Rorug04G0401600

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0001758
Physical Location & Seq
Reverse (-)
56616968 .. 56618790
1823 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0401600.1

Sequence Viewer

Length: 951 bp
ATGCAGACCCTTGTGAAATTAGCAACGAAGCCTACTCTGCGTAACACTCTGAGCAAAACCCAATTCACCTCCACCGTCAATTTCAAATCTTTCTCCACCAAACCCTCCGCGCCGCCCAATCGGAACCCTAAGAAAGCCGACGACGAGTGGAACGACGCGTGGGAGACCGCGTGGCTCCCGCCGGACCCGTCCTCCGAGAACGCCAGAGCTCCATGGGAGACCGACGAGAATCAGACGGCGGCGGCGGAGGAGGTCGTGATTCCCTCCGACTCCGACTCCGACGCCGACGCCGAGACGAAGGCGTTTGTGGAGGACATGAATGCGAATTGGGACGAGAGACGGAAACCCAAGGAGGAGAAGAAACAGCAGCAGAGTGAGAGTGGGAAGCTTTATAGCTTGGAGAATATAAAGAAGGACTATAGGTTGAAGAAGCAGAGGATTCATGCCGGGCTTTGGATGAAGGAGATTGAGAAGCAACAAGAGGCTAAATTAGCTGATTCCGGTTCCATAGGTGGTGGTGACGATATCGAACGCTTGCTTGATAGCTGCTCCGATATTTTCGATTCTACGAACAATGATTTGGAAAATGCGAAAGTGCCGAGCGCTGATGATTTCAAGAACAAGCCTGATGGTTGGGAAACGACAGCCAAGGCTGAAGAGGGAAGTGTATGGGAGATGACACAGAGGGAAGAAGATATCCTTCTCCAAGAGTTTGAGCGTCGAATTGCGTATAATAAGTTTCAGATTGCTAGTTTTATCAAGACTCACATATTTAGTCGAAGGAGGCCAATCGATGGGTGGAAATACATGATAGAGGAGCTGGGGCCAAATGCTAGGAAGGGAAAAGGTGCTGTTTCAAGGTTACCAAATTTATCCGATGCATCAACCCAACCCTTCAAGGAGGAGAGGACTCCAATTAGCACCAGTCTTACCTCCTACAAGGATAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

316

Amino Acids

36.03

Weight (kDa)

5.21

Isoelectric Point (pI)

