Rmu_sc0006966.1_g000004

Pre-mRNA-splicing factor ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006966.1
OG0002001
Physical Location & Seq
Forward (+)
20039 .. 27297
7259 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3996 bp
atggagccaatgagttcatcaaacccagattctaaaagcgttggtgcggctgaagtggagggttttcgggattctgtcaatgaaatttcagcaaaagttaatacgcttgagaaaagagtgaatgaggtcgagcactattacttgaggaaaggcagtctgcaaccaaccacttcgagggagaaagacagggataaacactttaacaccattaagaagcatcaacaagatgcagcacgtagggaagccgctgctgcaaagagaatgcgagatctgttgaataagttttccgcaatctttaaacagatcactcagcacaagtggtcatggccctttatgtcgcccgttgatgttgaaagtctgggattgcatgactattatcaagttattgagaagcccatggaccttggtacagtacaaagtaaaatggaaaccggtgggtataagagtgttagagagatatatgctgatgtgaggttggtattcaagaatgcaatcaaatacaatgatgacaaagatgatgtccacgtgatggcccaaacactgttggaaaaattagaagaaaagtggttacaactcttgcctaaagttgttgaagaggagaaaagacaagcagatgaggaagcacaggcacaggtagacatgaaacacgctgaggaggttgcttatgccagcatggccaaggatctaagcagcgagctttttgagattgataagtatttgaaagatctcaaaaaagtggtggttcagaagtgcagaaaaatgtcttttgatgataaaagacgacttgggacagctcttacacagttatctcctgaagatctcagcaaggctttggatatagttgctcaggataatcctgagttccaagcaactgcgcaagaggtggatcttgacattgatactcagagcgactacacattatggaggttaaaggtttttgtcaaggatgcattaaaagttaaggcccaagctgctgcacaaattcctgaagccctgggtggcaacaaagttgcaacctccaaaagaaatagggagccctctgatgctgttgccaagcctgccatacgggcttctggacagtcagtcaagtcttcgggctcgggacgtggtgggagttctcatcagcttgatagtacgctaggagaagaggagggtgattcggttgaagggagatatgagattggtgagagcatgcgtttagagatggaatatgattgtgaccgtgcgtggtatgatagagaagaaggaagcacagcggttgatacgagtgatagttcgtctttgttttatggggatgatgctttttgtcgaaagaaggaaacggagttggcgaagagactggctcggagagatggaactaagatgagccttgctcagagcaaaaggttgtctcagcgcagtgcagataatgctcagtgggaggatcgtcagcttctgcgatctggtgctgttagaggtactgaggtgcagagtgagattgatgatgaagatgagaacaaagttatacttcttgttcatgatacaaggcctcctttcctggatgggagagttgtttttaccaagcaagcggagccggttatgccagtaaaggatcccacgtctgatatggccataatttcacgcaaagggtctgctctagttagggaaatccatcaaaagcaaagttcgaataaatcacgtcaacgtttttgggagctttcaggttccaaacttggtgatattcttggtgttgagaaaacagcagagcatattgatgcagatactgctgtagtaggtaaagacggtgaagttgatttcaaagaagatgcaaagtttgctcagcatatgaagaatggggaggctgtgagtgaattcacaaagtcaaaaaccatttccgaacaacgccagtatctgcctatatattctgtgcgggatgagttactgcagaaaattcgagaaaatcaagttattatagttgttggagaaactggttctggaaagacaactcaactgacacaatatctgcatgaagatggttacactgtgaacggtatagtcggttgcacccagccaagacgtgtggcagccatgagtgttgctaaaagagttagtgaagagatggagactgagcttggtgagaaagtgggctatgctattcgttttgaggatgtgacggggccaaaaactgtaataaagtacatgactgatggagtacttatgcgtgaaacacttagagattctgaccttgacaagtaccgtgtagttgtaatggatgaagcccatgagaggtcactatatacagatgtgctgtttgggattttgaaaaaagtcgtcgcccagcgtcgtgattttaagctcattgtgacatctgctactcttaatgctcagaaatttgcagatttctttggaggtgcacctatttttcccattcctggaagaacatttcctgtacataagaagtacttggataccccatgtgaagattatgttgaagctgcagtgacacaggccatgaaaattcacattggcagccctccgggtgatatcctcatcttcatgaccggccaagaggagattgaagcagcctgttattctcttgcagagcgaatggaacaacttatctcctcctcaaagaaagggatgtcaaaactcttgatactccctatgtattcacagctgccagctgacttgcaagcaaagatattccaaaggtctaaagatggggctcgtaaatgcattatagcaacaaatattgctgagacatctttgaccgtagatggtatattttatgtcattgacactggctatggtaaaatgaaagtttacaaccctaggatgggtatggatgctcttcaagtatttcctagtagtcgtgctgctgctgaccaacgtgctgggcgtgctggtagaactgggcctggaacatgttatcggctgtacacagagagtgcctacctaaatgaaatgctggccagtcctgtaccagagatccagaggacgaaccttggctacgtggtcttgttgctcaagtctctgaaggttgataacttgcttgattttgatttcatggacccacctcccctggaaaatattctcaattctatgtaccagctgtgggtcttgggagctctcgacaatgtgggaggtttgactgccttaggctggaaaatggtggagttcccattggaccctccacttgctaagatgttgttgatgggtgaagagctaggatgcgtagatgaaattttgacaattgtatccatgctttcagtgccatcagtattctttaggcccaaagatagggcagaggagagcgatgctgctcgggaaaaattttcagtcccagagtccgatcacctgacattctacaatgtttatcaacagtggaaacaacatcagtatcggggggactggtgcagtgaccattttttgcatgttaaggggttacaaaaggctagagacgttaggtcccagctgctagagattctgaagacgttgaaaattccactgacatcttgttggcctgatactgacgttgtgagaaaagctatttgctctgcatactttcaaaatgctgcaaagtttaagggtgtgggggagtatgttaattgccggactgggatgccatgccatttacacccgagcagtgctctttatggtatgggatgcacccctgagtatgtggtttatcatgaacagattctgacaacaaaggagtacatgcagtgtgtaacagcagtggagccgcagtggttggctgattggggacccatgttcttttctgtaaaggagtcggatacatcagtgttgcagaataagaagagacagcaggaagaaaaaacagctatggaggaggaaatggacaatctgagaaaggctcaagcacgggcagaggcagaaagcaagcagagggagagggaaaagaggtataaacagcagcagcagcagcaagtctcattggcgggtttgcaccagggctcttctacatatctgaggcccaagaaacttggcttgtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000375 GO:0000377 GO:0000398 GO:0002376 GO:0003002 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005681 GO:0005737 GO:0006139 GO:0006396 GO:0006397 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006950 GO:0006952 GO:0006955 GO:0007275 GO:0007389 GO:0008026 GO:0008104 GO:0008150 GO:0008152 GO:0008186 GO:0008380 GO:0009605 GO:0009607 GO:0009620 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009814 GO:0009817 GO:0009888 GO:0009892 GO:0009893 GO:0009987 GO:0010015 GO:0010033 GO:0010051 GO:0010053 GO:0010054 GO:0010154 GO:0010467 GO:0010468 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010629 GO:0014070 GO:0015031 GO:0015833 GO:0015931 