Rmu_sc0000749.1_g000053

Transcription initiation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000749.1
OG0001612
Physical Location & Seq
Reverse (-)
234051 .. 235010
960 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 960 bp
ctgtttcctcctgtcaaaaaccctaacaagcgtagagcttctccaccaccaagtaagaagcaaaaggtagctgatgatgagatcgacacagatcaagaaggcattgagcaactctgcgacgtcctttctgtcagtggagtcaatcttgtggaagagcaagggcgcatgctgttgcttccggggcccaagaagcagcaagcatctcgaaaacttgtgtttcaagaaaaccaagaagggcaggttttgcagaagattcccctgcagaaaaagttggcgggaatcatggggaggtgtggtttgagaattggtgttgacaaagatgtggagagattcttgtcaatgtgtgtggaggagagaatgagggggatcatatgcaatttgattagacagtcgaagcagtgggttgattgcgaaaaatcgagacaccacactgtttctatatctgatgtgaagcaagagattatgattctcaacaggaaagctagggaagagttgcaggaaaaggctcaaaagcttaatgaagctgaggttaacactaacggatttgagggtgttcaggacaaacatgatcatggagaagaaaaaatgaggagaagtacagcagcagcaaatgttgctgcccttgctgctcttggaggacatgacatattggcaaaatggcaacttaaggcggatcagctggccagggagaagcgtcgaggtaaagatgtaaaccacaaggctacatcaactgctagaagaacaatgaatggcaatgaagaagcagagaaaaggggcagtgtagaagctttggtgtctgcagctgggagtgctgttcgaaagtttgggaggaatgcccaagttacgatgatgcctcaaacaaaagtggctagtactcgtagtatatctgtcaaggatgtgattgctgtgatgtccaagtctactctgatctatttactaatgaacactgctagctggtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000428 GO:0001650 GO:0003674 GO:0003676 GO:0003677 GO:0003712 GO:0003713 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005667 GO:0005669 GO:0005730 GO:0005911 GO:0006139 GO:0006351 GO:0006352 GO:0006355 GO:0006357 GO:0006366 GO:0006367 GO:0006725 GO:0006807 GO:0008134 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009506 GO:0009889 GO:0009891 GO:0009893 GO:0009966 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0010646 GO:0016070 GO:0016591 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0023051 GO:0030054 GO:0030880 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031974 GO:0031981 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044452 GO:0044464 GO:0044798 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048522 GO:0048583 GO:0050789 GO:0050794 GO:0051059 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0055029 GO:0055044 GO:0060255 GO:0061695 GO:0065007 GO:0070013 GO:0071704 GO:0080090 GO:0090304 GO:0090575 GO:0097159 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1901796 GO:1902494 GO:1902531 GO:1902680 GO:1903506 GO:1903508 GO:1990234 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03022 ko05016 ko05168 map03022 map05016 map05168
Pfam Domains
Protein Families
RST TAF4

Protein Analysis

319

Amino Acids

35.57

Weight (kDa)

9.61

Isoelectric Point (pI)

