Rh5AG027300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0001758
Physical Location & Seq
Forward (+)
1874775 .. 1878031
3257 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG027300.1

Sequence Viewer

Length: 606 bp
ATGGTTCTCTTTTTTCACCCAGCCACAGTTCCCATGAGTATCAATCCAGTTGACGAGGACCTGAACCTTGGACTCCTTCATGAAGCTGACCAGAAGGAAGAACACGTTTGCCGCGACTTTTATTCATGTAAAAATCAGGAAGAGCATGGCAACAAAACAGAGGACTACATTTCTTGTCGACATTGTTATACGATTGGTGACCATAAAAGTGCAATGTGCCCAAATAAACCGAAGAAACGTGTAATGGTTTGCAGCGTTTGCCGCGACCTCTATCCCTGTAAAAATCAGGAAGAGCATGGCAACAATGGAGTTGAAGTAAACAATTTTTGTTATCGTTGTCGTACGAAGGGCCACTTGCCTCAAGAGTGCACAGAGGATTATGATCCAACTTTAACCTTGAACGAGAGGGATAATGCGATGGTGGAAATACGCCACGTTGTCGGAAACAGATATCACTCTTTGGCATCTGATCCCCATCGTTCCTTGAAAACAATTACAAATGGTGCCAACTCTGGCCCGATTGATTGTGTAGAAATACCACTGGACTATAACATGATAGATGATAATATTGGCAGTGTGGTGCTCCTGTTCTCTTTTCAATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

201

Amino Acids

22.94

Weight (kDa)

5.5

Isoelectric Point (pI)

