Rmu_sc0002750.1_g000008

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002750.1
OG0002385
Physical Location & Seq
Reverse (-)
27918 .. 30104
2187 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 744 bp
atggatacccacagaattaagaataaaaccggaaactctgagccaccgaaatcgttactcacaaaccaaaacccccaattcgaaaccctaaccctccaatccaatcgaccccgcttcaattcggctcgggcaataaagggtgcaggtttggctttccaatcatgctcgtatttctcaaaccactattaccgccttagggtccaaagaccatcttcaaagcaaggctataatacacccacctgccagaactgtggctcaacaaatatcagggtacggcactacttgactttcagaaaatgtaattactatcttgtagctcagctagccactgcagcaagcgagacacggaccaagaatcttgagcgcattgctgatgagctcttggacctctcaaagattgagaggcatgattactccatcctcttcaggctcaaaatgggcctcaacaggtatggccccgcaatctcagggatgagtcccacatcttctgaatctgggcctgcttccacagactctaaggtggcggagaaggtcgcatttgatataaagcttgagaagtttgacgcagcggcaaagatcaagatcattaaggaggttaggactttcacagatttgggactaaaggacgctaaagagttggtggaaaaggctcctgttgtgctgaagaagggtgtgaccaaagaggaggcagggcctattgttgacaagcttaaggagttaggtgctacagtggtattggaatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L12

Protein Analysis

247

Amino Acids

27.62

Weight (kDa)

9.62

Isoelectric Point (pI)

