Rmu_sc0002263.1_g000033

Transcription initiation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002263.1
OG0001612
Physical Location & Seq
Forward (+)
146129 .. 146827
699 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 699 bp
atggggaggtgtggtttgagaattggtgtagacaaagatgtggatagatgcttgtcaatgtgtgtggaggagagaataagggggatcctatgcaatttgattagacagtcgaagcagcgggttgattgcgaaaaatcgaggcaccaccctgtttcgacatctgatgtgaagcaagaaattatgattctcaacaagaaagctagggaagagtttcaggaaaaggctcagaagcttaatgaagctaaggttagcaataacggatttgagggtgttaaggataaacatgacgatggagatgacaaaatgaggagaagtacagcagcagcaaatgttgctgcccttgctgctcttggaggacatgacatattggcaaaatggcaacttaaggcggatcagctggcccgggagaagcgtcgaggtaaagatgtaaaccacaaggctacatcaactgctggaagaacaatgaatggcaatgcagaagcagagaaaaggggcggtgtagaagctttggtgtctgcagctgggagtgctgttggaaagtatgggatgaatgtccaaattatgatgatgcctcaaacaaaggtagctagtactcgtagcatatctgtcaaggacgtgattgctgttctcgaaaaagaacctcagatgtccaagcctactctgatctatttactaatgaacactgcagctggtgaataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000428 GO:0001650 GO:0003674 GO:0003676 GO:0003677 GO:0003712 GO:0003713 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005667 GO:0005669 GO:0005730 GO:0005911 GO:0006139 GO:0006351 GO:0006352 GO:0006355 GO:0006357 GO:0006366 GO:0006367 GO:0006725 GO:0006807 GO:0008134 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009506 GO:0009889 GO:0009891 GO:0009893 GO:0009966 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0010646 GO:0016070 GO:0016591 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0023051 GO:0030054 GO:0030880 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031974 GO:0031981 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044452 GO:0044464 GO:0044798 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048522 GO:0048583 GO:0050789 GO:0050794 GO:0051059 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0055029 GO:0055044 GO:0060255 GO:0061695 GO:0065007 GO:0070013 GO:0071704 GO:0080090 GO:0090304 GO:0090575 GO:0097159 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1901796 GO:1902494 GO:1902531 GO:1902680 GO:1903506 GO:1903508 GO:1990234 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03022 ko05016 ko05168 map03022 map05016 map05168
Pfam Domains
Protein Families
RST TAF4

Protein Analysis

232

Amino Acids

25.45

Weight (kDa)

9.43

Isoelectric Point (pI)

