RLG00000030356

Transcription initiation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0001612
Physical Location & Seq
Reverse (-)
60034871 .. 60038503
3633 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030356

Sequence Viewer

Length: 624 bp
ATGAATGGATCAGAAAACATAGTGCAATGTATGCGTAGAGCTTCTCCACCACCAAGTAAGAAGCAAAAGGTAGCTGATGATGAGATCGGCACAGATCAAGAAGGCATTGAGCAACTCTGTGACGTCGTTTCTGTCAGTGGAGTCAATCTTGTGGAAGAGCAAGAGCGCATGCTGTTGCTTTCGGGGCCCAAGATGCAGCAAGCATCTCGAAAACTTGTGTTTCAAGAAAACCAAGAAGGGCTGGTTTTGCAGAAGATTCCCCTACAGAAAAAGCGGATCAGCAAGGCGGATCAGCTCGCCTGGGAGAAGCGTCGAGGTAAAGATGTAAACCACAAGGCTACATCAACTACTGGAAGAACAATGAATGGCAATGTAGAAGCAGAGAAAAGGGGTAGTGTAGAAGCTTTGGTGTCTGCAGCTGGGAGTGCTGTTGGAAAGTTTGGGAGGAATGCCCAAGTTATGATGATGCCTCAAACAAAAGTGGCTAGTACTCGTAGTATAACTGTCAAGGATGTGATTGCTGTTCTCGAAAAGGAACCTCAGATGTCCAAGTCTACTCTGATCTATTTACTAACGAACACTGCAGAATGGCTGTTTGGAGTTCCTAGGATCAATTTCTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000428 GO:0001650 GO:0003674 GO:0003676 GO:0003677 GO:0003712 GO:0003713 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005667 GO:0005669 GO:0005730 GO:0005911 GO:0006139 GO:0006351 GO:0006352 GO:0006355 GO:0006357 GO:0006366 GO:0006367 GO:0006725 GO:0006807 GO:0008134 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009506 GO:0009889 GO:0009891 GO:0009893 GO:0009966 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0010646 GO:0016070 GO:0016591 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0023051 GO:0030054 GO:0030880 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031974 GO:0031981 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044452 GO:0044464 GO:0044798 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048522 GO:0048583 GO:0050789 GO:0050794 GO:0051059 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0055029 GO:0055044 GO:0060255 GO:0061695 GO:0065007 GO:0070013 GO:0071704 GO:0080090 GO:0090304 GO:0090575 GO:0097159 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1901796 GO:1902494 GO:1902531 GO:1902680 GO:1903506 GO:1903508 GO:1990234 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03022 ko05016 ko05168 map03022 map05016 map05168
Pfam Domains
Protein Families
RST TAF4

Protein Analysis

208

Amino Acids

22.89

Weight (kDa)

9.49

Isoelectric Point (pI)

