Rmu_sc0000749.1_g000030

Transcription initiation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000749.1
OG0001612
Physical Location & Seq
Reverse (-)
104477 .. 105484
1008 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000749.1_g000030.1.cds

Sequence Viewer

Length: 1008 bp
atggggttttcaccattcttcccaaaccctctgtttcctcctgtcaaaaatcctaacaagcgtcgtgcttcaccgccaccaagtaagaagcaaaaggtagctgatgatgagatcgacacagatcaagaaggcattgagcaactccgcgacgtcgtttctgtcagtggagtcaatcttgtggaagagcaagagcgcatgctgttgctttcggggcccaagaatcagcaagcatctcgaaaacttgtgtttcaagaaaaccaagaagggcaggttttgcagaagattcccctgcagaaaaagttggcagaagtgatggggaggtgcggtttgagaattggtgtagacaaagatgtggagagatgcttgtcaatgtgtgtggaggagagaatgagggggatcatttgcaatttgataagacagtcgaagcagcgggttgattgcgaaaaattgagacaccacactgtttctacatctgatgtgaagcaagagattatgattctcaacaggaaagctggggaagagttgctggaaaatgctcagaagcttaatgaagctaaggttagcactaacggatttgagggtgttaaggataaacatgatgatgcagatgacagaatgaggagaagtacagcagcagcaaatgttgctgcccttgctgctcttggaggacatgacatattggcgaaatggaaacttaaggcggatcagctatcgcgggagaagcgtcgaggtaaagatgtaaacccccaggctacatcaactgctggaagaacaatgaatggcaatgtagaagcggagaaaaggggcggtgtagaagctttggtgtctgcagctgggagtgctgttggaaagtatgggaggaatgcccaagttatgatgatgcctcaaacaaaggtggctagtactggtagtatatctgtcaaggatgtgattgctgttctggaaaaggaacctcaggtgtccaagtctactctgatatatttgctaatgaacactgctagccggtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000428 GO:0001650 GO:0003674 GO:0003676 GO:0003677 GO:0003712 GO:0003713 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005667 GO:0005669 GO:0005730 GO:0005911 GO:0006139 GO:0006351 GO:0006352 GO:0006355 GO:0006357 GO:0006366 GO:0006367 GO:0006725 GO:0006807 GO:0008134 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009506 GO:0009889 GO:0009891 GO:0009893 GO:0009966 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0010646 GO:0016070 GO:0016591 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0023051 GO:0030054 GO:0030880 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031974 GO:0031981 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044452 GO:0044464 GO:0044798 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048522 GO:0048583 GO:0050789 GO:0050794 GO:0051059 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0055029 GO:0055044 GO:0060255 GO:0061695 GO:0065007 GO:0070013 GO:0071704 GO:0080090 GO:0090304 GO:0090575 GO:0097159 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1901796 GO:1902494 GO:1902531 GO:1902680 GO:1903506 GO:1903508 GO:1990234 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03022 ko05016 ko05168 map03022 map05016 map05168
Pfam Domains
Protein Families
RST TAF4

Protein Analysis

335

Amino Acids

36.94

Weight (kDa)

9.4

Isoelectric Point (pI)

