Rh5AG025200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0001758
Physical Location & Seq
Forward (+)
1738849 .. 1744148
5300 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG025200.1

Sequence Viewer

Length: 291 bp
ATGAGTACTATCAGGAACATTGTCCGCAACCTCATGCGCCATAAAGAAACGATCGCGGTGTGTGCTAGTTCGATTGATCAGGATCTGAACCTAGGGCTCCTTAGTGAGGCTGAACACAAGAAAGAACATTATGAAGCATCTAGCTCCTTTACTCGTAAAGTGAAATATAGTATAGTAGGAGAGCATAGTTGTTCTTACTGTTATGTCGTGGGCATAGATGAGTATCAAAGAATATGCGGAGTAAAGACCATATTAATGGAGACTGTGGATTTGTGCTACTTGTCACTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

96

Amino Acids

10.94

Weight (kDa)

6.4

Isoelectric Point (pI)

36.39

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 56
AciI CCGC 3 cut(s) 25, 56, 237
AclWI GGATC 1 cut(s) 90
AfaI GTAC 1 cut(s) 7
AfiI CCNNNNNNNGG 1 cut(s) 106
AluBI AGCT 1 cut(s) 144
AluI AGCT 1 cut(s) 144
Alw26I GTCTC 1 cut(s) 254
AlwI GGATC 1 cut(s) 90
AlwNI CAGNNNCTG 1 cut(s) 85
ArsI GACNNNNNNTTYG 2 cut(s) 253, 285
AseI ATTAAT 1 cut(s) 254
AspA2I CCTAGG 1 cut(s) 91
AspLEI GCGC 1 cut(s) 39
AvrII CCTAGG 1 cut(s) 91
BanII GRGCYC 1 cut(s) 99
BclI TGATCA 1 cut(s) 76
BcoDI GTCTC 1 cut(s) 254
BfaI CTAG 3 cut(s) 66, 92, 141
BlnI CCTAGG 1 cut(s) 91
BmcAI AGTACT 1 cut(s) 7
BmiI GGNNCC 1 cut(s) 98
BmsI GCATC 1 cut(s) 146
BsaBI GATNNNNATC 2 cut(s) 81, 222
BsaJI CCNNGG 1 cut(s) 91
Bsc4I CCNNNNNNNGG 1 cut(s) 106
Bse8I GATNNNNATC 2 cut(s) 81, 222
BseDI CCNNGG 1 cut(s) 91
BseJI GATNNNNATC 2 cut(s) 81, 222
BseLI CCNNNNNNNGG 1 cut(s) 106
Bsh1236I CGCG 1 cut(s) 56
Bsh1285I CGRYCG 1 cut(s) 54
BsiEI CGRYCG 1 cut(s) 54
BslI CCNNNNNNNGG 1 cut(s) 106
BsmAI GTCTC 1 cut(s) 254
Bsp1286I GDGCHC 1 cut(s) 99
Bsp143I GATC 3 cut(s) 51, 76, 82
BspACI CCGC 3 cut(s) 25, 56, 237
BspFNI CGCG 1 cut(s) 56
BspLI GGNNCC 1 cut(s) 98
BspPI GGATC 1 cut(s) 90
BssECI CCNNGG 1 cut(s) 91
BssMI GATC 3 cut(s) 51, 76, 82
BssT1I CCWWGG 1 cut(s) 91
Bst4CI ACNGT 3 cut(s) 200, 265, 288
BstDEI CTNAG 1 cut(s) 101
BstENI CCTNNNNNAGG 1 cut(s) 104
BstFNI CGCG 1 cut(s) 56
BstHHI GCGC 1 cut(s) 39
BstKTI GATC 3 cut(s) 54, 79, 85
BstMAI GTCTC 1 cut(s) 254
BstMBI GATC 3 cut(s) 51, 76, 82
BstMCI CGRYCG 1 cut(s) 54
BstMWI GCNNNNNNNGC 1 cut(s) 62
BstUI CGCG 1 cut(s) 56
BstX2I RGATCY 1 cut(s) 82
BstXI CCANNNNNNTGG 1 cut(s) 256
BstYI RGATCY 1 cut(s) 82
BtsIMutI CAGTG 1 cut(s) 284
CaiI CAGNNNCTG 1 cut(s) 85
