Rmu_sc0003958.1_g000016

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003958.1
OG0001879
Physical Location & Seq
Reverse (-)
84326 .. 85794
1469 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1011 bp
atgtgttcagcaaaagaaactcctgctaattttgtgtttggcgattctctagttgaagtggggaacaacaactatattgcgactctctccaaggctgattatcctcccaatggaatcgatttcggaaagcctaccgggagatacacaaatggaagaaccatcattgacattataggtcagactcttggttttaaggggttcactcctccttacttagctcccacaacagctggaccagtaattctgaggggtgttaactatgcttcaggtggaagtggaattcttaatgaaacaggaaaaatatttatagataactttgcaaataccaggcaagacattatctcaagaatcggtcttcctgcagctctaaagttacttagaagagctctgttctcagtaacaatcggctcgaatgatttcatcaataactacttggcgccagttatatctgagacggagaggaagtccatacctccggaaacctttgttggcattttgatcgcaagactcggactacaaattacaagactgtacaatttgggtgcaaggaagatggttgtggtaaatgtaggacctattgggtgtataccttatcagagggaagtaaatcaagctgatccagagagctgtgcggaatttccgaatcaactatcacggtcattcaatgccaagctaaggacccttgtcaccgagctaaacaagaaccttcctacggccaattttgtttatgcagatgtttatagtattgtggaagacattattgagaactacatatcatatggtttcgagaatgcaaactctgcatgctgcagagtagcagggcgctacgggggtctaattccatgcggtcctcagccatcaaaagtttgcgtggaccggtcgaagtatgtgttttgggatgcttaccacccttctgatgcttctaatatgatcatagcaaggcgtctcttgtatgggaactccagtgatatttcacccatgaatattgtgcaacttctccaagcttcttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

336

Amino Acids

36.67

Weight (kDa)

8.59

Isoelectric Point (pI)

34.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 11 - 315 8.7e-32 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 436
AccI GTMKAC 1 cut(s) 586
AccIII TCCGGA 1 cut(s) 475
AciI CCGC 2 cut(s) 632, 846
AclWI GGATC 1 cut(s) 611
AcoI YGGCCR 1 cut(s) 714
AcsI RAATTY 2 cut(s) 279, 635
AcuI CTGAAG 1 cut(s) 249
AcyI GRCGYC 2 cut(s) 437, 943
AfaI GTAC 1 cut(s) 533
AfiI CCNNNNNNNGG 3 cut(s) 110, 675, 712
AgeI ACCGGT 1 cut(s) 876
AgsI TTSAA 2 cut(s) 56, 664
AjnI CCWGG 1 cut(s) 326
AjuI GAANNNNNNNTTGG 2 cut(s) 471, 503
AluBI AGCT 9 cut(s) 218, 230, 365, 386, 614, 627, 673, 694, 1004
AluI AGCT 9 cut(s) 218, 230, 365, 386, 614, 627, 673, 694, 1004
Alw21I GWGCWC 1 cut(s) 388
Alw26I GTCTC 2 cut(s) 446, 950
AlwI GGATC 1 cut(s) 611
Aor13HI TCCGGA 1 cut(s) 475
AoxI GGCC 1 cut(s) 714
ApeKI GCWGC 2 cut(s) 362, 807
ApoI RAATTY 2 cut(s) 279, 635
AsiGI ACCGGT 1 cut(s) 876
Asp700I GAANNNNTTC 1 cut(s) 416
AspLEI GCGC 2 cut(s) 439, 825