40.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Style Settings
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 153
Acc36I ACCTGC 1 cut(s) 259
AccI GTMKAC 2 cut(s) 342, 968
AccII CGCG 2 cut(s) 147, 715
AciI CCGC 8 cut(s) 74, 145, 324, 430, 701, 715, 794, 807
AclWI GGATC 2 cut(s) 404, 711
AcyI GRCGYC 1 cut(s) 150
AfaI GTAC 2 cut(s) 628, 904
AflII CTTAAG 1 cut(s) 695
AgsI TTSAA 1 cut(s) 251
AjnI CCWGG 1 cut(s) 747
AluBI AGCT 7 cut(s) 101, 512, 544, 554, 709, 818, 833
AluI AGCT 7 cut(s) 101, 512, 544, 554, 709, 818, 833
Alw26I GTCTC 1 cut(s) 445
AlwI GGATC 2 cut(s) 404, 711
AoxI GGCC 1 cut(s) 212
ApaI GGGCCC 1 cut(s) 216
ApeKI GCWGC 6 cut(s) 427, 632, 635, 647, 656, 830
AspLEI GCGC 1 cut(s) 195
AspS9I GGNCC 2 cut(s) 212, 213
AsuHPI GGTGA 2 cut(s) 3, 63
AsuNHI GCTAGC 1 cut(s) 998
AxyI CCTNAGG 1 cut(s) 954
BaeGI GKGCMC 1 cut(s) 216
BanII GRGCYC 1 cut(s) 216
BbvI GCAGC 6 cut(s) 439, 634, 643, 644, 647, 842
BccI CCATC 1 cut(s) 307
BcgI CGANNNNNNTGC 2 cut(s) 215, 249
BciT130I CCWGG 1 cut(s) 749
BcoDI GTCTC 1 cut(s) 445
BfaI CTAG 2 cut(s) 900, 999
BfmI CTRYAG 2 cut(s) 290, 828
BfrI CTTAAG 1 cut(s) 695
BfuAI ACCTGC 1 cut(s) 259
BisI GCNGC 6 cut(s) 428, 633, 636, 648, 657, 831
BlsI GCNGC 6 cut(s) 429, 634, 637, 649, 658, 832
BmcAI AGTACT 1 cut(s) 904
Bme1390I CCNGG 1 cut(s) 749
BmgT120I GGNCC 2 cut(s) 212, 213
BmiI GGNNCC 3 cut(s) 213, 214, 951
BmrFI CCNGG 1 cut(s) 749
BmsI GCATC 4 cut(s) 239, 350, 592, 870
BmtI GCTAGC 1 cut(s) 1002
Bpu10I CCTNAGC 1 cut(s) 555
BsaHI GRCGYC 1 cut(s) 150
BsaJI CCNNGG 1 cut(s) 747
BsaXI ACNNNNNCTCC 2 cut(s) 310, 340
Bse118I RCCGGY 1 cut(s) 1002
Bse1I ACTGG 1 cut(s) 910
Bse21I CCTNAGG 1 cut(s) 954
Bse3DI GCAATG 1 cut(s) 790
BseBI CCWGG 1 cut(s) 749
BseDI CCNNGG 1 cut(s) 747
BseGI GGATG 1 cut(s) 931
BseMI GCAATG 1 cut(s) 790
BseMII CTCAG 2 cut(s) 551, 968
BseNI ACTGG 1 cut(s) 910
BseRI GAGGAG 2 cut(s) 395, 634
BseSI GKGCMC 1 cut(s) 216
BseXI GCAGC 6 cut(s) 439, 634, 643, 644, 647, 842
BseYI CCCAGC 2 cut(s) 512, 833
Bsh1236I CGCG 2 cut(s) 147, 715
BshFI GGCC 1 cut(s) 214
BsiSI CCGG 1 cut(s) 1003
BsmAI GTCTC 1 cut(s) 445
BsmI GAATGC 1 cut(s) 868
BsnI GGCC 1 cut(s) 214
Bsp120I GGGCCC 1 cut(s) 212
Bsp1286I GDGCHC 1 cut(s) 216
Bsp143I GATC 4 cut(s) 111, 121, 396, 703
BspACI CCGC 8 cut(s) 74, 145, 324, 430, 701, 715, 794, 807
BspANI GGCC 1 cut(s) 214
BspCNI CTCAG 2 cut(s) 550, 967
BspFNI CGCG 2 cut(s) 147, 715
BspLI GGNNCC 3 cut(s) 213, 214, 951
BspMAI CTGCAG 2 cut(s) 294, 832
BspMI ACCTGC 1 cut(s) 259
BspOI GCTAGC 1 cut(s) 1002
BspPI GGATC 2 cut(s) 404, 711
BspQI GCTCTTC 1 cut(s) 177
BspTI CTTAAG 1 cut(s) 695
BsrDI GCAATG 1 cut(s) 790
BsrFI RCCGGY 1 cut(s) 1002
BsrI ACTGG 1 cut(s) 910
BssAI RCCGGY 1 cut(s) 1002
BssECI CCNNGG 1 cut(s) 747
BssMI GATC 4 cut(s) 111, 121, 396, 703
BssNI GRCGYC 1 cut(s) 150