GO:0016049 GO:0016070 GO:0016071 GO:0016246 GO:0016441 GO:0016458 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0021700 GO:0022414 GO:0022622 GO:0030154 GO:0031047 GO:0031050 GO:0031053 GO:0031123 GO:0031124 GO:0031323 GO:0031325 GO:0031503 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0033036 GO:0033120 GO:0034470 GO:0034613 GO:0034641 GO:0034660 GO:0035194 GO:0035195 GO:0035196 GO:0040007 GO:0040029 GO:0042221 GO:0042623 GO:0042886 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043331 GO:0043484 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045087 GO:0045184 GO:0045935 GO:0046483 GO:0046907 GO:0048316 GO:0048364 GO:0048468 GO:0048469 GO:0048518 GO:0048519 GO:0048522 GO:0048588 GO:0048589 GO:0048608 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048856 GO:0048869 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051028 GO:0051168 GO:0051169 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051236 GO:0051252 GO:0051254 GO:0051641 GO:0051649 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0060560 GO:0061458 GO:0065007 GO:0070013 GO:0070035 GO:0070727 GO:0070887 GO:0070918 GO:0071013 GO:0071166 GO:0071310 GO:0071359 GO:0071407 GO:0071426 GO:0071427 GO:0071695 GO:0071702 GO:0071704 GO:0071705 GO:0080090 GO:0080147 GO:0090304 GO:0090558 GO:0090627 GO:0097159 GO:0098542 GO:0099402 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1902494 GO:1905392 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
DEAD DUF2075 HA2 Helicase_C OB_NTP_bind

Protein Analysis

1331

Amino Acids

150.25

Weight (kDa)

6.02

Isoelectric Point (pI)

46.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bromodomain PF00439 96 - 179 9.9e-19 Bromodomain
BET PF17035 253 - 316 1.4e-17 Bromodomain extra-terminal - transcription regulation
DEAD PF00270 638 - 790 1.1e-08 DEAD/DEAH box helicase
AAA_22 PF13401 646 - 779 5.1e-06 AAA domain
Helicase_C PF00271 836 - 965 2.2e-10 Helicase conserved C-terminal domain
WHD_HA2 PF04408 1030 - 1055 8.2e-07 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 1056 - 1115 2.6e-15 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 1173 - 1249 7e-18 Oligonucleotide/oligosaccharide-binding (OB)-fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 876
AccB7I CCANNNNNTGG 1 cut(s) 529
AccI GTMKAC 1 cut(s) 636
AciI CCGC 8 cut(s) 47, 246, 288, 1256, 1568, 1909, 3725, 3941
AclI AACGTT 1 cut(s) 1684
AclWI GGATC 6 cut(s) 690, 894, 1431, 1586, 1599, 2962
AcoI YGGCCR 4 cut(s) 675, 1608, 2542, 2949
