RLG00000030378

Transcription initiation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0001612
Physical Location & Seq
Reverse (-)
60341946 .. 60365800
23855 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030378

Sequence Viewer

Length: 993 bp
ATGGGGTTTTCACCATTCTTCCCAAACCCTCTGTGTCCTCCTGTCAAAAATCCTAACAAGCGTAGTGCTTCTCCGCCACCACAGCCATCAAGTAAGAAGCAAAAGGTAGCTGATGATGAGATCGACACAGATCAAGAAGGCATTGAGCAACTCCGCGACGTCATTTCTGTCAGTGGAGTCAATGTTGTGGAAGAGCAAGAGCGCATGCTGTTGCTTTCGGGGCCCAAGAAGCAGCAAGCATCTCGAAAACTTGTGTTTCAAGAAAACCAAGAAGGGCAGGTTTTGCAGAAGATTCCCCTGCAGAAAAAGTTGGCGAGAATCATGGGGAGGTGTGGTTTGAGAATTGGTGTTGACAAAGATGTGGAGATATTCTTGTCAATGTGTGTGGAGGAGAGAATGAGGGGGATCATATGCAATTTGATTAGACAGTCGAAGCAGTGGGTTGATTGCAAAAAATCGAGACACCACACTGTTTCTGTATCTGATGTGAAGCAAGAGATTATGATTCTCAACAGGAAAGCTACGGAAGAGTTGCTGGAAAAGGCTCAAAAGCTTAATGAAGCTGAGGTTAACACTAACGGATTTGAGGGTGTTCAGGACAAACATGATCATGGAGATGAAAAAATGAGGAGAAGTACAGCAGCAGCAAATGTTGCTGCCCTTGCTGCTCTTGGAGGACATGATATATTGGCAAAATGGCAACTTAAGCGTCGAGGTAAAGATGTAAACCACAAGGCTACATCAACTGCTGGAAGAACAATGAATGGCAATGTAGAAGCAGAGAAAAGGGGCAGTGTAGAAGCTTTGGTGTCTGCAGCTGGGAGTGCTGTTGGAAAGTTTGGGAGGAATGCCCAAGTTATGATGATGCCTCAAACAAAAGTGGCTAGTACTCGTAGTATACCTGTCAAGGATGTGATTGCTGTTCTCGAAAAGGAACCTCAGATGTCCAAGTCTACTCTGATCTATTTACTAATGAAAACTGCTAGCCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000428 GO:0001650 GO:0003674 GO:0003676 GO:0003677 GO:0003712 GO:0003713 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005667 GO:0005669 GO:0005730 GO:0005911 GO:0006139 GO:0006351 GO:0006352 GO:0006355 GO:0006357 GO:0006366 GO:0006367 GO:0006725 GO:0006807 GO:0008134 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009506 GO:0009889 GO:0009891 GO:0009893 GO:0009966 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0010646 GO:0016070 GO:0016591 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0023051 GO:0030054 GO:0030880 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031974 GO:0031981 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044452 GO:0044464 GO:0044798 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048522 GO:0048583 GO:0050789 GO:0050794 GO:0051059 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0055029 GO:0055044 GO:0060255 GO:0061695 GO:0065007 GO:0070013 GO:0071704 GO:0080090 GO:0090304 GO:0090575 GO:0097159 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1901796 GO:1902494 GO:1902531 GO:1902680 GO:1903506 GO:1903508 GO:1990234 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03022 ko05016 ko05168 map03022 map05016 map05168
Pfam Domains
Protein Families
RST TAF4

Protein Analysis

331

Amino Acids

36.56

Weight (kDa)

9.57

Isoelectric Point (pI)

