RLG00000030380

Transcription initiation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0001612
Physical Location & Seq
Reverse (-)
60392905 .. 60395159
2255 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030380

Sequence Viewer

Length: 1173 bp
ATGGGCTCGAGGTCGGCAAAGATTACGCCTGGGATATGCTTGCCGGCACCGATCTTGGAGAAGAAGATGATGGGCTTTGACGGTGGGCTCAAGTTCGGCGAAGATTACGCCTGGGACATGCTTGCCGGCACCGATCTCGGAGAAGATGAAATCTTGGGCAGTCGATCTTCATCAATGGGGTTTTCACCATTCTTCCCAAACCCTCTGTTTCCTCCTGTCAAATACCCTAACAAGCGTAGTGCTTCTCCACCACCAAGTAAGAAGCAAAAGGTAGCTGATGATGAGATAGACACAGATCAAGAAGGCATTGAGCAACTCCGCGACGTCGTTTCTGTCAGTGGAGTCAATCTTGTGGAAGAGCAAGAGCGCATGCTGTTGCTTTCGGGGCCCAAGAAGCAGCAAGCATCTCGAAAACTTGTGCTTCAAGAAAACCAAGAAGGGCTGGTTTTGCAGAAGATTCCCCTGCAGAAAAAGTTGGCGGCAATCATGGGCAGGTGTGGTTTGAGAGTTGGTATAGATAAAGATGTGGAGAGATGCTTGTCAATGTGTGTGGAGGAGAGACTAAGGCGGATCATATGCAATTTGATTAGACAGTCGAAGCAGCGGGTTGATTGTGAAAAATCGAGACATCACACTGTTTCTACATCTGATGTGAAGCAAGAAATTATGATTCTCAACAAGAAAGCTAGGGAAAAGTTGCAGGAAAAGGCTCAGAAGCGTAATGAAGCTAAGGTTAGCAATAACGGATTTGAGGGTGTTGAGGATAAACATGACGATGAAGATGACAAAATGAGGAGAAGTACAGCAGCAGCAAATGTTGCTGCCCTTGCTGCTCTTGGAGGACATGACATATTGGCGAAATGGAAACTTAAGGCGGATCAGCTATCCCGGGAGAAGCGTCGAGGTAAAGATGTAAACCCCAAGGCTACATCAACTGTTGGAAGAACAATAGAAGCGGAGAAAAGGGGCGGTGTAGAAGCTATGGTGTCTGCAGCTGGGAGTGCTGTTGGAAAGTTTGGGAGGAATTGCCAAGTTATGATGATGCCTCAAACAAAGGTGGCTAGTACTCGTAGTATATCTGTCAAGGATGTGATTGCTGTTCTGGAAAAGGAACCTCAGACGTCCAAGTCTACTTTGATATATTTGCTAATGAACACTGCAGCCGGTGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000428 GO:0001650 GO:0003674 GO:0003676 GO:0003677 GO:0003712 GO:0003713 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005667 GO:0005669 GO:0005730 GO:0005911 GO:0006139 GO:0006351 GO:0006352 GO:0006355 GO:0006357 GO:0006366 GO:0006367 GO:0006725 GO:0006807 GO:0008134 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009506 GO:0009889 GO:0009891 GO:0009893 GO:0009966 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0010646 GO:0016070 GO:0016591 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0023051 GO:0030054 GO:0030880 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031974 GO:0031981 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044452 GO:0044464 GO:0044798 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048522 GO:0048583 GO:0050789 GO:0050794 GO:0051059 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0055029 GO:0055044 GO:0060255 GO:0061695 GO:0065007 GO:0070013 GO:0071704 GO:0080090 GO:0090304 GO:0090575 GO:0097159 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1901796 GO:1902494 GO:1902531 GO:1902680 GO:1903506 GO:1903508 GO:1990234 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03022 ko05016 ko05168 map03022 map05016 map05168
Pfam Domains
Protein Families
RST TAF4

Protein Analysis

391

Amino Acids

42.99

Weight (kDa)

9.26

Isoelectric Point (pI)