AcsI RAATTY 9 cut(s) 84, 981, 1850, 1929, 2369, 2496, 3222, 3311, 3480
AcuI CTGAAG 5 cut(s) 72, 834, 1008, 3035, 3488
AcvI CACGTG 1 cut(s) 526
AfiI CCNNNNNNNGG 9 cut(s) 174, 529, 2265, 2411, 2815, 2816, 3094, 3141, 3279
AflIII ACRYGT 2 cut(s) 2056, 2903
AgeI ACCGGT 1 cut(s) 431
AhdI GACNNNNNGTC 2 cut(s) 1082, 3445
AjiI CACGTC 4 cut(s) 1106, 1599, 1679, 2057
AjnI CCWGG 6 cut(s) 993, 1536, 2410, 2896, 3060, 3951
Alw21I GWGCWC 4 cut(s) 135, 2395, 3109, 3631
Alw26I GTCTC 8 cut(s) 1330, 1395, 2096, 2732, 3015, 3432, 3796, 3937
Alw44I GTGCAC 1 cut(s) 2391
AlwI GGATC 6 cut(s) 690, 894, 1431, 1586, 1599, 2962
AlwNI CAGNNNCTG 4 cut(s) 1435, 1763, 1891, 3682
Ama87I CYCGRG 3 cut(s) 1099, 3303, 3619
ApaLI GTGCAC 1 cut(s) 2391
ApoI RAATTY 9 cut(s) 84, 981, 1850, 1929, 2369, 2496, 3222, 3311, 3480
ArsI GACNNNNNNTTYG 2 cut(s) 504, 536
AsiGI ACCGGT 1 cut(s) 431
Asp700I GAANNNNTTC 2 cut(s) 3069, 3678
AspA2I CCTAGG 1 cut(s) 2810
AspLEI GCGC 2 cut(s) 877, 1398
AsuC2I CCSGG 1 cut(s) 2517
AsuHPI GGTGA 8 cut(s) 1166, 1196, 1727, 1796, 2126, 2531, 3209, 3326
AsuII TTCGAA 1 cut(s) 1667
AvaI CYCGRG 3 cut(s) 1099, 3303, 3619
AvaII GGWCC 5 cut(s) 400, 3049, 3166, 3447, 3746
AvrII CCTAGG 1 cut(s) 2810
AxyI CCTNAGG 1 cut(s) 3136
BaeGI GKGCMC 1 cut(s) 2395
BalI TGGCCA 3 cut(s) 677, 1610, 2951
BamHI GGATCC 1 cut(s) 1591
BanII GRGCYC 5 cut(s) 1038, 1100, 2707, 3109, 3959
BbrPI CACGTG 1 cut(s) 526
BbsI GAAGAC 2 cut(s) 1083, 3476
Bbv12I GWGCWC 4 cut(s) 135, 2395, 3109, 3631
BbvCI CCTCAGC 1 cut(s) 651
BcgI CGANNNNNNTGC 2 cut(s) 1913, 1947
BciT130I CCWGG 6 cut(s) 995, 1538, 2412, 2898, 3062, 3953
BciVI GTATCC 3 cut(s) 2440, 3247, 3769
BcnI CCSGG 1 cut(s) 2517
BcoDI GTCTC 8 cut(s) 1330, 1395, 2096, 2732, 3015, 3432, 3796, 3937
BfaI CTAG 7 cut(s) 1139, 1637, 2811, 2844, 3206, 3435, 3458
BfmI CTRYAG 3 cut(s) 1767, 1922, 2475
BfuI GTATCC 3 cut(s) 2440, 3247, 3769
BglI GCCNNNNNGGC 2 cut(s) 674, 1067
BglII AGATCT 3 cut(s) 268, 724, 817
BlnI CCTAGG 1 cut(s) 2810
BlpI GCTNAGC 1 cut(s) 1818
BmcAI AGTACT 2 cut(s) 2193, 2441
Bme1390I CCNGG 7 cut(s) 995, 1538, 2412, 2517, 2898, 3062, 3953
Bme18I GGWCC 5 cut(s) 400, 3049, 3166, 3447, 3746
BmeRI GACNNNNNGTC 2 cut(s) 1082, 3445
BmeT110I CYCGRG 3 cut(s) 1099, 3303, 3619
BmgBI CACGTC 4 cut(s) 1106, 1599, 1679, 2057
BmrFI CCNGG 7 cut(s) 995, 1538, 2412, 2517, 2898, 3062, 3953
BmrI ACTGGG 2 cut(s) 2901, 3606
BmuI ACTGGG 2 cut(s) 2901, 3606
BpiI GAAGAC 2 cut(s) 1083, 3476
BplI GAGNNNNNCTC 8 cut(s) 1327, 1359, 1357, 1389, 3613, 3645, 3841, 3873