47.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TAF4 PF05236 40 - 94 4.5e-06 Transcription initiation factor TFIID component TAF4 family
TAF4 PF05236 102 - 189 3.7e-08 Transcription initiation factor TFIID component TAF4 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 126
AccI GTMKAC 1 cut(s) 554
AciI CCGC 2 cut(s) 274, 287
AclWI GGATC 4 cut(s) 16, 284, 297, 617
AcyI GRCGYC 1 cut(s) 123
AfaI GTAC 1 cut(s) 490
AgsI TTSAA 1 cut(s) 224
AjnI CCWGG 1 cut(s) 299
AjuI GAANNNNNNNTTGG 2 cut(s) 579, 611
AluBI AGCT 5 cut(s) 41, 74, 295, 404, 419
AluI AGCT 5 cut(s) 41, 74, 295, 404, 419
AlwI GGATC 4 cut(s) 16, 284, 297, 617
AoxI GGCC 1 cut(s) 185
ApaI GGGCCC 1 cut(s) 189
ApeKI GCWGC 2 cut(s) 196, 416
AspA2I CCTAGG 1 cut(s) 605
AspLEI GCGC 1 cut(s) 168
AspS9I GGNCC 2 cut(s) 185, 186
AvrII CCTAGG 1 cut(s) 605
BaeGI GKGCMC 1 cut(s) 189
BanII GRGCYC 1 cut(s) 189
BbvI GCAGC 2 cut(s) 208, 428
BcgI CGANNNNNNTGC 2 cut(s) 188, 222
BciT130I CCWGG 1 cut(s) 301
BfaI CTAG 2 cut(s) 486, 606
BfmI CTRYAG 3 cut(s) 263, 414, 582
BisI GCNGC 2 cut(s) 197, 417
BlnI CCTAGG 1 cut(s) 605
BlsI GCNGC 2 cut(s) 198, 418
BmcAI AGTACT 1 cut(s) 490
Bme1390I CCNGG 1 cut(s) 301
BmgT120I GGNCC 2 cut(s) 185, 186
BmiI GGNNCC 3 cut(s) 186, 187, 537
BmrFI CCNGG 1 cut(s) 301
BmsI GCATC 3 cut(s) 183, 212, 456
BsaHI GRCGYC 1 cut(s) 123
BsaJI CCNNGG 2 cut(s) 300, 605
Bse1I ACTGG 1 cut(s) 355
Bse3DI GCAATG 2 cut(s) 32, 376
BseBI CCWGG 1 cut(s) 301
BseDI CCNNGG 2 cut(s) 300, 605
BseGI GGATG 1 cut(s) 517
BseMI GCAATG 2 cut(s) 32, 376
BseMII CTCAG 1 cut(s) 554
BseNI ACTGG 1 cut(s) 355
BseSI GKGCMC 1 cut(s) 189
BseXI GCAGC 2 cut(s) 208, 428
BseYI CCCAGC 1 cut(s) 419
BshFI GGCC 1 cut(s) 187
BsmI GAATGC 1 cut(s) 454
BsnI GGCC 1 cut(s) 187
Bsp120I GGGCCC 1 cut(s) 185
Bsp1286I GDGCHC 1 cut(s) 189
Bsp143I GATC 7 cut(s) 8, 84, 94, 276, 289, 561, 609
BspACI CCGC 2 cut(s) 274, 287
BspANI GGCC 1 cut(s) 187
BspCNI CTCAG 1 cut(s) 553
BspLI GGNNCC 3 cut(s) 186, 187, 537
BspMAI CTGCAG 2 cut(s) 418, 586
BspPI GGATC 4 cut(s) 16, 284, 297, 617
BspQI GCTCTTC 1 cut(s) 150
BsrDI GCAATG 2 cut(s) 32, 376
BsrI ACTGG 1 cut(s) 355
BssECI CCNNGG 2 cut(s) 300, 605
BssMI GATC 7 cut(s) 8, 84, 94, 276, 289, 561, 609
BssNI GRCGYC 1 cut(s) 123
BssT1I CCWWGG 1 cut(s) 605
Bst2UI CCWGG 1 cut(s) 301
Bst4CI ACNGT 1 cut(s) 505
Bst6I CTCTTC 1 cut(s) 150
BstACI GRCGYC 1 cut(s) 123
