Rh5BG029500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0001758
Physical Location & Seq
Forward (+)
2088490 .. 2089721
1232 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG029500.1

Sequence Viewer

Length: 582 bp
ATGCGTACCAATCCAGTTGACGAGGACCTGAACCTTGGACTCCTCCATGAAGCTGACCAGAAGGAAGAACAGTATGAAGCATCTATCTCTCCTATTCCAATGGAGCAGCAAAAAGGTACCAAGGCAGAAGTAACTCCAATCTTCCCTGATACACCTGCACTTGTGGTAGAACAAGTAAAAAAAGATGACTACGTTTTTTGTCAACATTGTTCTAAGATCGGTGACCATAAAACTGCAATGTGCCCCAGTCAACCGAAGGGAGGTTTAATGATTTGCAGCGTTTGCCGCGACTTTTATCCGTGTAAAAATCAGGAAGAGCATGGCAACAAAGCAGAGGACTACATTTCTTGTCGAGATTGTTATACGATTGGTGACCATAAAACTGCAATGTGCCCAAATAAACCGAAGAAACGTGTAATGGTTTGCAGCGTTTGCCGCGACCTCTATCCCTGTAAAAATCAGGAAGAGCATGGCAACAATGGAGTTGAAGTAAACAATTTTTGTTATCGTTGTTTTAGGAATGGCCACTTGCCTCAAGAGTGCACAGAGGATTATGATCCAACTTTAACCTTGAACGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

193

Amino Acids

21.99

Weight (kDa)

5.28

Isoelectric Point (pI)