57.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TAF4 PF05236 106 - 381 6.1e-48 Transcription initiation factor TFIID component TAF4 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 483
AasI GACNNNNNNGTC 1 cut(s) 1126
AatII GACGTC 2 cut(s) 327, 1124
Acc36I ACCTGC 1 cut(s) 483
AccB1I GGYRCC 2 cut(s) 46, 128
AccI GTMKAC 1 cut(s) 1130
AccII CGCG 1 cut(s) 321
AciI CCGC 7 cut(s) 319, 479, 568, 604, 875, 956, 969
AclWI GGATC 2 cut(s) 578, 885
AcyI GRCGYC 2 cut(s) 324, 1121
AfaI GTAC 2 cut(s) 802, 1066
AflII CTTAAG 1 cut(s) 869
AgsI TTSAA 1 cut(s) 425
AjnI CCWGG 2 cut(s) 28, 110
AluBI AGCT 6 cut(s) 275, 686, 728, 883, 980, 995
AluI AGCT 6 cut(s) 275, 686, 728, 883, 980, 995
Alw26I GTCTC 2 cut(s) 553, 619
AlwI GGATC 2 cut(s) 578, 885
Ama87I CYCGRG 2 cut(s) 7, 888
AoxI GGCC 1 cut(s) 386
ApaI GGGCCC 1 cut(s) 390
ApeKI GCWGC 8 cut(s) 397, 601, 806, 809, 821, 830, 992, 1160
AspLEI GCGC 1 cut(s) 369
AspS9I GGNCC 2 cut(s) 386, 387
AsuC2I CCSGG 2 cut(s) 889, 890
AsuHPI GGTGA 1 cut(s) 177
AvaI CYCGRG 2 cut(s) 7, 888
BaeGI GKGCMC 1 cut(s) 390
BanI GGYRCC 2 cut(s) 46, 128
BanII GRGCYC 3 cut(s) 8, 90, 390
BbvI GCAGC 7 cut(s) 409, 613, 808, 817, 818, 821, 1004
BccI CCATC 1 cut(s) 64
BcgI CGANNNNNNTGC 4 cut(s) 118, 152, 389, 423
BciT130I CCWGG 2 cut(s) 30, 112
BcnI CCSGG 2 cut(s) 889, 890
BcoDI GTCTC 2 cut(s) 553, 619
BfaI CTAG 2 cut(s) 687, 1062
BfmI CTRYAG 3 cut(s) 464, 990, 1158
BfrI CTTAAG 1 cut(s) 869
BfuAI ACCTGC 1 cut(s) 483
BisI GCNGC 9 cut(s) 398, 480, 602, 807, 810, 822, 831, 993, 1161
BlsI GCNGC 9 cut(s) 399, 481, 603, 808, 811, 823, 832, 994, 1162
BmcAI AGTACT 1 cut(s) 1066
Bme1390I CCNGG 4 cut(s) 30, 112, 889, 890
BmeT110I CYCGRG 2 cut(s) 7, 888
BmgT120I GGNCC 2 cut(s) 386, 387
BmiI GGNNCC 5 cut(s) 48, 130, 387, 388, 1113
BmrFI CCNGG 4 cut(s) 30, 112, 889, 890
BmsI GCATC 3 cut(s) 413, 524, 1032
Bpu10I CCTNAGC 1 cut(s) 729
BpuEI CTTGAG 1 cut(s) 74
BpuMI CCSGG 2 cut(s) 889, 890
BsaBI GATNNNNATC 1 cut(s) 169
BsaHI GRCGYC 2 cut(s) 324, 1121
BsaJI CCNNGG 4 cut(s) 29, 111, 888, 921
Bse118I RCCGGY 3 cut(s) 43, 125, 1163
Bse8I GATNNNNATC 1 cut(s) 169
BseBI CCWGG 2 cut(s) 30, 112
BseDI CCNNGG 4 cut(s) 29, 111, 888, 921
BseGI GGATG 1 cut(s) 1093
BseJI GATNNNNATC 1 cut(s) 169
BseMII CTCAG 2 cut(s) 725, 1130
BseRI GAGGAG 2 cut(s) 569, 808
BseSI GKGCMC 1 cut(s) 390
BseXI GCAGC 7 cut(s) 409, 613, 808, 817, 818, 821, 1004
BseYI CCCAGC 1 cut(s) 995
Bsh1236I CGCG 1 cut(s) 321
BshFI GGCC 1 cut(s) 388
BshNI GGYRCC 2 cut(s) 46, 128
BsiHKCI CYCGRG 2 cut(s) 7, 888