BstAPI GCANNNNNTGC 1 cut(s) 31
BstC8I GCNNGC 3 cut(s) 170, 201, 297
BstDEI CTNAG 1 cut(s) 540
BstF5I GGATG 1 cut(s) 517
BstHHI GCGC 1 cut(s) 168
BstKTI GATC 7 cut(s) 11, 87, 97, 279, 292, 564, 612
BstMBI GATC 7 cut(s) 8, 84, 94, 276, 289, 561, 609
BstMWI GCNNNNNNNGC 5 cut(s) 31, 184, 193, 247, 425
BstNI CCWGG 1 cut(s) 301
BstNSI RCATGY 1 cut(s) 172
BstSCI CCNGG 1 cut(s) 299
BstSFI CTRYAG 3 cut(s) 263, 414, 582
BstSLI GKGCMC 1 cut(s) 189
BstV1I GCAGC 2 cut(s) 208, 428
BsuRI GGCC 1 cut(s) 187
BtsCI GGATG 1 cut(s) 517
BtsI GCAGTG 1 cut(s) 579
BtsIMutI CAGTG 2 cut(s) 142, 579
Cac8I GCNNGC 3 cut(s) 170, 201, 297
CfoI GCGC 1 cut(s) 168
Cfr13I GGNCC 2 cut(s) 185, 186
CseI GACGC 1 cut(s) 299
Csp6I GTAC 1 cut(s) 489
CviAII CATG 1 cut(s) 169
CviQI GTAC 1 cut(s) 489
DdeI CTNAG 1 cut(s) 540
DpnI GATC 7 cut(s) 10, 86, 96, 278, 291, 563, 611
DpnII GATC 7 cut(s) 8, 84, 94, 276, 289, 561, 609
Eam1104I CTCTTC 1 cut(s) 150
EarI CTCTTC 1 cut(s) 150
EciI GGCGGA 1 cut(s) 302
Eco130I CCWWGG 1 cut(s) 605
Eco24I GRGCYC 1 cut(s) 189
EcoO109I RGGNCCY 1 cut(s) 185
EcoRII CCWGG 1 cut(s) 299
EcoT14I CCWWGG 1 cut(s) 605
EcoT38I GRGCYC 1 cut(s) 189
ErhI CCWWGG 1 cut(s) 605
FaeI CATG 1 cut(s) 172
FaiI YATR 5 cut(s) 20, 32, 170, 461, 500
FatI CATG 1 cut(s) 168
FblI GTMKAC 1 cut(s) 554
Fnu4HI GCNGC 2 cut(s) 197, 417
FokI GGATG 1 cut(s) 524
FriOI GRGCYC 1 cut(s) 189
Fsp4HI GCNGC 2 cut(s) 197, 417
FspBI CTAG 2 cut(s) 486, 606
GlaI GCGC 1 cut(s) 167
GluI GCNGC 2 cut(s) 197, 417
GsaI CCCAGC 1 cut(s) 423
HaeIII GGCC 1 cut(s) 187
HgaI GACGC 1 cut(s) 299
HhaI GCGC 1 cut(s) 168
Hin1I GRCGYC 1 cut(s) 123
Hin1II CATG 1 cut(s) 172
Hin6I GCGC 1 cut(s) 166
HinP1I GCGC 1 cut(s) 166
HindIII AAGCTT 1 cut(s) 402
HinfI GANTC 2 cut(s) 141, 256
Hpy166II GTNNAC 2 cut(s) 328, 555
Hpy188I TCNGA 3 cut(s) 13, 543, 561
Hpy188III TCNNGA 4 cut(s) 98, 207, 224, 527
Hpy8I GTNNAC 2 cut(s) 328, 555
Hpy99I CGWCG 2 cut(s) 128, 315
HpyAV CCTTC 2 cut(s) 95, 230
HpyCH4III ACNGT 1 cut(s) 505
HpyCH4IV ACGT 1 cut(s) 123
HpyCH4V TGCA 5 cut(s) 25, 196, 250, 416, 584
HpyF10VI GCNNNNNNNGC 5 cut(s) 31, 184, 193, 247, 425
HpyF3I CTNAG 1 cut(s) 540
HpySE526I ACGT 1 cut(s) 123
Hsp92I GRCGYC 1 cut(s) 123
Hsp92II CATG 1 cut(s) 172
HspAI GCGC 1 cut(s) 166
Kzo9I GATC 7 cut(s) 8, 84, 94, 276, 289, 561, 609
LguI GCTCTTC 1 cut(s) 150
LpnPI CCDG 5 cut(s) 227, 286, 313, 336, 405