53.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 794
AccII CGCG 3 cut(s) 110, 158, 170
AciI CCGC 7 cut(s) 108, 113, 168, 179, 239, 242, 245
AcsI RAATTY 1 cut(s) 868
AcuI CTGAAG 1 cut(s) 675
AcyI GRCGYC 2 cut(s) 282, 288
AfeI AGCGCT 1 cut(s) 604
AfiI CCNNNNNNNGG 2 cut(s) 453, 794
AflIII ACRYGT 1 cut(s) 156
AgsI TTSAA 5 cut(s) 85, 427, 616, 858, 898
AjuI GAANNNNNNNTTGG 2 cut(s) 563, 595
AluBI AGCT 6 cut(s) 209, 388, 396, 494, 546, 820
AluI AGCT 6 cut(s) 209, 388, 396, 494, 546, 820
Alw21I GWGCWC 1 cut(s) 211
Alw26I GTCTC 4 cut(s) 158, 212, 287, 331
Aor51HI AGCGCT 1 cut(s) 604
AoxI GGCC 2 cut(s) 785, 824
ApeKI GCWGC 2 cut(s) 367, 546
ApoI RAATTY 1 cut(s) 868
AspLEI GCGC 2 cut(s) 112, 605
AspS9I GGNCC 2 cut(s) 184, 824
AsuC2I CCSGG 1 cut(s) 448
AsuHPI GGTGA 2 cut(s) 58, 530
AvaII GGWCC 1 cut(s) 184
BanII GRGCYC 1 cut(s) 211
Bbv12I GWGCWC 1 cut(s) 211
BbvI GCAGC 2 cut(s) 379, 533
BccI CCATC 2 cut(s) 623, 788
BceAI ACGGC 1 cut(s) 252
BcnI CCSGG 1 cut(s) 448
BcoDI GTCTC 4 cut(s) 158, 212, 287, 331
BfaI CTAG 2 cut(s) 750, 834
BfmI CTRYAG 1 cut(s) 418
BfoI RGCGCY 1 cut(s) 606
BisI GCNGC 5 cut(s) 113, 240, 243, 368, 547
BlsI GCNGC 5 cut(s) 114, 241, 244, 369, 548
Bme1390I CCNGG 1 cut(s) 448
Bme18I GGWCC 1 cut(s) 184
BmgT120I GGNCC 2 cut(s) 184, 824
BmiI GGNNCC 5 cut(s) 125, 176, 186, 505, 825
BmrFI CCNGG 1 cut(s) 448
BmsI GCATC 2 cut(s) 868, 890
BpuMI CCSGG 1 cut(s) 448
Bsa29I ATCGAT 1 cut(s) 792
BsaHI GRCGYC 2 cut(s) 282, 288
BsaI GGTCTC 2 cut(s) 158, 212
BsaJI CCNNGG 3 cut(s) 212, 348, 648
BsaWI WCCGGW 1 cut(s) 500
BsaXI ACNNNNNCTCC 6 cut(s) 155, 176, 185, 206, 260, 290
Bsc4I CCNNNNNNNGG 2 cut(s) 453, 794
Bse1I ACTGG 1 cut(s) 924
BseCI ATCGAT 1 cut(s) 792
BseDI CCNNGG 3 cut(s) 212, 348, 648
BseGI GGATG 1 cut(s) 462
BseLI CCNNNNNNNGG 2 cut(s) 453, 794
BseMII CTCAG 1 cut(s) 41
BseNI ACTGG 1 cut(s) 924
BseRI GAGGAG 4 cut(s) 263, 368, 830, 917
BseXI GCAGC 2 cut(s) 379, 533
BseYI CCCAGC 1 cut(s) 820
Bsh1236I CGCG 3 cut(s) 110, 158, 170
BshFI GGCC 2 cut(s) 787, 826
BshVI ATCGAT 1 cut(s) 792
BsiHKAI GWGCWC 1 cut(s) 211
BsiSI CCGG 3 cut(s) 182, 447, 501
BslFI GGGAC 1 cut(s) 344
BslI CCNNNNNNNGG 2 cut(s) 453, 794
BsmAI GTCTC 4 cut(s) 158, 212, 287, 331
BsmBI CGTCTC 2 cut(s) 287, 331
BsmFI GGGAC 1 cut(s) 344
BsmI GAATGC 1 cut(s) 325
BsnI GGCC 2 cut(s) 787, 826
Bso31I GGTCTC 2 cut(s) 158, 212
Bsp1286I GDGCHC 1 cut(s) 211
Bsp19I CCATGG 1 cut(s) 212
BspACI CCGC 7 cut(s) 108, 113, 168, 179, 239, 242, 245
BspANI GGCC 2 cut(s) 787, 826
BspCNI CTCAG 1 cut(s) 42
BspDI ATCGAT 1 cut(s) 792
BspFNI CGCG 3 cut(s) 110, 158, 170
BspLI GGNNCC 5 cut(s) 125, 176, 186, 505, 825
BspTNI GGTCTC 2 cut(s) 158, 212
