RLG00000030371

Transcription initiation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0001612
Physical Location & Seq
Reverse (-)
60200243 .. 60201372
1130 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030371

Sequence Viewer

Length: 732 bp
ATGATAAGATCGACACAGATCAAGAAGGCATTGAGCAACTCCGCGCCGTCGTTTCTGTTAGTGGAGTCAATCTTGTGGAGGAGCAATGCTGTTGCTTTTGGGGCCCAAGAAGCAGCAAGCATCTCGCAAACTTGTGCTTCAAGAAAACCAAGAAGGGCTGGTTTTGCAGATGATTCCCTTGCAGAAGAAGTTGGCGGGAATCATGGGGAGGAAATTATGATTCTCAACAAGAAAGCTATGGAAGAGTTGCAGGAAAAGGCTCAGAAGCTTAATGAAGCTAAGGTTAGCACTAATGGATTTGAGGGTGTTAAGGACAAACATGATGATGGAGATGACAAAATGAGGAGAAGTACAGCAGCAGCAAATGTTGCTGCCCTTGCTGCTCTTGGAGGACATGACATATTGGCGAAATGGCAACTTAAGGCGGATCAGCTGGCCCGGGAGAAGCGTCGAGGTAAAGATGTAAACCCCCAGGCTACATCAACTATTGGAAGAACAATGAATGGCAATGTAGAAGCGGAGAAAAGGGGCGGTGTAGAAGCTTTGGTGTCTACAGCTGGAAGTGCTGTCGGAAAGTTTGGGAGGAATGCCCAAGTTATGATGATGCCTCAAACAAAGGTGGCTAGTACCCGTAGTGTATCTGTGAAGGATGTGATTGCTGTTCTGGAAAAGGAACCTCAGATGTCCAAGTCTTCTCTGATCTATTTGCTAATGAACACTGCTAGCCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000428 GO:0001650 GO:0003674 GO:0003676 GO:0003677 GO:0003712 GO:0003713 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005667 GO:0005669 GO:0005730 GO:0005911 GO:0006139 GO:0006351 GO:0006352 GO:0006355 GO:0006357 GO:0006366 GO:0006367 GO:0006725 GO:0006807 GO:0008134 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009506 GO:0009889 GO:0009891 GO:0009893 GO:0009966 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0010646 GO:0016070 GO:0016591 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0023051 GO:0030054 GO:0030880 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031974 GO:0031981 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044452 GO:0044464 GO:0044798 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048522 GO:0048583 GO:0050789 GO:0050794 GO:0051059 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0055029 GO:0055044 GO:0060255 GO:0061695 GO:0065007 GO:0070013 GO:0071704 GO:0080090 GO:0090304 GO:0090575 GO:0097159 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1901796 GO:1902494 GO:1902531 GO:1902680 GO:1903506 GO:1903508 GO:1990234 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03022 ko05016 ko05168 map03022 map05016 map05168
Pfam Domains
Protein Families
RST TAF4

Protein Analysis

244

Amino Acids

26.13

Weight (kDa)

9.59

Isoelectric Point (pI)