Bpu10I CCTNAGC 2 cut(s) 651, 846
Bpu1102I GCTNAGC 1 cut(s) 1818
Bpu14I TTCGAA 1 cut(s) 1667
BpuEI CTTGAG 4 cut(s) 128, 163, 2990, 3843
BpuMI CCSGG 1 cut(s) 2517
BsaAI YACGTR 3 cut(s) 236, 526, 2992
BsaJI CCNNGG 9 cut(s) 396, 403, 678, 993, 994, 2810, 2983, 3060, 3952
BsaWI WCCGGW 1 cut(s) 431
BsaXI ACNNNNNCTCC 4 cut(s) 1513, 1543, 3713, 3743
Bsc4I CCNNNNNNNGG 9 cut(s) 174, 529, 2265, 2411, 2815, 2816, 3094, 3141, 3279
Bse118I RCCGGY 3 cut(s) 431, 1573, 2540
Bse1I ACTGG 9 cut(s) 1344, 1583, 1885, 1972, 2785, 2896, 2952, 3395, 3601
Bse21I CCTNAGG 1 cut(s) 3136
BseBI CCWGG 6 cut(s) 995, 1538, 2412, 2898, 3062, 3953
BseDI CCNNGG 9 cut(s) 396, 403, 678, 993, 994, 2810, 2983, 3060, 3952
BseLI CCNNNNNNNGG 9 cut(s) 174, 529, 2265, 2411, 2815, 2816, 3094, 3141, 3279
BseNI ACTGG 9 cut(s) 1344, 1583, 1885, 1972, 2785, 2896, 2952, 3395, 3601
BseRI GAGGAG 8 cut(s) 611, 668, 1163, 2564, 2593, 2596, 3302, 3845
BseSI GKGCMC 1 cut(s) 2395
BseYI CCCAGC 4 cut(s) 2046, 2316, 2873, 3450
BsgI GTGCAG 5 cut(s) 772, 960, 1422, 1487, 3415
BshTI ACCGGT 1 cut(s) 431
BsiHKAI GWGCWC 4 cut(s) 135, 2395, 3109, 3631
BsiHKCI CYCGRG 3 cut(s) 1099, 3303, 3619
BsiSI CCGG 5 cut(s) 432, 1574, 2516, 2541, 3592
BslFI GGGAC 6 cut(s) 802, 1116, 3305, 3401, 3433, 3759
BslI CCNNNNNNNGG 9 cut(s) 174, 529, 2265, 2411, 2815, 2816, 3094, 3141, 3279
BsmAI GTCTC 8 cut(s) 1330, 1395, 2096, 2732, 3015, 3432, 3796, 3937
BsmBI CGTCTC 1 cut(s) 3432
BsmFI GGGAC 6 cut(s) 802, 1116, 3305, 3401, 3433, 3759
BsmI GAATGC 2 cut(s) 267, 493
BsoBI CYCGRG 3 cut(s) 1099, 3303, 3619
Bsp119I TTCGAA 1 cut(s) 1667
Bsp1286I GDGCHC 8 cut(s) 135, 1038, 1100, 2395, 2707, 3109, 3631, 3959
Bsp1407I TGTACA 2 cut(s) 2428, 2916
Bsp1720I GCTNAGC 1 cut(s) 1818
Bsp19I CCATGG 1 cut(s) 396
BspACI CCGC 8 cut(s) 47, 246, 288, 1256, 1568, 1909, 3725, 3941
BspHI TCATGA 3 cut(s) 1516, 2535, 3670
BspMAI CTGCAG 2 cut(s) 1926, 2479
BspPI GGATC 6 cut(s) 690, 894, 1431, 1586, 1599, 2962
BspQI GCTCTTC 3 cut(s) 2835, 3195, 3964
BspT104I TTCGAA 1 cut(s) 1667
BsrFI RCCGGY 3 cut(s) 431, 1573, 2540
BsrGI TGTACA 2 cut(s) 2428, 2916
BsrI ACTGG 9 cut(s) 1344, 1583, 1885, 1972, 2785, 2896, 2952, 3395, 3601
BssAI RCCGGY 3 cut(s) 431, 1573, 2540
BssECI CCNNGG 9 cut(s) 396, 403, 678, 993, 994, 2810, 2983, 3060, 3952
BssT1I CCWWGG 5 cut(s) 396, 403, 678, 2810, 2983
Bst2UI CCWGG 6 cut(s) 995, 1538, 2412, 2898, 3062, 3953
Bst6I CTCTTC 8 cut(s) 588, 1140, 1328, 2088, 2835, 3195, 3794, 3964
BstAPI GCANNNNNTGC 3 cut(s) 1409, 1763, 1814