AspS9I GGNCC 5 cut(s) 233, 572, 678, 848, 874
AsuC2I CCSGG 1 cut(s) 136
AsuHPI GGTGA 2 cut(s) 679, 966
AvaII GGWCC 5 cut(s) 233, 572, 678, 848, 874
BanI GGYRCC 1 cut(s) 436
BanII GRGCYC 1 cut(s) 388
BbsI GAAGAC 2 cut(s) 347, 759
Bbv12I GWGCWC 1 cut(s) 388
BbvCI CCTCAGC 1 cut(s) 852
BbvI GCAGC 2 cut(s) 374, 794
BccI CCATC 3 cut(s) 167, 547, 865
BceAI ACGGC 1 cut(s) 729
BcgI CGANNNNNNTGC 2 cut(s) 481, 515
BciT130I CCWGG 1 cut(s) 328
BclI TGATCA 1 cut(s) 930
BcnI CCSGG 1 cut(s) 136
BcoDI GTCTC 2 cut(s) 446, 950
BfaI CTAG 1 cut(s) 50
BfmI CTRYAG 2 cut(s) 360, 808
BfoI RGCGCY 2 cut(s) 440, 826
BisI GCNGC 2 cut(s) 363, 808
BlsI GCNGC 2 cut(s) 364, 809
Bme1390I CCNGG 2 cut(s) 136, 328
Bme18I GGWCC 5 cut(s) 233, 572, 678, 848, 874
BmgT120I GGNCC 5 cut(s) 233, 572, 678, 848, 874
BmiI GGNNCC 2 cut(s) 438, 680
BmrFI CCNGG 2 cut(s) 136, 328
BmsI GCATC 2 cut(s) 889, 907
BoxI GACNNNNGTC 1 cut(s) 683
BpiI GAAGAC 2 cut(s) 347, 759
BpmI CTGGAG 1 cut(s) 946
Bpu10I CCTNAGC 2 cut(s) 674, 852
BpuEI CTTGAG 1 cut(s) 328
BpuMI CCSGG 1 cut(s) 136
Bsa29I ATCGAT 1 cut(s) 117
BsaHI GRCGYC 2 cut(s) 437, 943
BsaJI CCNNGG 1 cut(s) 90
BsaWI WCCGGW 2 cut(s) 475, 876
BsaXI ACNNNNNCTCC 2 cut(s) 449, 479
Bsc4I CCNNNNNNNGG 3 cut(s) 110, 675, 712
Bse118I RCCGGY 1 cut(s) 876
Bse1I ACTGG 3 cut(s) 236, 440, 963
BseAI TCCGGA 1 cut(s) 475
BseBI CCWGG 1 cut(s) 328
BseCI ATCGAT 1 cut(s) 117
BseDI CCNNGG 1 cut(s) 90
BseGI GGATG 1 cut(s) 904
BseLI CCNNNNNNNGG 3 cut(s) 110, 675, 712
BseMII CTCAG 4 cut(s) 236, 408, 441, 866
BseNI ACTGG 3 cut(s) 236, 440, 963
BseRI GAGGAG 1 cut(s) 195
BseXI GCAGC 2 cut(s) 374, 794
Bsh1285I CGRYCG 1 cut(s) 881
BshFI GGCC 1 cut(s) 716
BshNI GGYRCC 1 cut(s) 436
BshTI ACCGGT 1 cut(s) 876
BshVI ATCGAT 1 cut(s) 117
BsiEI CGRYCG 1 cut(s) 881
BsiHKAI GWGCWC 1 cut(s) 388
BsiSI CCGG 3 cut(s) 135, 476, 877
BslI CCNNNNNNNGG 3 cut(s) 110, 675, 712
BsmAI GTCTC 2 cut(s) 446, 950
BsmBI CGTCTC 2 cut(s) 446, 950
BsmI GAATGC 1 cut(s) 796
BsnI GGCC 1 cut(s) 716
Bsp1286I GDGCHC 1 cut(s) 388
Bsp13I TCCGGA 1 cut(s) 475
Bsp1407I TGTACA 1 cut(s) 531
Bsp143I GATC 3 cut(s) 498, 616, 930
BspACI CCGC 2 cut(s) 632, 846
BspANI GGCC 1 cut(s) 716
BspCNI CTCAG 4 cut(s) 237, 407, 442, 865
BspDI ATCGAT 1 cut(s) 117
BspEI TCCGGA 1 cut(s) 475
BspLI GGNNCC 2 cut(s) 438, 680