44.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TAF4 PF05236 77 - 235 7.4e-12 Transcription initiation factor TFIID component TAF4 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 551
AccII CGCG 1 cut(s) 44
AciI CCGC 5 cut(s) 42, 195, 425, 518, 531
AclWI GGATC 1 cut(s) 435
AfaI GTAC 2 cut(s) 352, 628
AflII CTTAAG 1 cut(s) 419
AgsI TTSAA 1 cut(s) 141
AjnI CCWGG 1 cut(s) 471
AluBI AGCT 6 cut(s) 236, 268, 278, 433, 542, 557
AluI AGCT 6 cut(s) 236, 268, 278, 433, 542, 557
AlwI GGATC 1 cut(s) 435
Ama87I CYCGRG 1 cut(s) 438
AoxI GGCC 2 cut(s) 102, 435
ApaI GGGCCC 1 cut(s) 106
ApeKI GCWGC 5 cut(s) 113, 356, 359, 371, 380
AspLEI GCGC 1 cut(s) 46
AspS9I GGNCC 3 cut(s) 102, 103, 436
AsuC2I CCSGG 2 cut(s) 439, 440
AsuNHI GCTAGC 1 cut(s) 722
AvaI CYCGRG 1 cut(s) 438
BaeGI GKGCMC 1 cut(s) 106
BanII GRGCYC 1 cut(s) 106
BbsI GAAGAC 1 cut(s) 684
BbvI GCAGC 5 cut(s) 125, 358, 367, 368, 371
BccI CCATC 1 cut(s) 320
BceAI ACGGC 1 cut(s) 31
BcgI CGANNNNNNTGC 2 cut(s) 105, 139
BciT130I CCWGG 1 cut(s) 473
BcnI CCSGG 2 cut(s) 439, 440
BfaI CTAG 2 cut(s) 624, 723
BfmI CTRYAG 1 cut(s) 552
BfrI CTTAAG 1 cut(s) 419
BisI GCNGC 5 cut(s) 114, 357, 360, 372, 381
BlsI GCNGC 5 cut(s) 115, 358, 361, 373, 382
Bme1390I CCNGG 3 cut(s) 439, 440, 473
BmeT110I CYCGRG 1 cut(s) 438
BmgT120I GGNCC 3 cut(s) 102, 103, 436
BmiI GGNNCC 3 cut(s) 103, 104, 675
BmrFI CCNGG 3 cut(s) 439, 440, 473
BmsI GCATC 2 cut(s) 129, 594
BmtI GCTAGC 1 cut(s) 726
BpiI GAAGAC 1 cut(s) 684
Bpu10I CCTNAGC 1 cut(s) 279
BpuMI CCSGG 2 cut(s) 439, 440
BsaJI CCNNGG 2 cut(s) 438, 471
Bse118I RCCGGY 1 cut(s) 726
Bse3DI GCAATG 2 cut(s) 91, 514
BseBI CCWGG 1 cut(s) 473
BseDI CCNNGG 2 cut(s) 438, 471
BseGI GGATG 1 cut(s) 655
BseMI GCAATG 2 cut(s) 91, 514
BseMII CTCAG 2 cut(s) 275, 692
BseRI GAGGAG 2 cut(s) 94, 358
BseSI GKGCMC 1 cut(s) 106
BseXI GCAGC 5 cut(s) 125, 358, 367, 368, 371
Bsh1236I CGCG 1 cut(s) 44
BshFI GGCC 2 cut(s) 104, 437
BsiHKCI CYCGRG 1 cut(s) 438
BsiSI CCGG 2 cut(s) 439, 727
BsmI GAATGC 1 cut(s) 592
BsnI GGCC 2 cut(s) 104, 437
BsoBI CYCGRG 1 cut(s) 438
Bsp120I GGGCCC 1 cut(s) 102
Bsp1286I GDGCHC 1 cut(s) 106
Bsp143I GATC 4 cut(s) 8, 18, 427, 699
BspACI CCGC 5 cut(s) 42, 195, 425, 518, 531
BspANI GGCC 2 cut(s) 104, 437
BspCNI CTCAG 2 cut(s) 274, 691
BspFNI CGCG 1 cut(s) 44
BspLI GGNNCC 3 cut(s) 103, 104, 675
BspOI GCTAGC 1 cut(s) 726
BspPI GGATC 1 cut(s) 435
BspTI CTTAAG 1 cut(s) 419
BsrDI GCAATG 2 cut(s) 91, 514
BsrFI RCCGGY 1 cut(s) 726