BsiSI CCGG 4 cut(s) 44, 126, 889, 1164
BslFI GGGAC 1 cut(s) 128
BsmAI GTCTC 2 cut(s) 553, 619
BsmFI GGGAC 1 cut(s) 128
BsnI GGCC 1 cut(s) 388
BsoBI CYCGRG 2 cut(s) 7, 888
Bsp120I GGGCCC 1 cut(s) 386
Bsp1286I GDGCHC 3 cut(s) 8, 90, 390
Bsp143I GATC 6 cut(s) 51, 133, 164, 295, 570, 877
BspACI CCGC 7 cut(s) 319, 479, 568, 604, 875, 956, 969
BspANI GGCC 1 cut(s) 388
BspCNI CTCAG 2 cut(s) 724, 1129
BspFNI CGCG 1 cut(s) 321
BspLI GGNNCC 5 cut(s) 48, 130, 387, 388, 1113
BspMAI CTGCAG 3 cut(s) 468, 994, 1162
BspMI ACCTGC 1 cut(s) 483
BspPI GGATC 2 cut(s) 578, 885
BspQI GCTCTTC 1 cut(s) 351
BspT107I GGYRCC 2 cut(s) 46, 128
BspTI CTTAAG 1 cut(s) 869
BsrFI RCCGGY 3 cut(s) 43, 125, 1163
BssAI RCCGGY 3 cut(s) 43, 125, 1163
BssECI CCNNGG 4 cut(s) 29, 111, 888, 921
BssMI GATC 6 cut(s) 51, 133, 164, 295, 570, 877
BssNI GRCGYC 2 cut(s) 324, 1121
BssT1I CCWWGG 1 cut(s) 921
Bst2UI CCWGG 2 cut(s) 30, 112
Bst4CI ACNGT 4 cut(s) 83, 594, 637, 937
Bst6I CTCTTC 1 cut(s) 351
BstACI GRCGYC 2 cut(s) 324, 1121
BstAFI CTTAAG 1 cut(s) 869
BstAPI GCANNNNNTGC 1 cut(s) 818
BstC8I GCNNGC 6 cut(s) 41, 45, 123, 127, 371, 402
BstDEI CTNAG 4 cut(s) 563, 711, 729, 1116
BstF5I GGATG 1 cut(s) 1093
BstFNI CGCG 1 cut(s) 321
BstHHI GCGC 1 cut(s) 369
BstKTI GATC 6 cut(s) 54, 136, 167, 298, 573, 880
BstMAI GTCTC 2 cut(s) 553, 619
BstMBI GATC 6 cut(s) 51, 133, 164, 295, 570, 877
BstMWI GCNNNNNNNGC 7 cut(s) 385, 394, 448, 818, 827, 830, 1001
BstNI CCWGG 2 cut(s) 30, 112
BstNSI RCATGY 2 cut(s) 121, 373
BstSCI CCNGG 4 cut(s) 28, 110, 887, 888
BstSFI CTRYAG 3 cut(s) 464, 990, 1158
BstSLI GKGCMC 1 cut(s) 390
BstUI CGCG 1 cut(s) 321
BstV1I GCAGC 7 cut(s) 409, 613, 808, 817, 818, 821, 1004
BsuRI GGCC 1 cut(s) 388
BtsCI GGATG 1 cut(s) 1093
BtsI GCAGTG 1 cut(s) 1155
BtsIMutI CAGTG 3 cut(s) 343, 633, 1155
BveI ACCTGC 1 cut(s) 483
Cac8I GCNNGC 6 cut(s) 41, 45, 123, 127, 371, 402
CfoI GCGC 1 cut(s) 369
Cfr10I RCCGGY 3 cut(s) 43, 125, 1163
Cfr13I GGNCC 2 cut(s) 386, 387
Cfr9I CCCGGG 1 cut(s) 888
CseI GACGC 1 cut(s) 887
Csp6I GTAC 2 cut(s) 801, 1065
CspCI CAANNNNNGTGG 2 cut(s) 531, 566
CviAII CATG 5 cut(s) 118, 370, 487, 770, 845
CviQI GTAC 2 cut(s) 801, 1065
DdeI CTNAG 4 cut(s) 563, 711, 729, 1116
DpnI GATC 6 cut(s) 53, 135, 166, 297, 572, 879
DpnII GATC 6 cut(s) 51, 133, 164, 295, 570, 877
DrdI GACNNNNNNGTC 1 cut(s) 1126
DseDI GACNNNNNNGTC 1 cut(s) 1126
Eam1104I CTCTTC 1 cut(s) 351
EarI CTCTTC 1 cut(s) 351
EciI GGCGGA 2 cut(s) 583, 890
Eco130I CCWWGG 1 cut(s) 921