BsrI ACTGG 1 cut(s) 924
BssECI CCNNGG 3 cut(s) 212, 348, 648
BssNI GRCGYC 2 cut(s) 282, 288
BssT1I CCWWGG 3 cut(s) 212, 348, 648
Bst4CI ACNGT 1 cut(s) 76
Bst6I CTCTTC 1 cut(s) 651
BstACI GRCGYC 2 cut(s) 282, 288
BstC8I GCNNGC 1 cut(s) 536
BstDEI CTNAG 2 cut(s) 50, 129
BstDSI CCRYGG 1 cut(s) 212
BstEII GGTNACC 1 cut(s) 861
BstF5I GGATG 1 cut(s) 462
BstFNI CGCG 3 cut(s) 110, 158, 170
BstH2I RGCGCY 1 cut(s) 606
BstHHI GCGC 2 cut(s) 112, 605
BstMAI GTCTC 4 cut(s) 158, 212, 287, 331
BstMWI GCNNNNNNNGC 2 cut(s) 37, 491
BstPI GGTNACC 1 cut(s) 861
BstSCI CCNGG 1 cut(s) 446
BstSFI CTRYAG 1 cut(s) 418
BstUI CGCG 3 cut(s) 110, 158, 170
BstV1I GCAGC 2 cut(s) 379, 533
Bsu15I ATCGAT 1 cut(s) 792
BsuRI GGCC 2 cut(s) 787, 826
BsuTUI ATCGAT 1 cut(s) 792
BtgI CCRYGG 1 cut(s) 212
BtsCI GGATG 1 cut(s) 462
Cac8I GCNNGC 1 cut(s) 536
CfoI GCGC 2 cut(s) 112, 605
Cfr13I GGNCC 2 cut(s) 184, 824
ClaI ATCGAT 1 cut(s) 792
CseI GACGC 4 cut(s) 164, 290, 296, 707
CviAII CATG 4 cut(s) 213, 316, 443, 808
DdeI CTNAG 2 cut(s) 50, 129
Eam1104I CTCTTC 1 cut(s) 651
EarI CTCTTC 1 cut(s) 651
EciI GGCGGA 1 cut(s) 260
Ecl136II GAGCTC 1 cut(s) 209
Eco130I CCWWGG 3 cut(s) 212, 348, 648
Eco24I GRGCYC 1 cut(s) 211
Eco31I GGTCTC 2 cut(s) 158, 212
Eco32I GATATC 2 cut(s) 526, 697
Eco47I GGWCC 1 cut(s) 184
Eco47III AGCGCT 1 cut(s) 604
Eco53kI GAGCTC 1 cut(s) 209
Eco57I CTGAAG 1 cut(s) 675
Eco91I GGTNACC 1 cut(s) 861
EcoICRI GAGCTC 1 cut(s) 209
EcoO65I GGTNACC 1 cut(s) 861
EcoRV GATATC 2 cut(s) 526, 697
EcoT14I CCWWGG 3 cut(s) 212, 348, 648
EcoT22I ATGCAT 1 cut(s) 883
EcoT38I GRGCYC 1 cut(s) 211
ErhI CCWWGG 3 cut(s) 212, 348, 648
Esp3I CGTCTC 2 cut(s) 287, 331
FaeI CATG 4 cut(s) 216, 319, 446, 811
FalI AAGNNNNNCTT 2 cut(s) 684, 716
FaqI GGGAC 1 cut(s) 344
FatI CATG 4 cut(s) 212, 315, 442, 807
FauI CCCGC 1 cut(s) 186
Fnu4HI GCNGC 5 cut(s) 113, 240, 243, 368, 547
FokI GGATG 1 cut(s) 469
FriOI GRGCYC 1 cut(s) 211
Fsp4HI GCNGC 5 cut(s) 113, 240, 243, 368, 547
FspBI CTAG 2 cut(s) 750, 834
GlaI GCGC 2 cut(s) 111, 604
GluI GCNGC 5 cut(s) 113, 240, 243, 368, 547
GsaI CCCAGC 1 cut(s) 824
HaeII RGCGCY 1 cut(s) 606
HaeIII GGCC 2 cut(s) 787, 826
HapII CCGG 3 cut(s) 182, 447, 501
HgaI GACGC 4 cut(s) 164, 290, 296, 707
HhaI GCGC 2 cut(s) 112, 605
Hin1I GRCGYC 2 cut(s) 282, 288
Hin1II CATG 4 cut(s) 216, 319, 446, 811
Hin6I GCGC 2 cut(s) 110, 603
HinP1I GCGC 2 cut(s) 110, 603
HindIII AAGCTT 1 cut(s) 386
HinfI GANTC 9 cut(s) 229, 259, 269, 275, 439, 497, 563, 763, 910
HpaII CCGG 3 cut(s) 182, 447, 501
HphI GGTGA 2 cut(s) 58, 530
Hpy188III TCNNGA 3 cut(s) 256, 616, 760
Hpy99I CGWCG 7 cut(s) 143, 146, 158, 227, 284, 290, 723