BstAUI TGTACA 2 cut(s) 2428, 2916
BstBAI YACGTR 3 cut(s) 236, 526, 2992
BstBI TTCGAA 1 cut(s) 1667
BstDSI CCRYGG 1 cut(s) 396
BstHHI GCGC 2 cut(s) 877, 1398
BstMAI GTCTC 8 cut(s) 1330, 1395, 2096, 2732, 3015, 3432, 3796, 3937
BstNI CCWGG 6 cut(s) 995, 1538, 2412, 2898, 3062, 3953
BstNSI RCATGY 4 cut(s) 1195, 2907, 3416, 3703
BstSCI CCNGG 7 cut(s) 993, 1536, 2410, 2515, 2896, 3060, 3951
BstSFI CTRYAG 3 cut(s) 1767, 1922, 2475
BstSLI GKGCMC 1 cut(s) 2395
BstV2I GAAGAC 2 cut(s) 1083, 3476
BstX2I RGATCY 7 cut(s) 268, 682, 724, 817, 886, 1591, 2967
BstXI CCANNNNNNTGG 1 cut(s) 2873
BstYI RGATCY 7 cut(s) 268, 682, 724, 817, 886, 1591, 2967
Bsu36I CCTNAGG 1 cut(s) 3136
BsuI GTATCC 3 cut(s) 2440, 3247, 3769
BtgI CCRYGG 1 cut(s) 396
BtgZI GCGATG 1 cut(s) 3309
BtrI CACGTC 4 cut(s) 1106, 1599, 1679, 2057
BtsI GCAGTG 7 cut(s) 1405, 2484, 3403, 3631, 3710, 3723, 3734
CaiI CAGNNNCTG 4 cut(s) 1435, 1763, 1891, 3682
CciI TCATGA 3 cut(s) 1516, 2535, 3670
CfoI GCGC 2 cut(s) 877, 1398
Cfr10I RCCGGY 3 cut(s) 431, 1573, 2540
CseI GACGC 1 cut(s) 2309
CspAI ACCGGT 1 cut(s) 431
CspCI CAANNNNNGTGG 2 cut(s) 2040, 2075
DraI TTTAAA 1 cut(s) 298
DriI GACNNNNNGTC 2 cut(s) 1082, 3445
EaeI YGGCCR 4 cut(s) 675, 1608, 2542, 2949
Eam1104I CTCTTC 8 cut(s) 588, 1140, 1328, 2088, 2835, 3195, 3794, 3964
Eam1105I GACNNNNNGTC 2 cut(s) 1082, 3445
EarI CTCTTC 8 cut(s) 588, 1140, 1328, 2088, 2835, 3195, 3794, 3964
Ecl136II GAGCTC 1 cut(s) 3107
Eco130I CCWWGG 5 cut(s) 396, 403, 678, 2810, 2983
Eco147I AGGCCT 1 cut(s) 1528
Eco24I GRGCYC 5 cut(s) 1038, 1100, 2707, 3109, 3959
Eco32I GATATC 1 cut(s) 2524
Eco47I GGWCC 5 cut(s) 400, 3049, 3166, 3447, 3746
Eco53kI GAGCTC 1 cut(s) 3107
Eco57I CTGAAG 5 cut(s) 72, 834, 1008, 3035, 3488
Eco72I CACGTG 1 cut(s) 526
Eco81I CCTNAGG 1 cut(s) 3136
Eco88I CYCGRG 3 cut(s) 1099, 3303, 3619
EcoICRI GAGCTC 1 cut(s) 3107
EcoO109I RGGNCCY 2 cut(s) 3447, 3746
EcoRI GAATTC 1 cut(s) 1850
EcoRII CCWGG 6 cut(s) 993, 1536, 2410, 2896, 3060, 3951
EcoRV GATATC 1 cut(s) 2524
EcoT14I CCWWGG 5 cut(s) 396, 403, 678, 2810, 2983
EcoT22I ATGCAT 2 cut(s) 952, 2717
EcoT38I GRGCYC 5 cut(s) 1038, 1100, 2707, 3109, 3959
ErhI CCWWGG 5 cut(s) 396, 403, 678, 2810, 2983
Esp3I CGTCTC 1 cut(s) 3432
FalI AAGNNNNNCTT 6 cut(s) 1491, 1523, 1516, 1548, 2426, 2458
FaqI GGGAC 6 cut(s) 802, 1116, 3305, 3401, 3433, 3759
FauI CCCGC 2 cut(s) 1902, 3934
FauNDI CATATG 1 cut(s) 1824
FblI GTMKAC 1 cut(s) 636
FriOI GRGCYC 5 cut(s) 1038, 1100, 2707, 3109, 3959