BssAI RCCGGY 1 cut(s) 726
BssECI CCNNGG 2 cut(s) 438, 471
BssMI GATC 4 cut(s) 8, 18, 427, 699
Bst2UI CCWGG 1 cut(s) 473
Bst6I CTCTTC 1 cut(s) 237
BstAFI CTTAAG 1 cut(s) 419
BstAPI GCANNNNNTGC 1 cut(s) 368
BstC8I GCNNGC 3 cut(s) 118, 435, 724
BstDEI CTNAG 3 cut(s) 261, 279, 678
BstF5I GGATG 1 cut(s) 655
BstFNI CGCG 1 cut(s) 44
BstHHI GCGC 1 cut(s) 46
BstKTI GATC 4 cut(s) 11, 21, 430, 702
BstMBI GATC 4 cut(s) 8, 18, 427, 699
BstMWI GCNNNNNNNGC 7 cut(s) 101, 110, 164, 368, 377, 380, 563
BstNI CCWGG 1 cut(s) 473
BstSCI CCNGG 3 cut(s) 437, 438, 471
BstSFI CTRYAG 1 cut(s) 552
BstSLI GKGCMC 1 cut(s) 106
BstUI CGCG 1 cut(s) 44
BstV1I GCAGC 5 cut(s) 125, 358, 367, 368, 371
BstV2I GAAGAC 1 cut(s) 684
BsuRI GGCC 2 cut(s) 104, 437
BtsCI GGATG 1 cut(s) 655
BtsI GCAGTG 1 cut(s) 717
BtsIMutI CAGTG 1 cut(s) 717
Cac8I GCNNGC 3 cut(s) 118, 435, 724
CfoI GCGC 1 cut(s) 46
Cfr10I RCCGGY 1 cut(s) 726
Cfr13I GGNCC 3 cut(s) 102, 103, 436
Cfr9I CCCGGG 1 cut(s) 438
CseI GACGC 1 cut(s) 437
Csp6I GTAC 2 cut(s) 351, 627
CviAII CATG 3 cut(s) 203, 320, 395
CviQI GTAC 2 cut(s) 351, 627
DdeI CTNAG 3 cut(s) 261, 279, 678
DpnI GATC 4 cut(s) 10, 20, 429, 701
DpnII GATC 4 cut(s) 8, 18, 427, 699
Eam1104I CTCTTC 1 cut(s) 237
EarI CTCTTC 1 cut(s) 237
EciI GGCGGA 1 cut(s) 440
Eco24I GRGCYC 1 cut(s) 106
Eco88I CYCGRG 1 cut(s) 438
EcoO109I RGGNCCY 1 cut(s) 102
EcoRII CCWGG 1 cut(s) 471
EcoT38I GRGCYC 1 cut(s) 106
FaeI CATG 3 cut(s) 206, 323, 398
FaiI YATR 7 cut(s) 204, 218, 239, 321, 396, 401, 599
FatI CATG 3 cut(s) 202, 319, 394
FauI CCCGC 1 cut(s) 188
FblI GTMKAC 1 cut(s) 551
Fnu4HI GCNGC 5 cut(s) 114, 357, 360, 372, 381
FokI GGATG 1 cut(s) 662
FriOI GRGCYC 1 cut(s) 106
Fsp4HI GCNGC 5 cut(s) 114, 357, 360, 372, 381
FspBI CTAG 2 cut(s) 624, 723
GlaI GCGC 1 cut(s) 45
GluI GCNGC 5 cut(s) 114, 357, 360, 372, 381
HaeIII GGCC 2 cut(s) 104, 437
HapII CCGG 2 cut(s) 439, 727
HgaI GACGC 1 cut(s) 437
HhaI GCGC 1 cut(s) 46
Hin1II CATG 3 cut(s) 206, 323, 398
Hin6I GCGC 1 cut(s) 44
HinP1I GCGC 1 cut(s) 44
HindIII AAGCTT 2 cut(s) 266, 540
HinfI GANTC 4 cut(s) 65, 173, 199, 220
HpaII CCGG 2 cut(s) 439, 727
Hpy166II GTNNAC 2 cut(s) 466, 552
Hpy188I TCNGA 4 cut(s) 264, 572, 681, 699
Hpy188III TCNNGA 3 cut(s) 22, 141, 665
Hpy8I GTNNAC 2 cut(s) 466, 552
Hpy99I CGWCG 2 cut(s) 52, 453
HpyAV CCTTC 3 cut(s) 19, 147, 640
HpyCH4V TGCA 3 cut(s) 167, 182, 250
HpyF10VI GCNNNNNNNGC 7 cut(s) 101, 110, 164, 368, 377, 380, 563
HpyF3I CTNAG 3 cut(s) 261, 279, 678
Hsp92II CATG 3 cut(s) 206, 323, 398