HpyAV CCTTC 7 cut(s) 292, 406, 454, 710, 774, 832, 904
HpyCH4III ACNGT 1 cut(s) 76
HpyCH4V TGCA 2 cut(s) 4, 881
HpyF10VI GCNNNNNNNGC 2 cut(s) 37, 491
HpyF3I CTNAG 2 cut(s) 50, 129
Hsp92I GRCGYC 2 cut(s) 282, 288
Hsp92II CATG 4 cut(s) 216, 319, 446, 811
HspAI GCGC 2 cut(s) 110, 603
LmnI GCTCC 4 cut(s) 180, 214, 554, 817
LpnPI CCDG 7 cut(s) 195, 217, 460, 514, 639, 806, 937
Lsp1109I GCAGC 2 cut(s) 379, 533
LweI GCATC 2 cut(s) 868, 890
MaeI CTAG 2 cut(s) 750, 834
MaeIII GTNAC 3 cut(s) 41, 518, 861
MboII GAAGA 5 cut(s) 370, 439, 668, 701, 704
MhlI GDGCHC 1 cut(s) 211
MluCI AATT 8 cut(s) 17, 62, 79, 325, 488, 723, 868, 915
MluI ACGCGT 1 cut(s) 156
MlyI GAGTC 4 cut(s) 263, 269, 757, 904
MmeI TCCRAC 3 cut(s) 291, 297, 303
Mph1103I ATGCAT 1 cut(s) 883
MspI CCGG 3 cut(s) 182, 447, 501
MspR9I CCNGG 1 cut(s) 448
Mva1269I GAATGC 1 cut(s) 325
MvnI CGCG 3 cut(s) 110, 158, 170
MwoI GCNNNNNNNGC 2 cut(s) 37, 491
NciI CCSGG 1 cut(s) 448
NcoI CCATGG 1 cut(s) 212
NlaIII CATG 4 cut(s) 216, 319, 446, 811
NlaIV GGNNCC 5 cut(s) 125, 176, 186, 505, 825
NmeAIII GCCGAG 2 cut(s) 316, 624
NmuCI GTSAC 1 cut(s) 518
NsiI ATGCAT 1 cut(s) 883
PcsI WCGNNNNNNNCGW 2 cut(s) 150, 288
PctI GAATGC 1 cut(s) 325
PfeI GAWTC 5 cut(s) 229, 259, 439, 497, 563
PflMI CCANNNNNTGG 1 cut(s) 794
PkrI GCNGC 5 cut(s) 114, 241, 244, 369, 548
PleI GAGTC 4 cut(s) 263, 269, 757, 904
PpsI GAGTC 4 cut(s) 263, 269, 757, 904
Psp124BI GAGCTC 1 cut(s) 211
PspEI GGTNACC 1 cut(s) 861
PspFI CCCAGC 1 cut(s) 820
PspN4I GGNNCC 5 cut(s) 125, 176, 186, 505, 825
PspPI GGNCC 2 cut(s) 184, 824
SacI GAGCTC 1 cut(s) 211
SatI GCNGC 5 cut(s) 113, 240, 243, 368, 547
Sau96I GGNCC 2 cut(s) 184, 824
SchI GAGTC 4 cut(s) 263, 269, 757, 904
ScrFI CCNGG 1 cut(s) 448
SduI GDGCHC 1 cut(s) 211
SfaNI GCATC 2 cut(s) 868, 890
SfcI CTRYAG 1 cut(s) 418
SinI GGWCC 1 cut(s) 184
Sse9I AATT 8 cut(s) 17, 62, 79, 325, 488, 723, 868, 915
SsiI CCGC 7 cut(s) 108, 113, 168, 179, 239, 242, 245
SspMI CTAG 2 cut(s) 750, 834
SstI GAGCTC 1 cut(s) 211
StyD4I CCNGG 1 cut(s) 446
StyI CCWWGG 3 cut(s) 212, 348, 648
TaaI ACNGT 1 cut(s) 76
TaqI TCGA 5 cut(s) 528, 561, 721, 778, 792
TaqII GACCGA 1 cut(s) 236
TasI AATT 8 cut(s) 17, 62, 79, 325, 488, 723, 868, 915
TauI GCSGC 3 cut(s) 115, 242, 245
TfiI GAWTC 5 cut(s) 229, 259, 439, 497, 563
TseFI GTSAC 1 cut(s) 518
TseI GCWGC 2 cut(s) 367, 546
Tsp45I GTSAC 1 cut(s) 518
TspDTI ATGAA 3 cut(s) 332, 431, 473
TspGWI ACGGA 1 cut(s) 355
Van91I CCANNNNNTGG 1 cut(s) 794
VpaK11BI GGWCC 1 cut(s) 184
XapI RAATTY 1 cut(s) 868
XcmI CCANNNNNNNNNTGG 1 cut(s) 795
XspI CTAG 2 cut(s) 750, 834
Zsp2I ATGCAT 1 cut(s) 883
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.