FspBI CTAG 7 cut(s) 1139, 1637, 2811, 2844, 3206, 3435, 3458
FspI TGCGCA 1 cut(s) 876
GlaI GCGC 2 cut(s) 876, 1397
GsaI CCCAGC 4 cut(s) 2050, 2320, 2877, 3454
HapII CCGG 5 cut(s) 432, 1574, 2516, 2541, 3592
HgaI GACGC 1 cut(s) 2309
HhaI GCGC 2 cut(s) 877, 1398
Hin6I GCGC 2 cut(s) 875, 1396
HinP1I GCGC 2 cut(s) 875, 1396
HincII GTYRAC 1 cut(s) 1682
HindII GTYRAC 1 cut(s) 1682
HinfI GANTC 8 cut(s) 29, 71, 1157, 2216, 3326, 3463, 3679, 3770
HpaII CCGG 5 cut(s) 432, 1574, 2516, 2541, 3592
HphI GGTGA 8 cut(s) 1166, 1196, 1727, 1796, 2126, 2531, 3209, 3326
Hpy166II GTNNAC 7 cut(s) 523, 637, 1682, 2026, 2393, 2803, 2919
Hpy8I GTNNAC 7 cut(s) 523, 637, 1682, 2026, 2393, 2803, 2919
Hpy99I CGWCG 2 cut(s) 2315, 2325
HpyAV CCTTC 4 cut(s) 1160, 1238, 1309, 3010
HspAI GCGC 2 cut(s) 875, 1396
KflI GGGWCCC 1 cut(s) 3746
LguI GCTCTTC 3 cut(s) 2835, 3195, 3964
LmnI GCTCC 6 cut(s) 4, 1033, 1570, 1693, 3104, 3721
MaeI CTAG 7 cut(s) 1139, 1637, 2811, 2844, 3206, 3435, 3458
MfeI CAATTG 1 cut(s) 3231
MflI RGATCY 7 cut(s) 268, 682, 724, 817, 886, 1591, 2967
MhlI GDGCHC 8 cut(s) 135, 1038, 1100, 2395, 2707, 3109, 3631, 3959
MlsI TGGCCA 3 cut(s) 677, 1610, 2951
MluNI TGGCCA 3 cut(s) 677, 1610, 2951
MlyI GAGTC 2 cut(s) 3335, 3779
MmeI TCCRAC 3 cut(s) 525, 1939, 3753
Mox20I TGGCCA 3 cut(s) 677, 1610, 2951
Mph1103I ATGCAT 2 cut(s) 952, 2717
MroXI GAANNNNTTC 2 cut(s) 3069, 3678
MscI TGGCCA 3 cut(s) 677, 1610, 2951
MslI CAYNNNNRTG 3 cut(s) 1752, 2010, 2534
Msp20I TGGCCA 3 cut(s) 677, 1610, 2951
MspA1I CMGCKG 6 cut(s) 248, 1256, 2656, 2663, 3091, 3454
MspI CCGG 5 cut(s) 432, 1574, 2516, 2541, 3592
MspR9I CCNGG 7 cut(s) 995, 1538, 2412, 2517, 2898, 3062, 3953
MunI CAATTG 1 cut(s) 3231
Mva1269I GAATGC 2 cut(s) 267, 493
MvaI CCWGG 6 cut(s) 995, 1538, 2412, 2898, 3062, 3953
NciI CCSGG 1 cut(s) 2517
NcoI CCATGG 1 cut(s) 396
NdeI CATATG 1 cut(s) 1824
NmuCI GTSAC 6 cut(s) 1217, 2149, 2268, 2341, 2479, 3398
NsbI TGCGCA 1 cut(s) 876
NsiI ATGCAT 2 cut(s) 952, 2717
NspI RCATGY 4 cut(s) 1195, 2907, 3416, 3703
NspV TTCGAA 1 cut(s) 1667
PaeI GCATGC 1 cut(s) 1195
PagI TCATGA 3 cut(s) 1516, 2535, 3670
PasI CCCWGGG 1 cut(s) 994
PceI AGGCCT 1 cut(s) 1528
PciI ACATGT 1 cut(s) 2903
PciSI GCTCTTC 3 cut(s) 2835, 3195, 3964
PcsI WCGNNNNNNNCGW 2 cut(s) 1328, 2875
PctI GAATGC 2 cut(s) 267, 493
PdmI GAANNNNTTC 2 cut(s) 3069, 3678
PfeI GAWTC 6 cut(s) 29, 71, 1157, 2216, 3463, 3679
PflMI CCANNNNNTGG 1 cut(s) 529
PfoI TCCNGGA 2 cut(s) 1536, 2410
PinAI ACCGGT 1 cut(s) 431
PleI GAGTC 2 cut(s) 3334, 3778