43.07

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 503
AccI GTMKAC 1 cut(s) 178
AccII CGCG 2 cut(s) 114, 264
AciI CCGC 2 cut(s) 112, 262
AclWI GGATC 2 cut(s) 377, 464
AfaI GTAC 1 cut(s) 343
AflIII ACRYGT 2 cut(s) 103, 238
AgsI TTSAA 4 cut(s) 314, 400, 487, 599
AluBI AGCT 1 cut(s) 86
AluI AGCT 1 cut(s) 86
Alw21I GWGCWC 2 cut(s) 371, 585
Alw44I GTGCAC 1 cut(s) 367
AlwI GGATC 2 cut(s) 377, 464
AoxI GGCC 2 cut(s) 349, 514
ApaLI GTGCAC 1 cut(s) 367
ApeKI GCWGC 1 cut(s) 252
AspS9I GGNCC 3 cut(s) 58, 349, 515
AsuHPI GGTGA 2 cut(s) 8, 209
AvaII GGWCC 1 cut(s) 58
BaeGI GKGCMC 2 cut(s) 221, 371
BanI GGYRCC 1 cut(s) 503
Bbv12I GWGCWC 2 cut(s) 371, 585
BbvI GCAGC 1 cut(s) 264
BccI CCATC 2 cut(s) 412, 483
BisI GCNGC 3 cut(s) 112, 253, 262
BlsI GCNGC 3 cut(s) 113, 254, 263
Bme18I GGWCC 1 cut(s) 58
BmgT120I GGNCC 3 cut(s) 58, 349, 515
BmiI GGNNCC 1 cut(s) 505
BmsI GCATC 1 cut(s) 473
BpuEI CTTGAG 1 cut(s) 345
BsaBI GATNNNNATC 2 cut(s) 381, 474
BsaJI CCNNGG 1 cut(s) 67
BsaXI ACNNNNNCTCC 2 cut(s) 300, 330
Bse1I ACTGG 2 cut(s) 47, 546
Bse3DI GCAATG 1 cut(s) 219
Bse8I GATNNNNATC 2 cut(s) 381, 474
BseDI CCNNGG 1 cut(s) 67
BseJI GATNNNNATC 2 cut(s) 381, 474
BseMI GCAATG 1 cut(s) 219
BseNI ACTGG 2 cut(s) 47, 546
BseSI GKGCMC 2 cut(s) 221, 371
BseXI GCAGC 1 cut(s) 264
BseYI CCCAGC 1 cut(s) 19
Bsh1236I CGCG 2 cut(s) 114, 264
BshFI GGCC 2 cut(s) 351, 516
BshNI GGYRCC 1 cut(s) 503
BsiHKAI GWGCWC 2 cut(s) 371, 585
BsiWI CGTACG 1 cut(s) 341
BsnI GGCC 2 cut(s) 351, 516
Bsp1286I GDGCHC 3 cut(s) 221, 371, 585
Bsp143I GATC 2 cut(s) 382, 469
BspACI CCGC 2 cut(s) 112, 262
BspANI GGCC 2 cut(s) 351, 516
BspFNI CGCG 2 cut(s) 114, 264
BspHI TCATGA 1 cut(s) 79
BspLI GGNNCC 1 cut(s) 505
BspPI GGATC 2 cut(s) 377, 464
BspQI GCTCTTC 2 cut(s) 135, 285
BspT107I GGYRCC 1 cut(s) 503
BsrDI GCAATG 1 cut(s) 219
BsrI ACTGG 2 cut(s) 47, 546
BssECI CCNNGG 1 cut(s) 67
BssMI GATC 2 cut(s) 382, 469
BssT1I CCWWGG 1 cut(s) 67
Bst4CI ACNGT 1 cut(s) 28
Bst6I CTCTTC 2 cut(s) 135, 285
BstAPI GCANNNNNTGC 1 cut(s) 258
BstEII GGTNACC 1 cut(s) 197
BstFNI CGCG 2 cut(s) 114, 264
BstKTI GATC 2 cut(s) 385, 472
BstMBI GATC 2 cut(s) 382, 469
BstMWI GCNNNNNNNGC 2 cut(s) 258, 261
BstPI GGTNACC 1 cut(s) 197
BstSLI GKGCMC 2 cut(s) 221, 371
BstUI CGCG 2 cut(s) 114, 264
BstV1I GCAGC 1 cut(s) 264
BsuRI GGCC 2 cut(s) 351, 516
BtgZI GCGATG 1 cut(s) 431
BtsI GCAGTG 1 cut(s) 580
BtsIMutI CAGTG 2 cut(s) 539, 580
CciI TCATGA 1 cut(s) 79
Cfr13I GGNCC 3 cut(s) 58, 349, 515
Csp6I GTAC 1 cut(s) 342
CviAII CATG 6 cut(s) 34, 80, 126, 146, 296, 553
CviJI RGCY 4 cut(s) 23, 86, 351, 516
CviKI_1 RGCY 4 cut(s) 23, 86, 351, 516
CviQI GTAC 1 cut(s) 342
DpnI GATC 2 cut(s) 384, 471
DpnII GATC 2 cut(s) 382, 469
Eam1104I CTCTTC 2 cut(s) 135, 285
EarI CTCTTC 2 cut(s) 135, 285
Eco130I CCWWGG 1 cut(s) 67
Eco32I GATATC 1 cut(s) 452
Eco47I GGWCC 1 cut(s) 58
Eco91I GGTNACC 1 cut(s) 197
EcoO109I RGGNCCY 1 cut(s) 58
EcoO65I GGTNACC 1 cut(s) 197
EcoRV GATATC 1 cut(s) 452
EcoT14I CCWWGG 1 cut(s) 67
ErhI CCWWGG 1 cut(s) 67
FaeI CATG 6 cut(s) 37, 83, 129, 149, 299, 556