Eco24I GRGCYC 3 cut(s) 8, 90, 390
Eco88I CYCGRG 2 cut(s) 7, 888
EcoO109I RGGNCCY 1 cut(s) 386
EcoRII CCWGG 2 cut(s) 28, 110
EcoT14I CCWWGG 1 cut(s) 921
EcoT38I GRGCYC 3 cut(s) 8, 90, 390
ErhI CCWWGG 1 cut(s) 921
FaeI CATG 5 cut(s) 121, 373, 490, 773, 848
FaqI GGGAC 1 cut(s) 128
FatI CATG 5 cut(s) 117, 369, 486, 769, 844
FauI CCCGC 1 cut(s) 597
FauNDI CATATG 1 cut(s) 575
FblI GTMKAC 1 cut(s) 1130
Fnu4HI GCNGC 9 cut(s) 398, 480, 602, 807, 810, 822, 831, 993, 1161
FokI GGATG 1 cut(s) 1100
FriOI GRGCYC 3 cut(s) 8, 90, 390
Fsp4HI GCNGC 9 cut(s) 398, 480, 602, 807, 810, 822, 831, 993, 1161
FspBI CTAG 2 cut(s) 687, 1062
GlaI GCGC 1 cut(s) 368
GluI GCNGC 9 cut(s) 398, 480, 602, 807, 810, 822, 831, 993, 1161
GsaI CCCAGC 1 cut(s) 999
HaeIII GGCC 1 cut(s) 388
HapII CCGG 4 cut(s) 44, 126, 889, 1164
HgaI GACGC 1 cut(s) 887
HhaI GCGC 1 cut(s) 369
Hin1I GRCGYC 2 cut(s) 324, 1121
Hin1II CATG 5 cut(s) 121, 373, 490, 773, 848
Hin6I GCGC 1 cut(s) 367
HinP1I GCGC 1 cut(s) 367
HinfI GANTC 3 cut(s) 342, 457, 670
HpaII CCGG 4 cut(s) 44, 126, 889, 1164
HphI GGTGA 1 cut(s) 177
Hpy166II GTNNAC 2 cut(s) 916, 1131
Hpy188I TCNGA 4 cut(s) 140, 649, 714, 1119
Hpy188III TCNNGA 5 cut(s) 299, 408, 425, 624, 1103
Hpy8I GTNNAC 2 cut(s) 916, 1131
Hpy99I CGWCG 3 cut(s) 326, 329, 903
HpyAV CCTTC 2 cut(s) 296, 431
HpyCH4III ACNGT 4 cut(s) 83, 594, 637, 937
HpyCH4IV ACGT 2 cut(s) 324, 1121
HpyCH4V TGCA 6 cut(s) 451, 466, 579, 700, 992, 1160
HpyF10VI GCNNNNNNNGC 7 cut(s) 385, 394, 448, 818, 827, 830, 1001
HpyF3I CTNAG 4 cut(s) 563, 711, 729, 1116
HpySE526I ACGT 2 cut(s) 324, 1121
Hsp92I GRCGYC 2 cut(s) 324, 1121
Hsp92II CATG 5 cut(s) 121, 373, 490, 773, 848
HspAI GCGC 1 cut(s) 367
KroI GCCGGC 2 cut(s) 43, 125
KroNI GCCGGC 2 cut(s) 45, 127
Kzo9I GATC 6 cut(s) 51, 133, 164, 295, 570, 877
LguI GCTCTTC 1 cut(s) 351
Lsp1109I GCAGC 7 cut(s) 409, 613, 808, 817, 818, 821, 1004
LweI GCATC 3 cut(s) 413, 524, 1032
MaeI CTAG 2 cut(s) 687, 1062
MaeII ACGT 2 cut(s) 324, 1121
MalI GATC 6 cut(s) 53, 135, 166, 297, 572, 879
MboI GATC 6 cut(s) 51, 133, 164, 295, 570, 877
MhlI GDGCHC 3 cut(s) 8, 90, 390
MluCI AATT 3 cut(s) 580, 663, 1024
MlyI GAGTC 1 cut(s) 351
MmeI TCCRAC 2 cut(s) 919, 988
MroNI GCCGGC 2 cut(s) 43, 125
MseI TTAA 1 cut(s) 870
MslI CAYNNNNRTG 1 cut(s) 774
MspA1I CMGCKG 2 cut(s) 604, 995
MspCI CTTAAG 1 cut(s) 869
MspI CCGG 4 cut(s) 44, 126, 889, 1164
MspR9I CCNGG 4 cut(s) 30, 112, 889, 890
MvaI CCWGG 2 cut(s) 30, 112
MvnI CGCG 1 cut(s) 321
MwoI GCNNNNNNNGC 7 cut(s) 385, 394, 448, 818, 827, 830, 1001