PmaCI CACGTG 1 cut(s) 526
PmlI CACGTG 1 cut(s) 526
PpsI GAGTC 2 cut(s) 3334, 3778
Ppu21I YACGTR 3 cut(s) 236, 526, 2992
PpuMI RGGWCCY 2 cut(s) 3447, 3746
PscI ACATGT 1 cut(s) 2903
Psp124BI GAGCTC 1 cut(s) 3109
Psp1406I AACGTT 1 cut(s) 1684
Psp5II RGGWCCY 2 cut(s) 3447, 3746
Psp6I CCWGG 6 cut(s) 993, 1536, 2410, 2896, 3060, 3951
PspCI CACGTG 1 cut(s) 526
PspFI CCCAGC 4 cut(s) 2046, 2316, 2873, 3450
PspGI CCWGG 6 cut(s) 993, 1536, 2410, 2896, 3060, 3951
PspPPI RGGWCCY 2 cut(s) 3447, 3746
PsrI GAACNNNNNNTAC 2 cut(s) 2972, 3004
PstI CTGCAG 2 cut(s) 1926, 2479
PstNI CAGNNNCTG 4 cut(s) 1435, 1763, 1891, 3682
PsuI RGATCY 7 cut(s) 268, 682, 724, 817, 886, 1591, 2967
PvuII CAGCTG 4 cut(s) 2656, 2663, 3091, 3454
RseI CAYNNNNRTG 3 cut(s) 1752, 2010, 2534
SacI GAGCTC 1 cut(s) 3109
SapI GCTCTTC 3 cut(s) 2835, 3195, 3964
ScaI AGTACT 2 cut(s) 2193, 2441
SchI GAGTC 2 cut(s) 3335, 3779
ScrFI CCNGG 7 cut(s) 995, 1538, 2412, 2517, 2898, 3062, 3953
SduI GDGCHC 8 cut(s) 135, 1038, 1100, 2395, 2707, 3109, 3631, 3959
SfcI CTRYAG 3 cut(s) 1767, 1922, 2475
SfuI TTCGAA 1 cut(s) 1667
SinI GGWCC 5 cut(s) 400, 3049, 3166, 3447, 3746
SmiMI CAYNNNNRTG 3 cut(s) 1752, 2010, 2534
SmlI CTYRAG 4 cut(s) 107, 142, 3005, 3858
SmoI CTYRAG 4 cut(s) 107, 142, 3005, 3858
SphI GCATGC 1 cut(s) 1195
SseBI AGGCCT 1 cut(s) 1528
SsiI CCGC 8 cut(s) 47, 246, 288, 1256, 1568, 1909, 3725, 3941
SspI AATATT 2 cut(s) 2731, 3070
SspMI CTAG 7 cut(s) 1139, 1637, 2811, 2844, 3206, 3435, 3458
SstI GAGCTC 1 cut(s) 3109
StuI AGGCCT 1 cut(s) 1528
StyD4I CCNGG 7 cut(s) 993, 1536, 2410, 2515, 2896, 3060, 3951
StyI CCWWGG 5 cut(s) 396, 403, 678, 2810, 2983
TaqI TCGA 6 cut(s) 129, 173, 1309, 1667, 1933, 3111
TatI WGTACW 7 cut(s) 412, 2175, 2191, 2428, 2439, 2916, 3696
TauI GCSGC 3 cut(s) 50, 248, 3727
TfiI GAWTC 6 cut(s) 29, 71, 1157, 2216, 3463, 3679
TseFI GTSAC 6 cut(s) 1217, 2149, 2268, 2341, 2479, 3398
Tsp45I GTSAC 6 cut(s) 1217, 2149, 2268, 2341, 2479, 3398
TspGWI ACGGA 1 cut(s) 1337
Van91I CCANNNNNTGG 1 cut(s) 529
VneI GTGCAC 1 cut(s) 2391
VpaK11BI GGWCC 5 cut(s) 400, 3049, 3166, 3447, 3746
XapI RAATTY 9 cut(s) 84, 981, 1850, 1929, 2369, 2496, 3222, 3311, 3480
XceI RCATGY 4 cut(s) 1195, 2907, 3416, 3703
XcmI CCANNNNNNNNNTGG 1 cut(s) 1603
XmaJI CCTAGG 1 cut(s) 2810
XmiI GTMKAC 1 cut(s) 636
XmnI GAANNNNTTC 2 cut(s) 3069, 3678
XspI CTAG 7 cut(s) 1139, 1637, 2811, 2844, 3206, 3435, 3458
ZrmI AGTACT 2 cut(s) 2193, 2441
Zsp2I ATGCAT 2 cut(s) 952, 2717
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.