48.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TAF4 PF05236 39 - 305 1.2e-39 Transcription initiation factor TFIID component TAF4 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 123
Acc36I ACCTGC 1 cut(s) 229
AccI GTMKAC 1 cut(s) 920
AciI CCGC 2 cut(s) 275, 671
AclWI GGATC 2 cut(s) 374, 681
AcoI YGGCCR 1 cut(s) 681
AcyI GRCGYC 1 cut(s) 120
AfaI GTAC 2 cut(s) 598, 874
AflII CTTAAG 1 cut(s) 665
AgsI TTSAA 1 cut(s) 221
AjnI CCWGG 1 cut(s) 683
AluBI AGCT 9 cut(s) 38, 71, 482, 514, 524, 679, 788, 803, 954
AluI AGCT 9 cut(s) 38, 71, 482, 514, 524, 679, 788, 803, 954
Alw26I GTCTC 1 cut(s) 415
AlwI GGATC 2 cut(s) 374, 681
AoxI GGCC 2 cut(s) 182, 681
ApaI GGGCCC 1 cut(s) 186
ApeKI GCWGC 6 cut(s) 193, 602, 605, 617, 626, 800
AspLEI GCGC 1 cut(s) 165
AspS9I GGNCC 2 cut(s) 182, 183
AsuC2I CCSGG 1 cut(s) 180
AsuII TTCGAA 1 cut(s) 817
AsuNHI GCTAGC 1 cut(s) 950
BaeGI GKGCMC 1 cut(s) 186
BalI TGGCCA 1 cut(s) 683
BanII GRGCYC 1 cut(s) 186
BbvCI CCTCAGC 1 cut(s) 525
BbvI GCAGC 6 cut(s) 205, 604, 613, 614, 617, 812
BcgI CGANNNNNNTGC 2 cut(s) 185, 219
BciT130I CCWGG 1 cut(s) 685
BclI TGATCA 1 cut(s) 568
BcnI CCSGG 1 cut(s) 180
BcoDI GTCTC 1 cut(s) 415
BfaI CTAG 4 cut(s) 483, 735, 870, 951
BfmI CTRYAG 2 cut(s) 260, 798
BfrI CTTAAG 1 cut(s) 665
BfuAI ACCTGC 1 cut(s) 229
BisI GCNGC 6 cut(s) 194, 603, 606, 618, 627, 801
BlsI GCNGC 6 cut(s) 195, 604, 607, 619, 628, 802
BmcAI AGTACT 1 cut(s) 874
Bme1390I CCNGG 2 cut(s) 180, 685
BmgT120I GGNCC 2 cut(s) 182, 183
BmiI GGNNCC 2 cut(s) 183, 184
BmrFI CCNGG 2 cut(s) 180, 685
BmsI GCATC 2 cut(s) 209, 840
BmtI GCTAGC 1 cut(s) 954
Bpu10I CCTNAGC 1 cut(s) 525
Bpu14I TTCGAA 1 cut(s) 817
BpuMI CCSGG 1 cut(s) 180
BsaHI GRCGYC 1 cut(s) 120
BsaJI CCNNGG 2 cut(s) 179, 684
BsaXI ACNNNNNCTCC 2 cut(s) 280, 310
Bse3DI GCAATG 1 cut(s) 760
BseBI CCWGG 1 cut(s) 685
BseDI CCNNGG 2 cut(s) 179, 684
BseGI GGATG 1 cut(s) 901
BseMI GCAATG 1 cut(s) 760
BseMII CTCAG 1 cut(s) 516
BseRI GAGGAG 2 cut(s) 365, 604
BseSI GKGCMC 1 cut(s) 186
BseXI GCAGC 6 cut(s) 205, 604, 613, 614, 617, 812
BseYI CCCAGC 1 cut(s) 803
BshFI GGCC 2 cut(s) 184, 683
BsiSI CCGG 1 cut(s) 179
BsmAI GTCTC 1 cut(s) 415
BsmI GAATGC 1 cut(s) 838