Bst2UI CCWGG 1 cut(s) 749
Bst4CI ACNGT 2 cut(s) 420, 463
Bst6I CTCTTC 2 cut(s) 177, 513
BstACI GRCGYC 1 cut(s) 150
BstAFI CTTAAG 1 cut(s) 695
BstAPI GCANNNNNTGC 2 cut(s) 274, 644
BstC8I GCNNGC 3 cut(s) 197, 228, 1000
BstDEI CTNAG 3 cut(s) 537, 555, 954
BstF5I GGATG 1 cut(s) 931
BstFNI CGCG 2 cut(s) 147, 715
BstHHI GCGC 1 cut(s) 195
BstKTI GATC 4 cut(s) 114, 124, 399, 706
BstMAI GTCTC 1 cut(s) 445
BstMBI GATC 4 cut(s) 111, 121, 396, 703
BstMWI GCNNNNNNNGC 7 cut(s) 211, 274, 644, 653, 656, 721, 839
BstNI CCWGG 1 cut(s) 749
BstNSI RCATGY 1 cut(s) 199
BstSCI CCNGG 1 cut(s) 747
BstSFI CTRYAG 2 cut(s) 290, 828
BstSLI GKGCMC 1 cut(s) 216
BstUI CGCG 2 cut(s) 147, 715
BstV1I GCAGC 6 cut(s) 439, 634, 643, 644, 647, 842
Bsu36I CCTNAGG 1 cut(s) 954
BsuRI GGCC 1 cut(s) 214
BtsCI GGATG 1 cut(s) 931
BtsI GCAGTG 1 cut(s) 993
BtsIMutI CAGTG 3 cut(s) 169, 459, 993
BveI ACCTGC 1 cut(s) 259
Cac8I GCNNGC 3 cut(s) 197, 228, 1000
CfoI GCGC 1 cut(s) 195
Cfr10I RCCGGY 1 cut(s) 1002
Cfr13I GGNCC 2 cut(s) 212, 213
CseI GACGC 2 cut(s) 50, 713
Csp6I GTAC 2 cut(s) 627, 903
CspCI CAANNNNNGTGG 2 cut(s) 357, 392
CviAII CATG 3 cut(s) 196, 596, 671
CviQI GTAC 2 cut(s) 627, 903
DdeI CTNAG 3 cut(s) 537, 555, 954
DpnI GATC 4 cut(s) 113, 123, 398, 705
DpnII GATC 4 cut(s) 111, 121, 396, 703
Eam1104I CTCTTC 2 cut(s) 177, 513
EarI CTCTTC 2 cut(s) 177, 513
EciI GGCGGA 1 cut(s) 716
Eco24I GRGCYC 1 cut(s) 216
Eco81I CCTNAGG 1 cut(s) 954
EcoO109I RGGNCCY 1 cut(s) 212
EcoRII CCWGG 1 cut(s) 747
EcoT38I GRGCYC 1 cut(s) 216
FaeI CATG 3 cut(s) 199, 599, 674
FaiI YATR 9 cut(s) 197, 494, 597, 672, 677, 855, 875, 914, 979
FatI CATG 3 cut(s) 195, 595, 670
FauI CCCGC 2 cut(s) 423, 708
FblI GTMKAC 2 cut(s) 342, 968
Fnu4HI GCNGC 6 cut(s) 428, 633, 636, 648, 657, 831
FokI GGATG 1 cut(s) 938
FriOI GRGCYC 1 cut(s) 216
Fsp4HI GCNGC 6 cut(s) 428, 633, 636, 648, 657, 831
FspBI CTAG 2 cut(s) 900, 999
GlaI GCGC 1 cut(s) 194
GluI GCNGC 6 cut(s) 428, 633, 636, 648, 657, 831
GsaI CCCAGC 2 cut(s) 516, 837
HaeIII GGCC 1 cut(s) 214
HapII CCGG 1 cut(s) 1003
HgaI GACGC 2 cut(s) 50, 713
HhaI GCGC 1 cut(s) 195
Hin1I GRCGYC 1 cut(s) 150
Hin1II CATG 3 cut(s) 199, 599, 674
Hin6I GCGC 1 cut(s) 193
HinP1I GCGC 1 cut(s) 193
HindIII AAGCTT 2 cut(s) 542, 816
HinfI GANTC 4 cut(s) 168, 220, 283, 496
HpaII CCGG 1 cut(s) 1003
HphI GGTGA 2 cut(s) 3, 63
Hpy166II GTNNAC 3 cut(s) 343, 742, 969
Hpy188I TCNGA 3 cut(s) 475, 540, 975
Hpy188III TCNNGA 4 cut(s) 125, 234, 251, 941
Hpy8I GTNNAC 3 cut(s) 343, 742, 969
Hpy99I CGWCG 4 cut(s) 66, 152, 155, 729
HpyAV CCTTC 2 cut(s) 122, 257
HpyCH4III ACNGT 2 cut(s) 420, 463
HpyCH4IV ACGT 1 cut(s) 150
HpyCH4V TGCA 5 cut(s) 277, 292, 405, 605, 830
HpyF10VI GCNNNNNNNGC 7 cut(s) 211, 274, 644, 653, 656, 721, 839
HpyF3I CTNAG 3 cut(s) 537, 555, 954
HpySE526I ACGT 1 cut(s) 150