35.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L12 PF00542 180 - 246 1.6e-21 Ribosomal protein L7/L12 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 248
Acc36I ACCTGC 2 cut(s) 134, 248
AciI CCGC 5 cut(s) 112, 190, 459, 524, 569
AcuI CTGAAG 2 cut(s) 409, 683
AfaI GTAC 1 cut(s) 273
AfiI CCNNNNNNNGG 1 cut(s) 196
AflII CTTAAG 1 cut(s) 710
AgsI TTSAA 2 cut(s) 118, 216
AluBI AGCT 5 cut(s) 317, 322, 379, 550, 709
AluI AGCT 5 cut(s) 317, 322, 379, 550, 709
Alw21I GWGCWC 1 cut(s) 381
Alw26I GTCTC 1 cut(s) 335
Ama87I CYCGRG 1 cut(s) 126
AoxI GGCC 4 cut(s) 439, 454, 497, 692
ApeKI GCWGC 2 cut(s) 332, 566
AspLEI GCGC 1 cut(s) 366
AspS9I GGNCC 7 cut(s) 199, 348, 385, 439, 455, 497, 692
AsuII TTCGAA 1 cut(s) 81
AsuNHI GCTAGC 1 cut(s) 322
AvaI CYCGRG 1 cut(s) 126
AvaII GGWCC 3 cut(s) 199, 348, 385
AxyI CCTNAGG 1 cut(s) 194
BanII GRGCYC 1 cut(s) 381
Bbv12I GWGCWC 1 cut(s) 381
BbvI GCAGC 2 cut(s) 344, 578
BccI CCATC 2 cut(s) 217, 425
BceAI ACGGC 1 cut(s) 290
BcoDI GTCTC 1 cut(s) 335
BfaI CTAG 1 cut(s) 323
BfmI CTRYAG 2 cut(s) 330, 726
BfrI CTTAAG 1 cut(s) 710
BfuAI ACCTGC 2 cut(s) 134, 248
BisI GCNGC 3 cut(s) 333, 567, 570
BlpI GCTNAGC 1 cut(s) 318
BlsI GCNGC 3 cut(s) 334, 568, 571
Bme18I GGWCC 3 cut(s) 199, 348, 385
BmeT110I CYCGRG 1 cut(s) 126
BmgT120I GGNCC 7 cut(s) 199, 348, 385, 439, 455, 497, 692
BmiI GGNNCC 3 cut(s) 200, 457, 651
BmtI GCTAGC 1 cut(s) 326
Bpu1102I GCTNAGC 1 cut(s) 318
Bpu14I TTCGAA 1 cut(s) 81
BpuEI CTTGAG 2 cut(s) 380, 572
BsaBI GATNNNNATC 1 cut(s) 581
BsaWI WCCGGW 1 cut(s) 29
Bsc4I CCNNNNNNNGG 1 cut(s) 196
Bse21I CCTNAGG 1 cut(s) 194
Bse3DI GCAATG 1 cut(s) 366
Bse8I GATNNNNATC 1 cut(s) 581
BseGI GGATG 2 cut(s) 417, 477
BseJI GATNNNNATC 1 cut(s) 581
BseLI CCNNNNNNNGG 1 cut(s) 196
BseMI GCAATG 1 cut(s) 366
BseMII CTCAG 3 cut(s) 30, 332, 480
BseRI GAGGAG 1 cut(s) 698
BseXI GCAGC 2 cut(s) 344, 578
BsgI GTGCAG 1 cut(s) 162
BshFI GGCC 4 cut(s) 441, 456, 499, 694
BsiHKAI GWGCWC 1 cut(s) 381
BsiHKCI CYCGRG 1 cut(s) 126
BsiSI CCGG 1 cut(s) 30
BslFI GGGAC 2 cut(s) 462, 630
BslI CCNNNNNNNGG 1 cut(s) 196
BsmAI GTCTC 1 cut(s) 335
BsmFI GGGAC 2 cut(s) 462, 630
BsnI GGCC 4 cut(s) 441, 456, 499, 694
BsoBI CYCGRG 1 cut(s) 126
Bsp119I TTCGAA 1 cut(s) 81
Bsp1286I GDGCHC 1 cut(s) 381
Bsp143I GATC 2 cut(s) 576, 582
Bsp1720I GCTNAGC 1 cut(s) 318
BspACI CCGC 5 cut(s) 112, 190, 459, 524, 569
BspANI GGCC 4 cut(s) 441, 456, 499, 694
BspCNI CTCAG 3 cut(s) 31, 331, 479
BspLI GGNNCC 3 cut(s) 200, 457, 651
BspMAI CTGCAG 1 cut(s) 334
BspMI ACCTGC 2 cut(s) 134, 248
BspOI GCTAGC 1 cut(s) 326
BspT104I TTCGAA 1 cut(s) 81
BspTI CTTAAG 1 cut(s) 710
BsrDI GCAATG 1 cut(s) 366
BssMI GATC 2 cut(s) 576, 582
Bst4CI ACNGT 2 cut(s) 251, 730
Bst6I CTCTTC 1 cut(s) 428
BstAFI CTTAAG 1 cut(s) 710
BstBI TTCGAA 1 cut(s) 81
BstC8I GCNNGC 3 cut(s) 324, 337, 501
BstDEI CTNAG 5 cut(s) 39, 194, 318, 466, 516
BstF5I GGATG 2 cut(s) 417, 477
BstHHI GCGC 1 cut(s) 366
BstKTI GATC 2 cut(s) 579, 585
BstMAI GTCTC 1 cut(s) 335
BstMBI GATC 2 cut(s) 576, 582
BstMWI GCNNNNNNNGC 3 cut(s) 149, 323, 332
BstSFI CTRYAG 2 cut(s) 330, 726
BstV1I GCAGC 2 cut(s) 344, 578
BstXI CCANNNNNNTGG 1 cut(s) 251
Bsu36I CCTNAGG 1 cut(s) 194
BsuRI GGCC 4 cut(s) 441, 456, 499, 694
BtsCI GGATG 2 cut(s) 417, 477
BtsI GCAGTG 1 cut(s) 327
BtsIMutI CAGTG 2 cut(s) 327, 735
BveI ACCTGC 2 cut(s) 134, 248
Cac8I GCNNGC 3 cut(s) 324, 337, 501
CfoI GCGC 1 cut(s) 366
Cfr13I GGNCC 7 cut(s) 199, 348, 385, 439, 455, 497, 692
CseI GACGC 2 cut(s) 572, 635
Csp6I GTAC 1 cut(s) 272
CviAII CATG 2 cut(s) 162, 407
CviQI GTAC 1 cut(s) 272
DdeI CTNAG 5 cut(s) 39, 194, 318, 466, 516
DpnI GATC 2 cut(s) 578, 584