39.08

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 163
Acc36I ACCTGC 1 cut(s) 163
Acc65I GGTACC 1 cut(s) 116
AccB1I GGYRCC 1 cut(s) 116
AccII CGCG 2 cut(s) 288, 438
AciI CCGC 2 cut(s) 286, 436
AclWI GGATC 1 cut(s) 551
AcoI YGGCCR 1 cut(s) 523
AfaI GTAC 2 cut(s) 7, 118
AfiI CCNNNNNNNGG 1 cut(s) 260
AflIII ACRYGT 1 cut(s) 412
AgsI TTSAA 2 cut(s) 488, 574
AluBI AGCT 1 cut(s) 53
AluI AGCT 1 cut(s) 53
Alw21I GWGCWC 1 cut(s) 545
Alw44I GTGCAC 1 cut(s) 541
AlwI GGATC 1 cut(s) 551
AoxI GGCC 1 cut(s) 523
ApaLI GTGCAC 1 cut(s) 541
ApeKI GCWGC 3 cut(s) 106, 276, 426
Asp718I GGTACC 1 cut(s) 116
AspS9I GGNCC 1 cut(s) 25
AsuHPI GGTGA 2 cut(s) 233, 383
AvaII GGWCC 1 cut(s) 25
BaeGI GKGCMC 3 cut(s) 245, 395, 545
BalI TGGCCA 1 cut(s) 525
BanI GGYRCC 1 cut(s) 116
Bbv12I GWGCWC 1 cut(s) 545
BbvI GCAGC 3 cut(s) 118, 288, 438
BfuAI ACCTGC 1 cut(s) 163
BisI GCNGC 5 cut(s) 107, 277, 286, 427, 436
BlsI GCNGC 5 cut(s) 108, 278, 287, 428, 437
Bme18I GGWCC 1 cut(s) 25
BmgT120I GGNCC 1 cut(s) 25
BmiI GGNNCC 1 cut(s) 118
BmrI ACTGGG 1 cut(s) 240
BmsI GCATC 1 cut(s) 89
BmuI ACTGGG 1 cut(s) 240
BpuEI CTTGAG 1 cut(s) 519
BsaBI GATNNNNATC 1 cut(s) 555
BsaJI CCNNGG 2 cut(s) 34, 120
BsaXI ACNNNNNCTCC 2 cut(s) 474, 504
Bsc4I CCNNNNNNNGG 1 cut(s) 260
Bse1I ACTGG 2 cut(s) 14, 246
Bse3DI GCAATG 2 cut(s) 243, 393
Bse8I GATNNNNATC 1 cut(s) 555
BseDI CCNNGG 2 cut(s) 34, 120
BseJI GATNNNNATC 1 cut(s) 555
BseLI CCNNNNNNNGG 1 cut(s) 260
BseMI GCAATG 2 cut(s) 243, 393
BseNI ACTGG 2 cut(s) 14, 246
BseRI GAGGAG 1 cut(s) 32
BseSI GKGCMC 3 cut(s) 245, 395, 545
BseXI GCAGC 3 cut(s) 118, 288, 438
BsgI GTGCAG 1 cut(s) 141
Bsh1236I CGCG 2 cut(s) 288, 438
BshFI GGCC 1 cut(s) 525
BshNI GGYRCC 1 cut(s) 116
BsiHKAI GWGCWC 1 cut(s) 545
BslI CCNNNNNNNGG 1 cut(s) 260
BsnI GGCC 1 cut(s) 525
Bsp1286I GDGCHC 3 cut(s) 245, 395, 545
Bsp143I GATC 2 cut(s) 216, 556
BspACI CCGC 2 cut(s) 286, 436
BspANI GGCC 1 cut(s) 525
BspFNI CGCG 2 cut(s) 288, 438
BspLI GGNNCC 1 cut(s) 118
BspMI ACCTGC 1 cut(s) 163
BspPI GGATC 1 cut(s) 551
BspQI GCTCTTC 2 cut(s) 309, 459
BspT107I GGYRCC 1 cut(s) 116
BsrDI GCAATG 2 cut(s) 243, 393
BsrI ACTGG 2 cut(s) 14, 246
BssECI CCNNGG 2 cut(s) 34, 120
BssMI GATC 2 cut(s) 216, 556
BssT1I CCWWGG 2 cut(s) 34, 120
Bst4CI ACNGT 1 cut(s) 72
Bst6I CTCTTC 2 cut(s) 309, 459
BstAPI GCANNNNNTGC 2 cut(s) 282, 432
BstDEI CTNAG 1 cut(s) 213
BstEII GGTNACC 2 cut(s) 221, 371
BstFNI CGCG 2 cut(s) 288, 438
BstKTI GATC 2 cut(s) 219, 559
BstMBI GATC 2 cut(s) 216, 556
BstMWI GCNNNNNNNGC 4 cut(s) 282, 285, 432, 435
BstPI GGTNACC 2 cut(s) 221, 371
BstSLI GKGCMC 3 cut(s) 245, 395, 545
BstUI CGCG 2 cut(s) 288, 438
BstV1I GCAGC 3 cut(s) 118, 288, 438
BsuRI GGCC 1 cut(s) 525
BveI ACCTGC 1 cut(s) 163
Cfr13I GGNCC 1 cut(s) 25
Csp6I GTAC 2 cut(s) 6, 117
CviAII CATG 3 cut(s) 47, 320, 470
CviJI RGCY 2 cut(s) 53, 525
CviKI_1 RGCY 2 cut(s) 53, 525
CviQI GTAC 2 cut(s) 6, 117
DdeI CTNAG 1 cut(s) 213
DpnI GATC 2 cut(s) 218, 558
DpnII GATC 2 cut(s) 216, 556
EaeI YGGCCR 1 cut(s) 523
Eam1104I CTCTTC 2 cut(s) 309, 459
EarI CTCTTC 2 cut(s) 309, 459
Eco130I CCWWGG 2 cut(s) 34, 120
Eco47I GGWCC 1 cut(s) 25