BsnI GGCC 2 cut(s) 184, 683
Bsp119I TTCGAA 1 cut(s) 817
Bsp120I GGGCCC 1 cut(s) 182
Bsp1286I GDGCHC 1 cut(s) 186
Bsp143I GATC 6 cut(s) 81, 91, 366, 568, 673, 927
BspACI CCGC 2 cut(s) 275, 671
BspANI GGCC 2 cut(s) 184, 683
BspCNI CTCAG 1 cut(s) 517
BspLI GGNNCC 2 cut(s) 183, 184
BspMAI CTGCAG 2 cut(s) 264, 802
BspMI ACCTGC 1 cut(s) 229
BspOI GCTAGC 1 cut(s) 954
BspPI GGATC 2 cut(s) 374, 681
BspQI GCTCTTC 1 cut(s) 147
BspT104I TTCGAA 1 cut(s) 817
BspTI CTTAAG 1 cut(s) 665
BsrDI GCAATG 1 cut(s) 760
BssECI CCNNGG 2 cut(s) 179, 684
BssMI GATC 6 cut(s) 81, 91, 366, 568, 673, 927
BssNI GRCGYC 1 cut(s) 120
Bst2UI CCWGG 1 cut(s) 685
Bst4CI ACNGT 2 cut(s) 390, 433
Bst6I CTCTTC 2 cut(s) 147, 483
BstACI GRCGYC 1 cut(s) 120
BstAFI CTTAAG 1 cut(s) 665
BstAPI GCANNNNNTGC 2 cut(s) 244, 614
BstBI TTCGAA 1 cut(s) 817
BstC8I GCNNGC 4 cut(s) 167, 198, 681, 952
BstDEI CTNAG 1 cut(s) 525
BstF5I GGATG 1 cut(s) 901
BstHHI GCGC 1 cut(s) 165
BstKTI GATC 6 cut(s) 84, 94, 369, 571, 676, 930
BstMAI GTCTC 1 cut(s) 415
BstMBI GATC 6 cut(s) 81, 91, 366, 568, 673, 927
BstMWI GCNNNNNNNGC 7 cut(s) 181, 190, 244, 614, 623, 626, 809
BstNI CCWGG 1 cut(s) 685
BstNSI RCATGY 1 cut(s) 169
BstSCI CCNGG 2 cut(s) 178, 683
BstSFI CTRYAG 2 cut(s) 260, 798
BstSLI GKGCMC 1 cut(s) 186
BstV1I GCAGC 6 cut(s) 205, 604, 613, 614, 617, 812
BsuRI GGCC 2 cut(s) 184, 683
BtsCI GGATG 1 cut(s) 901
BtsI GCAGTG 3 cut(s) 404, 784, 945
BtsIMutI CAGTG 5 cut(s) 139, 404, 429, 784, 945
BveI ACCTGC 1 cut(s) 229
Cac8I GCNNGC 4 cut(s) 167, 198, 681, 952
CfoI GCGC 1 cut(s) 165
Cfr13I GGNCC 2 cut(s) 182, 183
CseI GACGC 1 cut(s) 683
Csp6I GTAC 2 cut(s) 597, 873
CspCI CAANNNNNGTGG 2 cut(s) 327, 362
CviAII CATG 5 cut(s) 166, 283, 566, 572, 641
CviQI GTAC 2 cut(s) 597, 873
DdeI CTNAG 1 cut(s) 525
DpnI GATC 6 cut(s) 83, 93, 368, 570, 675, 929
DpnII GATC 6 cut(s) 81, 91, 366, 568, 673, 927
EaeI YGGCCR 1 cut(s) 681
Eam1104I CTCTTC 2 cut(s) 147, 483
EarI CTCTTC 2 cut(s) 147, 483
EciI GGCGGA 1 cut(s) 686
Eco24I GRGCYC 1 cut(s) 186
EcoO109I RGGNCCY 1 cut(s) 182
EcoRII CCWGG 1 cut(s) 683
EcoT38I GRGCYC 1 cut(s) 186
FaeI CATG 5 cut(s) 169, 286, 569, 575, 644
FatI CATG 5 cut(s) 165, 282, 565, 571, 640
FauI CCCGC 1 cut(s) 268
