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.