Rroxscaffold_4G00327130

Transcription initiation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0001612
Physical Location & Seq
Reverse (-)
59566408 .. 59567409
1002 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00327130.1

Sequence Viewer

Length: 603 bp
ATGGGGTTTTCACCATTCTTCCCAAACCCTCTGTTTCCCCTTGTCAAAAACCCTAACAAGCGTAGTGCTTCTCCACCACCAACTAAGAAGCAAAAGGTAGGCGATGATGAGATCGACACGGATCAAGAAGGCATTGAGCAACTCCGCGACGTCGTTTCTATCGGTGGAGTCAATCTTGTGGAAGAGCAAGAGCGCATGCTGTTGCTTTCGGGCCCAAGAAGCGACAAGCATCTCGAAAACTTGTGCTTCAAGAAAACCAAGAAGGGCTGGTTTTGCGGAAGATTCCCCAGAAAAAGCGGATCAGCTGGCCGGGAGAAGAGTCGAGGTAAAGATGTAAATCACAAGGCTACATCAACTGCTGGAAGAACAATGAATGGCAATGTAGAACCGGAGAAAAGCGGCGGTGTAGAAGCTTTGGTGTCTGCAGTGGAAGTGCTTGTCGGAAAGTTTGGGAGGAATTGCCAAGTTATGATGATGCCTCAAACAATGGTGGCTAGTACTCGTAGTATATCTGTCAAGGATGTGATTGTTGTTCTGGAAAAGGAGCCTCGGATGTCCAAGTTTACTCCGATCTATTTGCTAATGAACAACGCTAGCCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000428 GO:0001650 GO:0003674 GO:0003676 GO:0003677 GO:0003712 GO:0003713 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005667 GO:0005669 GO:0005730 GO:0005911 GO:0006139 GO:0006351 GO:0006352 GO:0006355 GO:0006357 GO:0006366 GO:0006367 GO:0006725 GO:0006807 GO:0008134 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009506 GO:0009889 GO:0009891 GO:0009893 GO:0009966 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0010646 GO:0016070 GO:0016591 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0023051 GO:0030054 GO:0030880 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031974 GO:0031981 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044452 GO:0044464 GO:0044798 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048522 GO:0048583 GO:0050789 GO:0050794 GO:0051059 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0055029 GO:0055044 GO:0060255 GO:0061695 GO:0065007 GO:0070013 GO:0071704 GO:0080090 GO:0090304 GO:0090575 GO:0097159 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1901796 GO:1902494 GO:1902531 GO:1902680 GO:1903506 GO:1903508 GO:1990234 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03022 ko05016 ko05168 map03022 map05016 map05168
Pfam Domains
Protein Families
RST TAF4

Protein Analysis

200

Amino Acids

22.11

Weight (kDa)

9.68

Isoelectric Point (pI)