36.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TAF4 PF05236 3 - 223 1e-32 Transcription initiation factor TFIID component TAF4 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 141
AccI GTMKAC 1 cut(s) 30
AciI CCGC 3 cut(s) 118, 389, 495
AclWI GGATC 3 cut(s) 79, 92, 399
AfaI GTAC 2 cut(s) 316, 592
AflII CTTAAG 1 cut(s) 383
AjiI CACGTC 1 cut(s) 616
AluBI AGCT 8 cut(s) 200, 232, 242, 397, 506, 521, 587, 689
AluI AGCT 8 cut(s) 200, 232, 242, 397, 506, 521, 587, 689
AlwI GGATC 3 cut(s) 79, 92, 399
Ama87I CYCGRG 1 cut(s) 402
AoxI GGCC 1 cut(s) 399
ApeKI GCWGC 7 cut(s) 115, 320, 323, 335, 344, 518, 686
Asp700I GAANNNNTTC 1 cut(s) 210
AspS9I GGNCC 1 cut(s) 400
AsuC2I CCSGG 2 cut(s) 403, 404
AvaI CYCGRG 1 cut(s) 402
BamHI GGATCC 1 cut(s) 84
BanI GGYRCC 1 cut(s) 141
BbvI GCAGC 6 cut(s) 127, 322, 331, 332, 335, 530
BccI CCATC 1 cut(s) 284
BcnI CCSGG 2 cut(s) 403, 404
BfaI CTAG 2 cut(s) 201, 588
BfmI CTRYAG 2 cut(s) 516, 684
BfrI CTTAAG 1 cut(s) 383
BisI GCNGC 7 cut(s) 116, 321, 324, 336, 345, 519, 687
BlsI GCNGC 7 cut(s) 117, 322, 325, 337, 346, 520, 688
BmcAI AGTACT 1 cut(s) 592
Bme1390I CCNGG 2 cut(s) 403, 404
BmeT110I CYCGRG 1 cut(s) 402
BmgBI CACGTC 1 cut(s) 616
BmgT120I GGNCC 1 cut(s) 400
BmiI GGNNCC 2 cut(s) 86, 143
BmrFI CCNGG 2 cut(s) 403, 404
BmsI GCATC 2 cut(s) 38, 558
Bpu10I CCTNAGC 1 cut(s) 243
BpuMI CCSGG 2 cut(s) 403, 404
BsaJI CCNNGG 1 cut(s) 402
BsaXI ACNNNNNCTCC 1 cut(s) 28
Bse3DI GCAATG 1 cut(s) 478
BseDI CCNNGG 1 cut(s) 402
BseGI GGATG 1 cut(s) 552
BseMI GCAATG 1 cut(s) 478
BseMII CTCAG 2 cut(s) 239, 656
BseRI GAGGAG 2 cut(s) 83, 322
BseXI GCAGC 6 cut(s) 127, 322, 331, 332, 335, 530
BseYI CCCAGC 1 cut(s) 521
BshFI GGCC 1 cut(s) 401
BshNI GGYRCC 1 cut(s) 141
BsiHKCI CYCGRG 1 cut(s) 402
BsiSI CCGG 1 cut(s) 403
BsnI GGCC 1 cut(s) 401
BsoBI CYCGRG 1 cut(s) 402
Bsp143I GATC 3 cut(s) 84, 391, 663
BspACI CCGC 3 cut(s) 118, 389, 495
BspANI GGCC 1 cut(s) 401
BspCNI CTCAG 2 cut(s) 238, 655
BspLI GGNNCC 2 cut(s) 86, 143
BspMAI CTGCAG 2 cut(s) 520, 688
BspPI GGATC 3 cut(s) 79, 92, 399
BspT107I GGYRCC 1 cut(s) 141
BspTI CTTAAG 1 cut(s) 383
BsrDI GCAATG 1 cut(s) 478
BssECI CCNNGG 1 cut(s) 402
BssMI GATC 3 cut(s) 84, 391, 663
Bst4CI ACNGT 1 cut(s) 108
Bst6I CTCTTC 1 cut(s) 201
BstAFI CTTAAG 1 cut(s) 383
BstAPI GCANNNNNTGC 1 cut(s) 332
BstC8I GCNNGC 1 cut(s) 399
BstDEI CTNAG 3 cut(s) 225, 243, 642
BstF5I GGATG 1 cut(s) 552
BstKTI GATC 3 cut(s) 87, 394, 666
BstMBI GATC 3 cut(s) 84, 391, 663
BstMWI GCNNNNNNNGC 4 cut(s) 332, 341, 344, 527
BstSCI CCNGG 2 cut(s) 401, 402
BstSFI CTRYAG 2 cut(s) 516, 684
BstV1I GCAGC 6 cut(s) 127, 322, 331, 332, 335, 530
BstX2I RGATCY 1 cut(s) 84
BstYI RGATCY 1 cut(s) 84
BsuRI GGCC 1 cut(s) 401
BtrI CACGTC 1 cut(s) 616
BtsCI GGATG 1 cut(s) 552
BtsI GCAGTG 1 cut(s) 681
BtsIMutI CAGTG 1 cut(s) 681
Cac8I GCNNGC 1 cut(s) 399
Cfr13I GGNCC 1 cut(s) 400
Cfr9I CCCGGG 1 cut(s) 402
CseI GACGC 1 cut(s) 401
Csp6I GTAC 2 cut(s) 315, 591
CspCI CAANNNNNGTGG 2 cut(s) 45, 80
CviAII CATG 2 cut(s) 284, 359
CviQI GTAC 2 cut(s) 315, 591
DdeI CTNAG 3 cut(s) 225, 243, 642
DpnI GATC 3 cut(s) 86, 393, 665
DpnII GATC 3 cut(s) 84, 391, 663
Eam1104I CTCTTC 1 cut(s) 201
EarI CTCTTC 1 cut(s) 201
EciI GGCGGA 1 cut(s) 404
Eco88I CYCGRG 1 cut(s) 402
FaeI CATG 2 cut(s) 287, 362
FaiI YATR 8 cut(s) 91, 182, 285, 360, 365, 543, 563, 602