BspMAI CTGCAG 2 cut(s) 364, 812
BspPI GGATC 1 cut(s) 611
BspQI GCTCTTC 1 cut(s) 376
BspT107I GGYRCC 1 cut(s) 436
BsrFI RCCGGY 1 cut(s) 876
BsrGI TGTACA 1 cut(s) 531
BsrI ACTGG 3 cut(s) 236, 440, 963
BssAI RCCGGY 1 cut(s) 876
BssECI CCNNGG 1 cut(s) 90
BssMI GATC 3 cut(s) 498, 616, 930
BssNAI GTATAC 1 cut(s) 587
BssNI GRCGYC 2 cut(s) 437, 943
BssT1I CCWWGG 1 cut(s) 90
Bst1107I GTATAC 1 cut(s) 587
Bst2UI CCWGG 1 cut(s) 328
Bst4CI ACNGT 2 cut(s) 531, 657
Bst6I CTCTTC 1 cut(s) 376
BstACI GRCGYC 2 cut(s) 437, 943
BstAPI GCANNNNNTGC 1 cut(s) 800
BstAUI TGTACA 1 cut(s) 531
BstC8I GCNNGC 1 cut(s) 805
BstDEI CTNAG 7 cut(s) 214, 245, 377, 394, 450, 674, 852
BstF5I GGATG 1 cut(s) 904
BstH2I RGCGCY 2 cut(s) 440, 826
BstHHI GCGC 2 cut(s) 439, 825
BstKTI GATC 3 cut(s) 501, 619, 933
BstMAI GTCTC 2 cut(s) 446, 950
BstMBI GATC 3 cut(s) 498, 616, 930
BstMCI CGRYCG 1 cut(s) 881
BstMWI GCNNNNNNNGC 1 cut(s) 800
BstNI CCWGG 1 cut(s) 328
BstNSI RCATGY 1 cut(s) 807
BstPAI GACNNNNGTC 1 cut(s) 683
BstSCI CCNGG 2 cut(s) 134, 326
BstSFI CTRYAG 2 cut(s) 360, 808
BstV1I GCAGC 2 cut(s) 374, 794
BstV2I GAAGAC 2 cut(s) 347, 759
BstZ17I GTATAC 1 cut(s) 587
Bsu15I ATCGAT 1 cut(s) 117
BsuRI GGCC 1 cut(s) 716
BsuTUI ATCGAT 1 cut(s) 117
BtsCI GGATG 1 cut(s) 904
BtsIMutI CAGTG 1 cut(s) 970
Cac8I GCNNGC 1 cut(s) 805
CfoI GCGC 2 cut(s) 439, 825
Cfr10I RCCGGY 1 cut(s) 876
Cfr13I GGNCC 5 cut(s) 233, 572, 678, 848, 874
ClaI ATCGAT 1 cut(s) 117
CseI GACGC 1 cut(s) 932
Csp6I GTAC 1 cut(s) 532
CspAI ACCGGT 1 cut(s) 876
CviAII CATG 3 cut(s) 804, 843, 979
CviQI GTAC 1 cut(s) 532
DdeI CTNAG 7 cut(s) 214, 245, 377, 394, 450, 674, 852
DinI GGCGCC 1 cut(s) 438
DpnI GATC 3 cut(s) 500, 618, 932
DpnII GATC 3 cut(s) 498, 616, 930
EaeI YGGCCR 1 cut(s) 714
Eam1104I CTCTTC 1 cut(s) 376
EarI CTCTTC 1 cut(s) 376
Ecl136II GAGCTC 1 cut(s) 386
Eco130I CCWWGG 1 cut(s) 90
Eco24I GRGCYC 1 cut(s) 388
Eco47I GGWCC 5 cut(s) 233, 572, 678, 848, 874
Eco53kI GAGCTC 1 cut(s) 386
Eco57I CTGAAG 1 cut(s) 249
EcoICRI GAGCTC 1 cut(s) 386
EcoO109I RGGNCCY 2 cut(s) 572, 678
EcoRI GAATTC 1 cut(s) 279
EcoRII CCWGG 1 cut(s) 326
EcoT14I CCWWGG 1 cut(s) 90
EcoT38I GRGCYC 1 cut(s) 388
EgeI GGCGCC 1 cut(s) 438
EheI GGCGCC 1 cut(s) 438
ErhI CCWWGG 1 cut(s) 90
Esp3I CGTCTC 2 cut(s) 446, 950
FaeI CATG 3 cut(s) 807, 846, 982