CfoI GCGC 1 cut(s) 39
Csp6I GTAC 1 cut(s) 6
CviAII CATG 1 cut(s) 34
CviJI RGCY 3 cut(s) 97, 110, 144
CviKI_1 RGCY 3 cut(s) 97, 110, 144
CviQI GTAC 1 cut(s) 6
DdeI CTNAG 1 cut(s) 101
DpnI GATC 3 cut(s) 53, 78, 84
DpnII GATC 3 cut(s) 51, 76, 82
Eco130I CCWWGG 1 cut(s) 91
Eco24I GRGCYC 1 cut(s) 99
EcoNI CCTNNNNNAGG 1 cut(s) 104
EcoT14I CCWWGG 1 cut(s) 91
EcoT38I GRGCYC 1 cut(s) 99
ErhI CCWWGG 1 cut(s) 91
FaeI CATG 1 cut(s) 37
FatI CATG 1 cut(s) 33
FbaI TGATCA 1 cut(s) 76
FriOI GRGCYC 1 cut(s) 99
FspBI CTAG 3 cut(s) 66, 92, 141
GlaI GCGC 1 cut(s) 38
HhaI GCGC 1 cut(s) 39
Hin1II CATG 1 cut(s) 37
Hin6I GCGC 1 cut(s) 37
HinP1I GCGC 1 cut(s) 37
Hpy188I TCNGA 1 cut(s) 87
Hpy188III TCNNGA 2 cut(s) 13, 80
HpyCH4III ACNGT 3 cut(s) 200, 265, 288
HpyF10VI GCNNNNNNNGC 1 cut(s) 62
HpyF3I CTNAG 1 cut(s) 101
Hsp92II CATG 1 cut(s) 37
HspAI GCGC 1 cut(s) 37
Ksp22I TGATCA 1 cut(s) 76
Kzo9I GATC 3 cut(s) 51, 76, 82
LmnI GCTCC 2 cut(s) 102, 149
LpnPI CCDG 1 cut(s) 65
LweI GCATC 1 cut(s) 146
MaeI CTAG 3 cut(s) 66, 92, 141
MaeIII GTNAC 1 cut(s) 282
MalI GATC 3 cut(s) 53, 78, 84
MboI GATC 3 cut(s) 51, 76, 82
MflI RGATCY 1 cut(s) 82
MhlI GDGCHC 1 cut(s) 99
MnlI CCTC 2 cut(s) 41, 100
MseI TTAA 1 cut(s) 254
MslI CAYNNNNRTG 1 cut(s) 254
MvnI CGCG 1 cut(s) 56
MwoI GCNNNNNNNGC 1 cut(s) 62
NdeII GATC 3 cut(s) 51, 76, 82
NlaIII CATG 1 cut(s) 37
NlaIV GGNNCC 1 cut(s) 98
NmuCI GTSAC 1 cut(s) 282
Ple19I CGATCG 1 cut(s) 54
PshBI ATTAAT 1 cut(s) 254
PspN4I GGNNCC 1 cut(s) 98
PstNI CAGNNNCTG 1 cut(s) 85
PsuI RGATCY 1 cut(s) 82
PvuI CGATCG 1 cut(s) 54
RsaI GTAC 1 cut(s) 7
RsaNI GTAC 1 cut(s) 6
RseI CAYNNNNRTG 1 cut(s) 254
SaqAI TTAA 1 cut(s) 254
Sau3AI GATC 3 cut(s) 51, 76, 82
ScaI AGTACT 1 cut(s) 7
SduI GDGCHC 1 cut(s) 99
SetI ASST 3 cut(s) 33, 93, 146
SfaNI GCATC 1 cut(s) 146
SmiMI CAYNNNNRTG 1 cut(s) 254
SsiI CCGC 3 cut(s) 25, 56, 237
SspMI CTAG 3 cut(s) 66, 92, 141
StyI CCWWGG 1 cut(s) 91
TaaI ACNGT 3 cut(s) 200, 265, 288
TaqI TCGA 1 cut(s) 71
TatI WGTACW 1 cut(s) 5
Tru1I TTAA 1 cut(s) 254
Tru9I TTAA 1 cut(s) 254
TscAI CASTG 1 cut(s) 291
TseFI GTSAC 1 cut(s) 282
Tsp45I GTSAC 1 cut(s) 282
TspDTI ATGAA 1 cut(s) 147
TspRI CASTG 1 cut(s) 291
VspI ATTAAT 1 cut(s) 254
XagI CCTNNNNNAGG 1 cut(s) 104
XmaJI CCTAGG 1 cut(s) 91
XspI CTAG 3 cut(s) 66, 92, 141
ZrmI AGTACT 1 cut(s) 7
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.