Hsp92I GRCGYC 1 cut(s) 150
Hsp92II CATG 3 cut(s) 199, 599, 674
HspAI GCGC 1 cut(s) 193
Kzo9I GATC 4 cut(s) 111, 121, 396, 703
LguI GCTCTTC 1 cut(s) 177
Lsp1109I GCAGC 6 cut(s) 439, 634, 643, 644, 647, 842
LweI GCATC 4 cut(s) 239, 350, 592, 870
MaeI CTAG 2 cut(s) 900, 999
MaeII ACGT 1 cut(s) 150
MalI GATC 4 cut(s) 113, 123, 398, 705
MboI GATC 4 cut(s) 111, 121, 396, 703
MboII GAAGA 5 cut(s) 10, 194, 292, 530, 780
MhlI GDGCHC 1 cut(s) 216
MluCI AATT 3 cut(s) 333, 406, 446
MlyI GAGTC 1 cut(s) 177
MmeI TCCRAC 1 cut(s) 826
MseI TTAA 3 cut(s) 546, 585, 696
MslI CAYNNNNRTG 1 cut(s) 600
MspA1I CMGCKG 2 cut(s) 430, 833
MspCI CTTAAG 1 cut(s) 695
MspI CCGG 1 cut(s) 1003
MspR9I CCNGG 1 cut(s) 749
Mva1269I GAATGC 1 cut(s) 868
MvaI CCWGG 1 cut(s) 749
MvnI CGCG 2 cut(s) 147, 715
MwoI GCNNNNNNNGC 7 cut(s) 211, 274, 644, 653, 656, 721, 839
NdeII GATC 4 cut(s) 111, 121, 396, 703
NheI GCTAGC 1 cut(s) 998
NlaIII CATG 3 cut(s) 199, 599, 674
NlaIV GGNNCC 3 cut(s) 213, 214, 951
NspI RCATGY 1 cut(s) 199
PaeI GCATGC 1 cut(s) 199
PciSI GCTCTTC 1 cut(s) 177
PctI GAATGC 1 cut(s) 868
PfeI GAWTC 3 cut(s) 220, 283, 496
PkrI GCNGC 6 cut(s) 429, 634, 637, 649, 658, 832
PleI GAGTC 1 cut(s) 176
PpsI GAGTC 1 cut(s) 176
Psp6I CCWGG 1 cut(s) 747
PspFI CCCAGC 2 cut(s) 512, 833
PspGI CCWGG 1 cut(s) 747
PspN4I GGNNCC 3 cut(s) 213, 214, 951
PspOMI GGGCCC 1 cut(s) 212
PspPI GGNCC 2 cut(s) 212, 213
PstI CTGCAG 2 cut(s) 294, 832
PvuII CAGCTG 1 cut(s) 833
RsaI GTAC 2 cut(s) 628, 904
RsaNI GTAC 2 cut(s) 627, 903
RseI CAYNNNNRTG 1 cut(s) 600
SapI GCTCTTC 1 cut(s) 177
SaqAI TTAA 3 cut(s) 546, 585, 696
SatI GCNGC 6 cut(s) 428, 633, 636, 648, 657, 831
Sau3AI GATC 4 cut(s) 111, 121, 396, 703
Sau96I GGNCC 2 cut(s) 212, 213
ScaI AGTACT 1 cut(s) 904
SchI GAGTC 1 cut(s) 177
ScrFI CCNGG 1 cut(s) 749
SduI GDGCHC 1 cut(s) 216
SfaNI GCATC 4 cut(s) 239, 350, 592, 870
SfcI CTRYAG 2 cut(s) 290, 828
SmiMI CAYNNNNRTG 1 cut(s) 600
SmlI CTYRAG 1 cut(s) 695
SmoI CTYRAG 1 cut(s) 695
SphI GCATGC 1 cut(s) 199
Sse9I AATT 3 cut(s) 333, 406, 446
SsiI CCGC 8 cut(s) 74, 145, 324, 430, 701, 715, 794, 807
SspMI CTAG 2 cut(s) 900, 999
StyD4I CCNGG 1 cut(s) 747
TaaI ACNGT 2 cut(s) 420, 463
TaiI ACGT 1 cut(s) 153
TaqI TCGA 4 cut(s) 114, 235, 422, 727
TasI AATT 3 cut(s) 333, 406, 446
TatI WGTACW 2 cut(s) 626, 902
TfiI GAWTC 3 cut(s) 220, 283, 496
Tru1I TTAA 3 cut(s) 546, 585, 696
Tru9I TTAA 3 cut(s) 546, 585, 696
TscAI CASTG 3 cut(s) 169, 466, 1000
TseI GCWGC 6 cut(s) 427, 632, 635, 647, 656, 830
TspDTI ATGAA 3 cut(s) 564, 791, 1004
TspGWI ACGGA 1 cut(s) 585
TspRI CASTG 3 cut(s) 169, 466, 1000
Vha464I CTTAAG 1 cut(s) 695
XceI RCATGY 1 cut(s) 199
XmiI GTMKAC 2 cut(s) 342, 968
XspI CTAG 2 cut(s) 900, 999
ZraI GACGTC 1 cut(s) 151
ZrmI AGTACT 1 cut(s) 904
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.