FalI AAGNNNNNCTT 2 cut(s) 338, 370
FatI CATG 6 cut(s) 33, 79, 125, 145, 295, 552
FblI GTMKAC 1 cut(s) 178
Fnu4HI GCNGC 3 cut(s) 112, 253, 262
Fsp4HI GCNGC 3 cut(s) 112, 253, 262
GluI GCNGC 3 cut(s) 112, 253, 262
GsaI CCCAGC 1 cut(s) 23
HaeIII GGCC 2 cut(s) 351, 516
Hin1II CATG 6 cut(s) 37, 83, 129, 149, 299, 556
HincII GTYRAC 2 cut(s) 52, 179
HindII GTYRAC 2 cut(s) 52, 179
HinfI GANTC 1 cut(s) 72
HphI GGTGA 2 cut(s) 8, 209
Hpy166II GTNNAC 4 cut(s) 52, 179, 319, 369
Hpy188I TCNGA 2 cut(s) 443, 469
Hpy188III TCNNGA 5 cut(s) 80, 137, 287, 362, 603
Hpy8I GTNNAC 4 cut(s) 52, 179, 319, 369
HpyAV CCTTC 3 cut(s) 86, 88, 340
HpyCH4III ACNGT 1 cut(s) 28
HpyCH4IV ACGT 3 cut(s) 105, 238, 435
HpyCH4V TGCA 3 cut(s) 212, 252, 369
HpyF10VI GCNNNNNNNGC 2 cut(s) 258, 261
HpySE526I ACGT 3 cut(s) 105, 238, 435
Hsp92II CATG 6 cut(s) 37, 83, 129, 149, 299, 556
Kzo9I GATC 2 cut(s) 382, 469
LguI GCTCTTC 2 cut(s) 135, 285
LmnI GCTCC 1 cut(s) 588
Lsp1109I GCAGC 1 cut(s) 264
LweI GCATC 1 cut(s) 473
MaeII ACGT 3 cut(s) 105, 238, 435
MaeIII GTNAC 1 cut(s) 197
MalI GATC 2 cut(s) 384, 471
MboI GATC 2 cut(s) 382, 469
MboII GAAGA 4 cut(s) 110, 152, 244, 302
MhlI GDGCHC 3 cut(s) 221, 371, 585
MluCI AATT 2 cut(s) 322, 492
MlyI GAGTC 1 cut(s) 66
MmeI TCCRAC 2 cut(s) 410, 421
MnlI CCTC 6 cut(s) 49, 154, 278, 367, 369, 399
MseI TTAA 1 cut(s) 392
MslI CAYNNNNRTG 1 cut(s) 207
MvnI CGCG 2 cut(s) 114, 264
MwoI GCNNNNNNNGC 2 cut(s) 258, 261
NdeII GATC 2 cut(s) 382, 469
NlaIII CATG 6 cut(s) 37, 83, 129, 149, 299, 556
NlaIV GGNNCC 1 cut(s) 505
NmuCI GTSAC 1 cut(s) 197
PagI TCATGA 1 cut(s) 79
PciSI GCTCTTC 2 cut(s) 135, 285
PcsI WCGNNNNNNNCGW 1 cut(s) 111
Pfl23II CGTACG 1 cut(s) 341
PkrI GCNGC 3 cut(s) 113, 254, 263
PleI GAGTC 1 cut(s) 66
PpsI GAGTC 1 cut(s) 66
PpuMI RGGWCCY 1 cut(s) 58
Psp5II RGGWCCY 1 cut(s) 58
PspEI GGTNACC 1 cut(s) 197
PspFI CCCAGC 1 cut(s) 19
PspLI CGTACG 1 cut(s) 341
PspN4I GGNNCC 1 cut(s) 505
PspPI GGNCC 3 cut(s) 58, 349, 515
PspPPI RGGWCCY 1 cut(s) 58
RsaI GTAC 1 cut(s) 343
RsaNI GTAC 1 cut(s) 342
RseI CAYNNNNRTG 1 cut(s) 207
SalI GTCGAC 1 cut(s) 177
SapI GCTCTTC 2 cut(s) 135, 285
SaqAI TTAA 1 cut(s) 392
SatI GCNGC 3 cut(s) 112, 253, 262
Sau3AI GATC 2 cut(s) 382, 469
Sau96I GGNCC 3 cut(s) 58, 349, 515
SchI GAGTC 1 cut(s) 66
SduI GDGCHC 3 cut(s) 221, 371, 585
SetI ASST 8 cut(s) 63, 69, 88, 108, 241, 270, 398, 438
SfaNI GCATC 1 cut(s) 473
SinI GGWCC 1 cut(s) 58
SmiMI CAYNNNNRTG 1 cut(s) 207
SmlI CTYRAG 1 cut(s) 360
SmoI CTYRAG 1 cut(s) 360
Sse9I AATT 2 cut(s) 322, 492
SsiI CCGC 2 cut(s) 112, 262
SspI AATATT 1 cut(s) 568
StyI CCWWGG 1 cut(s) 67
TaaI ACNGT 1 cut(s) 28
TaiI ACGT 3 cut(s) 108, 241, 438
TaqI TCGA 1 cut(s) 178
TasI AATT 2 cut(s) 322, 492
TauI GCSGC 2 cut(s) 114, 264
Tru1I TTAA 1 cut(s) 392
Tru9I TTAA 1 cut(s) 392
TscAI CASTG 2 cut(s) 546, 580
TseFI GTSAC 1 cut(s) 197
TseI GCWGC 1 cut(s) 252
Tsp45I GTSAC 1 cut(s) 197
TspDTI ATGAA 3 cut(s) 68, 96, 114
TspRI CASTG 2 cut(s) 546, 580
VneI GTGCAC 1 cut(s) 367
VpaK11BI GGWCC 1 cut(s) 58
XmiI GTMKAC 1 cut(s) 178
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.