FauNDI CATATG 1 cut(s) 371
FbaI TGATCA 1 cut(s) 568
FblI GTMKAC 1 cut(s) 920
Fnu4HI GCNGC 6 cut(s) 194, 603, 606, 618, 627, 801
FokI GGATG 1 cut(s) 908
FriOI GRGCYC 1 cut(s) 186
Fsp4HI GCNGC 6 cut(s) 194, 603, 606, 618, 627, 801
FspBI CTAG 4 cut(s) 483, 735, 870, 951
GlaI GCGC 1 cut(s) 164
GluI GCNGC 6 cut(s) 194, 603, 606, 618, 627, 801
GsaI CCCAGC 1 cut(s) 807
HaeIII GGCC 2 cut(s) 184, 683
HapII CCGG 1 cut(s) 179
HgaI GACGC 1 cut(s) 683
HhaI GCGC 1 cut(s) 165
Hin1I GRCGYC 1 cut(s) 120
Hin1II CATG 5 cut(s) 169, 286, 569, 575, 644
Hin6I GCGC 1 cut(s) 163
HinP1I GCGC 1 cut(s) 163
HincII GTYRAC 2 cut(s) 313, 532
HindII GTYRAC 2 cut(s) 313, 532
HindIII AAGCTT 2 cut(s) 512, 786
HinfI GANTC 5 cut(s) 138, 253, 279, 330, 466
HpaI GTTAAC 1 cut(s) 532
HpaII CCGG 1 cut(s) 179
Hpy166II GTNNAC 4 cut(s) 313, 532, 712, 921
Hpy188I TCNGA 2 cut(s) 445, 927
Hpy188III TCNNGA 5 cut(s) 95, 204, 221, 420, 557
Hpy8I GTNNAC 4 cut(s) 313, 532, 712, 921
Hpy99I CGWCG 2 cut(s) 122, 699
HpyAV CCTTC 2 cut(s) 92, 227
HpyCH4III ACNGT 2 cut(s) 390, 433
HpyCH4IV ACGT 1 cut(s) 120
HpyCH4V TGCA 5 cut(s) 247, 262, 375, 496, 800
HpyF10VI GCNNNNNNNGC 7 cut(s) 181, 190, 244, 614, 623, 626, 809
HpyF3I CTNAG 1 cut(s) 525
HpySE526I ACGT 1 cut(s) 120
Hsp92I GRCGYC 1 cut(s) 120
Hsp92II CATG 5 cut(s) 169, 286, 569, 575, 644
HspAI GCGC 1 cut(s) 163
Ksp22I TGATCA 1 cut(s) 568
KspAI GTTAAC 1 cut(s) 532
Kzo9I GATC 6 cut(s) 81, 91, 366, 568, 673, 927
LguI GCTCTTC 1 cut(s) 147
Lsp1109I GCAGC 6 cut(s) 205, 604, 613, 614, 617, 812
LweI GCATC 2 cut(s) 209, 840
MaeI CTAG 4 cut(s) 483, 735, 870, 951
MaeII ACGT 1 cut(s) 120
MaeIII GTNAC 1 cut(s) 841
MalI GATC 6 cut(s) 83, 93, 368, 570, 675, 929
MboI GATC 6 cut(s) 81, 91, 366, 568, 673, 927
MboII GAAGA 6 cut(s) 164, 262, 500, 590, 750, 770
MhlI GDGCHC 1 cut(s) 186
MlsI TGGCCA 1 cut(s) 683
MluCI AATT 2 cut(s) 303, 376
MluNI TGGCCA 1 cut(s) 683
MlyI GAGTC 1 cut(s) 147
Mox20I TGGCCA 1 cut(s) 683
MscI TGGCCA 1 cut(s) 683
MseI TTAA 3 cut(s) 516, 531, 666
MslI CAYNNNNRTG 1 cut(s) 570
Msp20I TGGCCA 1 cut(s) 683
MspA1I CMGCKG 2 cut(s) 679, 803
MspCI CTTAAG 1 cut(s) 665
MspI CCGG 1 cut(s) 179
MspR9I CCNGG 2 cut(s) 180, 685
Mva1269I GAATGC 1 cut(s) 838