FatI CATG 2 cut(s) 283, 358
FauI CCCGC 1 cut(s) 111
FblI GTMKAC 1 cut(s) 30
Fnu4HI GCNGC 7 cut(s) 116, 321, 324, 336, 345, 519, 687
FokI GGATG 1 cut(s) 559
Fsp4HI GCNGC 7 cut(s) 116, 321, 324, 336, 345, 519, 687
FspBI CTAG 2 cut(s) 201, 588
GluI GCNGC 7 cut(s) 116, 321, 324, 336, 345, 519, 687
GsaI CCCAGC 1 cut(s) 525
HaeIII GGCC 1 cut(s) 401
HapII CCGG 1 cut(s) 403
HgaI GACGC 1 cut(s) 401
Hin1II CATG 2 cut(s) 287, 362
HindIII AAGCTT 2 cut(s) 230, 504
HinfI GANTC 1 cut(s) 184
HpaII CCGG 1 cut(s) 403
Hpy166II GTNNAC 2 cut(s) 31, 430
Hpy188I TCNGA 4 cut(s) 163, 228, 645, 663
Hpy188III TCNNGA 2 cut(s) 215, 629
Hpy8I GTNNAC 2 cut(s) 31, 430
Hpy99I CGWCG 1 cut(s) 417
HpyCH4III ACNGT 1 cut(s) 108
HpyCH4IV ACGT 1 cut(s) 615
HpyCH4V TGCA 4 cut(s) 93, 476, 518, 686
HpyF10VI GCNNNNNNNGC 4 cut(s) 332, 341, 344, 527
HpyF3I CTNAG 3 cut(s) 225, 243, 642
HpySE526I ACGT 1 cut(s) 615
Hsp92II CATG 2 cut(s) 287, 362
Kzo9I GATC 3 cut(s) 84, 391, 663
LpnPI CCDG 7 cut(s) 162, 200, 383, 416, 438, 507, 675
Lsp1109I GCAGC 6 cut(s) 127, 322, 331, 332, 335, 530
LweI GCATC 2 cut(s) 38, 558
MaeI CTAG 2 cut(s) 201, 588
MaeII ACGT 1 cut(s) 615
MalI GATC 3 cut(s) 86, 393, 665
MboI GATC 3 cut(s) 84, 391, 663
MboII GAAGA 2 cut(s) 218, 468
MflI RGATCY 1 cut(s) 84
MluCI AATT 4 cut(s) 21, 94, 177, 558
MmeI TCCRAC 1 cut(s) 514
MnlI CCTC 8 cut(s) 61, 132, 259, 300, 347, 410, 582, 651
MroXI GAANNNNTTC 1 cut(s) 210
MseI TTAA 3 cut(s) 234, 273, 384
MslI CAYNNNNRTG 1 cut(s) 288
MspA1I CMGCKG 4 cut(s) 118, 397, 521, 689
MspCI CTTAAG 1 cut(s) 383
MspI CCGG 1 cut(s) 403
MspR9I CCNGG 2 cut(s) 403, 404
MwoI GCNNNNNNNGC 4 cut(s) 332, 341, 344, 527
NciI CCSGG 2 cut(s) 403, 404
NdeII GATC 3 cut(s) 84, 391, 663
NlaIII CATG 2 cut(s) 287, 362
NlaIV GGNNCC 2 cut(s) 86, 143
PdmI GAANNNNTTC 1 cut(s) 210
PfeI GAWTC 1 cut(s) 184
PkrI GCNGC 7 cut(s) 117, 322, 325, 337, 346, 520, 688
PspFI CCCAGC 1 cut(s) 521
PspN4I GGNNCC 2 cut(s) 86, 143
PspPI GGNCC 1 cut(s) 400
PstI CTGCAG 2 cut(s) 520, 688
PsuI RGATCY 1 cut(s) 84
PvuII CAGCTG 3 cut(s) 397, 521, 689
RsaI GTAC 2 cut(s) 316, 592
RsaNI GTAC 2 cut(s) 315, 591
RseI CAYNNNNRTG 1 cut(s) 288
SaqAI TTAA 3 cut(s) 234, 273, 384
SatI GCNGC 7 cut(s) 116, 321, 324, 336, 345, 519, 687
Sau3AI GATC 3 cut(s) 84, 391, 663
Sau96I GGNCC 1 cut(s) 400
ScaI AGTACT 1 cut(s) 592
ScrFI CCNGG 2 cut(s) 403, 404
SfaNI GCATC 2 cut(s) 38, 558
SfcI CTRYAG 2 cut(s) 516, 684
SmaI CCCGGG 1 cut(s) 404
SmiMI CAYNNNNRTG 1 cut(s) 288
SmlI CTYRAG 1 cut(s) 383
SmoI CTYRAG 1 cut(s) 383
Sse9I AATT 4 cut(s) 21, 94, 177, 558
SsiI CCGC 3 cut(s) 118, 389, 495
SspMI CTAG 2 cut(s) 201, 588
StyD4I CCNGG 2 cut(s) 401, 402
TaaI ACNGT 1 cut(s) 108
TaiI ACGT 1 cut(s) 618
TaqI TCGA 5 cut(s) 110, 137, 155, 415, 630
TasI AATT 4 cut(s) 21, 94, 177, 558
TatI WGTACW 2 cut(s) 314, 590
TfiI GAWTC 1 cut(s) 184
Tru1I TTAA 3 cut(s) 234, 273, 384
Tru9I TTAA 3 cut(s) 234, 273, 384
TscAI CASTG 1 cut(s) 688
TseI GCWGC 7 cut(s) 115, 320, 323, 335, 344, 518, 686
TspDTI ATGAA 4 cut(s) 252, 479, 563, 692
TspGWI ACGGA 1 cut(s) 273
TspMI CCCGGG 1 cut(s) 402
TspRI CASTG 1 cut(s) 688
Vha464I CTTAAG 1 cut(s) 383
XmaI CCCGGG 1 cut(s) 402
XmiI GTMKAC 1 cut(s) 30
XmnI GAANNNNTTC 1 cut(s) 210
XspI CTAG 2 cut(s) 201, 588
ZrmI AGTACT 1 cut(s) 592
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.