44.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 153
AccII CGCG 1 cut(s) 147
AciI CCGC 5 cut(s) 145, 276, 297, 399, 402
AclWI GGATC 2 cut(s) 129, 307
AcoI YGGCCR 1 cut(s) 307
AcyI GRCGYC 1 cut(s) 150
AfaI GTAC 1 cut(s) 499
AgsI TTSAA 1 cut(s) 250
AluBI AGCT 2 cut(s) 305, 413
AluI AGCT 2 cut(s) 305, 413
AlwI GGATC 2 cut(s) 129, 307
AoxI GGCC 2 cut(s) 211, 307
ApaI GGGCCC 1 cut(s) 215
AspLEI GCGC 1 cut(s) 195
AspS9I GGNCC 2 cut(s) 211, 212
AsuC2I CCSGG 1 cut(s) 311
AsuHPI GGTGA 1 cut(s) 3
AsuNHI GCTAGC 1 cut(s) 593
BaeGI GKGCMC 1 cut(s) 215
BanII GRGCYC 1 cut(s) 215
BcgI CGANNNNNNTGC 2 cut(s) 559, 593
BcnI CCSGG 1 cut(s) 311
BfaI CTAG 2 cut(s) 495, 594
BfmI CTRYAG 1 cut(s) 423
BisI GCNGC 1 cut(s) 400
BlsI GCNGC 1 cut(s) 401
BmcAI AGTACT 1 cut(s) 499
Bme1390I CCNGG 1 cut(s) 311
BmgT120I GGNCC 2 cut(s) 211, 212
BmiI GGNNCC 2 cut(s) 213, 546
BmrFI CCNGG 1 cut(s) 311
BmsI GCATC 2 cut(s) 238, 465
BmtI GCTAGC 1 cut(s) 597
BpuMI CCSGG 1 cut(s) 311
BsaBI GATNNNNATC 1 cut(s) 336
BsaHI GRCGYC 1 cut(s) 150
BsaJI CCNNGG 1 cut(s) 548
BsaWI WCCGGW 1 cut(s) 388
Bse118I RCCGGY 1 cut(s) 597
Bse3DI GCAATG 1 cut(s) 385
Bse8I GATNNNNATC 1 cut(s) 336
BseDI CCNNGG 1 cut(s) 548
BseGI GGATG 2 cut(s) 526, 558
BseJI GATNNNNATC 1 cut(s) 336
BseMI GCAATG 1 cut(s) 385
BseSI GKGCMC 1 cut(s) 215
Bsh1236I CGCG 1 cut(s) 147
BshFI GGCC 2 cut(s) 213, 309
BsiSI CCGG 3 cut(s) 310, 389, 598
BsnI GGCC 2 cut(s) 213, 309
Bsp120I GGGCCC 1 cut(s) 211
Bsp1286I GDGCHC 1 cut(s) 215
Bsp143I GATC 4 cut(s) 111, 121, 299, 570
BspACI CCGC 5 cut(s) 145, 276, 297, 399, 402
BspANI GGCC 2 cut(s) 213, 309
BspFNI CGCG 1 cut(s) 147
BspLI GGNNCC 2 cut(s) 213, 546
BspMAI CTGCAG 1 cut(s) 427
BspOI GCTAGC 1 cut(s) 597
BspPI GGATC 2 cut(s) 129, 307
BspQI GCTCTTC 1 cut(s) 177
BsrDI GCAATG 1 cut(s) 385
BsrFI RCCGGY 1 cut(s) 597
BssAI RCCGGY 1 cut(s) 597
BssECI CCNNGG 1 cut(s) 548
BssMI GATC 4 cut(s) 111, 121, 299, 570
BssNI GRCGYC 1 cut(s) 150
Bst6I CTCTTC 2 cut(s) 177, 311
BstACI GRCGYC 1 cut(s) 150
BstC8I GCNNGC 3 cut(s) 197, 307, 595
BstDEI CTNAG 1 cut(s) 84
BstF5I GGATG 2 cut(s) 526, 558
BstFNI CGCG 1 cut(s) 147
BstHHI GCGC 1 cut(s) 195
BstKTI GATC 4 cut(s) 114, 124, 302, 573
BstMBI GATC 4 cut(s) 111, 121, 299, 570
BstMWI GCNNNNNNNGC 2 cut(s) 219, 273
BstNSI RCATGY 1 cut(s) 199
BstSCI CCNGG 1 cut(s) 309
BstSFI CTRYAG 1 cut(s) 423
BstSLI GKGCMC 1 cut(s) 215
BstUI CGCG 1 cut(s) 147
BsuRI GGCC 2 cut(s) 213, 309
BtgZI GCGATG 1 cut(s) 117
BtsCI GGATG 2 cut(s) 526, 558
BtsI GCAGTG 1 cut(s) 432
BtsIMutI CAGTG 1 cut(s) 432
Cac8I GCNNGC 3 cut(s) 197, 307, 595
CfoI GCGC 1 cut(s) 195
Cfr10I RCCGGY 1 cut(s) 597
Cfr13I GGNCC 2 cut(s) 211, 212
Csp6I GTAC 1 cut(s) 498
CviAII CATG 1 cut(s) 196
CviJI RGCY 9 cut(s) 213, 267, 305, 309, 347, 413, 494, 547, 597
CviKI_1 RGCY 9 cut(s) 213, 267, 305, 309, 347, 413, 494, 547, 597
CviQI GTAC 1 cut(s) 498
DdeI CTNAG 1 cut(s) 84
DpnI GATC 4 cut(s) 113, 123, 301, 572
DpnII GATC 4 cut(s) 111, 121, 299, 570
EaeI YGGCCR 1 cut(s) 307
Eam1104I CTCTTC 2 cut(s) 177, 311
EarI CTCTTC 2 cut(s) 177, 311
Eco24I GRGCYC 1 cut(s) 215