DpnII GATC 2 cut(s) 576, 582
Eam1104I CTCTTC 1 cut(s) 428
EarI CTCTTC 1 cut(s) 428
EciI GGCGGA 1 cut(s) 539
Ecl136II GAGCTC 1 cut(s) 379
Eco24I GRGCYC 1 cut(s) 381
Eco47I GGWCC 3 cut(s) 199, 348, 385
Eco53kI GAGCTC 1 cut(s) 379
Eco57I CTGAAG 2 cut(s) 409, 683
Eco81I CCTNAGG 1 cut(s) 194
Eco88I CYCGRG 1 cut(s) 126
EcoICRI GAGCTC 1 cut(s) 379
EcoO109I RGGNCCY 1 cut(s) 692
EcoT38I GRGCYC 1 cut(s) 381
FaeI CATG 2 cut(s) 165, 410
FaiI YATR 5 cut(s) 163, 228, 408, 453, 545
FalI AAGNNNNNCTT 2 cut(s) 196, 228
FaqI GGGAC 2 cut(s) 462, 630
FatI CATG 2 cut(s) 161, 406
FauI CCCGC 2 cut(s) 119, 466
Fnu4HI GCNGC 3 cut(s) 333, 567, 570
FokI GGATG 2 cut(s) 404, 484
FriOI GRGCYC 1 cut(s) 381
Fsp4HI GCNGC 3 cut(s) 333, 567, 570
FspBI CTAG 1 cut(s) 323
GlaI GCGC 1 cut(s) 365
GluI GCNGC 3 cut(s) 333, 567, 570
HaeIII GGCC 4 cut(s) 441, 456, 499, 694
HapII CCGG 1 cut(s) 30
HgaI GACGC 2 cut(s) 572, 635
HhaI GCGC 1 cut(s) 366
Hin1II CATG 2 cut(s) 165, 410
Hin6I GCGC 1 cut(s) 364
HinP1I GCGC 1 cut(s) 364
HincII GTYRAC 1 cut(s) 703
HindII GTYRAC 1 cut(s) 703
HindIII AAGCTT 2 cut(s) 548, 707
HinfI GANTC 4 cut(s) 355, 475, 491, 512
HpaII CCGG 1 cut(s) 30
Hpy166II GTNNAC 1 cut(s) 703
Hpy188I TCNGA 3 cut(s) 40, 293, 490
Hpy188III TCNNGA 2 cut(s) 359, 580
Hpy8I GTNNAC 1 cut(s) 703
HpyAV CCTTC 2 cut(s) 523, 661
HpyCH4III ACNGT 2 cut(s) 251, 730
HpyCH4V TGCA 2 cut(s) 143, 332
HpyF10VI GCNNNNNNNGC 3 cut(s) 149, 323, 332
HpyF3I CTNAG 5 cut(s) 39, 194, 318, 466, 516
Hsp92II CATG 2 cut(s) 165, 410
HspAI GCGC 1 cut(s) 364
Kzo9I GATC 2 cut(s) 576, 582
LmnI GCTCC 1 cut(s) 655
Lsp1109I GCAGC 2 cut(s) 344, 578
MaeI CTAG 1 cut(s) 323
MaeIII GTNAC 2 cut(s) 54, 673
MalI GATC 2 cut(s) 578, 584
MboI GATC 2 cut(s) 576, 582
MboII GAAGA 4 cut(s) 204, 415, 477, 676
MhlI GDGCHC 1 cut(s) 381
MluCI AATT 4 cut(s) 15, 77, 118, 301
MlyI GAGTC 2 cut(s) 484, 506
MnlI CCTC 8 cut(s) 104, 396, 398, 431, 452, 586, 676, 679
MseI TTAA 3 cut(s) 18, 588, 711
MspA1I CMGCKG 1 cut(s) 569
MspCI CTTAAG 1 cut(s) 710
MspI CCGG 1 cut(s) 30
MwoI GCNNNNNNNGC 3 cut(s) 149, 323, 332
NdeII GATC 2 cut(s) 576, 582
NheI GCTAGC 1 cut(s) 322
NlaIII CATG 2 cut(s) 165, 410
NlaIV GGNNCC 3 cut(s) 200, 457, 651
NmuCI GTSAC 1 cut(s) 673
NspV TTCGAA 1 cut(s) 81
PaqCI CACCTGC 1 cut(s) 248
PfeI GAWTC 2 cut(s) 355, 491
PkrI GCNGC 3 cut(s) 334, 568, 571
PleI GAGTC 2 cut(s) 483, 506
PpsI GAGTC 2 cut(s) 483, 506
Psp124BI GAGCTC 1 cut(s) 381
PspN4I GGNNCC 3 cut(s) 200, 457, 651
PspPI GGNCC 7 cut(s) 199, 348, 385, 439, 455, 497, 692
PstI CTGCAG 1 cut(s) 334
RsaI GTAC 1 cut(s) 273
RsaNI GTAC 1 cut(s) 272
SacI GAGCTC 1 cut(s) 381
SaqAI TTAA 3 cut(s) 18, 588, 711
SatI GCNGC 3 cut(s) 333, 567, 570
Sau3AI GATC 2 cut(s) 576, 582
Sau96I GGNCC 7 cut(s) 199, 348, 385, 439, 455, 497, 692
SchI GAGTC 2 cut(s) 484, 506
SduI GDGCHC 1 cut(s) 381
SfcI CTRYAG 2 cut(s) 330, 726
SfuI TTCGAA 1 cut(s) 81
SinI GGWCC 3 cut(s) 199, 348, 385
SmlI CTYRAG 3 cut(s) 359, 551, 710
SmoI CTYRAG 3 cut(s) 359, 551, 710
Sse9I AATT 4 cut(s) 15, 77, 118, 301
SsiI CCGC 5 cut(s) 112, 190, 459, 524, 569
SspMI CTAG 1 cut(s) 323
SstI GAGCTC 1 cut(s) 381
TaaI ACNGT 2 cut(s) 251, 730
TaqI TCGA 2 cut(s) 81, 106
TasI AATT 4 cut(s) 15, 77, 118, 301
TauI GCSGC 1 cut(s) 572
TfiI GAWTC 2 cut(s) 355, 491
Tru1I TTAA 3 cut(s) 18, 588, 711
Tru9I TTAA 3 cut(s) 18, 588, 711
TscAI CASTG 2 cut(s) 334, 735
TseFI GTSAC 1 cut(s) 673
TseI GCWGC 2 cut(s) 332, 566
Tsp45I GTSAC 1 cut(s) 673
TspGWI ACGGA 1 cut(s) 361
TspRI CASTG 2 cut(s) 334, 735
Vha464I CTTAAG 1 cut(s) 710
VpaK11BI GGWCC 3 cut(s) 199, 348, 385
XspI CTAG 1 cut(s) 323
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.