Eco91I GGTNACC 2 cut(s) 221, 371
EcoO109I RGGNCCY 1 cut(s) 25
EcoO65I GGTNACC 2 cut(s) 221, 371
EcoT14I CCWWGG 2 cut(s) 34, 120
ErhI CCWWGG 2 cut(s) 34, 120
FaeI CATG 3 cut(s) 50, 323, 473
FaiI YATR 8 cut(s) 48, 75, 228, 321, 363, 378, 471, 555
FatI CATG 3 cut(s) 46, 319, 469
Fnu4HI GCNGC 5 cut(s) 107, 277, 286, 427, 436
Fsp4HI GCNGC 5 cut(s) 107, 277, 286, 427, 436
GluI GCNGC 5 cut(s) 107, 277, 286, 427, 436
HaeIII GGCC 1 cut(s) 525
Hin1II CATG 3 cut(s) 50, 323, 473
HincII GTYRAC 3 cut(s) 19, 203, 251
HindII GTYRAC 3 cut(s) 19, 203, 251
HinfI GANTC 1 cut(s) 39
HphI GGTGA 2 cut(s) 233, 383
Hpy166II GTNNAC 5 cut(s) 19, 203, 251, 493, 543
Hpy188III TCNNGA 4 cut(s) 311, 353, 461, 536
Hpy8I GTNNAC 5 cut(s) 19, 203, 251, 493, 543
HpyAV CCTTC 2 cut(s) 55, 250
HpyCH4III ACNGT 1 cut(s) 72
HpyCH4IV ACGT 2 cut(s) 192, 412
HpyCH4V TGCA 6 cut(s) 158, 236, 276, 386, 426, 543
HpyF10VI GCNNNNNNNGC 4 cut(s) 282, 285, 432, 435
HpyF3I CTNAG 1 cut(s) 213
HpySE526I ACGT 2 cut(s) 192, 412
Hsp92II CATG 3 cut(s) 50, 323, 473
KpnI GGTACC 1 cut(s) 120
Kzo9I GATC 2 cut(s) 216, 556
LguI GCTCTTC 2 cut(s) 309, 459
LmnI GCTCC 1 cut(s) 103
LpnPI CCDG 9 cut(s) 27, 41, 71, 159, 168, 259, 296, 446, 463
Lsp1109I GCAGC 3 cut(s) 118, 288, 438
LweI GCATC 1 cut(s) 89
MaeII ACGT 2 cut(s) 192, 412
MaeIII GTNAC 3 cut(s) 130, 221, 371
MalI GATC 2 cut(s) 218, 558
MboI GATC 2 cut(s) 216, 556
MboII GAAGA 5 cut(s) 77, 133, 326, 418, 476
MhlI GDGCHC 3 cut(s) 245, 395, 545
MlsI TGGCCA 1 cut(s) 525
MluCI AATT 1 cut(s) 496
MluNI TGGCCA 1 cut(s) 525
MlyI GAGTC 1 cut(s) 33
MnlI CCTC 7 cut(s) 16, 53, 254, 328, 452, 541, 543
Mox20I TGGCCA 1 cut(s) 525
MscI TGGCCA 1 cut(s) 525
MseI TTAA 2 cut(s) 266, 566
Msp20I TGGCCA 1 cut(s) 525
MvnI CGCG 2 cut(s) 288, 438
MwoI GCNNNNNNNGC 4 cut(s) 282, 285, 432, 435
NdeII GATC 2 cut(s) 216, 556
NlaIII CATG 3 cut(s) 50, 323, 473
NlaIV GGNNCC 1 cut(s) 118
NmuCI GTSAC 2 cut(s) 221, 371
PaqCI CACCTGC 1 cut(s) 163
PciSI GCTCTTC 2 cut(s) 309, 459
PkrI GCNGC 5 cut(s) 108, 278, 287, 428, 437
PleI GAGTC 1 cut(s) 33
PpsI GAGTC 1 cut(s) 33
PpuMI RGGWCCY 1 cut(s) 25
Psp5II RGGWCCY 1 cut(s) 25
PspEI GGTNACC 2 cut(s) 221, 371
PspN4I GGNNCC 1 cut(s) 118
PspPI GGNCC 1 cut(s) 25
PspPPI RGGWCCY 1 cut(s) 25
RsaI GTAC 2 cut(s) 7, 118
RsaNI GTAC 2 cut(s) 6, 117
SapI GCTCTTC 2 cut(s) 309, 459
SaqAI TTAA 2 cut(s) 266, 566
SatI GCNGC 5 cut(s) 107, 277, 286, 427, 436
Sau3AI GATC 2 cut(s) 216, 556
Sau96I GGNCC 1 cut(s) 25
SchI GAGTC 1 cut(s) 33
SduI GDGCHC 3 cut(s) 245, 395, 545
SfaNI GCATC 1 cut(s) 89
SinI GGWCC 1 cut(s) 25
SmlI CTYRAG 1 cut(s) 534
SmoI CTYRAG 1 cut(s) 534
Sse9I AATT 1 cut(s) 496
SsiI CCGC 2 cut(s) 286, 436
StyI CCWWGG 2 cut(s) 34, 120
TaaI ACNGT 1 cut(s) 72
TaiI ACGT 2 cut(s) 195, 415
TaqI TCGA 1 cut(s) 352
TasI AATT 1 cut(s) 496
TauI GCSGC 2 cut(s) 288, 438
Tru1I TTAA 2 cut(s) 266, 566
Tru9I TTAA 2 cut(s) 266, 566
TseFI GTSAC 2 cut(s) 221, 371
TseI GCWGC 3 cut(s) 106, 276, 426
Tsp45I GTSAC 2 cut(s) 221, 371
TspDTI ATGAA 2 cut(s) 63, 90
TspGWI ACGGA 1 cut(s) 288
VneI GTGCAC 1 cut(s) 541
VpaK11BI GGWCC 1 cut(s) 25
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.