50.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TAF4 PF05236 51 - 322 1.7e-43 Transcription initiation factor TFIID component TAF4 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 162
Acc36I ACCTGC 1 cut(s) 268
AccI GTMKAC 2 cut(s) 898, 953
AccII CGCG 1 cut(s) 156
AciI CCGC 2 cut(s) 74, 154
AclWI GGATC 1 cut(s) 413
AcyI GRCGYC 1 cut(s) 159
AfaI GTAC 2 cut(s) 637, 889
AflII CTTAAG 1 cut(s) 704
AgsI TTSAA 1 cut(s) 260
AluBI AGCT 6 cut(s) 110, 521, 553, 563, 803, 818
AluI AGCT 6 cut(s) 110, 521, 553, 563, 803, 818
Alw26I GTCTC 1 cut(s) 454
AlwI GGATC 1 cut(s) 413
AoxI GGCC 1 cut(s) 221
ApaI GGGCCC 1 cut(s) 225
ApeKI GCWGC 6 cut(s) 232, 641, 644, 656, 665, 815
AspLEI GCGC 1 cut(s) 204
AspS9I GGNCC 2 cut(s) 221, 222
AsuHPI GGTGA 1 cut(s) 3
AsuNHI GCTAGC 1 cut(s) 983
BaeGI GKGCMC 1 cut(s) 225
BanII GRGCYC 1 cut(s) 225
BbvCI CCTCAGC 1 cut(s) 564
BbvI GCAGC 6 cut(s) 244, 643, 652, 653, 656, 827
BccI CCATC 1 cut(s) 94
BcgI CGANNNNNNTGC 2 cut(s) 224, 258
BclI TGATCA 1 cut(s) 607
BcoDI GTCTC 1 cut(s) 454
BfaI CTAG 2 cut(s) 885, 984
BfmI CTRYAG 2 cut(s) 299, 813
BfrI CTTAAG 1 cut(s) 704
BfuAI ACCTGC 1 cut(s) 268
BisI GCNGC 6 cut(s) 233, 642, 645, 657, 666, 816
BlsI GCNGC 6 cut(s) 234, 643, 646, 658, 667, 817
BmcAI AGTACT 1 cut(s) 889
BmgT120I GGNCC 2 cut(s) 221, 222
BmiI GGNNCC 3 cut(s) 222, 223, 936
BmsI GCATC 2 cut(s) 248, 855
BmtI GCTAGC 1 cut(s) 987
Bpu10I CCTNAGC 1 cut(s) 564
BsaHI GRCGYC 1 cut(s) 159
BsaXI ACNNNNNCTCC 4 cut(s) 168, 198, 319, 349
Bse118I RCCGGY 1 cut(s) 987
Bse3DI GCAATG 1 cut(s) 775
BseGI GGATG 1 cut(s) 916
BseMI GCAATG 1 cut(s) 775
BseMII CTCAG 2 cut(s) 555, 953
BseRI GAGGAG 2 cut(s) 404, 643
BseSI GKGCMC 1 cut(s) 225
BseXI GCAGC 6 cut(s) 244, 643, 652, 653, 656, 827
BseYI CCCAGC 1 cut(s) 818
Bsh1236I CGCG 1 cut(s) 156
BshFI GGCC 1 cut(s) 223
BsiSI CCGG 1 cut(s) 988
BsmAI GTCTC 1 cut(s) 454
BsmI GAATGC 1 cut(s) 853
BsnI GGCC 1 cut(s) 223
Bsp120I GGGCCC 1 cut(s) 221
Bsp1286I GDGCHC 1 cut(s) 225
Bsp143I GATC 5 cut(s) 120, 130, 405, 607, 960
BspACI CCGC 2 cut(s) 74, 154
BspANI GGCC 1 cut(s) 223
BspCNI CTCAG 2 cut(s) 556, 952
BspFNI CGCG 1 cut(s) 156
BspLI GGNNCC 3 cut(s) 222, 223, 936
BspMAI CTGCAG 2 cut(s) 303, 817
BspMI ACCTGC 1 cut(s) 268
BspOI GCTAGC 1 cut(s) 987
BspPI GGATC 1 cut(s) 413
BspQI GCTCTTC 1 cut(s) 186
BspTI CTTAAG 1 cut(s) 704
BsrDI GCAATG 1 cut(s) 775
BsrFI RCCGGY 1 cut(s) 987
BssAI RCCGGY 1 cut(s) 987
BssMI GATC 5 cut(s) 120, 130, 405, 607, 960
BssNAI GTATAC 1 cut(s) 899
BssNI GRCGYC 1 cut(s) 159
Bst1107I GTATAC 1 cut(s) 899
Bst4CI ACNGT 2 cut(s) 429, 472
Bst6I CTCTTC 2 cut(s) 186, 522
BstACI GRCGYC 1 cut(s) 159
BstAFI CTTAAG 1 cut(s) 704
BstAPI GCANNNNNTGC 2 cut(s) 283, 653