HspAI GCGC 1 cut(s) 44
Kzo9I GATC 4 cut(s) 8, 18, 427, 699
LmnI GCTCC 1 cut(s) 81
LpnPI CCDG 8 cut(s) 144, 236, 419, 452, 458, 485, 543, 650
Lsp1109I GCAGC 5 cut(s) 125, 358, 367, 368, 371
LweI GCATC 2 cut(s) 129, 594
MaeI CTAG 2 cut(s) 624, 723
MalI GATC 4 cut(s) 10, 20, 429, 701
MboI GATC 4 cut(s) 8, 18, 427, 699
MboII GAAGA 4 cut(s) 197, 254, 504, 684
MhlI GDGCHC 1 cut(s) 106
MluCI AATT 1 cut(s) 213
MlyI GAGTC 1 cut(s) 74
MmeI TCCRAC 1 cut(s) 550
MnlI CCTC 9 cut(s) 72, 202, 295, 336, 383, 446, 576, 618, 687
MseI TTAA 3 cut(s) 270, 309, 420
MslI CAYNNNNRTG 1 cut(s) 324
MspA1I CMGCKG 2 cut(s) 433, 557
MspCI CTTAAG 1 cut(s) 419
MspI CCGG 2 cut(s) 439, 727
MspR9I CCNGG 3 cut(s) 439, 440, 473
Mva1269I GAATGC 1 cut(s) 592
MvaI CCWGG 1 cut(s) 473
MvnI CGCG 1 cut(s) 44
MwoI GCNNNNNNNGC 7 cut(s) 101, 110, 164, 368, 377, 380, 563
NciI CCSGG 2 cut(s) 439, 440
NdeII GATC 4 cut(s) 8, 18, 427, 699
NheI GCTAGC 1 cut(s) 722
NlaIII CATG 3 cut(s) 206, 323, 398
NlaIV GGNNCC 3 cut(s) 103, 104, 675
PctI GAATGC 1 cut(s) 592
PfeI GAWTC 3 cut(s) 173, 199, 220
PkrI GCNGC 5 cut(s) 115, 358, 361, 373, 382
PleI GAGTC 1 cut(s) 73
PpsI GAGTC 1 cut(s) 73
Psp6I CCWGG 1 cut(s) 471
PspGI CCWGG 1 cut(s) 471
PspN4I GGNNCC 3 cut(s) 103, 104, 675
PspOMI GGGCCC 1 cut(s) 102
PspPI GGNCC 3 cut(s) 102, 103, 436
PvuII CAGCTG 2 cut(s) 433, 557
RsaI GTAC 2 cut(s) 352, 628
RsaNI GTAC 2 cut(s) 351, 627
RseI CAYNNNNRTG 1 cut(s) 324
SaqAI TTAA 3 cut(s) 270, 309, 420
SatI GCNGC 5 cut(s) 114, 357, 360, 372, 381
Sau3AI GATC 4 cut(s) 8, 18, 427, 699
Sau96I GGNCC 3 cut(s) 102, 103, 436
SchI GAGTC 1 cut(s) 74
ScrFI CCNGG 3 cut(s) 439, 440, 473
SduI GDGCHC 1 cut(s) 106
SfaNI GCATC 2 cut(s) 129, 594
SfcI CTRYAG 1 cut(s) 552
SmaI CCCGGG 1 cut(s) 440
SmiMI CAYNNNNRTG 1 cut(s) 324
SmlI CTYRAG 1 cut(s) 419
SmoI CTYRAG 1 cut(s) 419
Sse9I AATT 1 cut(s) 213
SsiI CCGC 5 cut(s) 42, 195, 425, 518, 531
SspMI CTAG 2 cut(s) 624, 723
StyD4I CCNGG 3 cut(s) 437, 438, 471
TaqI TCGA 2 cut(s) 11, 451
TasI AATT 1 cut(s) 213
TatI WGTACW 1 cut(s) 350
TfiI GAWTC 3 cut(s) 173, 199, 220
Tru1I TTAA 3 cut(s) 270, 309, 420
Tru9I TTAA 3 cut(s) 270, 309, 420
TscAI CASTG 1 cut(s) 724
TseI GCWGC 5 cut(s) 113, 356, 359, 371, 380
TspDTI ATGAA 3 cut(s) 288, 515, 728
TspMI CCCGGG 1 cut(s) 438
TspRI CASTG 1 cut(s) 724
Vha464I CTTAAG 1 cut(s) 419
XmaI CCCGGG 1 cut(s) 438
XmiI GTMKAC 1 cut(s) 551
XspI CTAG 2 cut(s) 624, 723
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.