FatI CATG 3 cut(s) 803, 842, 978
FauNDI CATATG 1 cut(s) 778
FbaI TGATCA 1 cut(s) 930
FblI GTMKAC 1 cut(s) 586
Fnu4HI GCNGC 2 cut(s) 363, 808
FokI GGATG 1 cut(s) 911
FriOI GRGCYC 1 cut(s) 388
Fsp4HI GCNGC 2 cut(s) 363, 808
FspBI CTAG 1 cut(s) 50
GlaI GCGC 2 cut(s) 438, 824
GluI GCNGC 2 cut(s) 363, 808
GsuI CTGGAG 1 cut(s) 946
HaeII RGCGCY 2 cut(s) 440, 826
HaeIII GGCC 1 cut(s) 716
HapII CCGG 3 cut(s) 135, 476, 877
HgaI GACGC 1 cut(s) 932
HhaI GCGC 2 cut(s) 439, 825
Hin1I GRCGYC 2 cut(s) 437, 943
Hin1II CATG 3 cut(s) 807, 846, 982
Hin6I GCGC 2 cut(s) 437, 823
HinP1I GCGC 2 cut(s) 437, 823
HincII GTYRAC 1 cut(s) 256
HindII GTYRAC 1 cut(s) 256
HindIII AAGCTT 1 cut(s) 1002
HinfI GANTC 7 cut(s) 44, 82, 114, 181, 348, 507, 643
HpaI GTTAAC 1 cut(s) 256
HpaII CCGG 3 cut(s) 135, 476, 877
HphI GGTGA 2 cut(s) 679, 966
Hpy166II GTNNAC 4 cut(s) 201, 256, 587, 874
Hpy188I TCNGA 8 cut(s) 125, 180, 246, 451, 512, 597, 642, 916
Hpy188III TCNNGA 5 cut(s) 345, 476, 620, 787, 1008
Hpy8I GTNNAC 4 cut(s) 201, 256, 587, 874
HpyAV CCTTC 2 cut(s) 716, 921
HpyCH4III ACNGT 2 cut(s) 531, 657
HpyCH4V TGCA 8 cut(s) 320, 362, 545, 731, 794, 803, 810, 991
HpyF10VI GCNNNNNNNGC 1 cut(s) 800
HpyF3I CTNAG 7 cut(s) 214, 245, 377, 394, 450, 674, 852
Hsp92I GRCGYC 2 cut(s) 437, 943
Hsp92II CATG 3 cut(s) 807, 846, 982
HspAI GCGC 2 cut(s) 437, 823
KasI GGCGCC 1 cut(s) 436
Kpn2I TCCGGA 1 cut(s) 475
Ksp22I TGATCA 1 cut(s) 930
KspAI GTTAAC 1 cut(s) 256
Kzo9I GATC 3 cut(s) 498, 616, 930
LguI GCTCTTC 1 cut(s) 376
LmnI GCTCC 1 cut(s) 223
Lsp1109I GCAGC 2 cut(s) 374, 794
LweI GCATC 2 cut(s) 889, 907
MaeI CTAG 1 cut(s) 50
MaeIII GTNAC 3 cut(s) 372, 397, 685
MalI GATC 3 cut(s) 500, 618, 932
MboI GATC 3 cut(s) 498, 616, 930
MboII GAAGA 5 cut(s) 165, 347, 393, 562, 764
MhlI GDGCHC 1 cut(s) 388
MluCI AATT 8 cut(s) 28, 240, 279, 519, 535, 635, 718, 837
Mly113I GGCGCC 1 cut(s) 437
MlyI GAGTC 3 cut(s) 76, 175, 501
MnlI CCTC 7 cut(s) 114, 216, 240, 453, 483, 591, 861
MroI TCCGGA 1 cut(s) 475
MroXI GAANNNNTTC 1 cut(s) 416
MseI TTAA 3 cut(s) 192, 255, 285
MspA1I CMGCKG 1 cut(s) 230
MspI CCGG 3 cut(s) 135, 476, 877
MspR9I CCNGG 2 cut(s) 136, 328
Mva1269I GAATGC 1 cut(s) 796
MvaI CCWGG 1 cut(s) 328
MwoI GCNNNNNNNGC 1 cut(s) 800
NarI GGCGCC 1 cut(s) 437
NciI CCSGG 1 cut(s) 136
NdeI CATATG 1 cut(s) 778