MvaI CCWGG 1 cut(s) 685
MwoI GCNNNNNNNGC 7 cut(s) 181, 190, 244, 614, 623, 626, 809
NciI CCSGG 1 cut(s) 180
NdeI CATATG 1 cut(s) 371
NdeII GATC 6 cut(s) 81, 91, 366, 568, 673, 927
NheI GCTAGC 1 cut(s) 950
NlaIII CATG 5 cut(s) 169, 286, 569, 575, 644
NlaIV GGNNCC 2 cut(s) 183, 184
NspI RCATGY 1 cut(s) 169
NspV TTCGAA 1 cut(s) 817
PaeI GCATGC 1 cut(s) 169
PciSI GCTCTTC 1 cut(s) 147
PctI GAATGC 1 cut(s) 838
PfeI GAWTC 4 cut(s) 253, 279, 330, 466
PkrI GCNGC 6 cut(s) 195, 604, 607, 619, 628, 802
PleI GAGTC 1 cut(s) 146
PpsI GAGTC 1 cut(s) 146
Psp6I CCWGG 1 cut(s) 683
PspFI CCCAGC 1 cut(s) 803
PspGI CCWGG 1 cut(s) 683
PspN4I GGNNCC 2 cut(s) 183, 184
PspOMI GGGCCC 1 cut(s) 182
PspPI GGNCC 2 cut(s) 182, 183
PstI CTGCAG 2 cut(s) 264, 802
PvuII CAGCTG 2 cut(s) 679, 803
RsaI GTAC 2 cut(s) 598, 874
RsaNI GTAC 2 cut(s) 597, 873
RseI CAYNNNNRTG 1 cut(s) 570
SapI GCTCTTC 1 cut(s) 147
SaqAI TTAA 3 cut(s) 516, 531, 666
SatI GCNGC 6 cut(s) 194, 603, 606, 618, 627, 801
Sau3AI GATC 6 cut(s) 81, 91, 366, 568, 673, 927
Sau96I GGNCC 2 cut(s) 182, 183
ScaI AGTACT 1 cut(s) 874
SchI GAGTC 1 cut(s) 147
ScrFI CCNGG 2 cut(s) 180, 685
SduI GDGCHC 1 cut(s) 186
SfaNI GCATC 2 cut(s) 209, 840
SfcI CTRYAG 2 cut(s) 260, 798
SfuI TTCGAA 1 cut(s) 817
SmiMI CAYNNNNRTG 1 cut(s) 570
SmlI CTYRAG 1 cut(s) 665
SmoI CTYRAG 1 cut(s) 665
SphI GCATGC 1 cut(s) 169
Sse9I AATT 2 cut(s) 303, 376
SsiI CCGC 2 cut(s) 275, 671
SspMI CTAG 4 cut(s) 483, 735, 870, 951
StyD4I CCNGG 2 cut(s) 178, 683
TaaI ACNGT 2 cut(s) 390, 433
TaiI ACGT 1 cut(s) 123
TaqI TCGA 6 cut(s) 84, 205, 392, 419, 697, 817
TasI AATT 2 cut(s) 303, 376
TatI WGTACW 2 cut(s) 596, 872
TfiI GAWTC 4 cut(s) 253, 279, 330, 466
Tru1I TTAA 3 cut(s) 516, 531, 666
Tru9I TTAA 3 cut(s) 516, 531, 666
TscAI CASTG 5 cut(s) 139, 404, 436, 784, 952
TseI GCWGC 6 cut(s) 193, 602, 605, 617, 626, 800
TspDTI ATGAA 4 cut(s) 534, 761, 771, 956
TspGWI ACGGA 1 cut(s) 555
TspRI CASTG 5 cut(s) 139, 404, 436, 784, 952
Vha464I CTTAAG 1 cut(s) 665
XceI RCATGY 1 cut(s) 169
XmiI GTMKAC 1 cut(s) 920
XspI CTAG 4 cut(s) 483, 735, 870, 951
ZraI GACGTC 1 cut(s) 121
ZrmI AGTACT 1 cut(s) 874
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.