BstC8I GCNNGC 3 cut(s) 206, 237, 985
BstDEI CTNAG 2 cut(s) 564, 939
BstF5I GGATG 1 cut(s) 916
BstFNI CGCG 1 cut(s) 156
BstHHI GCGC 1 cut(s) 204
BstKTI GATC 5 cut(s) 123, 133, 408, 610, 963
BstMAI GTCTC 1 cut(s) 454
BstMBI GATC 5 cut(s) 120, 130, 405, 607, 960
BstMWI GCNNNNNNNGC 9 cut(s) 82, 220, 229, 283, 653, 662, 665, 706, 824
BstNSI RCATGY 1 cut(s) 208
BstSFI CTRYAG 2 cut(s) 299, 813
BstSLI GKGCMC 1 cut(s) 225
BstUI CGCG 1 cut(s) 156
BstV1I GCAGC 6 cut(s) 244, 643, 652, 653, 656, 827
BstZ17I GTATAC 1 cut(s) 899
BsuRI GGCC 1 cut(s) 223
BtsCI GGATG 1 cut(s) 916
BtsI GCAGTG 2 cut(s) 443, 799
BtsIMutI CAGTG 4 cut(s) 178, 443, 468, 799
BveI ACCTGC 1 cut(s) 268
Cac8I GCNNGC 3 cut(s) 206, 237, 985
CfoI GCGC 1 cut(s) 204
Cfr10I RCCGGY 1 cut(s) 987
Cfr13I GGNCC 2 cut(s) 221, 222
CseI GACGC 1 cut(s) 698
Csp6I GTAC 2 cut(s) 636, 888
CspCI CAANNNNNGTGG 2 cut(s) 366, 401
CviAII CATG 5 cut(s) 205, 322, 605, 611, 680
CviQI GTAC 2 cut(s) 636, 888
DdeI CTNAG 2 cut(s) 564, 939
DpnI GATC 5 cut(s) 122, 132, 407, 609, 962
DpnII GATC 5 cut(s) 120, 130, 405, 607, 960
Eam1104I CTCTTC 2 cut(s) 186, 522
EarI CTCTTC 2 cut(s) 186, 522
EciI GGCGGA 1 cut(s) 63
Eco24I GRGCYC 1 cut(s) 225
EcoO109I RGGNCCY 1 cut(s) 221
EcoT38I GRGCYC 1 cut(s) 225
FaeI CATG 5 cut(s) 208, 325, 608, 614, 683
FatI CATG 5 cut(s) 204, 321, 604, 610, 679
FauNDI CATATG 1 cut(s) 410
FbaI TGATCA 1 cut(s) 607
FblI GTMKAC 2 cut(s) 898, 953
Fnu4HI GCNGC 6 cut(s) 233, 642, 645, 657, 666, 816
FokI GGATG 1 cut(s) 923
FriOI GRGCYC 1 cut(s) 225
Fsp4HI GCNGC 6 cut(s) 233, 642, 645, 657, 666, 816
FspBI CTAG 2 cut(s) 885, 984
GlaI GCGC 1 cut(s) 203
GluI GCNGC 6 cut(s) 233, 642, 645, 657, 666, 816
GsaI CCCAGC 1 cut(s) 822
HaeIII GGCC 1 cut(s) 223
HapII CCGG 1 cut(s) 988
HgaI GACGC 1 cut(s) 698
HhaI GCGC 1 cut(s) 204
Hin1I GRCGYC 1 cut(s) 159
Hin1II CATG 5 cut(s) 208, 325, 608, 614, 683
Hin6I GCGC 1 cut(s) 202
HinP1I GCGC 1 cut(s) 202
HincII GTYRAC 2 cut(s) 352, 571
HindII GTYRAC 2 cut(s) 352, 571
HindIII AAGCTT 2 cut(s) 551, 801
HinfI GANTC 4 cut(s) 177, 292, 318, 505
HpaI GTTAAC 1 cut(s) 571
HpaII CCGG 1 cut(s) 988
HphI GGTGA 1 cut(s) 3
Hpy166II GTNNAC 5 cut(s) 352, 571, 727, 899, 954
Hpy188I TCNGA 3 cut(s) 484, 942, 960
Hpy188III TCNNGA 6 cut(s) 134, 243, 260, 459, 596, 926
Hpy8I GTNNAC 5 cut(s) 352, 571, 727, 899, 954
Hpy99I CGWCG 2 cut(s) 161, 714
HpyAV CCTTC 2 cut(s) 131, 266
HpyCH4III ACNGT 2 cut(s) 429, 472
HpyCH4IV ACGT 1 cut(s) 159
HpyCH4V TGCA 5 cut(s) 286, 301, 414, 450, 815
HpyF10VI GCNNNNNNNGC 9 cut(s) 82, 220, 229, 283, 653, 662, 665, 706, 824
HpyF3I CTNAG 2 cut(s) 564, 939
HpySE526I ACGT 1 cut(s) 159
Hsp92I GRCGYC 1 cut(s) 159
Hsp92II CATG 5 cut(s) 208, 325, 608, 614, 683
HspAI GCGC 1 cut(s) 202