EcoT38I GRGCYC 1 cut(s) 215
FaeI CATG 1 cut(s) 199
FaiI YATR 3 cut(s) 197, 470, 509
FatI CATG 1 cut(s) 195
Fnu4HI GCNGC 1 cut(s) 400
FokI GGATG 2 cut(s) 533, 565
FriOI GRGCYC 1 cut(s) 215
Fsp4HI GCNGC 1 cut(s) 400
FspBI CTAG 2 cut(s) 495, 594
GlaI GCGC 1 cut(s) 194
GluI GCNGC 1 cut(s) 400
HaeIII GGCC 2 cut(s) 213, 309
HapII CCGG 3 cut(s) 310, 389, 598
HhaI GCGC 1 cut(s) 195
Hin1I GRCGYC 1 cut(s) 150
Hin1II CATG 1 cut(s) 199
Hin6I GCGC 1 cut(s) 193
HinP1I GCGC 1 cut(s) 193
HindIII AAGCTT 1 cut(s) 411
HinfI GANTC 3 cut(s) 168, 282, 319
HpaII CCGG 3 cut(s) 310, 389, 598
HphI GGTGA 1 cut(s) 3
Hpy166II GTNNAC 1 cut(s) 564
Hpy188I TCNGA 3 cut(s) 443, 552, 570
Hpy188III TCNNGA 4 cut(s) 125, 233, 250, 536
Hpy8I GTNNAC 1 cut(s) 564
Hpy99I CGWCG 2 cut(s) 152, 155
HpyAV CCTTC 2 cut(s) 122, 256
HpyCH4IV ACGT 1 cut(s) 150
HpyCH4V TGCA 1 cut(s) 425
HpyF10VI GCNNNNNNNGC 2 cut(s) 219, 273
HpyF3I CTNAG 1 cut(s) 84
HpySE526I ACGT 1 cut(s) 150
Hsp92I GRCGYC 1 cut(s) 150
Hsp92II CATG 1 cut(s) 199
HspAI GCGC 1 cut(s) 193
Kzo9I GATC 4 cut(s) 111, 121, 299, 570
LguI GCTCTTC 1 cut(s) 177
LmnI GCTCC 1 cut(s) 544
LpnPI CCDG 7 cut(s) 253, 291, 301, 323, 345, 402, 521
LweI GCATC 2 cut(s) 238, 465
MaeI CTAG 2 cut(s) 495, 594
MaeII ACGT 1 cut(s) 150
MalI GATC 4 cut(s) 113, 123, 301, 572
MboI GATC 4 cut(s) 111, 121, 299, 570
MboII GAAGA 5 cut(s) 10, 194, 291, 328, 375
MhlI GDGCHC 1 cut(s) 215
MluCI AATT 1 cut(s) 457
MlyI GAGTC 2 cut(s) 177, 328
MmeI TCCRAC 1 cut(s) 421
MnlI CCTC 5 cut(s) 39, 317, 447, 489, 558
MspA1I CMGCKG 1 cut(s) 305
MspI CCGG 3 cut(s) 310, 389, 598
MspR9I CCNGG 1 cut(s) 311
MvnI CGCG 1 cut(s) 147
MwoI GCNNNNNNNGC 2 cut(s) 219, 273
NciI CCSGG 1 cut(s) 311
NdeII GATC 4 cut(s) 111, 121, 299, 570
NheI GCTAGC 1 cut(s) 593
NlaIII CATG 1 cut(s) 199
NlaIV GGNNCC 2 cut(s) 213, 546
NspI RCATGY 1 cut(s) 199
PaeI GCATGC 1 cut(s) 199
PciSI GCTCTTC 1 cut(s) 177
PfeI GAWTC 1 cut(s) 282
PkrI GCNGC 1 cut(s) 401
PleI GAGTC 2 cut(s) 176, 327
PpsI GAGTC 2 cut(s) 176, 327
PspN4I GGNNCC 2 cut(s) 213, 546
PspOMI GGGCCC 1 cut(s) 211
PspPI GGNCC 2 cut(s) 211, 212
PstI CTGCAG 1 cut(s) 427
PvuII CAGCTG 1 cut(s) 305
RsaI GTAC 1 cut(s) 499
RsaNI GTAC 1 cut(s) 498
SapI GCTCTTC 1 cut(s) 177
SatI GCNGC 1 cut(s) 400
Sau3AI GATC 4 cut(s) 111, 121, 299, 570
Sau96I GGNCC 2 cut(s) 211, 212
ScaI AGTACT 1 cut(s) 499
SchI GAGTC 2 cut(s) 177, 328
ScrFI CCNGG 1 cut(s) 311
SduI GDGCHC 1 cut(s) 215
SetI ASST 5 cut(s) 99, 153, 307, 328, 415
SfaNI GCATC 2 cut(s) 238, 465
SfcI CTRYAG 1 cut(s) 423
SphI GCATGC 1 cut(s) 199
Sse9I AATT 1 cut(s) 457
SsiI CCGC 5 cut(s) 145, 276, 297, 399, 402
SspMI CTAG 2 cut(s) 495, 594
StyD4I CCNGG 1 cut(s) 309
TaiI ACGT 1 cut(s) 153
TaqI TCGA 3 cut(s) 114, 234, 322
TasI AATT 1 cut(s) 457
TatI WGTACW 1 cut(s) 497
TauI GCSGC 1 cut(s) 402
TfiI GAWTC 1 cut(s) 282
TscAI CASTG 1 cut(s) 432
TspDTI ATGAA 2 cut(s) 386, 599
TspGWI ACGGA 1 cut(s) 134
TspRI CASTG 1 cut(s) 432
XceI RCATGY 1 cut(s) 199
XspI CTAG 2 cut(s) 495, 594
ZraI GACGTC 1 cut(s) 151
ZrmI AGTACT 1 cut(s) 499
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.