Lsp1109I GCAGC 2 cut(s) 208, 428
LweI GCATC 3 cut(s) 183, 212, 456
MaeI CTAG 2 cut(s) 486, 606
MaeII ACGT 1 cut(s) 123
MaeIII GTNAC 1 cut(s) 119
MalI GATC 7 cut(s) 10, 86, 96, 278, 291, 563, 611
MboI GATC 7 cut(s) 8, 84, 94, 276, 289, 561, 609
MboII GAAGA 3 cut(s) 167, 265, 366
MhlI GDGCHC 1 cut(s) 189
MluCI AATT 1 cut(s) 613
MlyI GAGTC 1 cut(s) 150
MmeI TCCRAC 1 cut(s) 412
MnlI CCTC 4 cut(s) 308, 438, 480, 549
MseI TTAA 1 cut(s) 622
MspA1I CMGCKG 1 cut(s) 419
MspR9I CCNGG 1 cut(s) 301
Mva1269I GAATGC 1 cut(s) 454
MvaI CCWGG 1 cut(s) 301
MwoI GCNNNNNNNGC 5 cut(s) 31, 184, 193, 247, 425
NdeII GATC 7 cut(s) 8, 84, 94, 276, 289, 561, 609
NlaIII CATG 1 cut(s) 172
NlaIV GGNNCC 3 cut(s) 186, 187, 537
NmuCI GTSAC 1 cut(s) 119
NspI RCATGY 1 cut(s) 172
PaeI GCATGC 1 cut(s) 172
PciSI GCTCTTC 1 cut(s) 150
PctI GAATGC 1 cut(s) 454
PfeI GAWTC 1 cut(s) 256
PkrI GCNGC 2 cut(s) 198, 418
PleI GAGTC 1 cut(s) 149
PpsI GAGTC 1 cut(s) 149
Psp6I CCWGG 1 cut(s) 299
PspFI CCCAGC 1 cut(s) 419
PspGI CCWGG 1 cut(s) 299
PspN4I GGNNCC 3 cut(s) 186, 187, 537
PspOMI GGGCCC 1 cut(s) 185
PspPI GGNCC 2 cut(s) 185, 186
PstI CTGCAG 2 cut(s) 418, 586
PvuII CAGCTG 1 cut(s) 419
RsaI GTAC 1 cut(s) 490
RsaNI GTAC 1 cut(s) 489
SapI GCTCTTC 1 cut(s) 150
SaqAI TTAA 1 cut(s) 622
SatI GCNGC 2 cut(s) 197, 417
Sau3AI GATC 7 cut(s) 8, 84, 94, 276, 289, 561, 609
Sau96I GGNCC 2 cut(s) 185, 186
ScaI AGTACT 1 cut(s) 490
SchI GAGTC 1 cut(s) 150
ScrFI CCNGG 1 cut(s) 301
SduI GDGCHC 1 cut(s) 189
SetI ASST 9 cut(s) 43, 72, 76, 126, 297, 319, 406, 421, 541
SfaNI GCATC 3 cut(s) 183, 212, 456
SfcI CTRYAG 3 cut(s) 263, 414, 582
SphI GCATGC 1 cut(s) 172
Sse9I AATT 1 cut(s) 613
SsiI CCGC 2 cut(s) 274, 287
SspMI CTAG 2 cut(s) 486, 606
StyD4I CCNGG 1 cut(s) 299
StyI CCWWGG 1 cut(s) 605
TaaI ACNGT 1 cut(s) 505
TaiI ACGT 1 cut(s) 126
TaqI TCGA 3 cut(s) 208, 313, 528
TasI AATT 1 cut(s) 613
TatI WGTACW 1 cut(s) 488
TfiI GAWTC 1 cut(s) 256
Tru1I TTAA 1 cut(s) 622
Tru9I TTAA 1 cut(s) 622
TscAI CASTG 2 cut(s) 142, 586
TseFI GTSAC 1 cut(s) 119
TseI GCWGC 2 cut(s) 196, 416
Tsp45I GTSAC 1 cut(s) 119
TspDTI ATGAA 2 cut(s) 17, 377
TspRI CASTG 2 cut(s) 142, 586
XceI RCATGY 1 cut(s) 172
XmaJI CCTAGG 1 cut(s) 605
XmiI GTMKAC 1 cut(s) 554
XspI CTAG 2 cut(s) 486, 606
ZraI GACGTC 1 cut(s) 124
ZrmI AGTACT 1 cut(s) 490
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.