NdeII GATC 3 cut(s) 498, 616, 930
NlaIII CATG 3 cut(s) 807, 846, 982
NlaIV GGNNCC 2 cut(s) 438, 680
NmuCI GTSAC 1 cut(s) 685
NspI RCATGY 1 cut(s) 807
PaeI GCATGC 1 cut(s) 807
PciSI GCTCTTC 1 cut(s) 376
PctI GAATGC 1 cut(s) 796
PdmI GAANNNNTTC 1 cut(s) 416
PfeI GAWTC 4 cut(s) 44, 114, 348, 643
PinAI ACCGGT 1 cut(s) 876
PkrI GCNGC 2 cut(s) 364, 809
PleI GAGTC 3 cut(s) 76, 175, 501
PluTI GGCGCC 1 cut(s) 440
PpsI GAGTC 3 cut(s) 76, 175, 501
PpuMI RGGWCCY 2 cut(s) 572, 678
PshAI GACNNNNGTC 1 cut(s) 683
Psp124BI GAGCTC 1 cut(s) 388
Psp5II RGGWCCY 2 cut(s) 572, 678
Psp6I CCWGG 1 cut(s) 326
PspGI CCWGG 1 cut(s) 326
PspN4I GGNNCC 2 cut(s) 438, 680
PspPI GGNCC 5 cut(s) 233, 572, 678, 848, 874
PspPPI RGGWCCY 2 cut(s) 572, 678
PstI CTGCAG 2 cut(s) 364, 812
PvuII CAGCTG 1 cut(s) 230
RsaI GTAC 1 cut(s) 533
RsaNI GTAC 1 cut(s) 532
SacI GAGCTC 1 cut(s) 388
SapI GCTCTTC 1 cut(s) 376
SaqAI TTAA 3 cut(s) 192, 255, 285
SatI GCNGC 2 cut(s) 363, 808
Sau3AI GATC 3 cut(s) 498, 616, 930
Sau96I GGNCC 5 cut(s) 233, 572, 678, 848, 874
SchI GAGTC 3 cut(s) 76, 175, 501
ScrFI CCNGG 2 cut(s) 136, 328
SduI GDGCHC 1 cut(s) 388
SfaNI GCATC 2 cut(s) 889, 907
SfcI CTRYAG 2 cut(s) 360, 808
SfoI GGCGCC 1 cut(s) 438
SinI GGWCC 5 cut(s) 233, 572, 678, 848, 874
SmlI CTYRAG 1 cut(s) 343
SmoI CTYRAG 1 cut(s) 343
SphI GCATGC 1 cut(s) 807
Sse9I AATT 8 cut(s) 28, 240, 279, 519, 535, 635, 718, 837
SsiI CCGC 2 cut(s) 632, 846
SspDI GGCGCC 1 cut(s) 436
SspI AATATT 2 cut(s) 303, 985
SspMI CTAG 1 cut(s) 50
SstI GAGCTC 1 cut(s) 388
StyD4I CCNGG 2 cut(s) 134, 326
StyI CCWWGG 1 cut(s) 90
TaaI ACNGT 2 cut(s) 531, 657
TaqI TCGA 4 cut(s) 117, 410, 786, 881
TaqII GACCGA 1 cut(s) 341
TasI AATT 8 cut(s) 28, 240, 279, 519, 535, 635, 718, 837
TatI WGTACW 1 cut(s) 531
TfiI GAWTC 4 cut(s) 44, 114, 348, 643
Tru1I TTAA 3 cut(s) 192, 255, 285
Tru9I TTAA 3 cut(s) 192, 255, 285
TscAI CASTG 1 cut(s) 970
TseFI GTSAC 1 cut(s) 685
TseI GCWGC 2 cut(s) 362, 807
Tsp45I GTSAC 1 cut(s) 685
TspDTI ATGAA 3 cut(s) 303, 409, 995
TspGWI ACGGA 1 cut(s) 470
TspRI CASTG 1 cut(s) 970
VpaK11BI GGWCC 5 cut(s) 233, 572, 678, 848, 874
XapI RAATTY 2 cut(s) 279, 635
XceI RCATGY 1 cut(s) 807
XmiI GTMKAC 1 cut(s) 586
XmnI GAANNNNTTC 1 cut(s) 416
XspI CTAG 1 cut(s) 50
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.