Ksp22I TGATCA 1 cut(s) 607
KspAI GTTAAC 1 cut(s) 571
Kzo9I GATC 5 cut(s) 120, 130, 405, 607, 960
LguI GCTCTTC 1 cut(s) 186
LpnPI CCDG 9 cut(s) 54, 263, 311, 499, 521, 581, 735, 804, 915
Lsp1109I GCAGC 6 cut(s) 244, 643, 652, 653, 656, 827
LweI GCATC 2 cut(s) 248, 855
MaeI CTAG 2 cut(s) 885, 984
MaeII ACGT 1 cut(s) 159
MalI GATC 5 cut(s) 122, 132, 407, 609, 962
MboI GATC 5 cut(s) 120, 130, 405, 607, 960
MboII GAAGA 5 cut(s) 10, 203, 301, 539, 765
MhlI GDGCHC 1 cut(s) 225
MluCI AATT 2 cut(s) 342, 415
MlyI GAGTC 1 cut(s) 186
MmeI TCCRAC 1 cut(s) 811
MseI TTAA 3 cut(s) 555, 570, 705
MslI CAYNNNNRTG 2 cut(s) 609, 615
MspA1I CMGCKG 1 cut(s) 818
MspCI CTTAAG 1 cut(s) 704
MspI CCGG 1 cut(s) 988
Mva1269I GAATGC 1 cut(s) 853
MvnI CGCG 1 cut(s) 156
MwoI GCNNNNNNNGC 9 cut(s) 82, 220, 229, 283, 653, 662, 665, 706, 824
NdeI CATATG 1 cut(s) 410
NdeII GATC 5 cut(s) 120, 130, 405, 607, 960
NheI GCTAGC 1 cut(s) 983
NlaIII CATG 5 cut(s) 208, 325, 608, 614, 683
NlaIV GGNNCC 3 cut(s) 222, 223, 936
NspI RCATGY 1 cut(s) 208
PaeI GCATGC 1 cut(s) 208
PciSI GCTCTTC 1 cut(s) 186
PctI GAATGC 1 cut(s) 853
PfeI GAWTC 3 cut(s) 292, 318, 505
PkrI GCNGC 6 cut(s) 234, 643, 646, 658, 667, 817
PleI GAGTC 1 cut(s) 185
PpsI GAGTC 1 cut(s) 185
PspFI CCCAGC 1 cut(s) 818
PspN4I GGNNCC 3 cut(s) 222, 223, 936
PspOMI GGGCCC 1 cut(s) 221
PspPI GGNCC 2 cut(s) 221, 222
PstI CTGCAG 2 cut(s) 303, 817
PvuII CAGCTG 1 cut(s) 818
RsaI GTAC 2 cut(s) 637, 889
RsaNI GTAC 2 cut(s) 636, 888
RseI CAYNNNNRTG 2 cut(s) 609, 615
SapI GCTCTTC 1 cut(s) 186
SaqAI TTAA 3 cut(s) 555, 570, 705
SatI GCNGC 6 cut(s) 233, 642, 645, 657, 666, 816
Sau3AI GATC 5 cut(s) 120, 130, 405, 607, 960
Sau96I GGNCC 2 cut(s) 221, 222
ScaI AGTACT 1 cut(s) 889
SchI GAGTC 1 cut(s) 186
SduI GDGCHC 1 cut(s) 225
SfaNI GCATC 2 cut(s) 248, 855
SfcI CTRYAG 2 cut(s) 299, 813
SmiMI CAYNNNNRTG 2 cut(s) 609, 615
SmlI CTYRAG 1 cut(s) 704
SmoI CTYRAG 1 cut(s) 704
SphI GCATGC 1 cut(s) 208
Sse9I AATT 2 cut(s) 342, 415
SsiI CCGC 2 cut(s) 74, 154
SspMI CTAG 2 cut(s) 885, 984
TaaI ACNGT 2 cut(s) 429, 472
TaiI ACGT 1 cut(s) 162
TaqI TCGA 6 cut(s) 123, 244, 431, 458, 712, 927
TasI AATT 2 cut(s) 342, 415
TatI WGTACW 2 cut(s) 635, 887
TfiI GAWTC 3 cut(s) 292, 318, 505
Tru1I TTAA 3 cut(s) 555, 570, 705
Tru9I TTAA 3 cut(s) 555, 570, 705
TscAI CASTG 4 cut(s) 178, 443, 475, 799
TseI GCWGC 6 cut(s) 232, 641, 644, 656, 665, 815
TspDTI ATGAA 4 cut(s) 573, 633, 776, 989
TspGWI ACGGA 2 cut(s) 539, 594
TspRI CASTG 4 cut(s) 178, 443, 475, 799
Vha464I CTTAAG 1 cut(s) 704
XceI RCATGY 1 cut(s) 208
XmiI GTMKAC 2 cut(s) 898, 953
XspI CTAG 2 cut(s) 885, 984
ZraI GACGTC 1 cut(s) 160
ZrmI AGTACT 1 cut(s) 889
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.