NaeI GCCGGC 2 cut(s) 45, 127
NciI CCSGG 2 cut(s) 889, 890
NdeI CATATG 1 cut(s) 575
NdeII GATC 6 cut(s) 51, 133, 164, 295, 570, 877
NgoMIV GCCGGC 2 cut(s) 43, 125
NlaIII CATG 5 cut(s) 121, 373, 490, 773, 848
NlaIV GGNNCC 5 cut(s) 48, 130, 387, 388, 1113
NspI RCATGY 2 cut(s) 121, 373
PaeI GCATGC 1 cut(s) 373
PaeR7I CTCGAG 1 cut(s) 7
PaqCI CACCTGC 1 cut(s) 483
PciSI GCTCTTC 1 cut(s) 351
PdiI GCCGGC 2 cut(s) 45, 127
PfeI GAWTC 2 cut(s) 457, 670
PkrI GCNGC 9 cut(s) 399, 481, 603, 808, 811, 823, 832, 994, 1162
PleI GAGTC 1 cut(s) 350
PpsI GAGTC 1 cut(s) 350
Psp6I CCWGG 2 cut(s) 28, 110
PspFI CCCAGC 1 cut(s) 995
PspGI CCWGG 2 cut(s) 28, 110
PspN4I GGNNCC 5 cut(s) 48, 130, 387, 388, 1113
PspOMI GGGCCC 1 cut(s) 386
PspPI GGNCC 2 cut(s) 386, 387
PspXI VCTCGAGB 1 cut(s) 7
PstI CTGCAG 3 cut(s) 468, 994, 1162
PvuII CAGCTG 1 cut(s) 995
RsaI GTAC 2 cut(s) 802, 1066
RsaNI GTAC 2 cut(s) 801, 1065
RseI CAYNNNNRTG 1 cut(s) 774
SapI GCTCTTC 1 cut(s) 351
SaqAI TTAA 1 cut(s) 870
SatI GCNGC 9 cut(s) 398, 480, 602, 807, 810, 822, 831, 993, 1161
Sau3AI GATC 6 cut(s) 51, 133, 164, 295, 570, 877
Sau96I GGNCC 2 cut(s) 386, 387
ScaI AGTACT 1 cut(s) 1066
SchI GAGTC 1 cut(s) 351
ScrFI CCNGG 4 cut(s) 30, 112, 889, 890
SduI GDGCHC 3 cut(s) 8, 90, 390
SfaNI GCATC 3 cut(s) 413, 524, 1032
SfcI CTRYAG 3 cut(s) 464, 990, 1158
Sfr274I CTCGAG 1 cut(s) 7
SlaI CTCGAG 1 cut(s) 7
SmaI CCCGGG 1 cut(s) 890
SmiMI CAYNNNNRTG 1 cut(s) 774
SmlI CTYRAG 3 cut(s) 7, 89, 869
SmoI CTYRAG 3 cut(s) 7, 89, 869
SphI GCATGC 1 cut(s) 373
Sse9I AATT 3 cut(s) 580, 663, 1024
SsiI CCGC 7 cut(s) 319, 479, 568, 604, 875, 956, 969
SspMI CTAG 2 cut(s) 687, 1062
StyD4I CCNGG 4 cut(s) 28, 110, 887, 888
StyI CCWWGG 1 cut(s) 921
TaaI ACNGT 4 cut(s) 83, 594, 637, 937
TaiI ACGT 2 cut(s) 327, 1124
TaqI TCGA 6 cut(s) 8, 163, 409, 596, 623, 901
TasI AATT 3 cut(s) 580, 663, 1024
TatI WGTACW 2 cut(s) 800, 1064
TauI GCSGC 1 cut(s) 482
TfiI GAWTC 2 cut(s) 457, 670
Tru1I TTAA 1 cut(s) 870
Tru9I TTAA 1 cut(s) 870
TscAI CASTG 3 cut(s) 343, 640, 1162
TseI GCWGC 8 cut(s) 397, 601, 806, 809, 821, 830, 992, 1160
TspDTI ATGAA 5 cut(s) 159, 162, 738, 792, 1166
TspGWI ACGGA 1 cut(s) 759
TspMI CCCGGG 1 cut(s) 888
TspRI CASTG 3 cut(s) 343, 640, 1162
Vha464I CTTAAG 1 cut(s) 869
XceI RCATGY 2 cut(s) 121, 373
XhoI CTCGAG 1 cut(s) 7
XmaI CCCGGG 1 cut(s) 888
XmiI GTMKAC 1 cut(s) 1130
XspI CTAG 2 cut(s) 687, 1062
ZraI GACGTC 2 cut(s) 325, 1122
ZrmI AGTACT 1 cut(s) 1066
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.